Details of the Target

General Information of Target

Target ID LDTP11304
Target Name Mitochondrial import inner membrane translocase subunit Tim21 (TIMM21)
Gene Name TIMM21
Gene ID 29090
Synonyms
C18orf55; TIM21; Mitochondrial import inner membrane translocase subunit Tim21; TIM21-like protein, mitochondrial
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPVKRLREVVSQNHGDHLVLLKDELPCVPPALSANKRLPVGTGTSLNGTSRGSSDLTSAR
NCYQPLLENPMVSESDFSKDVAVQVLPLDKIEENNKQKANDIFISQYTMGQKDALRTVLK
QKAQSMPVFKEVKVHLLEDAGIEKDAVTQETRISPSGIDSATTVAAATAAAIATAAPLIK
VQSDLEAKVNSVTELLSKLQETDKHLQRVTEQQTSIQRKQEKLHCHDHEKQMNVFMEQHI
RHLEKLQQQQIDIQTHFISAALKTSSFQPVSMPSSRAVEKYSVKPEHPNLGSCNPSLYNT
FASKQAPLKEVEDTSFDKQKSPLETPAPRRFAPVPVSRDDELSKRENLLEEKENMEVSCH
RGNVRLLEQILNNNDSLTRKSESSNTTSLTRSKIGWTPEKTNRFPSCEELETTKVTMQKS
DDVLHDLGQKEKETNSMVQPKESLSMLKLPDLPQNSVKLQTTNTTRSVLKDAEKILRGVQ
NNKKVLEENLEAIIRAKDGAAMYSLINALSTNREMSEKIRIRKTVDEWIKTISAEIQDEL
SRTDYEQKRFDQKNQRTKKGQNMTKDIRTNTQDKTVNKSVIPRKHSQKQIEEHFRNLPMR
GMPASSLQKERKEGLLKATTVIQDEDYMLQVYGKPVYQGHRSTLKKGPYLRFNSPSPKSR
PQRPKVIERVKGTKVKSIRTQTDFYATKPKKMDSKMKHSVPVLPHGDQQYLFSPSREMPT
FSGTLEGHLIPMAILLGQTQSNSDTMPPAGVIVSKPHPVTVTTSIPPSSRKVETGVKKPN
IAIVEMKSEKKDPPQLTVQVLPSVDIDSISNSSADVLSPLSSPKEASLPPVQTWIKTPEI
MKVDEEEVKFPGTNFDEIIDVIQEEEKCDEIPDSEPILEFNRSVKADSTKYNGPPFPPVA
STFQPTADILDKVIERKETLENSLIQWVEQEIMSRIISGLFPVQQQIAPSISVSVSETSE
PLTSDIVEGTSSGALQLFVDAGVPVNSNVIKHFVNEALAETIAVMLGDREAKKQGPVATG
VSGDASTNETYLPARVCTPLPTPQPTPPCSPSSPAKECVLVKTPDSSPCDSDHDMAFPVK
EICAEKGDDMPAIMLVNTPTVTPTTTPPPAAAVFTPTLSDISIDKLKVSSPELPKPWGDG
DLPLEEENPNSPQEELHPRAIVMSVAKDEEPESMDFPAQPPPPEPVPFMPFPAGTKAPSP
SQMPGSDSSTLESTLSVTVTETETLDKPISEGEILFSCGQKLAPKILEDIGLYLTNLNDS
LSSTLHDAVEMEDDPPSEGQVIRMSHKKFHADAILSFAKQNQESAVSQQAVYHSEDLENS
VGELSEGQRPQLTAAAENILMGHSLYMQPPVTNTQSLDQQCDPKPLSRQFDTVSGSIYED
SCASHGPMSLGELELEPNSKLVLPTTLLTAQENDVNLPVAAEDFSQYQLKQNQDVKQVEH
KPSQSYLRVRNKSDIAPSQQQVSPGDMDRTQIELNPYLTCVFSGGKAVPLSASQMPPAKM
SVMLPSVNLEDCSQSLSLSTMQEDMESSGADTF
Target Bioclass
Other
Family
TIM21 family
Subcellular location
Mitochondrion membrane
Function
Participates in the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Also required for assembly of mitochondrial respiratory chain complex I and complex IV as component of the MITRAC (mitochondrial translation regulation assembly intermediate of cytochrome c oxidase complex) complex. Probably shuttles between the presequence translocase and respiratory-chain assembly intermediates in a process that promotes incorporation of early nuclear-encoded subunits into these complexes.
Uniprot ID
Q9BVV7
Ensemble ID
ENST00000169551.11
HGNC ID
HGNC:25010

