Details of the Target

General Information of Target

Target ID LDTP08734
Target Name Citramalyl-CoA lyase, mitochondrial (CLYBL)
Gene Name CLYBL
Gene ID 171425
Synonyms
CLB; Citramalyl-CoA lyase, mitochondrial; EC 4.1.3.25; (3S)-malyl-CoA thioesterase; EC 3.1.2.30; Beta-methylmalate synthase; EC 2.3.3.-; Citrate lyase subunit beta-like protein; Citrate lyase beta-like; Malate synthase; EC 2.3.3.9
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MALRLLRRAARGAAAAALLRLKASLAADIPRLGYSSSSHHKYIPRRAVLYVPGNDEKKIK
KIPSLNVDCAVLDCEDGVAANKKNEARLRIVKTLEDIDLGPTEKCVRVNSVSSGLAEEDL
ETLLQSRVLPSSLMLPKVESPEEIQWFADKFSFHLKGRKLEQPMNLIPFVETAMGLLNFK
AVCEETLKVGPQVGLFLDAVVFGGEDFRASIGATSSKETLDILYARQKIVVIAKAFGLQA
IDLVYIDFRDGAGLLRQSREGAAMGFTGKQVIHPNQIAVVQEQFSPSPEKIKWAEELIAA
FKEHQQLGKGAFTFQGSMIDMPLLKQAQNTVTLATSIKEK
Target Bioclass
Enzyme
Family
HpcH/HpaI aldolase family, Citrate lyase beta subunit-like subfamily
Subcellular location
Mitochondrion
Function
Mitochondrial citramalyl-CoA lyase indirectly involved in the vitamin B12 metabolism. Converts citramalyl-CoA into acetyl-CoA and pyruvate in the C5-dicarboxylate catabolism pathway. The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, a vitamin B12-poisoning metabolite. Also acts as a malate synthase in vitro, converting glyoxylate and acetyl-CoA to malate. Also displays malyl-CoA thioesterase activity. Also acts as a beta-methylmalate synthase in vitro, by mediating conversion of glyoxylate and propionyl-CoA to beta-methylmalate. Also has very weak citramalate synthase activity in vitro.
Uniprot ID
Q8N0X4
Ensemble ID
ENST00000339105.9
HGNC ID
HGNC:18355

