Details of the Target

General Information of Target

Target ID LDTP08263
Target Name Fermitin family homolog 3 (FERMT3)
Gene Name FERMT3
Gene ID 83706
Synonyms
KIND3; MIG2B; URP2; Fermitin family homolog 3; Kindlin-3; MIG2-like protein; Unc-112-related protein 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAGMKTASGDYIDSSWELRVFVGEEDPEAESVTLRVTGESHIGGVLLKIVEQINRKQDWS
DHAIWWEQKRQWLLQTHWTLDKYGILADARLFFGPQHRPVILRLPNRRALRLRASFSQPL
FQAVAAICRLLSIRHPEELSLLRAPEKKEKKKKEKEPEEELYDLSKVVLAGGVAPALFRG
MPAHFSDSAQTEACYHMLSRPQPPPDPLLLQRLPRPSSLSDKTQLHSRWLDSSRCLMQQG
IKAGDALWLRFKYYSFFDLDPKTDPVRLTQLYEQARWDLLLEEIDCTEEEMMVFAALQYH
INKLSQSGEVGEPAGTDPGLDDLDVALSNLEVKLEGSAPTDVLDSLTTIPELKDHLRIFR
IPRRPRKLTLKGYRQHWVVFKETTLSYYKSQDEAPGDPIQQLNLKGCEVVPDVNVSGQKF
CIKLLVPSPEGMSEIYLRCQDEQQYARWMAGCRLASKGRTMADSSYTSEVQAILAFLSLQ
RTGSGGPGNHPHGPDASAEGLNPYGLVAPRFQRKFKAKQLTPRILEAHQNVAQLSLAEAQ
LRFIQAWQSLPDFGISYVMVRFKGSRKDEILGIANNRLIRIDLAVGDVVKTWRFSNMRQW
NVNWDIRQVAIEFDEHINVAFSCVSASCRIVHEYIGGYIFLSTRERARGEELDEDLFLQL
TGGHEAF
Target Bioclass
Other
Family
Kindlin family
Subcellular location
Cell projection, podosome
Function
Plays a central role in cell adhesion in hematopoietic cells. Acts by activating the integrin beta-1-3 (ITGB1, ITGB2 and ITGB3). Required for integrin-mediated platelet adhesion and leukocyte adhesion to endothelial cells. Required for activation of integrin beta-2 (ITGB2) in polymorphonuclear granulocytes (PMNs).; Isoform 2 may act as a repressor of NF-kappa-B and apoptosis.
Uniprot ID
Q86UX7
Ensemble ID
ENST00000279227.10
HGNC ID
HGNC:23151

