Details of the Target

General Information of Target

Target ID LDTP11338
Target Name Poly(A) polymerase gamma (PAPOLG)
Gene Name PAPOLG
Gene ID 64895
Synonyms
PAP2; PAPG; Poly(A) polymerase gamma; PAP-gamma; EC 2.7.7.19; Neo-poly(A) polymerase; Neo-PAP; Polynucleotide adenylyltransferase gamma; SRP RNA 3'-adenylating enzyme; Signal recognition particle RNA-adenylating enzyme; SRP RNA-adenylating enzyme
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDARRVPQKDLRVKKNLKKFRYVKLISMETSSSSDDSCDSFASDNFANTRLQSVREGCRT
RSQCRHSGPLRVAMKFPARSTRGATNKKAESRQPSENSVTDSNSDSEDESGMNFLEKRAL
NIKQNKAMLAKLMSELESFPGSFRGRHPLPGSDSQSRRPRRRTFPGVASRRNPERRARPL
TRSRSRILGSLDALPMEEEEEEDKYMLVRKRKTVDGYMNEDDLPRSRRSRSSVTLPHIIR
PVEEITEEELENVCSNSREKIYNRSLGSTCHQCRQKTIDTKTNCRNPDCWGVRGQFCGPC
LRNRYGEEVRDALLDPNWHCPPCRGICNCSFCRQRDGRCATGVLVYLAKYHGFGNVHAYL
KSLKQEFEMQA
Target Bioclass
Enzyme
Family
Poly(A) polymerase family
Subcellular location
Nucleus
Function
Responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.
Uniprot ID
Q9BWT3
Ensemble ID
ENST00000238714.8
HGNC ID
HGNC:14982

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
IPC298 SNV: p.N539K DBIA    Probe Info 
JURKAT SNV: p.A567V .
LS180 Deletion: p.K231NfsTer10 DBIA    Probe Info 
MEWO SNV: p.W389Ter .
MIAPACA2 Deletion: p.A589EfsTer3 DBIA    Probe Info 
OVK18 SNV: p.I459T .
WM793 SNV: p.P301S DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		9.61  LDD0403  [1]
HHS-475
 Probe Info  		Y326(1.07)  LDD0264  [2]
DBIA
 Probe Info  		C530(1.69)  LDD0080  [3]
IA-alkyne
 Probe Info  		C203(0.00); C117(0.00); C604(0.00)  LDD0165  [4]
IPM
 Probe Info  		C530(0.00); C604(0.00); C203(0.00)  LDD0147  [5]
PPMS
 Probe Info  		N.A.  LDD0008  [6]
TFBX
 Probe Info  		N.A.  LDD0148  [5]
HHS-465
 Probe Info  		N.A.  LDD2240  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C530(0.94)  LDD1507  [8]
 LDCM0215  AC10 HEK-293T C530(0.86)  LDD1508  [8]
 LDCM0226  AC11 HEK-293T C530(1.05); C604(1.15)  LDD1509  [8]
 LDCM0237  AC12 HEK-293T C530(0.93)  LDD1510  [8]
 LDCM0259  AC14 HEK-293T C530(1.01); C604(1.24)  LDD1512  [8]
 LDCM0270  AC15 HEK-293T C604(1.00)  LDD1513  [8]
 LDCM0276  AC17 HEK-293T C530(1.11)  LDD1515  [8]
 LDCM0277  AC18 HEK-293T C530(0.86)  LDD1516  [8]
 LDCM0278  AC19 HEK-293T C530(1.15); C604(0.87)  LDD1517  [8]
 LDCM0279  AC2 HEK-293T C530(0.87)  LDD1518  [8]
 LDCM0280  AC20 HEK-293T C530(0.97)  LDD1519  [8]