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
Ishikawa (Heraklio) 02 ER SNV: p.D76A .
KYSE180 SNV: p.E185K .
MEWO SNV: p.P44S .
TE10 SNV: p.S112F .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		N.A.  LDD0241  [1]
DBIA
 Probe Info  		C183(2.59)  LDD3386  [2]
Acrolein
 Probe Info  		N.A.  LDD0231  [3]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [4]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0237  AC12 HEK-293T C183(1.00)  LDD1510  [5]
 LDCM0259  AC14 HEK-293T C183(1.03)  LDD1512  [5]
 LDCM0270  AC15 HEK-293T C183(1.33)  LDD1513  [5]
 LDCM0280  AC20 HEK-293T C183(0.91)  LDD1519  [5]
 LDCM0281  AC21 HEK-293T C183(1.02)  LDD1520  [5]
 LDCM0282  AC22 HEK-293T C183(0.90)  LDD1521  [5]
 LDCM0283  AC23 HEK-293T C183(0.99)  LDD1522  [5]
 LDCM0284  AC24 HEK-293T C183(0.98)  LDD1523  [5]
 LDCM0288  AC28 HEK-293T C183(0.93)  LDD1527  [5]
 LDCM0289  AC29 HEK-293T C183(0.96)  LDD1528  [5]
 LDCM0291  AC30 HEK-293T C183(1.00)  LDD1530  [5]
 LDCM0292  AC31 HEK-293T C183(0.99)  LDD1531  [5]
 LDCM0293  AC32 HEK-293T C183(0.92)  LDD1532  [5]
 LDCM0297  AC36 HEK-293T C183(0.95)  LDD1536  [5]
 LDCM0298  AC37 HEK-293T C183(1.10)  LDD1537  [5]
 LDCM0299  AC38 HEK-293T C183(1.12)  LDD1538  [5]
 LDCM0300  AC39 HEK-293T C183(0.89)  LDD1539  [5]
 LDCM0301  AC4 HEK-293T C183(1.08)  LDD1540  [5]
 LDCM0302  AC40 HEK-293T C183(1.14)  LDD1541  [5]
 LDCM0306  AC44 HEK-293T C183(1.21)  LDD1545  [5]
 LDCM0307  AC45 HEK-293T C183(1.10)  LDD1546  [5]
 LDCM0308  AC46 HEK-293T C183(1.11)  LDD1547  [5]
 LDCM0309  AC47 HEK-293T C183(0.89)  LDD1548  [5]
 LDCM0310  AC48 HEK-293T C183(0.99)  LDD1549  [5]
 LDCM0312  AC5 HEK-293T C183(0.96)  LDD1551  [5]
 LDCM0315  AC52 HEK-293T C183(0.94)  LDD1554  [5]
 LDCM0316  AC53 HEK-293T C183(1.01)  LDD1555  [5]
 LDCM0317  AC54 HEK-293T C183(1.04)  LDD1556  [5]
 LDCM0318  AC55 HEK-293T C183(0.98)  LDD1557  [5]
 LDCM0319  AC56 HEK-293T C183(1.03)  LDD1558  [5]
 LDCM0323  AC6 HEK-293T C183(1.05)  LDD1562  [5]
 LDCM0324  AC60 HEK-293T C183(0.95)  LDD1563  [5]
 LDCM0325  AC61 HEK-293T C183(1.02)  LDD1564  [5]
 LDCM0326  AC62 HEK-293T C183(1.03)  LDD1565  [5]
 LDCM0327  AC63 HEK-293T C183(0.97)  LDD1566  [5]
 LDCM0328  AC64 HEK-293T C183(0.90)  LDD1567  [5]
 LDCM0334  AC7 HEK-293T C183(0.90)  LDD1568  [5]
 LDCM0345  AC8 HEK-293T C183(0.87)  LDD1569  [5]
 LDCM0248  AKOS034007472 HEK-293T C183(1.02)  LDD1511  [5]
 LDCM0275  AKOS034007705 HEK-293T C183(0.98)  LDD1514  [5]
 LDCM0367  CL1 HEK-293T C183(1.01)  LDD1571  [5]
 LDCM0368  CL10 HEK-293T C183(1.32)  LDD1572  [5]
 LDCM0370  CL101 HEK-293T C183(0.95)  LDD1574  [5]
 LDCM0371  CL102 HEK-293T C183(0.94)  LDD1575  [5]
 LDCM0372  CL103 HEK-293T C183(1.04)  LDD1576  [5]
 LDCM0374  CL105 HEK-293T C183(0.99)  LDD1578  [5]
 LDCM0375  CL106 HEK-293T C183(0.99)  LDD1579  [5]
 LDCM0376  CL107 HEK-293T C183(0.93)  LDD1580  [5]
 LDCM0378  CL109 HEK-293T C183(1.01)  LDD1582  [5]
 LDCM0379  CL11 HEK-293T C183(1.27)  LDD1583  [5]
 LDCM0380  CL110 HEK-293T C183(0.99)  LDD1584  [5]
 LDCM0381  CL111 HEK-293T C183(0.93)  LDD1585  [5]
 LDCM0383  CL113 HEK-293T C183(1.08)  LDD1587  [5]
 LDCM0384  CL114 HEK-293T C183(0.96)  LDD1588  [5]
 LDCM0385  CL115 HEK-293T C183(1.10)  LDD1589  [5]
 LDCM0387  CL117 HEK-293T C183(1.02)  LDD1591  [5]
 LDCM0388  CL118 HEK-293T C183(1.01)  LDD1592  [5]
 LDCM0389  CL119 HEK-293T C183(1.14)  LDD1593  [5]
 LDCM0390  CL12 HEK-293T C183(1.24)  LDD1594  [5]
 LDCM0392  CL121 HEK-293T C183(1.01)  LDD1596  [5]
 LDCM0393  CL122 HEK-293T C183(1.05)  LDD1597  [5]
 LDCM0394  CL123 HEK-293T C183(1.07)  LDD1598  [5]
 LDCM0396  CL125 HEK-293T C183(0.94)  LDD1600  [5]
