Details of the Target

General Information of Target

Target ID LDTP11244
Target Name Coiled-coil domain-containing protein 28B (CCDC28B)
Gene Name CCDC28B
Gene ID 79140
Synonyms
Coiled-coil domain-containing protein 28B
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MESTLGAGIVIAEALQNQLAWLENVWLWITFLGDPKILFLFYFPAAYYASRRVGIAVLWI
SLITEWLNLIFKWFLFGDRPFWWVHESGYYSQAPAQVHQFPSSCETGPGSPSGHCMITGA
ALWPIMTALSSQVATRARSRWVRVMPSLAYCTFLLAVGLSRIFILAHFPHQVLAGLITGA
VLGWLMTPRVPMERELSFYGLTALALMLGTSLIYWTLFTLGLDLSWSISLAFKWCERPEW
IHVDSRPFASLSRDSGAALGLGIALHSPCYAQVRRAQLGNGQKIACLVLAMGLLGPLDWL
GHPPQISLFYIFNFLKYTLWPCLVLALVPWAVHMFSAQEAPPIHSS
Target Bioclass
Other
Subcellular location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Function
Involved in ciliogenesis. Regulates cilia length through its interaction with MAPKAP1/SIN1 but independently of mTORC2 complex. Modulates mTORC2 complex assembly and function, possibly enhances AKT1 phosphorylation. Does not seem to modulate assembly and function of mTORC1 complex.
Uniprot ID
Q9BUN5
Ensemble ID
ENST00000373602.10
HGNC ID
HGNC:28163

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
JURKAT SNV: p.H133Y .
LS180 SNV: p.S9N .
MEWO SNV: p.E143K .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C114(3.45)  LDD3410  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [2]
NAIA_4
 Probe Info  		N.A.  LDD2226  [3]
IPM
 Probe Info  		C13(0.00); C114(0.00)  LDD2156  [4]
VSF
 Probe Info  		N.A.  LDD0007  [5]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C114(0.95)  LDD1507  [7]
 LDCM0215  AC10 HEK-293T C114(0.89)  LDD1508  [7]
 LDCM0237  AC12 HEK-293T C114(0.99)  LDD1510  [7]
 LDCM0276  AC17 HEK-293T C114(1.07)  LDD1515  [7]
 LDCM0277  AC18 HEK-293T C114(0.92)  LDD1516  [7]
 LDCM0279  AC2 HEK-293T C114(0.83)  LDD1518  [7]
 LDCM0280  AC20 HEK-293T C114(1.07)  LDD1519  [7]
 LDCM0281  AC21 HEK-293T C114(0.96)  LDD1520  [7]
 LDCM0284  AC24 HEK-293T C114(1.20)  LDD1523  [7]
 LDCM0285  AC25 HEK-293T C114(0.97)  LDD1524  [7]
 LDCM0286  AC26 HEK-293T C114(0.91)  LDD1525  [7]
 LDCM0288  AC28 HEK-293T C114(0.95)  LDD1527  [7]
 LDCM0289  AC29 HEK-293T C114(0.92)  LDD1528  [7]
 LDCM0293  AC32 HEK-293T C114(1.16)  LDD1532  [7]
 LDCM0294  AC33 HEK-293T C114(1.22)  LDD1533  [7]
 LDCM0295  AC34 HEK-293T C114(0.94)  LDD1534  [7]
 LDCM0297  AC36 HEK-293T C114(1.03)  LDD1536  [7]
 LDCM0298  AC37 HEK-293T C114(0.95)  LDD1537  [7]
 LDCM0301  AC4 HEK-293T C114(0.96)  LDD1540  [7]
 LDCM0302  AC40 HEK-293T C114(0.98)  LDD1541  [7]
 LDCM0303  AC41 HEK-293T C114(1.00)  LDD1542  [7]
 LDCM0304  AC42 HEK-293T C114(0.88)  LDD1543  [7]
 LDCM0306  AC44 HEK-293T C114(0.85)  LDD1545  [7]
 LDCM0307  AC45 HEK-293T C114(1.19)  LDD1546  [7]
 LDCM0310  AC48 HEK-293T C114(1.11)  LDD1549  [7]
 LDCM0311  AC49 HEK-293T C114(1.06)  LDD1550  [7]
 LDCM0312  AC5 HEK-293T C114(1.17)  LDD1551  [7]