 LDCM0281  AC21 HEK-293T C530(1.05); C604(0.97)  LDD1520  [8]
 LDCM0282  AC22 HEK-293T C530(1.14); C604(1.03)  LDD1521  [8]
 LDCM0283  AC23 HEK-293T C604(0.92)  LDD1522  [8]
 LDCM0284  AC24 HEK-293T C530(0.97)  LDD1523  [8]
 LDCM0285  AC25 HEK-293T C530(1.09)  LDD1524  [8]
 LDCM0286  AC26 HEK-293T C530(0.96)  LDD1525  [8]
 LDCM0287  AC27 HEK-293T C530(0.95); C604(0.91)  LDD1526  [8]
 LDCM0288  AC28 HEK-293T C530(1.04)  LDD1527  [8]
 LDCM0289  AC29 HEK-293T C530(1.09); C604(0.90)  LDD1528  [8]
 LDCM0290  AC3 HEK-293T C530(0.94); C604(0.79)  LDD1529  [8]
 LDCM0291  AC30 HEK-293T C530(1.09); C604(1.05)  LDD1530  [8]
 LDCM0292  AC31 HEK-293T C604(0.88)  LDD1531  [8]
 LDCM0293  AC32 HEK-293T C530(1.14)  LDD1532  [8]
 LDCM0294  AC33 HEK-293T C530(0.98)  LDD1533  [8]
 LDCM0295  AC34 HEK-293T C530(1.16)  LDD1534  [8]
 LDCM0296  AC35 HEK-293T C530(1.09); C604(1.08)  LDD1535  [8]
 LDCM0297  AC36 HEK-293T C530(0.93)  LDD1536  [8]
 LDCM0298  AC37 HEK-293T C530(1.08); C604(1.00)  LDD1537  [8]
 LDCM0299  AC38 HEK-293T C530(1.15); C604(1.07)  LDD1538  [8]
 LDCM0300  AC39 HEK-293T C604(0.92)  LDD1539  [8]
 LDCM0301  AC4 HEK-293T C530(0.94)  LDD1540  [8]
 LDCM0302  AC40 HEK-293T C530(0.97)  LDD1541  [8]
 LDCM0303  AC41 HEK-293T C530(1.02)  LDD1542  [8]
 LDCM0304  AC42 HEK-293T C530(0.89)  LDD1543  [8]
 LDCM0305  AC43 HEK-293T C530(0.95); C604(0.96)  LDD1544  [8]
 LDCM0306  AC44 HEK-293T C530(1.01)  LDD1545  [8]
 LDCM0307  AC45 HEK-293T C530(1.09); C604(0.98)  LDD1546  [8]
 LDCM0308  AC46 HEK-293T C530(1.13); C604(1.03)  LDD1547  [8]
 LDCM0309  AC47 HEK-293T C604(1.04)  LDD1548  [8]
 LDCM0310  AC48 HEK-293T C530(1.07)  LDD1549  [8]
 LDCM0311  AC49 HEK-293T C530(1.09)  LDD1550  [8]
 LDCM0312  AC5 HEK-293T C530(1.05); C604(0.94)  LDD1551  [8]
 LDCM0313  AC50 HEK-293T C530(0.89)  LDD1552  [8]
 LDCM0314  AC51 HEK-293T C530(0.95); C604(0.93)  LDD1553  [8]
 LDCM0315  AC52 HEK-293T C530(0.92)  LDD1554  [8]
 LDCM0316  AC53 HEK-293T C530(0.99); C604(1.08)  LDD1555  [8]
 LDCM0317  AC54 HEK-293T C530(1.11); C604(0.95)  LDD1556  [8]
 LDCM0318  AC55 HEK-293T C604(0.86)  LDD1557  [8]
 LDCM0319  AC56 HEK-293T C530(1.02)  LDD1558  [8]
 LDCM0320  AC57 HEK-293T C530(0.90)  LDD1559  [8]
 LDCM0321  AC58 HEK-293T C530(1.02)  LDD1560  [8]
 LDCM0322  AC59 HEK-293T C530(1.09); C604(1.22)  LDD1561  [8]
 LDCM0323  AC6 HEK-293T C530(1.02); C604(1.08)  LDD1562  [8]
 LDCM0324  AC60 HEK-293T C530(0.94)  LDD1563  [8]
