Details of the Target

General Information of Target

Target ID LDTP07781
Target Name Nesprin-3 (SYNE3)
Gene Name SYNE3
Gene ID 161176
Synonyms
C14orf139; C14orf49; LINC00341; Nesprin-3; KASH domain-containing protein 3; KASH3; Nuclear envelope spectrin repeat protein 3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTQQPQDDFDRSVEDAQAWMKAVQDQLQVNDNTQGPRAALEARLWETEKICQLEPEGRVR
VDLVLRMAEALLACCPGDQKPGILARLKDIKAQWEETVTYMTHCHSRIEWVWLHWSEYLL
ARDEFYRWFQKMMVTLEPHIELQLGLKEKQWQLSHAQVLLHNVDNQAVLLDRLLEEAASL
FNRIGDPSVDEDAQKRMKAEYDAVKAKAQKRVDLLEQVAREHEEYQAGVDEFQLWLKAVV
EKVNGCLGRNCKLPITQRLSTLQDIAKDFPRGEESLETLEEQSAGVIRNTSPLGAEKITG
ELEEMRKVLEKLRALWEEEEERLRGLLRSRGAWEQQIKQLEAELSEFRMVLQRLAQEGLQ
PAAKAGTEDELVAHWRRYSATRAALASEEPRVDRLQAQLKELIVFPHNLKPLSDSVIATI
QEYQSLKVKSARLRNAAAVELWQHFQRPLQDLQLWKALAQRLLEVTASLPDLPSLHTFLP
QIEAALMESSRLKELLTMLQLKKDLLIGIFGQERATALLEQVAGSMRDRDLLHNSLLQRK
SKLQSLLAQHKDFGAAFEPLQRKLLDLQVRVQAEKGLQRDLPGKQAQLSRLQGLQEEGLD
LGAQMEAARPLVQENPNHQHKMDQLSSDFQALQRSLEDLVDRCRQSVQEHCTFSHQLLEL
RQWIVVTTQKLEAHRGEAGPGDAESQEAEFERLVAEFPEKEAQLSLVEAQGWLVMEKSSP
EGAAVVQEELRELAESWRALRLLEESLLSLIRNWHLQRMEVDSGKKMVFTNNIPKSGFLI
NPMDPIPRHRRRANLLQEEEGSHEDFSQLLRNFGQWLQVENSKLVRIIAMRTSTAEDLRT
RKSKLQELEARVPEGQHLFENLLRLGPARGTSDELEDLRYQWMLYKSKLKDSGHLLTQSS
PGEPTGFQKTRRWRGLGSLFRRACCVALPLQLLLLLFLLLLFLLPIREEDRSCTLANNFA
RSFTLMLRYNGPPPT
Target Bioclass
Other
Family
Nesprin family
Subcellular location
Nucleus outer membrane
Function
As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells.
Uniprot ID
Q6ZMZ3
Ensemble ID
ENST00000334258.9
HGNC ID
HGNC:19861

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
DU145 SNV: p.K267N .
HCT116 SNV: p.R529I .
HCT15 SNV: p.Q4H .
HT115 SNV: p.E301Ter .
IGR37 SNV: p.A436V DBIA    Probe Info 
IGR39 SNV: p.A436V DBIA    Probe Info 
LNCaP clone FGC SNV: p.R947G .
MEWO SNV: p.Q613Ter DBIA    Probe Info 
MFE319 SNV: p.G366E .
MOLM13 SNV: p.A332T DBIA    Probe Info 
NCIH1793 SNV: p.D580N .
NCIH196 SNV: p.V13L .
NCIH2291 SNV: p.A603E .
NCIH358 SNV: p.E341D .
REH SNV: p.H803R DBIA    Probe Info 
RKO SNV: p.E696G .
