Details of the Target

General Information of Target

Target ID LDTP09103
Target Name UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 2 (B3GALNT2)
Gene Name B3GALNT2
Gene ID 148789
Synonyms
UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 2; Beta-1,3-GalNAc-T2; EC 2.4.1.313; Beta-1,3-N-acetylgalactosaminyltransferase II
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MRNWLVLLCPCVLGAALHLWLRLRSPPPACASGAGPADQLALFPQWKSTHYDVVVGVLSA
RNNHELRNVIRSTWMRHLLQHPTLSQRVLVKFIIGAHGCEVPVEDREDPYSCKLLNITNP
VLNQEIEAFSLSEDTSSGLPEDRVVSVSFRVLYPIVITSLGVFYDANDVGFQRNITVKLY
QAEQEEALFIARFSPPSCGVQVNKLWYKPVEQFILPESFEGTIVWESQDLHGLVSRNLHK
VTVNDGGGVLRVITAGEGALPHEFLEGVEGVAGGFIYTIQEGDALLHNLHSRPQRLIDHI
RNLHEEDALLKEESSIYDDIVFVDVVDTYRNVPAKLLNFYRWTVETTSFNLLLKTDDDCY
IDLEAVFNRIVQKNLDGPNFWWGNFRLNWAVDRTGKWQELEYPSPAYPAFACGSGYVISK
DIVKWLASNSGRLKTYQGEDVSMGIWMAAIGPKRYQDSLWLCEKTCETGMLSSPQYSPWE
LTELWKLKERCGDPCRCQAR
Target Bioclass
Enzyme
Family
Glycosyltransferase 31 family
Subcellular location
Golgi apparatus membrane
Function
Beta-1,3-N-acetylgalactosaminyltransferase that synthesizes a unique carbohydrate structure, GalNAc-beta-1-3GlcNAc, on N- and O-glycans. Has no galactose nor galactosaminyl transferase activity toward any acceptor substrate. Involved in alpha-dystroglycan (DAG1) glycosylation: acts coordinately with GTDC2/POMGnT2 to synthesize a GalNAc-beta3-GlcNAc-beta-terminus at the 4-position of protein O-mannose in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan, which is required for binding laminin G-like domain-containing extracellular proteins with high affinity.
Uniprot ID
Q8NCR0
Ensemble ID
ENST00000313984.3
HGNC ID
HGNC:28596

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
J82 SNV: p.D327G .
JM1 SNV: p.T49S .
MCC13 Substitution: p.G221N .
MEWO SNV: p.E345G; p.S348T .
SNGM SNV: p.L42S .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C239(1.86)  LDD3383  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C198(0.88)  LDD1508  [2]
 LDCM0226  AC11 HEK-293T C30(0.92)  LDD1509  [2]
 LDCM0237  AC12 HEK-293T C198(0.97)  LDD1510  [2]
 LDCM0259  AC14 HEK-293T C198(1.09)  LDD1512  [2]
 LDCM0270  AC15 HEK-293T C30(0.95); C198(0.93)  LDD1513  [2]
 LDCM0277  AC18 HEK-293T C198(0.85)  LDD1516  [2]
 LDCM0278  AC19 HEK-293T C30(1.04)  LDD1517  [2]
 LDCM0279  AC2 HEK-293T C198(1.12)  LDD1518  [2]
 LDCM0280  AC20 HEK-293T C198(0.96)  LDD1519  [2]
 LDCM0281  AC21 HEK-293T C198(1.08)  LDD1520  [2]
 LDCM0282  AC22 HEK-293T C198(0.88)  LDD1521  [2]
 LDCM0283  AC23 HEK-293T C30(0.92); C198(0.97)  LDD1522  [2]
 LDCM0284  AC24 HEK-293T C198(0.93)  LDD1523  [2]
 LDCM0286  AC26 HEK-293T C198(0.87)  LDD1525  [2]
 LDCM0287  AC27 HEK-293T C30(0.88)  LDD1526  [2]
