Details of the Target

General Information of Target

Target ID LDTP08447
Target Name Polypeptide N-acetylgalactosaminyltransferase 13 (GALNT13)
Gene Name GALNT13
Gene ID 114805
Synonyms
KIAA1918; Polypeptide N-acetylgalactosaminyltransferase 13; EC 2.4.1.41; Polypeptide GalNAc transferase 13; GalNAc-T13; pp-GaNTase 13; Protein-UDP acetylgalactosaminyltransferase 13; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 13
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MRRFVYCKVVLATSLMWVLVDVFLLLYFSECNKCDDKKERSLLPALRAVISRNQEGPGEM
GKAVLIPKDDQEKMKELFKINQFNLMASDLIALNRSLPDVRLEGCKTKVYPDELPNTSVV
IVFHNEAWSTLLRTVYSVINRSPHYLLSEVILVDDASERDFLKLTLENYVKNLEVPVKII
RMEERSGLIRARLRGAAASKGQVITFLDAHCECTLGWLEPLLARIKEDRKTVVCPIIDVI
SDDTFEYMAGSDMTYGGFNWKLNFRWYPVPQREMDRRKGDRTLPVRTPTMAGGLFSIDRN
YFEEIGTYDAGMDIWGGENLEMSFRIWQCGGSLEIVTCSHVGHVFRKATPYTFPGGTGHV
INKNNRRLAEVWMDEFKDFFYIISPGVVKVDYGDVSVRKTLRENLKCKPFSWYLENIYPD
SQIPRRYYSLGEIRNVETNQCLDNMGRKENEKVGIFNCHGMGGNQVFSYTADKEIRTDDL
CLDVSRLNGPVIMLKCHHMRGNQLWEYDAERLTLRHVNSNQCLDEPSEEDKMVPTMQDCS
GSRSQQWLLRNMTLGT
Target Bioclass
Enzyme
Family
Glycosyltransferase 2 family, GalNAc-T subfamily
Subcellular location
Golgi apparatus membrane
Function
Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine (GalNAc) residue from UDP-GalNAc to a serine or threonine residue on the protein receptor. Generates GalNAc-O-Ser/Thr structure also known as Tn antigen, which itself is immunogenic but also serves as a precursor for the synthesis of different mucin-type O-glycan core structures. Contributes to the synthesis of O-linked glycans on mucins and proteoglycans of the central nervous system. May promote neurogenesis through glycosylation and stabilization of PDPN.; [Isoform 1]: Can glycosylate both unmodified peptides and glycopeptides that already contain an O-linked GalNAc sugar. Transfers GalNAc to Thr-/Ser-rich tandem repeats GTTPSPVPTTSTTSAP of MUC5AC, specifically on Thr-3 of non-glycosylated MUC5AC peptide, on Thr-12 and Thr-13 of preglycosylated MUC5AC at Thr-3 (MUC5AC-3), on Thr-3 of preglycosylated MUC5AC at Thr-13 (MUC5AC-13) and on Thr-12 of preglycosylated MUC5AC at Thr-3 and Thr-13 (MUC5AC-3,13). Transfers GalNAc to three consecutive serine/threonine residues on SDC3 forming a triplet-Tn epitope expressed in Purkinje cells of the developing brain.; [Isoform 3]: Can glycosylate both unmodified peptides and glycopeptides that already contain an O-linked GalNAc sugar. Transfers GalNAc to Thr-/Ser-rich tandem repeats GTTPSPVPTTSTTSAP of MUC5AC, specifically on Thr-3 of non-glycosylated MUC5AC peptide, on Thr-12 and Thr-13 of preglycosylated MUC5AC at Thr-3 (MUC5AC-3), on Thr-3 of preglycosylated MUC5AC at Thr-13 (MUC5AC-13) and on Thr-12 of preglycosylated MUC5AC at Thr-3 and Thr-13 (MUC5AC-3,13).
Uniprot ID
Q8IUC8
Ensemble ID
ENST00000392825.8
HGNC ID
HGNC:23242
ChEMBL ID
CHEMBL4523392

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AGS SNV: p.R367G .
CAL51 SNV: p.T214K .
CAOV3 SNV: p.W547C .
DU145 SNV: p.I382V .
HCT15 SNV: p.S411Y .
HS936T SNV: p.E76K; p.G306E .
HT1080 SNV: p.L114I .
HT115 SNV: p.K200N .
IPC298 SNV: p.E449K .
KMCH1 SNV: p.H516R .
KMS12BM SNV: p.R101S .
LNCaP clone FGC SNV: p.I383V .
