Details of the Target

General Information of Target

Target ID LDTP10024
Target Name KICSTOR complex protein ITFG2 (ITFG2)
Gene Name ITFG2
Gene ID 55846
Synonyms
KICSTOR complex protein ITFG2; Integrin-alpha FG-GAP repeat-containing protein 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEKPRSIEETPSSEPMEEEEDDDLELFGGYDSFRSYNSSVGSESSSYLEESSEAENEDRE
AGELPTSPLHLLSPGTPRSLDGSGSEPAVCEMCGIVGTREAFFSKTKRFCSVSCSRSYSS
NSKKASILARLQGKPPTKKAKVLHKAAWSAKIGAFLHSQGTGQLADGTPTGQDALVLGFD
WGKFLKDHSYKAAPVSCFKHVPLYDQWEDVMKGMKVEVLNSDAVLPSRVYWIASVIQTAG
YRVLLRYEGFENDASHDFWCNLGTVDVHPIGWCAINSKILVPPRTIHAKFTDWKGYLMKR
LVGSRTLPVDFHIKMVESMKYPFRQGMRLEVVDKSQVSRTRMAVVDTVIGGRLRLLYEDG
DSDDDFWCHMWSPLIHPVGWSRRVGHGIKMSERRSDMAHHPTFRKIYCDAVPYLFKKVRA
VYTEGGWFEEGMKLEAIDPLNLGNICVATVCKVLLDGYLMICVDGGPSTDGLDWFCYHAS
SHAIFPATFCQKNDIELTPPKGYEAQTFNWENYLEKTKSKAAPSRLFNMDCPNHGFKVGM
KLEAVDLMEPRLICVATVKRVVHRLLSIHFDGWDSEYDQWVDCESPDIYPVGWCELTGYQ
LQPPVAAEPATPLKAKEATKKKKKQFGKKRKRIPPTKTRPLRQGSKKPLLEDDPQGARKI
SSEPVPGEIIAVRVKEEHLDVASPDKASSPELPVSVENIKQETDD
Target Bioclass
Other
Subcellular location
Lysosome membrane
Function
As part of the KICSTOR complex functions in the amino acid-sensing branch of the TORC1 signaling pathway. Recruits, in an amino acid-independent manner, the GATOR1 complex to the lysosomal membranes and allows its interaction with GATOR2 and the RAG GTPases. Functions upstream of the RAG GTPases and is required to negatively regulate mTORC1 signaling in absence of amino acids. In absence of the KICSTOR complex mTORC1 is constitutively localized to the lysosome and activated. The KICSTOR complex is also probably involved in the regulation of mTORC1 by glucose.
Uniprot ID
Q969R8
Ensemble ID
ENST00000228799.7
HGNC ID
HGNC:30879

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.C279Y .
HCC15 SNV: p.F155V .
Ishikawa (Heraklio) 02 ER SNV: p.N375S .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C140(2.62)  LDD3319  [1]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [2]
IA-alkyne
 Probe Info  		C438(0.00); C140(0.00)  LDD0036  [2]
Lodoacetamide azide
 Probe Info  		C24(0.00); C279(0.00); C438(0.00)  LDD0037  [2]
NAIA_4
 Probe Info  		N.A.  LDD2226  [3]
NAIA_5
 Probe Info  		N.A.  LDD2223  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C438(1.07)  LDD1507  [4]
 LDCM0215  AC10 HEK-293T C438(1.02)  LDD1508  [4]
 LDCM0237  AC12 HEK-293T C438(1.10)  LDD1510  [4]
 LDCM0259  AC14 HEK-293T C438(1.07)  LDD1512  [4]
 LDCM0270  AC15 HEK-293T C438(0.93)  LDD1513  [4]
 LDCM0276  AC17 PaTu 8988t C140(1.25)  LDD1155  [5]
 LDCM0277  AC18 PaTu 8988t C140(1.20)  LDD1156  [5]
 LDCM0278  AC19 PaTu 8988t C140(1.23)  LDD1157  [5]
 LDCM0279  AC2 HEK-293T C438(0.88)  LDD1518  [4]