TE1 SNV: p.G294D .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K242(8.33)  LDD0277  [1]
DBIA
 Probe Info  		C51(3.42); C246(1.95)  LDD3332  [2]
BTD
 Probe Info  		C246(1.14)  LDD2117  [3]
Alkyne-RA190
 Probe Info  		2.61  LDD0300  [4]
IA-alkyne
 Probe Info  		C651(6.10)  LDD1703  [5]
IPM
 Probe Info  		C953(3.88)  LDD1701  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C246(1.14)  LDD2117  [3]
 LDCM0215  AC10 HEK-293T C246(1.08)  LDD1508  [6]
 LDCM0226  AC11 HEK-293T C246(1.10); C51(1.04)  LDD1509  [6]
 LDCM0237  AC12 HEK-293T C246(0.98)  LDD1510  [6]
 LDCM0259  AC14 HEK-293T C51(0.87)  LDD1512  [6]
 LDCM0277  AC18 HEK-293T C246(1.03)  LDD1516  [6]
 LDCM0278  AC19 HEK-293T C246(1.20); C51(1.27)  LDD1517  [6]
 LDCM0279  AC2 HEK-293T C246(1.38)  LDD1518  [6]
 LDCM0280  AC20 HEK-293T C246(0.82)  LDD1519  [6]
 LDCM0281  AC21 HEK-293T C51(1.14)  LDD1520  [6]
 LDCM0282  AC22 HEK-293T C51(0.90)  LDD1521  [6]
 LDCM0284  AC24 HEK-293T C51(0.88)  LDD1523  [6]
 LDCM0286  AC26 HEK-293T C246(0.95)  LDD1525  [6]
 LDCM0287  AC27 HEK-293T C246(1.03); C51(1.05)  LDD1526  [6]
 LDCM0288  AC28 HEK-293T C246(0.80)  LDD1527  [6]
 LDCM0289  AC29 HEK-293T C51(1.11)  LDD1528  [6]
 LDCM0290  AC3 HEK-293T C246(1.06); C51(0.98)  LDD1529  [6]
 LDCM0291  AC30 HEK-293T C51(0.98)  LDD1530  [6]
 LDCM0293  AC32 HEK-293T C51(0.80)  LDD1532  [6]
 LDCM0295  AC34 HEK-293T C246(0.97)  LDD1534  [6]
 LDCM0296  AC35 HEK-293T C246(0.99); C51(0.98)  LDD1535  [6]
 LDCM0297  AC36 HEK-293T C246(0.98)  LDD1536  [6]
 LDCM0298  AC37 HEK-293T C51(1.10)  LDD1537  [6]
 LDCM0299  AC38 HEK-293T C51(0.97)  LDD1538  [6]
 LDCM0301  AC4 HEK-293T C246(0.90)  LDD1540  [6]
 LDCM0302  AC40 HEK-293T C51(0.89)  LDD1541  [6]
 LDCM0304  AC42 HEK-293T C246(1.16)  LDD1543  [6]
 LDCM0305  AC43 HEK-293T C246(1.07); C51(0.99)  LDD1544  [6]
 LDCM0306  AC44 HEK-293T C246(0.84)  LDD1545  [6]
 LDCM0307  AC45 HEK-293T C51(0.95)  LDD1546  [6]
 LDCM0308  AC46 HEK-293T C51(0.95)  LDD1547  [6]
 LDCM0310  AC48 HEK-293T C51(0.94)  LDD1549  [6]
 LDCM0312  AC5 HEK-293T C51(1.15)  LDD1551  [6]
 LDCM0313  AC50 HEK-293T C246(1.03)  LDD1552  [6]
 LDCM0314  AC51 HEK-293T C246(0.97); C51(0.90)  LDD1553  [6]
 LDCM0315  AC52 HEK-293T C246(0.87)  LDD1554  [6]
 LDCM0316  AC53 HEK-293T C51(0.94)  LDD1555  [6]
 LDCM0317  AC54 HEK-293T C51(0.96)  LDD1556  [6]
 LDCM0319  AC56 HEK-293T C51(1.00)  LDD1558  [6]
 LDCM0321  AC58 HEK-293T C246(1.10)  LDD1560  [6]
 LDCM0322  AC59 HEK-293T C246(1.11); C51(1.04)  LDD1561  [6]
 LDCM0323  AC6 HEK-293T C51(0.83)  LDD1562  [6]
 LDCM0324  AC60 HEK-293T C246(0.97)  LDD1563  [6]
 LDCM0325  AC61 HEK-293T C51(1.02)  LDD1564  [6]
 LDCM0326  AC62 HEK-293T C51(0.83)  LDD1565  [6]
 LDCM0328  AC64 HEK-293T C51(0.90)  LDD1567  [6]
 LDCM0345  AC8 HEK-293T C51(1.02)  LDD1569  [6]
 LDCM0248  AKOS034007472 HEK-293T C51(1.14)  LDD1511  [6]
 LDCM0275  AKOS034007705 HEK-293T C51(0.95)  LDD1514  [6]
 LDCM0368  CL10 HEK-293T C51(1.54)  LDD1572  [6]
 LDCM0372  CL103 HEK-293T C246(0.97)  LDD1576  [6]
 LDCM0376  CL107 HEK-293T C246(0.99)  LDD1580  [6]
 LDCM0381  CL111 HEK-293T C246(1.23)  LDD1585  [6]
 LDCM0385  CL115 HEK-293T C246(1.29)  LDD1589  [6]
 LDCM0389  CL119 HEK-293T C246(1.01)  LDD1593  [6]
 LDCM0390  CL12 HEK-293T C51(0.97)  LDD1594  [6]
 LDCM0394  CL123 HEK-293T C246(1.02)  LDD1598  [6]
 LDCM0398  CL127 HEK-293T C246(1.03)  LDD1602  [6]