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
C-Sul
 Probe Info  		2.86  LDD0066  [2]
STPyne
 Probe Info  		K148(2.56)  LDD0277  [3]
BTD
 Probe Info  		C149(1.84)  LDD1700  [4]
EA-probe
 Probe Info  		N.A.  LDD0440  [5]
IA-alkyne
 Probe Info  		C192(5.68)  LDD1704  [6]
Acrolein
 Probe Info  		N.A.  LDD0223  [7]
IPM
 Probe Info  		N.A.  LDD0025  [8]
TFBX
 Probe Info  		N.A.  LDD0148  [8]
AOyne
 Probe Info  		15.00  LDD0443  [9]
NAIA_5
 Probe Info  		N.A.  LDD2223  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C149(0.84)  LDD2117  [4]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C149(1.12)  LDD2152  [4]
 LDCM0156  Aniline NCI-H1299 12.30  LDD0403  [1]
 LDCM0498  BS-3668 MDA-MB-231 C149(0.62)  LDD2091  [4]
 LDCM0632  CL-Sc Hep-G2 C192(20.00)  LDD2227  [10]
 LDCM0175  Ethacrynic acid HeLa N.A.  LDD0440  [5]
 LDCM0149  GA MCF-7 C192(3.66)  LDD0379  [11]
 LDCM0022  KB02 T cell-activated C192(5.68)  LDD1704  [6]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C149(0.47)  LDD2121  [4]
 LDCM0109  NEM HeLa N.A.  LDD0223  [7]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C149(0.60)  LDD2089  [4]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C149(1.03)  LDD2093  [4]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C149(1.16)  LDD2097  [4]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C149(0.91)  LDD2099  [4]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C149(1.11)  LDD2107  [4]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C149(0.24)  LDD2108  [4]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C149(0.57)  LDD2109  [4]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C149(1.05)  LDD2111  [4]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C149(3.09)  LDD2119  [4]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C149(0.71)  LDD2125  [4]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C149(0.97)  LDD2127  [4]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C149(1.06)  LDD2129  [4]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C149(1.16)  LDD2135  [4]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C149(1.12)  LDD2136  [4]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C149(0.91)  LDD2137  [4]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C149(1.84)  LDD1700  [4]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C149(0.75)  LDD2140  [4]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C149(2.60)  LDD2144  [4]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C149(0.70)  LDD2150  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cytochrome c oxidase subunit 1 (MT-CO1) Heme-copper respiratory oxidase family P00395
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Mitochondrial import inner membrane translocase subunit Tim23 (TIMM23) Tim17/Tim22/Tim23 family O14925
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ubiquilin-2 (UBQLN2) . Q9UHD9

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 Chemoproteomic Profiling Reveals Ethacrynic Acid Targets Adenine Nucleotide Translocases to Impair Mitochondrial Function. Mol Pharm. 2018 Jun 4;15(6):2413-2422. doi: 10.1021/acs.molpharmaceut.8b00250. Epub 2018 May 15.
6 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
7 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
8 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
9 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
10 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
11 Combined Omics Approach Identifies Gambogic Acid and Related Xanthones as Covalent Inhibitors of the Serine Palmitoyltransferase Complex. Cell Chem Biol. 2020 May 21;27(5):586-597.e12. doi: 10.1016/j.chembiol.2020.03.008. Epub 2020 Apr 23.