 LDCM0280  AC20 PaTu 8988t C140(1.35)  LDD1159  [5]
 LDCM0281  AC21 PaTu 8988t C140(1.40)  LDD1160  [5]
 LDCM0282  AC22 PaTu 8988t C140(1.39)  LDD1161  [5]
 LDCM0283  AC23 PaTu 8988t C140(1.40)  LDD1162  [5]
 LDCM0284  AC24 PaTu 8988t C140(1.34)  LDD1163  [5]
 LDCM0285  AC25 HEK-293T C438(1.12)  LDD1524  [4]
 LDCM0286  AC26 HEK-293T C438(0.86)  LDD1525  [4]
 LDCM0288  AC28 HEK-293T C438(1.05)  LDD1527  [4]
 LDCM0289  AC29 HEK-293T C438(1.04)  LDD1528  [4]
 LDCM0291  AC30 HEK-293T C438(1.09)  LDD1530  [4]
 LDCM0292  AC31 HEK-293T C438(0.86)  LDD1531  [4]
 LDCM0293  AC32 HEK-293T C438(1.00)  LDD1532  [4]
 LDCM0294  AC33 HEK-293T C438(1.32)  LDD1533  [4]
 LDCM0295  AC34 HEK-293T C438(1.05)  LDD1534  [4]
 LDCM0297  AC36 HEK-293T C438(0.99)  LDD1536  [4]
 LDCM0298  AC37 HEK-293T C438(1.10)  LDD1537  [4]
 LDCM0299  AC38 HEK-293T C438(1.13)  LDD1538  [4]
 LDCM0300  AC39 HEK-293T C438(1.01)  LDD1539  [4]
 LDCM0301  AC4 HEK-293T C438(0.98)  LDD1540  [4]
 LDCM0302  AC40 HEK-293T C438(1.08)  LDD1541  [4]
 LDCM0303  AC41 HEK-293T C438(1.01)  LDD1542  [4]
 LDCM0304  AC42 HEK-293T C438(1.02)  LDD1543  [4]
 LDCM0306  AC44 HEK-293T C438(1.00)  LDD1545  [4]
 LDCM0307  AC45 HEK-293T C438(1.06)  LDD1546  [4]
 LDCM0308  AC46 HEK-293T C438(1.04)  LDD1547  [4]
 LDCM0309  AC47 HEK-293T C438(1.00)  LDD1548  [4]
 LDCM0310  AC48 HEK-293T C438(1.05)  LDD1549  [4]
 LDCM0311  AC49 HEK-293T C438(1.14)  LDD1550  [4]
 LDCM0312  AC5 HEK-293T C438(1.03)  LDD1551  [4]
 LDCM0313  AC50 HEK-293T C438(0.99)  LDD1552  [4]
 LDCM0315  AC52 HEK-293T C438(1.03)  LDD1554  [4]
 LDCM0316  AC53 HEK-293T C438(1.09)  LDD1555  [4]
 LDCM0317  AC54 HEK-293T C438(1.02)  LDD1556  [4]
 LDCM0318  AC55 HEK-293T C438(0.97)  LDD1557  [4]
 LDCM0319  AC56 HEK-293T C438(1.12)  LDD1558  [4]
 LDCM0320  AC57 HEK-293T C438(0.94)  LDD1559  [4]
 LDCM0321  AC58 HEK-293T C438(0.96)  LDD1560  [4]
 LDCM0323  AC6 HEK-293T C438(1.04)  LDD1562  [4]
 LDCM0324  AC60 HEK-293T C438(1.07)  LDD1563  [4]
 LDCM0325  AC61 HEK-293T C438(1.06)  LDD1564  [4]
 LDCM0326  AC62 HEK-293T C438(1.07)  LDD1565  [4]
 LDCM0327  AC63 HEK-293T C438(1.00)  LDD1566  [4]
 LDCM0328  AC64 HEK-293T C438(1.05)  LDD1567  [4]
 LDCM0334  AC7 HEK-293T C438(1.00)  LDD1568  [4]
 LDCM0345  AC8 HEK-293T C438(1.32)  LDD1569  [4]
 LDCM0248  AKOS034007472 HEK-293T C438(1.12)  LDD1511  [4]
 LDCM0356  AKOS034007680 HEK-293T C438(1.15)  LDD1570  [4]
 LDCM0275  AKOS034007705 HEK-293T C438(1.02)  LDD1514  [4]
 LDCM0367  CL1 HEK-293T C438(0.43)  LDD1571  [4]
 LDCM0368  CL10 HEK-293T C438(1.30)  LDD1572  [4]
 LDCM0370  CL101 HEK-293T C438(0.88)  LDD1574  [4]
 LDCM0372  CL103 HEK-293T C438(0.91)  LDD1576  [4]