MEWO SNV: p.E212G; p.P385T; p.E451K; p.Q465Ter .
NCIH1993 SNV: p.K363N .
NCIH2170 SNV: p.E149A .
NCIH2286 SNV: p.R47S .
NCIH2291 Deletion: p.V533CfsTer17 .
NCIH661 SNV: p.S384Y .
RPMI8226 Substitution: p.W17_V18delinsCF .
SKMM2 SNV: p.Y169N .
SNGM Deletion: p.N172IfsTer2 .
SW1271 SNV: p.N364K .
UACC257 SNV: p.E59K .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FBPP2
 Probe Info  		10.58  LDD0318  [1]
DBIA
 Probe Info  		C407(1.11)  LDD1507  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C407(1.11)  LDD1507  [2]
 LDCM0215  AC10 HEK-293T C407(1.30)  LDD1508  [2]
 LDCM0226  AC11 HEK-293T C407(0.90)  LDD1509  [2]
 LDCM0237  AC12 HEK-293T C407(1.13)  LDD1510  [2]
 LDCM0259  AC14 HEK-293T C407(0.85)  LDD1512  [2]
 LDCM0270  AC15 HEK-293T C407(1.02)  LDD1513  [2]
 LDCM0276  AC17 HEK-293T C407(0.99)  LDD1515  [2]
 LDCM0277  AC18 HEK-293T C407(1.26)  LDD1516  [2]
 LDCM0278  AC19 HEK-293T C407(1.59)  LDD1517  [2]
 LDCM0279  AC2 HEK-293T C407(1.10)  LDD1518  [2]
 LDCM0280  AC20 HEK-293T C407(0.99)  LDD1519  [2]
 LDCM0282  AC22 HEK-293T C407(0.97)  LDD1521  [2]
 LDCM0283  AC23 HEK-293T C407(1.28)  LDD1522  [2]
 LDCM0285  AC25 HEK-293T C407(1.05)  LDD1524  [2]
 LDCM0286  AC26 HEK-293T C407(1.26)  LDD1525  [2]
 LDCM0287  AC27 HEK-293T C407(0.77)  LDD1526  [2]
 LDCM0288  AC28 HEK-293T C407(1.06)  LDD1527  [2]
 LDCM0290  AC3 HEK-293T C407(1.45)  LDD1529  [2]
 LDCM0291  AC30 HEK-293T C407(1.11)  LDD1530  [2]
 LDCM0292  AC31 HEK-293T C407(0.96)  LDD1531  [2]
 LDCM0294  AC33 HEK-293T C407(1.17)  LDD1533  [2]
 LDCM0295  AC34 HEK-293T C407(1.16)  LDD1534  [2]
 LDCM0296  AC35 HEK-293T C407(1.00)  LDD1535  [2]
 LDCM0297  AC36 HEK-293T C407(1.16)  LDD1536  [2]
 LDCM0299  AC38 HEK-293T C407(1.08)  LDD1538  [2]
 LDCM0300  AC39 HEK-293T C407(1.08)  LDD1539  [2]
 LDCM0301  AC4 HEK-293T C407(1.06)  LDD1540  [2]
 LDCM0303  AC41 HEK-293T C407(1.05)  LDD1542  [2]
 LDCM0304  AC42 HEK-293T C407(1.32)  LDD1543  [2]
 LDCM0305  AC43 HEK-293T C407(1.18)  LDD1544  [2]
 LDCM0306  AC44 HEK-293T C407(1.26)  LDD1545  [2]
 LDCM0308  AC46 HEK-293T C407(0.98)  LDD1547  [2]
 LDCM0309  AC47 HEK-293T C407(1.06)  LDD1548  [2]
 LDCM0311  AC49 HEK-293T C407(1.23)  LDD1550  [2]
 LDCM0313  AC50 HEK-293T C407(1.06)  LDD1552  [2]
 LDCM0314  AC51 HEK-293T C407(1.37)  LDD1553  [2]
 LDCM0315  AC52 HEK-293T C407(1.12)  LDD1554  [2]
 LDCM0317  AC54 HEK-293T C407(0.90)  LDD1556  [2]
 LDCM0318  AC55 HEK-293T C407(1.08)  LDD1557  [2]
 LDCM0320  AC57 HEK-293T C407(1.07)  LDD1559  [2]
 LDCM0321  AC58 HEK-293T C407(0.88)  LDD1560  [2]
 LDCM0322  AC59 HEK-293T C407(0.95)  LDD1561  [2]
