Details of the Target

General Information of Target

Target ID LDTP07988
Target Name Carbohydrate sulfotransferase 15 (CHST15)
Gene Name CHST15
Gene ID 51363
Synonyms
BRAG; GALNAC4S6ST; KIAA0598; Carbohydrate sulfotransferase 15; EC 2.8.2.33; B-cell RAG-associated gene protein; hBRAG; N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase; GalNAc4S-6ST
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MRHCINCCIQLLPDGAHKQQVNCQGGPHHGHQACPTCKGENKILFRVDSKQMNLLAVLEV
RTEGNENWGGFLRFKKGKRCSLVFGLIIMTLVMASYILSGAHQELLISSPFHYGGFPSNP
SLMDSENPSDTKEHHHQSSVNNISYMKDYPSIKLIINSITTRIEFTTRQLPDLEDLKKQE
LHMFSVIPNKFLPNSKSPCWYEEFSGQNTTDPYLTNSYVLYSKRFRSTFDALRKAFWGHL
AHAHGKHFRLRCLPHFYIIGQPKCGTTDLYDRLRLHPEVKFSAIKEPHWWTRKRFGIVRL
RDGLRDRYPVEDYLDLFDLAAHQIHQGLQASSAKEQSKMNTIIIGEASASTMWDNNAWTF
FYDNSTDGEPPFLTQDFIHAFQPNARLIVMLRDPVERLYSDYLYFASSNKSADDFHEKVT
EALQLFENCMLDYSLRACVYNNTLNNAMPVRLQVGLYAVYLLDWLSVFDKQQFLILRLED
HASNVKYTMHKVFQFLNLGPLSEKQEALMTKSPASNARRPEDRNLGPMWPITQKILRDFY
RPFNARLAQVLADEAFAWKTT
Target Type
Literature-reported
Target Bioclass
Enzyme
Family
Sulfotransferase 1 family
Subcellular location
Golgi apparatus membrane
Function
Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo.
TTD ID
T07944
Uniprot ID
Q7LFX5
DrugMap ID
TTFK7E1
Ensemble ID
ENST00000346248.7
HGNC ID
HGNC:18137