 LDCM0313  AC50 HEK-293T C114(0.90)  LDD1552  [7]
 LDCM0315  AC52 HEK-293T C114(0.95)  LDD1554  [7]
 LDCM0316  AC53 HEK-293T C114(1.07)  LDD1555  [7]
 LDCM0319  AC56 HEK-293T C114(1.26)  LDD1558  [7]
 LDCM0320  AC57 HEK-293T C114(0.99)  LDD1559  [7]
 LDCM0321  AC58 HEK-293T C114(0.92)  LDD1560  [7]
 LDCM0324  AC60 HEK-293T C114(1.08)  LDD1563  [7]
 LDCM0325  AC61 HEK-293T C114(0.91)  LDD1564  [7]
 LDCM0328  AC64 HEK-293T C114(1.04)  LDD1567  [7]
 LDCM0345  AC8 HEK-293T C114(0.98)  LDD1569  [7]
 LDCM0248  AKOS034007472 HEK-293T C114(1.03)  LDD1511  [7]
 LDCM0356  AKOS034007680 HEK-293T C114(1.13)  LDD1570  [7]
 LDCM0275  AKOS034007705 HEK-293T C114(0.98)  LDD1514  [7]
 LDCM0369  CL100 HEK-293T C114(1.18)  LDD1573  [7]
 LDCM0373  CL104 HEK-293T C114(1.16)  LDD1577  [7]
 LDCM0377  CL108 HEK-293T C114(1.02)  LDD1581  [7]
 LDCM0382  CL112 HEK-293T C114(1.02)  LDD1586  [7]
 LDCM0386  CL116 HEK-293T C114(1.42)  LDD1590  [7]
 LDCM0390  CL12 HEK-293T C114(1.00)  LDD1594  [7]
 LDCM0391  CL120 HEK-293T C114(1.14)  LDD1595  [7]
 LDCM0395  CL124 HEK-293T C114(0.95)  LDD1599  [7]
 LDCM0399  CL128 HEK-293T C114(1.21)  LDD1603  [7]
 LDCM0403  CL16 HEK-293T C114(1.26)  LDD1607  [7]
 LDCM0404  CL17 HEK-293T C114(1.18)  LDD1608  [7]
 LDCM0405  CL18 HEK-293T C114(0.99)  LDD1609  [7]
 LDCM0408  CL20 HEK-293T C114(0.89)  LDD1612  [7]
 LDCM0409  CL21 HEK-293T C114(1.05)  LDD1613  [7]
 LDCM0412  CL24 HEK-293T C114(1.00)  LDD1616  [7]
 LDCM0416  CL28 HEK-293T C114(1.23)  LDD1620  [7]
 LDCM0417  CL29 HEK-293T C114(1.04)  LDD1621  [7]
 LDCM0419  CL30 HEK-293T C114(0.93)  LDD1623  [7]
 LDCM0421  CL32 HEK-293T C114(0.99)  LDD1625  [7]
 LDCM0422  CL33 HEK-293T C114(1.13)  LDD1626  [7]
 LDCM0425  CL36 HEK-293T C114(1.05)  LDD1629  [7]
 LDCM0429  CL4 HEK-293T C114(1.17)  LDD1633  [7]
 LDCM0430  CL40 HEK-293T C114(1.41)  LDD1634  [7]
 LDCM0431  CL41 HEK-293T C114(1.05)  LDD1635  [7]
 LDCM0432  CL42 HEK-293T C114(0.86)  LDD1636  [7]
 LDCM0434  CL44 HEK-293T C114(0.93)  LDD1638  [7]
 LDCM0435  CL45 HEK-293T C114(1.10)  LDD1639  [7]
 LDCM0438  CL48 HEK-293T C114(1.17)  LDD1642  [7]
 LDCM0440  CL5 HEK-293T C114(1.06)  LDD1644  [7]
 LDCM0443  CL52 HEK-293T C114(1.20)  LDD1646  [7]
 LDCM0444  CL53 HEK-293T C114(1.20)  LDD1647  [7]
 LDCM0445  CL54 HEK-293T C114(0.83)  LDD1648  [7]
 LDCM0447  CL56 HEK-293T C114(1.06)  LDD1650  [7]
 LDCM0448  CL57 HEK-293T C114(1.22)  LDD1651  [7]
 LDCM0451  CL6 HEK-293T C114(0.90)  LDD1654  [7]
 LDCM0452  CL60 HEK-293T C114(1.36)  LDD1655  [7]
 LDCM0456  CL64 HEK-293T C114(1.22)  LDD1659  [7]
 LDCM0457  CL65 HEK-293T C114(1.15)  LDD1660  [7]
 LDCM0458  CL66 HEK-293T C114(0.90)  LDD1661  [7]
 LDCM0460  CL68 HEK-293T C114(0.96)  LDD1663  [7]
 LDCM0461  CL69 HEK-293T C114(1.10)  LDD1664  [7]
 LDCM0465  CL72 HEK-293T C114(1.39)  LDD1668  [7]
 LDCM0469  CL76 HEK-293T C114(1.02)  LDD1672  [7]
 LDCM0470  CL77 HEK-293T C114(0.87)  LDD1673  [7]