 LDCM0325  AC61 HEK-293T C530(1.12); C604(1.07)  LDD1564  [8]
 LDCM0326  AC62 HEK-293T C530(1.06); C604(1.25)  LDD1565  [8]
 LDCM0327  AC63 HEK-293T C604(0.93)  LDD1566  [8]
 LDCM0328  AC64 HEK-293T C530(1.14)  LDD1567  [8]
 LDCM0334  AC7 HEK-293T C604(0.95)  LDD1568  [8]
 LDCM0345  AC8 HEK-293T C530(1.15)  LDD1569  [8]
 LDCM0248  AKOS034007472 HEK-293T C530(1.03); C604(1.01)  LDD1511  [8]
 LDCM0356  AKOS034007680 HEK-293T C530(0.96)  LDD1570  [8]
 LDCM0275  AKOS034007705 HEK-293T C530(1.07)  LDD1514  [8]
 LDCM0156  Aniline NCI-H1299 9.61  LDD0403  [1]
 LDCM0367  CL1 HEK-293T C530(0.90)  LDD1571  [8]
 LDCM0368  CL10 HEK-293T C530(1.10); C604(0.89)  LDD1572  [8]
 LDCM0370  CL101 HEK-293T C530(1.09)  LDD1574  [8]
 LDCM0371  CL102 HEK-293T C530(0.95)  LDD1575  [8]
 LDCM0372  CL103 HEK-293T C530(1.08)  LDD1576  [8]
 LDCM0374  CL105 HEK-293T C530(1.15)  LDD1578  [8]
 LDCM0375  CL106 HEK-293T C530(0.96)  LDD1579  [8]
 LDCM0376  CL107 HEK-293T C530(0.92)  LDD1580  [8]
 LDCM0378  CL109 HEK-293T C530(0.95)  LDD1582  [8]
 LDCM0379  CL11 HEK-293T C604(0.80)  LDD1583  [8]
 LDCM0380  CL110 HEK-293T C530(0.79)  LDD1584  [8]
 LDCM0381  CL111 HEK-293T C530(1.05)  LDD1585  [8]
 LDCM0383  CL113 HEK-293T C530(0.86)  LDD1587  [8]
 LDCM0384  CL114 HEK-293T C530(0.90)  LDD1588  [8]
 LDCM0385  CL115 HEK-293T C530(1.07)  LDD1589  [8]
 LDCM0387  CL117 HEK-293T C530(1.04)  LDD1591  [8]
 LDCM0388  CL118 HEK-293T C530(1.10)  LDD1592  [8]
 LDCM0389  CL119 HEK-293T C530(0.93)  LDD1593  [8]
 LDCM0390  CL12 HEK-293T C530(1.09)  LDD1594  [8]
 LDCM0392  CL121 HEK-293T C530(1.11)  LDD1596  [8]
 LDCM0393  CL122 HEK-293T C530(0.96)  LDD1597  [8]
 LDCM0394  CL123 HEK-293T C530(1.01)  LDD1598  [8]
 LDCM0396  CL125 HEK-293T C530(0.87)  LDD1600  [8]
 LDCM0397  CL126 HEK-293T C530(0.90)  LDD1601  [8]
 LDCM0398  CL127 HEK-293T C530(1.08)  LDD1602  [8]
 LDCM0400  CL13 HEK-293T C530(0.89)  LDD1604  [8]
 LDCM0401  CL14 HEK-293T C530(0.93)  LDD1605  [8]
 LDCM0402  CL15 HEK-293T C530(1.01)  LDD1606  [8]
 LDCM0404  CL17 HEK-293T C530(0.89)  LDD1608  [8]
 LDCM0405  CL18 HEK-293T C530(0.90)  LDD1609  [8]
 LDCM0406  CL19 HEK-293T C530(1.08); C604(1.02)  LDD1610  [8]
 LDCM0407  CL2 HEK-293T C530(1.03)  LDD1611  [8]
 LDCM0408  CL20 HEK-293T C530(1.09)  LDD1612  [8]
 LDCM0409  CL21 HEK-293T C530(1.06); C604(0.94)  LDD1613  [8]
 LDCM0410  CL22 HEK-293T C530(1.21); C604(1.00)  LDD1614  [8]