 LDCM0288  AC28 HEK-293T C198(0.84)  LDD1527  [2]
 LDCM0289  AC29 HEK-293T C198(0.98)  LDD1528  [2]
 LDCM0290  AC3 HEK-293T C30(1.06)  LDD1529  [2]
 LDCM0291  AC30 HEK-293T C198(1.01)  LDD1530  [2]
 LDCM0292  AC31 HEK-293T C30(1.10); C198(1.02)  LDD1531  [2]
 LDCM0293  AC32 HEK-293T C198(0.99)  LDD1532  [2]
 LDCM0295  AC34 HEK-293T C198(0.86)  LDD1534  [2]
 LDCM0296  AC35 HEK-293T C30(0.91)  LDD1535  [2]
 LDCM0297  AC36 HEK-293T C198(0.95)  LDD1536  [2]
 LDCM0298  AC37 HEK-293T C198(0.97)  LDD1537  [2]
 LDCM0299  AC38 HEK-293T C198(1.01)  LDD1538  [2]
 LDCM0300  AC39 HEK-293T C30(1.02); C198(1.03)  LDD1539  [2]
 LDCM0301  AC4 HEK-293T C198(0.86)  LDD1540  [2]
 LDCM0302  AC40 HEK-293T C198(1.18)  LDD1541  [2]
 LDCM0304  AC42 HEK-293T C198(1.04)  LDD1543  [2]
 LDCM0305  AC43 HEK-293T C30(1.00)  LDD1544  [2]
 LDCM0306  AC44 HEK-293T C198(0.87)  LDD1545  [2]
 LDCM0307  AC45 HEK-293T C198(0.95)  LDD1546  [2]
 LDCM0308  AC46 HEK-293T C198(0.93)  LDD1547  [2]
 LDCM0309  AC47 HEK-293T C30(0.96); C198(0.90)  LDD1548  [2]
 LDCM0310  AC48 HEK-293T C198(1.06)  LDD1549  [2]
 LDCM0312  AC5 HEK-293T C198(0.96)  LDD1551  [2]
 LDCM0313  AC50 HEK-293T C198(0.99)  LDD1552  [2]
 LDCM0314  AC51 HEK-293T C30(0.92)  LDD1553  [2]
 LDCM0315  AC52 HEK-293T C198(0.90)  LDD1554  [2]
 LDCM0316  AC53 HEK-293T C198(0.96)  LDD1555  [2]
 LDCM0317  AC54 HEK-293T C198(1.04)  LDD1556  [2]
 LDCM0318  AC55 HEK-293T C30(0.97); C198(0.98)  LDD1557  [2]
 LDCM0319  AC56 HEK-293T C198(0.97)  LDD1558  [2]
 LDCM0321  AC58 HEK-293T C198(0.90)  LDD1560  [2]
 LDCM0322  AC59 HEK-293T C30(0.97)  LDD1561  [2]
 LDCM0323  AC6 HEK-293T C198(1.07)  LDD1562  [2]
 LDCM0324  AC60 HEK-293T C198(0.94)  LDD1563  [2]
 LDCM0325  AC61 HEK-293T C198(0.99)  LDD1564  [2]
 LDCM0326  AC62 HEK-293T C198(1.08)  LDD1565  [2]
 LDCM0327  AC63 HEK-293T C30(1.17); C198(1.01)  LDD1566  [2]
 LDCM0328  AC64 HEK-293T C198(1.13)  LDD1567  [2]
 LDCM0334  AC7 HEK-293T C30(1.01); C198(0.97)  LDD1568  [2]
 LDCM0345  AC8 HEK-293T C198(0.94)  LDD1569  [2]
 LDCM0248  AKOS034007472 HEK-293T C198(1.05)  LDD1511  [2]
 LDCM0275  AKOS034007705 HEK-293T C198(1.14)  LDD1514  [2]
 LDCM0367  CL1 HEK-293T C30(1.07); C198(1.13)  LDD1571  [2]
 LDCM0368  CL10 HEK-293T C198(0.99)  LDD1572  [2]
 LDCM0370  CL101 HEK-293T C30(1.13); C198(1.09)  LDD1574  [2]
 LDCM0371  CL102 HEK-293T C198(1.03)  LDD1575  [2]
 LDCM0372  CL103 HEK-293T C30(0.82); C198(0.97)  LDD1576  [2]
 LDCM0374  CL105 HEK-293T C30(1.15); C198(0.92)  LDD1578  [2]
 LDCM0375  CL106 HEK-293T C198(1.10)  LDD1579  [2]
 LDCM0376  CL107 HEK-293T C30(0.94); C198(0.98)  LDD1580  [2]
 LDCM0378  CL109 HEK-293T C30(1.06); C198(1.03)  LDD1582  [2]