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
A-EBA
 Probe Info  		3.07  LDD0215  [1]
TH211
 Probe Info  		Y436(20.00); Y162(20.00); Y11(18.94)  LDD0260  [2]
STPyne
 Probe Info  		K371(5.43)  LDD2218  [3]
DBIA
 Probe Info  		C439(2.26); C407(4.06)  LDD3333  [4]
IPM
 Probe Info  		C235(2.13)  LDD1702  [5]
4-Iodoacetamidophenylacetylene
 Probe Info  		C128(0.00); C235(0.00); C439(0.00); C407(0.00)  LDD0038  [6]
IA-alkyne
 Probe Info  		C128(0.00); C235(0.00); C439(0.00); C407(0.00)  LDD0036  [6]
IPIAA_H
 Probe Info  		C439(0.00); C128(0.00)  LDD0030  [7]
IPIAA_L
 Probe Info  		C128(0.00); C439(0.00); C407(0.00); C194(0.00)  LDD0031  [7]
Lodoacetamide azide
 Probe Info  		C128(0.00); C235(0.00); C407(0.00); C194(0.00)  LDD0037  [6]
Compound 10
 Probe Info  		N.A.  LDD2216  [8]
PAL-AfBPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe3
 Probe Info  		5.17  LDD0465  [9]
VE-P
 Probe Info  		N.A.  LDD0396  [10]
DA-2
 Probe Info  		N.A.  LDD0070  [11]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C235(1.14)  LDD1508  [12]
 LDCM0277  AC18 HEK-293T C235(1.08)  LDD1516  [12]
 LDCM0279  AC2 HEK-293T C235(1.10)  LDD1518  [12]
 LDCM0286  AC26 HEK-293T C235(1.04)  LDD1525  [12]
 LDCM0295  AC34 HEK-293T C235(1.23)  LDD1534  [12]
 LDCM0304  AC42 HEK-293T C235(1.10)  LDD1543  [12]
 LDCM0313  AC50 HEK-293T C235(1.11)  LDD1552  [12]
 LDCM0321  AC58 HEK-293T C235(1.10)  LDD1560  [12]
 LDCM0372  CL103 HEK-293T C235(0.95)  LDD1576  [12]
 LDCM0376  CL107 HEK-293T C235(1.03)  LDD1580  [12]
 LDCM0381  CL111 HEK-293T C235(0.99)  LDD1585  [12]
 LDCM0385  CL115 HEK-293T C235(0.91)  LDD1589  [12]
 LDCM0389  CL119 HEK-293T C235(0.93)  LDD1593  [12]
 LDCM0394  CL123 HEK-293T C235(0.89)  LDD1598  [12]
 LDCM0398  CL127 HEK-293T C235(0.90)  LDD1602  [12]
 LDCM0402  CL15 HEK-293T C235(1.07)  LDD1606  [12]
 LDCM0405  CL18 HEK-293T C235(1.45)  LDD1609  [12]
 LDCM0415  CL27 HEK-293T C235(0.97)  LDD1619  [12]
 LDCM0418  CL3 HEK-293T C235(1.20)  LDD1622  [12]
 LDCM0419  CL30 HEK-293T C235(1.20)  LDD1623  [12]
 LDCM0428  CL39 HEK-293T C235(0.90)  LDD1632  [12]
 LDCM0432  CL42 HEK-293T C235(1.14)  LDD1636  [12]
 LDCM0445  CL54 HEK-293T C235(1.07)  LDD1648  [12]
 LDCM0451  CL6 HEK-293T C235(1.42)  LDD1654  [12]
 LDCM0455  CL63 HEK-293T C235(0.94)  LDD1658  [12]
 LDCM0458  CL66 HEK-293T C235(1.17)  LDD1661  [12]
 LDCM0471  CL78 HEK-293T C235(1.22)  LDD1674  [12]
 LDCM0481  CL87 HEK-293T C235(0.94)  LDD1684  [12]
 LDCM0485  CL90 HEK-293T C235(0.93)  LDD1688  [12]
 LDCM0494  CL99 HEK-293T C235(0.85)  LDD1697  [12]
 LDCM0495  E2913 HEK-293T C235(0.87)  LDD1698  [12]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C235(2.13)  LDD1702  [5]
 LDCM0625  F8 Ramos C439(1.24); C407(0.94); C128(0.55); C235(3.38)  LDD2187  [13]
 LDCM0572  Fragment10 Ramos C439(0.51); C407(0.48); C128(0.52); C235(0.22)  LDD2189  [13]
 LDCM0573  Fragment11 Ramos C439(1.44); C407(1.51); C128(0.15); C235(1.17)  LDD2190  [13]
 LDCM0574  Fragment12 Ramos C439(0.53); C407(0.38); C128(0.64); C235(0.25)  LDD2191  [13]
 LDCM0575  Fragment13 Ramos C439(0.99); C407(1.09); C128(0.69); C235(1.27)  LDD2192  [13]
 LDCM0576  Fragment14 Ramos C439(0.36); C407(0.72); C128(0.70); C235(0.70)  LDD2193  [13]
 LDCM0579  Fragment20 Ramos C439(0.91); C407(0.45); C128(0.46); C235(0.29)  LDD2194  [13]
 LDCM0580  Fragment21 Ramos C439(0.89); C407(1.00); C128(0.75); C235(1.19)  LDD2195  [13]
 LDCM0582  Fragment23 Ramos C439(0.18); C407(0.16); C128(0.77); C235(0.23)  LDD2196  [13]
 LDCM0578  Fragment27 Ramos C439(1.04); C407(1.15); C128(0.88); C235(0.61)  LDD2197  [13]
 LDCM0586  Fragment28 Ramos C439(0.43); C407(0.77); C128(1.05); C235(0.34)  LDD2198  [13]
 LDCM0588  Fragment30 Ramos C439(0.33); C407(0.34); C128(0.55); C235(1.75)  LDD2199  [13]
 LDCM0589  Fragment31 Ramos C439(1.18); C407(1.03); C128(1.35); C235(2.44)  LDD2200  [13]
 LDCM0590  Fragment32 Ramos C439(0.39); C407(0.29); C128(0.41); C235(0.21)  LDD2201  [13]
 LDCM0468  Fragment33 HEK-293T C235(0.94)  LDD1671  [12]
 LDCM0596  Fragment38 Ramos C439(0.94); C407(0.80); C128(0.76); C235(0.93)  LDD2203  [13]
 LDCM0566  Fragment4 Ramos C439(0.59); C407(0.55); C128(0.65); C235(0.93)  LDD2184  [13]
 LDCM0610  Fragment52 Ramos C439(1.16); C407(1.32); C128(0.77); C235(0.78)  LDD2204  [13]
 LDCM0614  Fragment56 Ramos C439(2.89); C407(1.31); C128(1.09); C235(1.84)  LDD2205  [13]
 LDCM0569  Fragment7 Ramos C439(0.21); C407(0.26); C128(0.44); C235(0.54)  LDD2186  [13]
 LDCM0571  Fragment9 Ramos C439(0.32); C407(0.39); C128(0.53); C235(0.29)  LDD2188  [13]
 LDCM0022  KB02 Ramos C439(0.21); C407(0.27); C128(0.36); C235(0.59)  LDD2182  [13]
 LDCM0023  KB03 Ramos C439(0.28); C407(0.32); C128(0.46); C235(0.71)  LDD2183  [13]
 LDCM0024  KB05 MOLM-13 C439(2.26); C407(4.06)  LDD3333  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Pre-mRNA-splicing factor 18 (PRPF18) PRP18 family Q99633
Ankyrin repeat domain-containing protein 29 (ANKRD29) . Q8N6D5

References

1 2-Ethynylbenzaldehyde-Based, Lysine-Targeting Irreversible Covalent Inhibitors for Protein Kinases and Nonkinases. J Am Chem Soc. 2023 Feb 12. doi: 10.1021/jacs.2c11595. Online ahead of print.
Mass spectrometry data entry: PXD037665
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 Global profiling of lysine reactivity and ligandability in the human proteome. Nat Chem. 2017 Dec;9(12):1181-1190. doi: 10.1038/nchem.2826. Epub 2017 Jul 31.
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
6 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
7 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
8 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
9 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
10 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
11 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.
12 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
13 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578