 LDCM0402  CL15 HEK-293T C246(1.64)  LDD1606  [6]
 LDCM0405  CL18 HEK-293T C246(1.10)  LDD1609  [6]
 LDCM0406  CL19 HEK-293T C246(1.03); C51(1.13)  LDD1610  [6]
 LDCM0408  CL20 HEK-293T C246(0.77)  LDD1612  [6]
 LDCM0409  CL21 HEK-293T C51(1.18)  LDD1613  [6]
 LDCM0410  CL22 HEK-293T C51(1.15)  LDD1614  [6]
 LDCM0412  CL24 HEK-293T C51(1.04)  LDD1616  [6]
 LDCM0415  CL27 HEK-293T C246(1.08)  LDD1619  [6]
 LDCM0418  CL3 HEK-293T C246(1.20)  LDD1622  [6]
 LDCM0419  CL30 HEK-293T C246(1.15)  LDD1623  [6]
 LDCM0420  CL31 HEK-293T C246(1.21); C51(1.13)  LDD1624  [6]
 LDCM0421  CL32 HEK-293T C246(0.71)  LDD1625  [6]
 LDCM0422  CL33 HEK-293T C51(1.10)  LDD1626  [6]
 LDCM0423  CL34 HEK-293T C51(1.20)  LDD1627  [6]
 LDCM0425  CL36 HEK-293T C51(1.04)  LDD1629  [6]
 LDCM0428  CL39 HEK-293T C246(1.00)  LDD1632  [6]
 LDCM0432  CL42 HEK-293T C246(1.10)  LDD1636  [6]
 LDCM0433  CL43 HEK-293T C246(0.98); C51(1.21)  LDD1637  [6]
 LDCM0434  CL44 HEK-293T C246(0.85)  LDD1638  [6]
 LDCM0435  CL45 HEK-293T C51(1.25)  LDD1639  [6]
 LDCM0436  CL46 HEK-293T C51(0.93)  LDD1640  [6]
 LDCM0438  CL48 HEK-293T C51(1.15)  LDD1642  [6]
 LDCM0445  CL54 HEK-293T C246(0.87)  LDD1648  [6]
 LDCM0446  CL55 HEK-293T C246(0.92); C51(1.07)  LDD1649  [6]
 LDCM0447  CL56 HEK-293T C246(0.84)  LDD1650  [6]
 LDCM0448  CL57 HEK-293T C51(1.12)  LDD1651  [6]
 LDCM0449  CL58 HEK-293T C51(0.97)  LDD1652  [6]
 LDCM0451  CL6 HEK-293T C246(1.13)  LDD1654  [6]
 LDCM0452  CL60 HEK-293T C51(0.86)  LDD1655  [6]
 LDCM0455  CL63 HEK-293T C246(1.15)  LDD1658  [6]
 LDCM0458  CL66 HEK-293T C246(0.89)  LDD1661  [6]
 LDCM0459  CL67 HEK-293T C246(1.21); C51(1.17)  LDD1662  [6]
 LDCM0460  CL68 HEK-293T C246(0.86)  LDD1663  [6]
 LDCM0461  CL69 HEK-293T C51(1.32)  LDD1664  [6]
 LDCM0462  CL7 HEK-293T C246(1.06); C51(1.01)  LDD1665  [6]
 LDCM0463  CL70 HEK-293T C51(1.00)  LDD1666  [6]
 LDCM0465  CL72 HEK-293T C51(0.89)  LDD1668  [6]
 LDCM0471  CL78 HEK-293T C246(1.08)  LDD1674  [6]
 LDCM0472  CL79 HEK-293T C246(1.04); C51(1.04)  LDD1675  [6]
 LDCM0473  CL8 HEK-293T C246(1.71)  LDD1676  [6]
 LDCM0474  CL80 HEK-293T C246(0.88)  LDD1677  [6]
 LDCM0475  CL81 HEK-293T C51(1.11)  LDD1678  [6]
 LDCM0476  CL82 HEK-293T C51(1.10)  LDD1679  [6]
 LDCM0478  CL84 HEK-293T C51(0.76)  LDD1681  [6]
 LDCM0481  CL87 HEK-293T C246(1.25)  LDD1684  [6]
 LDCM0484  CL9 HEK-293T C51(1.18)  LDD1687  [6]
 LDCM0485  CL90 HEK-293T C246(1.02)  LDD1688  [6]
 LDCM0486  CL91 HEK-293T C246(1.13); C51(1.03)  LDD1689  [6]
 LDCM0487  CL92 HEK-293T C246(0.92)  LDD1690  [6]
 LDCM0488  CL93 HEK-293T C51(1.09)  LDD1691  [6]
 LDCM0489  CL94 HEK-293T C51(1.03)  LDD1692  [6]
 LDCM0491  CL96 HEK-293T C51(0.80)  LDD1694  [6]
 LDCM0494  CL99 HEK-293T C246(0.90)  LDD1697  [6]
 LDCM0495  E2913 HEK-293T C246(0.97)  LDD1698  [6]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C953(3.36); C74(0.61)  LDD1702  [3]
 LDCM0468  Fragment33 HEK-293T C246(1.12)  LDD1671  [6]
 LDCM0022  KB02 T cell C651(6.10)  LDD1703  [5]
 LDCM0023  KB03 MDA-MB-231 C953(3.88)  LDD1701  [3]
 LDCM0024  KB05 MKN45 C51(3.42); C246(1.95)  LDD3332  [2]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C246(0.88)  LDD2137  [3]
 LDCM0131  RA190 MM1.R 2.61  LDD0300  [4]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.
5 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402