 LDCM0379  CL11 HEK-293T C30(1.01); C198(1.02)  LDD1583  [2]
 LDCM0380  CL110 HEK-293T C198(1.01)  LDD1584  [2]
 LDCM0381  CL111 HEK-293T C30(1.04); C198(1.11)  LDD1585  [2]
 LDCM0383  CL113 HEK-293T C30(1.13); C198(0.96)  LDD1587  [2]
 LDCM0384  CL114 HEK-293T C198(1.06)  LDD1588  [2]
 LDCM0385  CL115 HEK-293T C30(1.11); C198(1.10)  LDD1589  [2]
 LDCM0387  CL117 HEK-293T C30(0.92); C198(0.94)  LDD1591  [2]
 LDCM0388  CL118 HEK-293T C198(1.03)  LDD1592  [2]
 LDCM0389  CL119 HEK-293T C30(1.01); C198(1.04)  LDD1593  [2]
 LDCM0390  CL12 HEK-293T C198(1.47)  LDD1594  [2]
 LDCM0392  CL121 HEK-293T C30(1.03); C198(1.04)  LDD1596  [2]
 LDCM0393  CL122 HEK-293T C198(1.01)  LDD1597  [2]
 LDCM0394  CL123 HEK-293T C30(1.00); C198(1.02)  LDD1598  [2]
 LDCM0396  CL125 HEK-293T C30(1.06); C198(1.05)  LDD1600  [2]
 LDCM0397  CL126 HEK-293T C198(1.00)  LDD1601  [2]
 LDCM0398  CL127 HEK-293T C30(1.14); C198(0.90)  LDD1602  [2]
 LDCM0400  CL13 HEK-293T C30(1.28); C198(0.97)  LDD1604  [2]
 LDCM0401  CL14 HEK-293T C198(1.13)  LDD1605  [2]
 LDCM0402  CL15 HEK-293T C30(1.04); C198(0.94)  LDD1606  [2]
 LDCM0405  CL18 HEK-293T C198(1.04)  LDD1609  [2]
 LDCM0406  CL19 HEK-293T C30(0.87)  LDD1610  [2]
 LDCM0407  CL2 HEK-293T C198(1.18)  LDD1611  [2]
 LDCM0408  CL20 HEK-293T C198(1.08)  LDD1612  [2]
 LDCM0409  CL21 HEK-293T C198(1.06)  LDD1613  [2]
 LDCM0410  CL22 HEK-293T C198(0.88)  LDD1614  [2]
 LDCM0411  CL23 HEK-293T C30(0.90); C198(1.03)  LDD1615  [2]
 LDCM0412  CL24 HEK-293T C198(1.30)  LDD1616  [2]
 LDCM0413  CL25 HEK-293T C30(0.86); C198(0.97)  LDD1617  [2]
 LDCM0414  CL26 HEK-293T C198(1.03)  LDD1618  [2]
 LDCM0415  CL27 HEK-293T C30(0.96); C198(1.06)  LDD1619  [2]
 LDCM0418  CL3 HEK-293T C30(1.29); C198(1.10)  LDD1622  [2]
 LDCM0419  CL30 HEK-293T C198(1.14)  LDD1623  [2]
 LDCM0420  CL31 HEK-293T C30(0.85)  LDD1624  [2]
 LDCM0421  CL32 HEK-293T C198(1.03)  LDD1625  [2]
 LDCM0422  CL33 HEK-293T C198(1.08)  LDD1626  [2]
 LDCM0423  CL34 HEK-293T C198(1.24)  LDD1627  [2]
 LDCM0424  CL35 HEK-293T C30(0.93); C198(0.97)  LDD1628  [2]
 LDCM0425  CL36 HEK-293T C198(1.13)  LDD1629  [2]
 LDCM0426  CL37 HEK-293T C30(0.95); C198(0.91)  LDD1630  [2]
 LDCM0428  CL39 HEK-293T C30(0.89); C198(1.01)  LDD1632  [2]
 LDCM0432  CL42 HEK-293T C198(0.91)  LDD1636  [2]
 LDCM0433  CL43 HEK-293T C30(0.87)  LDD1637  [2]
 LDCM0434  CL44 HEK-293T C198(0.97)  LDD1638  [2]
 LDCM0435  CL45 HEK-293T C198(1.03)  LDD1639  [2]
 LDCM0436  CL46 HEK-293T C198(1.11)  LDD1640  [2]
 LDCM0437  CL47 HEK-293T C30(1.01); C198(1.05)  LDD1641  [2]
 LDCM0438  CL48 HEK-293T C198(1.12)  LDD1642  [2]
 LDCM0439  CL49 HEK-293T C30(1.15); C198(0.96)  LDD1643  [2]
 LDCM0441  CL50 HEK-293T C198(1.08)  LDD1645  [2]