 LDCM0374  CL105 PaTu 8988t C140(1.12)  LDD1253  [5]
 LDCM0375  CL106 PaTu 8988t C140(1.14)  LDD1254  [5]
 LDCM0376  CL107 PaTu 8988t C140(1.13)  LDD1255  [5]
 LDCM0377  CL108 PaTu 8988t C140(0.97)  LDD1256  [5]
 LDCM0378  CL109 PaTu 8988t C140(1.27)  LDD1257  [5]
 LDCM0379  CL11 HEK-293T C438(1.02)  LDD1583  [4]
 LDCM0380  CL110 PaTu 8988t C140(1.23)  LDD1259  [5]
 LDCM0381  CL111 PaTu 8988t C140(1.76)  LDD1260  [5]
 LDCM0383  CL113 HEK-293T C438(1.05)  LDD1587  [4]
 LDCM0385  CL115 HEK-293T C438(0.97)  LDD1589  [4]
 LDCM0387  CL117 HEK-293T C438(1.03)  LDD1591  [4]
 LDCM0389  CL119 HEK-293T C438(1.12)  LDD1593  [4]
 LDCM0390  CL12 HEK-293T C438(1.23)  LDD1594  [4]
 LDCM0392  CL121 HEK-293T C438(0.96)  LDD1596  [4]
 LDCM0394  CL123 HEK-293T C438(0.97)  LDD1598  [4]
 LDCM0396  CL125 HEK-293T C438(0.79)  LDD1600  [4]
 LDCM0398  CL127 HEK-293T C438(0.90)  LDD1602  [4]
 LDCM0400  CL13 HEK-293T C438(1.03)  LDD1604  [4]
 LDCM0402  CL15 HEK-293T C438(1.01)  LDD1606  [4]
 LDCM0403  CL16 PaTu 8988t C140(1.14)  LDD1282  [5]
 LDCM0404  CL17 PaTu 8988t C140(1.01)  LDD1283  [5]
 LDCM0405  CL18 PaTu 8988t C140(1.21)  LDD1284  [5]
 LDCM0406  CL19 PaTu 8988t C140(1.07)  LDD1285  [5]
 LDCM0408  CL20 PaTu 8988t C140(1.08)  LDD1287  [5]
 LDCM0409  CL21 PaTu 8988t C140(1.18)  LDD1288  [5]
 LDCM0410  CL22 PaTu 8988t C140(1.31)  LDD1289  [5]
 LDCM0411  CL23 PaTu 8988t C140(1.04)  LDD1290  [5]
 LDCM0412  CL24 PaTu 8988t C140(1.24)  LDD1291  [5]
 LDCM0413  CL25 PaTu 8988t C140(1.26)  LDD1292  [5]
 LDCM0414  CL26 PaTu 8988t C140(1.16)  LDD1293  [5]
 LDCM0415  CL27 PaTu 8988t C140(1.35)  LDD1294  [5]
 LDCM0416  CL28 PaTu 8988t C140(1.31)  LDD1295  [5]
 LDCM0417  CL29 PaTu 8988t C140(1.13)  LDD1296  [5]
 LDCM0418  CL3 HEK-293T C438(0.92)  LDD1622  [4]
 LDCM0419  CL30 PaTu 8988t C140(1.17)  LDD1298  [5]
 LDCM0420  CL31 PaTu 8988t C140(1.10)  LDD1299  [5]
 LDCM0421  CL32 PaTu 8988t C140(0.99)  LDD1300  [5]
 LDCM0422  CL33 PaTu 8988t C140(0.98)  LDD1301  [5]
 LDCM0423  CL34 PaTu 8988t C140(1.09)  LDD1302  [5]
 LDCM0424  CL35 PaTu 8988t C140(1.10)  LDD1303  [5]
 LDCM0425  CL36 PaTu 8988t C140(0.43)  LDD1304  [5]
 LDCM0426  CL37 PaTu 8988t C140(1.21)  LDD1305  [5]
 LDCM0428  CL39 PaTu 8988t C140(1.04)  LDD1307  [5]
 LDCM0430  CL40 PaTu 8988t C140(0.99)  LDD1309  [5]
 LDCM0431  CL41 PaTu 8988t C140(0.91)  LDD1310  [5]
 LDCM0432  CL42 PaTu 8988t C140(0.89)  LDD1311  [5]
 LDCM0433  CL43 PaTu 8988t C140(1.00)  LDD1312  [5]
 LDCM0434  CL44 PaTu 8988t C140(1.20)  LDD1313  [5]
 LDCM0435  CL45 PaTu 8988t C140(1.29)  LDD1314  [5]
 LDCM0436  CL46 HEK-293T C438(1.09)  LDD1640  [4]
 LDCM0437  CL47 HEK-293T C438(1.11)  LDD1641  [4]
 LDCM0438  CL48 HEK-293T C438(1.16)  LDD1642  [4]