 LDCM0323  AC6 HEK-293T C407(0.92)  LDD1562  [2]
 LDCM0324  AC60 HEK-293T C407(1.01)  LDD1563  [2]
 LDCM0326  AC62 HEK-293T C407(1.07)  LDD1565  [2]
 LDCM0327  AC63 HEK-293T C407(1.13)  LDD1566  [2]
 LDCM0334  AC7 HEK-293T C407(1.09)  LDD1568  [2]
 LDCM0356  AKOS034007680 HEK-293T C407(1.18)  LDD1570  [2]
 LDCM0368  CL10 HEK-293T C407(0.86)  LDD1572  [2]
 LDCM0369  CL100 HEK-293T C407(0.97)  LDD1573  [2]
 LDCM0371  CL102 HEK-293T C407(1.57)  LDD1575  [2]
 LDCM0372  CL103 HEK-293T C407(1.00)  LDD1576  [2]
 LDCM0373  CL104 HEK-293T C407(0.98)  LDD1577  [2]
 LDCM0375  CL106 HEK-293T C407(1.04)  LDD1579  [2]
 LDCM0376  CL107 HEK-293T C407(0.94)  LDD1580  [2]
 LDCM0377  CL108 HEK-293T C407(0.98)  LDD1581  [2]
 LDCM0379  CL11 HEK-293T C407(0.92)  LDD1583  [2]
 LDCM0380  CL110 HEK-293T C407(1.12)  LDD1584  [2]
 LDCM0381  CL111 HEK-293T C407(0.95)  LDD1585  [2]
 LDCM0382  CL112 HEK-293T C407(1.04)  LDD1586  [2]
 LDCM0384  CL114 HEK-293T C407(1.21)  LDD1588  [2]
 LDCM0385  CL115 HEK-293T C407(1.39)  LDD1589  [2]
 LDCM0386  CL116 HEK-293T C407(1.01)  LDD1590  [2]
 LDCM0388  CL118 HEK-293T C407(1.25)  LDD1592  [2]
 LDCM0389  CL119 HEK-293T C407(1.12)  LDD1593  [2]
 LDCM0391  CL120 HEK-293T C407(1.00)  LDD1595  [2]
 LDCM0393  CL122 HEK-293T C407(1.52)  LDD1597  [2]
 LDCM0394  CL123 HEK-293T C407(0.97)  LDD1598  [2]
 LDCM0395  CL124 HEK-293T C407(1.13)  LDD1599  [2]
 LDCM0397  CL126 HEK-293T C407(1.19)  LDD1601  [2]
 LDCM0398  CL127 HEK-293T C407(0.98)  LDD1602  [2]
 LDCM0399  CL128 HEK-293T C407(1.03)  LDD1603  [2]
 LDCM0401  CL14 HEK-293T C407(1.04)  LDD1605  [2]
 LDCM0402  CL15 HEK-293T C407(1.24)  LDD1606  [2]
 LDCM0403  CL16 HEK-293T C407(1.02)  LDD1607  [2]
 LDCM0404  CL17 HEK-293T C407(1.39)  LDD1608  [2]
 LDCM0405  CL18 HEK-293T C407(1.00)  LDD1609  [2]
 LDCM0406  CL19 HEK-293T C407(1.05)  LDD1610  [2]
 LDCM0407  CL2 HEK-293T C407(1.17)  LDD1611  [2]
 LDCM0408  CL20 HEK-293T C407(1.11)  LDD1612  [2]
 LDCM0410  CL22 HEK-293T C407(1.03)  LDD1614  [2]
 LDCM0411  CL23 HEK-293T C407(0.94)  LDD1615  [2]
 LDCM0414  CL26 HEK-293T C407(0.93)  LDD1618  [2]
 LDCM0415  CL27 HEK-293T C407(0.88)  LDD1619  [2]
 LDCM0416  CL28 HEK-293T C407(1.01)  LDD1620  [2]
 LDCM0417  CL29 HEK-293T C407(0.85)  LDD1621  [2]
 LDCM0418  CL3 HEK-293T C407(0.91)  LDD1622  [2]
 LDCM0419  CL30 HEK-293T C407(0.97)  LDD1623  [2]
 LDCM0420  CL31 HEK-293T C407(0.84)  LDD1624  [2]
 LDCM0421  CL32 HEK-293T C407(1.00)  LDD1625  [2]
 LDCM0423  CL34 HEK-293T C407(0.91)  LDD1627  [2]
 LDCM0424  CL35 HEK-293T C407(0.85)  LDD1628  [2]
 LDCM0428  CL39 HEK-293T C407(1.01)  LDD1632  [2]
 LDCM0429  CL4 HEK-293T C407(1.03)  LDD1633  [2]