 LDCM0445  CL54 HEK-293T C198(0.94)  LDD1648  [2]
 LDCM0446  CL55 HEK-293T C30(0.84)  LDD1649  [2]
 LDCM0447  CL56 HEK-293T C198(1.20)  LDD1650  [2]
 LDCM0448  CL57 HEK-293T C198(1.02)  LDD1651  [2]
 LDCM0449  CL58 HEK-293T C198(1.29)  LDD1652  [2]
 LDCM0450  CL59 HEK-293T C30(1.18); C198(1.07)  LDD1653  [2]
 LDCM0451  CL6 HEK-293T C198(1.11)  LDD1654  [2]
 LDCM0452  CL60 HEK-293T C198(1.06)  LDD1655  [2]
 LDCM0453  CL61 HEK-293T C30(0.97); C198(1.10)  LDD1656  [2]
 LDCM0454  CL62 HEK-293T C198(1.11)  LDD1657  [2]
 LDCM0455  CL63 HEK-293T C30(1.08); C198(1.22)  LDD1658  [2]
 LDCM0458  CL66 HEK-293T C198(1.16)  LDD1661  [2]
 LDCM0459  CL67 HEK-293T C30(0.90)  LDD1662  [2]
 LDCM0460  CL68 HEK-293T C198(0.99)  LDD1663  [2]
 LDCM0461  CL69 HEK-293T C198(1.00)  LDD1664  [2]
 LDCM0462  CL7 HEK-293T C30(1.00)  LDD1665  [2]
 LDCM0463  CL70 HEK-293T C198(1.18)  LDD1666  [2]
 LDCM0464  CL71 HEK-293T C30(1.03); C198(0.97)  LDD1667  [2]
 LDCM0465  CL72 HEK-293T C198(1.05)  LDD1668  [2]
 LDCM0466  CL73 HEK-293T C30(1.01); C198(1.16)  LDD1669  [2]
 LDCM0467  CL74 HEK-293T C198(1.16)  LDD1670  [2]
 LDCM0471  CL78 HEK-293T C198(1.06)  LDD1674  [2]
 LDCM0472  CL79 HEK-293T C30(0.91)  LDD1675  [2]
 LDCM0473  CL8 HEK-293T C198(0.95)  LDD1676  [2]
 LDCM0474  CL80 HEK-293T C198(0.98)  LDD1677  [2]
 LDCM0475  CL81 HEK-293T C198(1.02)  LDD1678  [2]
 LDCM0476  CL82 HEK-293T C198(1.19)  LDD1679  [2]
 LDCM0477  CL83 HEK-293T C30(0.99); C198(0.99)  LDD1680  [2]
 LDCM0478  CL84 HEK-293T C198(1.34)  LDD1681  [2]
 LDCM0479  CL85 HEK-293T C30(1.11); C198(1.00)  LDD1682  [2]
 LDCM0480  CL86 HEK-293T C198(1.01)  LDD1683  [2]
 LDCM0481  CL87 HEK-293T C30(0.60); C198(0.97)  LDD1684  [2]
 LDCM0484  CL9 HEK-293T C198(1.09)  LDD1687  [2]
 LDCM0485  CL90 HEK-293T C198(0.99)  LDD1688  [2]
 LDCM0486  CL91 HEK-293T C30(1.09)  LDD1689  [2]
 LDCM0487  CL92 HEK-293T C198(0.99)  LDD1690  [2]
 LDCM0488  CL93 HEK-293T C198(1.00)  LDD1691  [2]
 LDCM0489  CL94 HEK-293T C198(1.04)  LDD1692  [2]
 LDCM0490  CL95 HEK-293T C30(1.21); C198(1.01)  LDD1693  [2]
 LDCM0491  CL96 HEK-293T C198(1.10)  LDD1694  [2]
 LDCM0492  CL97 HEK-293T C30(1.06); C198(0.98)  LDD1695  [2]
 LDCM0493  CL98 HEK-293T C198(1.17)  LDD1696  [2]
 LDCM0494  CL99 HEK-293T C30(0.82); C198(0.98)  LDD1697  [2]
 LDCM0495  E2913 HEK-293T C30(1.06); C198(1.08)  LDD1698  [2]
 LDCM0468  Fragment33 HEK-293T C30(0.99); C198(1.07)  LDD1671  [2]
 LDCM0427  Fragment51 HEK-293T C198(1.18)  LDD1631  [2]
 LDCM0022  KB02 A2780 C239(1.47)  LDD2254  [1]
 LDCM0023  KB03 Caov-3 C239(1.75)  LDD2712  [1]
 LDCM0024  KB05 OVCAR-5 C239(1.86)  LDD3383  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402