 LDCM0411  CL23 HEK-293T C604(0.99)  LDD1615  [8]
 LDCM0412  CL24 HEK-293T C530(1.27)  LDD1616  [8]
 LDCM0413  CL25 HEK-293T C530(0.86)  LDD1617  [8]
 LDCM0414  CL26 HEK-293T C530(0.93)  LDD1618  [8]
 LDCM0415  CL27 HEK-293T C530(1.03)  LDD1619  [8]
 LDCM0417  CL29 HEK-293T C530(0.98)  LDD1621  [8]
 LDCM0418  CL3 HEK-293T C530(0.98)  LDD1622  [8]
 LDCM0419  CL30 HEK-293T C530(0.89)  LDD1623  [8]
 LDCM0420  CL31 HEK-293T C530(1.06); C604(1.18)  LDD1624  [8]
 LDCM0421  CL32 HEK-293T C530(1.09)  LDD1625  [8]
 LDCM0422  CL33 HEK-293T C530(1.07); C604(0.91)  LDD1626  [8]
 LDCM0423  CL34 HEK-293T C530(1.08); C604(1.00)  LDD1627  [8]
 LDCM0424  CL35 HEK-293T C604(1.15)  LDD1628  [8]
 LDCM0425  CL36 HEK-293T C530(1.01)  LDD1629  [8]
 LDCM0426  CL37 HEK-293T C530(1.03)  LDD1630  [8]
 LDCM0428  CL39 HEK-293T C530(1.02)  LDD1632  [8]
 LDCM0431  CL41 HEK-293T C530(1.10)  LDD1635  [8]
 LDCM0432  CL42 HEK-293T C530(0.90)  LDD1636  [8]
 LDCM0433  CL43 HEK-293T C530(0.85); C604(1.02)  LDD1637  [8]
 LDCM0434  CL44 HEK-293T C530(1.07)  LDD1638  [8]
 LDCM0435  CL45 HEK-293T C530(1.11); C604(0.96)  LDD1639  [8]
 LDCM0436  CL46 HEK-293T C530(1.20); C604(1.17)  LDD1640  [8]
 LDCM0437  CL47 HEK-293T C604(1.24)  LDD1641  [8]
 LDCM0438  CL48 HEK-293T C530(1.08)  LDD1642  [8]
 LDCM0439  CL49 HEK-293T C530(0.97)  LDD1643  [8]
 LDCM0440  CL5 HEK-293T C530(1.04)  LDD1644  [8]
 LDCM0441  CL50 HEK-293T C530(0.98)  LDD1645  [8]
 LDCM0444  CL53 HEK-293T C530(1.05)  LDD1647  [8]
 LDCM0445  CL54 HEK-293T C530(0.80)  LDD1648  [8]
 LDCM0446  CL55 HEK-293T C530(1.02); C604(0.95)  LDD1649  [8]
 LDCM0447  CL56 HEK-293T C530(1.01)  LDD1650  [8]
 LDCM0448  CL57 HEK-293T C530(1.07); C604(1.07)  LDD1651  [8]
 LDCM0449  CL58 HEK-293T C530(1.37); C604(1.08)  LDD1652  [8]
 LDCM0450  CL59 HEK-293T C604(1.10)  LDD1653  [8]
 LDCM0451  CL6 HEK-293T C530(0.93)  LDD1654  [8]
 LDCM0452  CL60 HEK-293T C530(1.16)  LDD1655  [8]
 LDCM0453  CL61 HEK-293T C530(0.95)  LDD1656  [8]
 LDCM0454  CL62 HEK-293T C530(1.04)  LDD1657  [8]
 LDCM0455  CL63 HEK-293T C530(0.98)  LDD1658  [8]
 LDCM0457  CL65 HEK-293T C530(0.94)  LDD1660  [8]
 LDCM0458  CL66 HEK-293T C530(0.85)  LDD1661  [8]
 LDCM0459  CL67 HEK-293T C530(1.06); C604(1.00)  LDD1662  [8]
 LDCM0460  CL68 HEK-293T C530(1.14)  LDD1663  [8]
 LDCM0461  CL69 HEK-293T C530(1.25); C604(1.11)  LDD1664  [8]
 LDCM0462  CL7 HEK-293T C530(0.97); C604(0.92)  LDD1665  [8]