 LDCM0397  CL126 HEK-293T C183(0.99)  LDD1601  [5]
 LDCM0398  CL127 HEK-293T C183(0.90)  LDD1602  [5]
 LDCM0400  CL13 HEK-293T C183(1.02)  LDD1604  [5]
 LDCM0401  CL14 HEK-293T C183(1.09)  LDD1605  [5]
 LDCM0402  CL15 HEK-293T C183(1.13)  LDD1606  [5]
 LDCM0407  CL2 HEK-293T C183(1.13)  LDD1611  [5]
 LDCM0408  CL20 HEK-293T C183(1.26)  LDD1612  [5]
 LDCM0409  CL21 HEK-293T C183(1.11)  LDD1613  [5]
 LDCM0410  CL22 HEK-293T C183(1.29)  LDD1614  [5]
 LDCM0411  CL23 HEK-293T C183(1.37)  LDD1615  [5]
 LDCM0412  CL24 HEK-293T C183(1.31)  LDD1616  [5]
 LDCM0413  CL25 HEK-293T C183(0.96)  LDD1617  [5]
 LDCM0414  CL26 HEK-293T C183(1.04)  LDD1618  [5]
 LDCM0415  CL27 HEK-293T C183(1.05)  LDD1619  [5]
 LDCM0418  CL3 HEK-293T C183(1.23)  LDD1622  [5]
 LDCM0421  CL32 HEK-293T C183(1.04)  LDD1625  [5]
 LDCM0422  CL33 HEK-293T C183(1.36)  LDD1626  [5]
 LDCM0423  CL34 HEK-293T C183(1.32)  LDD1627  [5]
 LDCM0424  CL35 HEK-293T C183(1.32)  LDD1628  [5]
 LDCM0425  CL36 HEK-293T C183(1.20)  LDD1629  [5]
 LDCM0426  CL37 HEK-293T C183(0.95)  LDD1630  [5]
 LDCM0428  CL39 HEK-293T C183(1.07)  LDD1632  [5]
 LDCM0434  CL44 HEK-293T C183(1.02)  LDD1638  [5]
 LDCM0435  CL45 HEK-293T C183(1.21)  LDD1639  [5]
 LDCM0436  CL46 HEK-293T C183(1.26)  LDD1640  [5]
 LDCM0437  CL47 HEK-293T C183(1.28)  LDD1641  [5]
 LDCM0438  CL48 HEK-293T C183(1.33)  LDD1642  [5]
 LDCM0439  CL49 HEK-293T C183(1.13)  LDD1643  [5]
 LDCM0441  CL50 HEK-293T C183(1.08)  LDD1645  [5]
 LDCM0447  CL56 HEK-293T C183(1.20)  LDD1650  [5]
 LDCM0448  CL57 HEK-293T C183(1.24)  LDD1651  [5]
 LDCM0449  CL58 HEK-293T C183(1.33)  LDD1652  [5]
 LDCM0450  CL59 HEK-293T C183(1.31)  LDD1653  [5]
 LDCM0452  CL60 HEK-293T C183(1.32)  LDD1655  [5]
 LDCM0453  CL61 HEK-293T C183(1.00)  LDD1656  [5]
 LDCM0454  CL62 HEK-293T C183(0.98)  LDD1657  [5]
 LDCM0455  CL63 HEK-293T C183(1.17)  LDD1658  [5]
 LDCM0460  CL68 HEK-293T C183(1.28)  LDD1663  [5]
 LDCM0461  CL69 HEK-293T C183(1.23)  LDD1664  [5]
 LDCM0463  CL70 HEK-293T C183(1.37)  LDD1666  [5]
 LDCM0464  CL71 HEK-293T C183(1.19)  LDD1667  [5]
 LDCM0465  CL72 HEK-293T C183(1.24)  LDD1668  [5]
 LDCM0466  CL73 HEK-293T C183(0.95)  LDD1669  [5]
 LDCM0467  CL74 HEK-293T C183(1.07)  LDD1670  [5]
 LDCM0473  CL8 HEK-293T C183(1.43)  LDD1676  [5]
 LDCM0474  CL80 HEK-293T C183(1.08)  LDD1677  [5]
 LDCM0475  CL81 HEK-293T C183(1.18)  LDD1678  [5]
 LDCM0476  CL82 HEK-293T C183(1.17)  LDD1679  [5]
 LDCM0477  CL83 HEK-293T C183(1.36)  LDD1680  [5]
 LDCM0478  CL84 HEK-293T C183(1.23)  LDD1681  [5]
 LDCM0479  CL85 HEK-293T C183(1.01)  LDD1682  [5]
 LDCM0480  CL86 HEK-293T C183(0.96)  LDD1683  [5]
 LDCM0481  CL87 HEK-293T C183(1.07)  LDD1684  [5]
 LDCM0484  CL9 HEK-293T C183(1.23)  LDD1687  [5]
 LDCM0487  CL92 HEK-293T C183(1.16)  LDD1690  [5]
 LDCM0488  CL93 HEK-293T C183(1.10)  LDD1691  [5]
 LDCM0489  CL94 HEK-293T C183(1.25)  LDD1692  [5]
 LDCM0490  CL95 HEK-293T C183(1.14)  LDD1693  [5]
 LDCM0491  CL96 HEK-293T C183(1.10)  LDD1694  [5]
 LDCM0492  CL97 HEK-293T C183(0.95)  LDD1695  [5]
 LDCM0493  CL98 HEK-293T C183(0.96)  LDD1696  [5]
 LDCM0494  CL99 HEK-293T C183(0.95)  LDD1697  [5]
 LDCM0495  E2913 HEK-293T C183(1.09)  LDD1698  [5]
 LDCM0468  Fragment33 HEK-293T C183(1.17)  LDD1671  [5]
 LDCM0427  Fragment51 HEK-293T C183(1.08)  LDD1631  [5]
 LDCM0022  KB02 HEK-293T C183(1.18)  LDD1492  [5]
 LDCM0023  KB03 HEK-293T C183(0.98)  LDD1497  [5]
 LDCM0024  KB05 OVK-18 C183(2.59)  LDD3386  [2]
 LDCM0109  NEM HeLa N.A.  LDD0231  [3]

References

1 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
4 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402