 LDCM0471  CL78 HEK-293T C114(0.88)  LDD1674  [7]
 LDCM0473  CL8 HEK-293T C114(1.19)  LDD1676  [7]
 LDCM0474  CL80 HEK-293T C114(0.94)  LDD1677  [7]
 LDCM0475  CL81 HEK-293T C114(0.97)  LDD1678  [7]
 LDCM0478  CL84 HEK-293T C114(0.88)  LDD1681  [7]
 LDCM0482  CL88 HEK-293T C114(1.23)  LDD1685  [7]
 LDCM0483  CL89 HEK-293T C114(1.14)  LDD1686  [7]
 LDCM0484  CL9 HEK-293T C114(0.97)  LDD1687  [7]
 LDCM0485  CL90 HEK-293T C114(0.80)  LDD1688  [7]
 LDCM0487  CL92 HEK-293T C114(1.08)  LDD1690  [7]
 LDCM0488  CL93 HEK-293T C114(1.06)  LDD1691  [7]
 LDCM0491  CL96 HEK-293T C114(0.90)  LDD1694  [7]
 LDCM0625  F8 Ramos C13(0.53); C114(1.77)  LDD2187  [8]
 LDCM0572  Fragment10 Ramos C13(2.57)  LDD2189  [8]
 LDCM0573  Fragment11 Ramos C13(0.95); C114(2.12)  LDD2190  [8]
 LDCM0574  Fragment12 Ramos C13(2.00)  LDD2191  [8]
 LDCM0575  Fragment13 Ramos C13(0.90)  LDD2192  [8]
 LDCM0576  Fragment14 Ramos C13(0.52); C114(0.71)  LDD2193  [8]
 LDCM0579  Fragment20 Ramos C13(1.78)  LDD2194  [8]
 LDCM0580  Fragment21 Ramos C13(1.27)  LDD2195  [8]
 LDCM0582  Fragment23 Ramos C13(1.53)  LDD2196  [8]
 LDCM0578  Fragment27 Ramos C13(0.80)  LDD2197  [8]
 LDCM0586  Fragment28 Ramos C13(0.83); C114(0.69)  LDD2198  [8]
 LDCM0588  Fragment30 Ramos C13(1.34)  LDD2199  [8]
 LDCM0589  Fragment31 Ramos C13(0.92)  LDD2200  [8]
 LDCM0590  Fragment32 Ramos C13(4.09)  LDD2201  [8]
 LDCM0468  Fragment33 Ramos C13(1.31)  LDD2202  [8]
 LDCM0596  Fragment38 Ramos C13(1.03)  LDD2203  [8]
 LDCM0566  Fragment4 Ramos C13(0.93); C114(2.72)  LDD2184  [8]
 LDCM0610  Fragment52 Ramos C13(1.21)  LDD2204  [8]
 LDCM0614  Fragment56 Ramos C13(1.27)  LDD2205  [8]
 LDCM0569  Fragment7 Ramos C13(0.80); C114(1.08)  LDD2186  [8]
 LDCM0571  Fragment9 Ramos C13(1.44)  LDD2188  [8]
 LDCM0022  KB02 HEK-293T C114(0.93)  LDD1492  [7]
 LDCM0023  KB03 HEK-293T C114(0.90)  LDD1497  [7]
 LDCM0024  KB05 RPMI-7951 C114(3.45)  LDD3410  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
5'-AMP-activated protein kinase subunit beta-2 (PRKAB2) 5'-AMP-activated protein kinase beta subunit family O43741
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Bardet-Biedl syndrome 4 protein (BBS4) BBS4 family Q96RK4
Sodium channel modifier 1 (SCNM1) . Q9BWG6
Other
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
ATR-interacting protein (ATRIP) ATRIP family Q8WXE1
Growth factor receptor-bound protein 2 (GRB2) GRB2/sem-5/DRK family P62993
Armadillo repeat-containing protein 7 (ARMC7) . Q9H6L4
Enkurin domain-containing protein 1 (ENKD1) . Q9H0I2
Ubiquitin-like protein 5 (UBL5) . Q9BZL1

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
5 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
6 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
7 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
8 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578