 LDCM0463  CL70 HEK-293T C530(1.07); C604(1.19)  LDD1666  [8]
 LDCM0464  CL71 HEK-293T C604(1.08)  LDD1667  [8]
 LDCM0465  CL72 HEK-293T C530(1.07)  LDD1668  [8]
 LDCM0466  CL73 HEK-293T C530(0.85)  LDD1669  [8]
 LDCM0467  CL74 HEK-293T C530(0.85)  LDD1670  [8]
 LDCM0470  CL77 HEK-293T C530(0.94)  LDD1673  [8]
 LDCM0471  CL78 HEK-293T C530(0.94)  LDD1674  [8]
 LDCM0472  CL79 HEK-293T C530(1.03); C604(0.95)  LDD1675  [8]
 LDCM0473  CL8 HEK-293T C530(0.89)  LDD1676  [8]
 LDCM0474  CL80 HEK-293T C530(0.92)  LDD1677  [8]
 LDCM0475  CL81 HEK-293T C530(1.12); C604(0.94)  LDD1678  [8]
 LDCM0476  CL82 HEK-293T C530(1.38); C604(1.05)  LDD1679  [8]
 LDCM0477  CL83 HEK-293T C604(0.89)  LDD1680  [8]
 LDCM0478  CL84 HEK-293T C530(1.01)  LDD1681  [8]
 LDCM0479  CL85 HEK-293T C530(0.92)  LDD1682  [8]
 LDCM0480  CL86 HEK-293T C530(0.85)  LDD1683  [8]
 LDCM0481  CL87 HEK-293T C530(0.92)  LDD1684  [8]
 LDCM0483  CL89 HEK-293T C530(0.99)  LDD1686  [8]
 LDCM0484  CL9 HEK-293T C530(1.07); C604(0.94)  LDD1687  [8]
 LDCM0485  CL90 HEK-293T C530(0.74)  LDD1688  [8]
 LDCM0486  CL91 HEK-293T C530(0.97); C604(0.96)  LDD1689  [8]
 LDCM0487  CL92 HEK-293T C530(0.96)  LDD1690  [8]
 LDCM0488  CL93 HEK-293T C530(1.00); C604(0.86)  LDD1691  [8]
 LDCM0489  CL94 HEK-293T C530(1.20); C604(1.07)  LDD1692  [8]
 LDCM0490  CL95 HEK-293T C604(1.00)  LDD1693  [8]
 LDCM0491  CL96 HEK-293T C530(1.02)  LDD1694  [8]
 LDCM0492  CL97 HEK-293T C530(0.89)  LDD1695  [8]
 LDCM0493  CL98 HEK-293T C530(0.93)  LDD1696  [8]
 LDCM0494  CL99 HEK-293T C530(1.00)  LDD1697  [8]
 LDCM0495  E2913 HEK-293T C530(1.04)  LDD1698  [8]
 LDCM0468  Fragment33 HEK-293T C530(1.09)  LDD1671  [8]
 LDCM0427  Fragment51 HEK-293T C530(0.88)  LDD1631  [8]
 LDCM0116  HHS-0101 DM93 Y326(1.07)  LDD0264  [2]
 LDCM0117  HHS-0201 DM93 Y326(5.23)  LDD0265  [2]
 LDCM0118  HHS-0301 DM93 Y326(2.58)  LDD0266  [2]
 LDCM0119  HHS-0401 DM93 Y326(2.80)  LDD0267  [2]
 LDCM0120  HHS-0701 DM93 Y326(11.59)  LDD0268  [2]
 LDCM0022  KB02 HCT 116 C530(1.69)  LDD0080  [3]
 LDCM0023  KB03 HCT 116 C530(1.36)  LDD0081  [3]
 LDCM0024  KB05 HCT 116 C530(1.52)  LDD0082  [3]

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
3 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
6 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
7 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
8 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402