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
EFO27 Deletion: p.W68GfsTer13 .
HCT116 SNV: p.A231T .
HCT15 SNV: p.R249H .
JURKAT SNV: p.S227F .
LNCaP clone FGC SNV: p.E503A .
NCIH1155 SNV: p.R518Q .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C264(1.27)  LDD0547  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0230  AC113 HCT 116 C264(1.27)  LDD0547  [1]
 LDCM0231  AC114 HCT 116 C264(0.99)  LDD0548  [1]
 LDCM0232  AC115 HCT 116 C264(0.81)  LDD0549  [1]
 LDCM0233  AC116 HCT 116 C264(1.03)  LDD0550  [1]
 LDCM0234  AC117 HCT 116 C264(0.81)  LDD0551  [1]
 LDCM0235  AC118 HCT 116 C264(0.96)  LDD0552  [1]
 LDCM0236  AC119 HCT 116 C264(1.04)  LDD0553  [1]
 LDCM0238  AC120 HCT 116 C264(0.62)  LDD0555  [1]
 LDCM0239  AC121 HCT 116 C264(0.91)  LDD0556  [1]
 LDCM0240  AC122 HCT 116 C264(0.71)  LDD0557  [1]
 LDCM0241  AC123 HCT 116 C264(0.70)  LDD0558  [1]
 LDCM0242  AC124 HCT 116 C264(1.15)  LDD0559  [1]
 LDCM0243  AC125 HCT 116 C264(0.86)  LDD0560  [1]
 LDCM0244  AC126 HCT 116 C264(0.81)  LDD0561  [1]
 LDCM0245  AC127 HCT 116 C264(0.99)  LDD0562  [1]
 LDCM0285  AC25 HCT 116 C264(1.30)  LDD0602  [1]
 LDCM0286  AC26 HCT 116 C264(1.08)  LDD0603  [1]
 LDCM0287  AC27 HCT 116 C264(1.14)  LDD0604  [1]
 LDCM0288  AC28 HCT 116 C264(1.01)  LDD0605  [1]
 LDCM0289  AC29 HCT 116 C264(1.24)  LDD0606  [1]
 LDCM0291  AC30 HCT 116 C264(1.13)  LDD0608  [1]
 LDCM0292  AC31 HCT 116 C264(1.07)  LDD0609  [1]
 LDCM0293  AC32 HCT 116 C264(1.27)  LDD0610  [1]
 LDCM0294  AC33 HCT 116 C264(1.09)  LDD0611  [1]
 LDCM0295  AC34 HCT 116 C264(1.21)  LDD0612  [1]
 LDCM0296  AC35 HCT 116 C264(0.73)  LDD0613  [1]
 LDCM0297  AC36 HCT 116 C264(0.83)  LDD0614  [1]
 LDCM0298  AC37 HCT 116 C264(1.19)  LDD0615  [1]
 LDCM0299  AC38 HCT 116 C264(0.90)  LDD0616  [1]
 LDCM0300  AC39 HCT 116 C264(0.80)  LDD0617  [1]
 LDCM0302  AC40 HCT 116 C264(0.92)  LDD0619  [1]
 LDCM0303  AC41 HCT 116 C264(1.27)  LDD0620  [1]
 LDCM0304  AC42 HCT 116 C264(0.98)  LDD0621  [1]
 LDCM0305  AC43 HCT 116 C264(1.03)  LDD0622  [1]
 LDCM0306  AC44 HCT 116 C264(1.21)  LDD0623  [1]
 LDCM0307  AC45 HCT 116 C264(1.29)  LDD0624  [1]
 LDCM0308  AC46 HCT 116 C264(0.69)  LDD0625  [1]
 LDCM0309  AC47 HCT 116 C264(0.86)  LDD0626  [1]
 LDCM0310  AC48 HCT 116 C264(0.99)  LDD0627  [1]
 LDCM0311  AC49 HCT 116 C264(1.03)  LDD0628  [1]
 LDCM0313  AC50 HCT 116 C264(1.03)  LDD0630  [1]
 LDCM0314  AC51 HCT 116 C264(1.02)  LDD0631  [1]
 LDCM0315  AC52 HCT 116 C264(0.96)  LDD0632  [1]
 LDCM0316  AC53 HCT 116 C264(0.97)  LDD0633  [1]
 LDCM0317  AC54 HCT 116 C264(0.97)  LDD0634  [1]
 LDCM0318  AC55 HCT 116 C264(1.03)  LDD0635  [1]
 LDCM0319  AC56 HCT 116 C264(1.04)  LDD0636  [1]
 LDCM0382  CL112 HCT 116 C264(1.02)  LDD0699  [1]
 LDCM0383  CL113 HCT 116 C264(1.18)  LDD0700  [1]
 LDCM0384  CL114 HCT 116 C264(1.60)  LDD0701  [1]
 LDCM0385  CL115 HCT 116 C264(1.06)  LDD0702  [1]
 LDCM0386  CL116 HCT 116 C264(1.10)  LDD0703  [1]
 LDCM0387  CL117 HCT 116 C264(0.82)  LDD0704  [1]
 LDCM0388  CL118 HCT 116 C264(0.83)  LDD0705  [1]
 LDCM0389  CL119 HCT 116 C264(1.09)  LDD0706  [1]
 LDCM0391  CL120 HCT 116 C264(1.20)  LDD0708  [1]
 LDCM0392  CL121 HCT 116 C264(0.89)  LDD0709  [1]
 LDCM0393  CL122 HCT 116 C264(0.94)  LDD0710  [1]
 LDCM0394  CL123 HCT 116 C264(0.70)  LDD0711  [1]
 LDCM0395  CL124 HCT 116 C264(1.12)  LDD0712  [1]
 LDCM0453  CL61 HCT 116 C264(1.61)  LDD0770  [1]
 LDCM0454  CL62 HCT 116 C264(1.13)  LDD0771  [1]
 LDCM0455  CL63 HCT 116 C264(1.86)  LDD0772  [1]
 LDCM0456  CL64 HCT 116 C264(1.56)  LDD0773  [1]
 LDCM0457  CL65 HCT 116 C264(1.10)  LDD0774  [1]
 LDCM0458  CL66 HCT 116 C264(1.34)  LDD0775  [1]
 LDCM0459  CL67 HCT 116 C264(1.37)  LDD0776  [1]
 LDCM0460  CL68 HCT 116 C264(1.06)  LDD0777  [1]
 LDCM0461  CL69 HCT 116 C264(1.14)  LDD0778  [1]
 LDCM0463  CL70 HCT 116 C264(1.45)  LDD0780  [1]
 LDCM0464  CL71 HCT 116 C264(1.47)  LDD0781  [1]
 LDCM0465  CL72 HCT 116 C264(1.24)  LDD0782  [1]
 LDCM0466  CL73 HCT 116 C264(1.34)  LDD0783  [1]
 LDCM0467  CL74 HCT 116 C264(2.15)  LDD0784  [1]
 LDCM0469  CL76 HCT 116 C264(1.12)  LDD0786  [1]
 LDCM0470  CL77 HCT 116 C264(1.52)  LDD0787  [1]
 LDCM0471  CL78 HCT 116 C264(1.03)  LDD0788  [1]
 LDCM0472  CL79 HCT 116 C264(1.08)  LDD0789  [1]
 LDCM0474  CL80 HCT 116 C264(1.30)  LDD0791  [1]
 LDCM0475  CL81 HCT 116 C264(1.34)  LDD0792  [1]
 LDCM0476  CL82 HCT 116 C264(1.16)  LDD0793  [1]
 LDCM0477  CL83 HCT 116 C264(1.30)  LDD0794  [1]
 LDCM0478  CL84 HCT 116 C264(2.06)  LDD0795  [1]
 LDCM0479  CL85 HCT 116 C264(1.12)  LDD0796  [1]
 LDCM0480  CL86 HCT 116 C264(0.85)  LDD0797  [1]
 LDCM0481  CL87 HCT 116 C264(1.01)  LDD0798  [1]
 LDCM0482  CL88 HCT 116 C264(1.11)  LDD0799  [1]
 LDCM0483  CL89 HCT 116 C264(1.09)  LDD0800  [1]
 LDCM0485  CL90 HCT 116 C264(1.23)  LDD0802  [1]
 LDCM0468  Fragment33 HCT 116 C264(1.30)  LDD0785  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Calnexin (CANX) Calreticulin family P27824

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.