 LDCM0439  CL49 HEK-293T C438(0.87)  LDD1643  [4]
 LDCM0440  CL5 HEK-293T C438(1.05)  LDD1644  [4]
 LDCM0444  CL53 HEK-293T C438(1.46)  LDD1647  [4]
 LDCM0445  CL54 HEK-293T C438(1.00)  LDD1648  [4]
 LDCM0447  CL56 HEK-293T C438(1.04)  LDD1650  [4]
 LDCM0448  CL57 HEK-293T C438(1.03)  LDD1651  [4]
 LDCM0449  CL58 HEK-293T C438(1.11)  LDD1652  [4]
 LDCM0450  CL59 HEK-293T C438(1.14)  LDD1653  [4]
 LDCM0451  CL6 HEK-293T C438(0.96)  LDD1654  [4]
 LDCM0452  CL60 HEK-293T C438(1.10)  LDD1655  [4]
 LDCM0453  CL61 HEK-293T C438(1.08)  LDD1656  [4]
 LDCM0455  CL63 HEK-293T C438(1.06)  LDD1658  [4]
 LDCM0457  CL65 HEK-293T C438(1.32)  LDD1660  [4]
 LDCM0458  CL66 HEK-293T C438(0.98)  LDD1661  [4]
 LDCM0460  CL68 HEK-293T C438(0.96)  LDD1663  [4]
 LDCM0461  CL69 HEK-293T C438(1.11)  LDD1664  [4]
 LDCM0463  CL70 HEK-293T C438(1.17)  LDD1666  [4]
 LDCM0464  CL71 HEK-293T C438(0.98)  LDD1667  [4]
 LDCM0465  CL72 HEK-293T C438(1.14)  LDD1668  [4]
 LDCM0466  CL73 HEK-293T C438(0.95)  LDD1669  [4]
 LDCM0469  CL76 PaTu 8988t C140(1.06)  LDD1348  [5]
 LDCM0470  CL77 PaTu 8988t C140(1.31)  LDD1349  [5]
 LDCM0471  CL78 PaTu 8988t C140(1.30)  LDD1350  [5]
 LDCM0472  CL79 PaTu 8988t C140(1.37)  LDD1351  [5]
 LDCM0473  CL8 HEK-293T C438(0.93)  LDD1676  [4]
 LDCM0474  CL80 PaTu 8988t C140(1.25)  LDD1353  [5]
 LDCM0475  CL81 PaTu 8988t C140(1.40)  LDD1354  [5]
 LDCM0476  CL82 PaTu 8988t C140(1.26)  LDD1355  [5]
 LDCM0477  CL83 PaTu 8988t C140(1.40)  LDD1356  [5]
 LDCM0478  CL84 PaTu 8988t C140(1.11)  LDD1357  [5]
 LDCM0479  CL85 PaTu 8988t C140(1.27)  LDD1358  [5]
 LDCM0480  CL86 PaTu 8988t C140(1.35)  LDD1359  [5]
 LDCM0481  CL87 PaTu 8988t C140(1.21)  LDD1360  [5]
 LDCM0482  CL88 PaTu 8988t C140(1.40)  LDD1361  [5]
 LDCM0483  CL89 PaTu 8988t C140(1.03)  LDD1362  [5]
 LDCM0484  CL9 HEK-293T C438(1.29)  LDD1687  [4]
 LDCM0485  CL90 PaTu 8988t C140(1.30)  LDD1364  [5]
 LDCM0487  CL92 HEK-293T C438(1.24)  LDD1690  [4]
 LDCM0488  CL93 HEK-293T C438(1.17)  LDD1691  [4]
 LDCM0489  CL94 HEK-293T C438(1.14)  LDD1692  [4]
 LDCM0490  CL95 HEK-293T C438(1.02)  LDD1693  [4]
 LDCM0491  CL96 HEK-293T C438(1.11)  LDD1694  [4]
 LDCM0492  CL97 HEK-293T C438(1.04)  LDD1695  [4]
 LDCM0494  CL99 HEK-293T C438(0.88)  LDD1697  [4]
 LDCM0495  E2913 HEK-293T C438(0.98)  LDD1698  [4]
 LDCM0468  Fragment33 HEK-293T C438(0.92)  LDD1671  [4]
 LDCM0427  Fragment51 PaTu 8988t C140(1.15)  LDD1306  [5]
 LDCM0022  KB02 HEK-293T C438(1.10); C140(1.06)  LDD1492  [4]
 LDCM0023  KB03 HEK-293T C438(0.62); C140(1.07)  LDD1497  [4]
 LDCM0024  KB05 MEWO C140(2.62)  LDD3319  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
5 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.