 LDCM0430  CL40 HEK-293T C407(0.95)  LDD1634  [2]
 LDCM0431  CL41 HEK-293T C407(1.28)  LDD1635  [2]
 LDCM0432  CL42 HEK-293T C407(0.99)  LDD1636  [2]
 LDCM0433  CL43 HEK-293T C407(0.59)  LDD1637  [2]
 LDCM0434  CL44 HEK-293T C407(0.95)  LDD1638  [2]
 LDCM0436  CL46 HEK-293T C407(0.85)  LDD1640  [2]
 LDCM0437  CL47 HEK-293T C407(0.80)  LDD1641  [2]
 LDCM0440  CL5 HEK-293T C407(1.06)  LDD1644  [2]
 LDCM0441  CL50 HEK-293T C407(1.21)  LDD1645  [2]
 LDCM0443  CL52 HEK-293T C407(0.94)  LDD1646  [2]
 LDCM0444  CL53 HEK-293T C407(1.21)  LDD1647  [2]
 LDCM0445  CL54 HEK-293T C407(1.21)  LDD1648  [2]
 LDCM0446  CL55 HEK-293T C407(0.87)  LDD1649  [2]
 LDCM0447  CL56 HEK-293T C407(0.92)  LDD1650  [2]
 LDCM0449  CL58 HEK-293T C407(0.94)  LDD1652  [2]
 LDCM0450  CL59 HEK-293T C407(0.93)  LDD1653  [2]
 LDCM0451  CL6 HEK-293T C407(1.08)  LDD1654  [2]
 LDCM0454  CL62 HEK-293T C407(0.98)  LDD1657  [2]
 LDCM0455  CL63 HEK-293T C407(1.11)  LDD1658  [2]
 LDCM0456  CL64 HEK-293T C407(1.04)  LDD1659  [2]
 LDCM0457  CL65 HEK-293T C407(1.17)  LDD1660  [2]
 LDCM0458  CL66 HEK-293T C407(1.02)  LDD1661  [2]
 LDCM0459  CL67 HEK-293T C407(0.76)  LDD1662  [2]
 LDCM0460  CL68 HEK-293T C407(1.07)  LDD1663  [2]
 LDCM0462  CL7 HEK-293T C407(0.79)  LDD1665  [2]
 LDCM0463  CL70 HEK-293T C407(0.91)  LDD1666  [2]
 LDCM0464  CL71 HEK-293T C407(1.06)  LDD1667  [2]
 LDCM0467  CL74 HEK-293T C407(1.14)  LDD1670  [2]
 LDCM0469  CL76 HEK-293T C407(1.07)  LDD1672  [2]
 LDCM0470  CL77 HEK-293T C407(1.26)  LDD1673  [2]
 LDCM0471  CL78 HEK-293T C407(1.05)  LDD1674  [2]
 LDCM0472  CL79 HEK-293T C407(0.80)  LDD1675  [2]
 LDCM0473  CL8 HEK-293T C407(2.02)  LDD1676  [2]
 LDCM0474  CL80 HEK-293T C407(1.08)  LDD1677  [2]
 LDCM0476  CL82 HEK-293T C407(1.02)  LDD1679  [2]
 LDCM0477  CL83 HEK-293T C407(1.02)  LDD1680  [2]
 LDCM0480  CL86 HEK-293T C407(1.06)  LDD1683  [2]
 LDCM0481  CL87 HEK-293T C407(0.97)  LDD1684  [2]
 LDCM0482  CL88 HEK-293T C407(1.04)  LDD1685  [2]
 LDCM0483  CL89 HEK-293T C407(0.90)  LDD1686  [2]
 LDCM0485  CL90 HEK-293T C407(1.95)  LDD1688  [2]
 LDCM0486  CL91 HEK-293T C407(0.77)  LDD1689  [2]
 LDCM0487  CL92 HEK-293T C407(0.95)  LDD1690  [2]
 LDCM0489  CL94 HEK-293T C407(1.27)  LDD1692  [2]
 LDCM0490  CL95 HEK-293T C407(1.33)  LDD1693  [2]
 LDCM0493  CL98 HEK-293T C407(0.97)  LDD1696  [2]
 LDCM0494  CL99 HEK-293T C407(1.15)  LDD1697  [2]
 LDCM0495  E2913 HEK-293T C407(1.00)  LDD1698  [2]
 LDCM0468  Fragment33 HEK-293T C407(1.16)  LDD1671  [2]
 LDCM0427  Fragment51 HEK-293T C407(0.97)  LDD1631  [2]

References

1 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060