Details of the Target

General Information of Target

Target ID LDTP07305
Target Name Rap1 GTPase-activating protein 2 (RAP1GAP2)
Gene Name RAP1GAP2
Gene ID 23108
Synonyms
GARNL4; KIAA1039; RAP1GA2; Rap1 GTPase-activating protein 2; Rap1GAP2; GTPase-activating Rap/Ran-GAP domain-like protein 4
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MFGRKRSVSFGGFGWIDKTMLASLKVKKQELANSSDATLPDRPLSPPLTAPPTMKSSEFF
EMLEKMQGIKLEEQKPGPQKNKDDYIPYPSIDEVVEKGGPYPQVILPQFGGYWIEDPENV
GTPTSLGSSICEEEEEDNLSPNTFGYKLECKGEARAYRRHFLGKDHLNFYCTGSSLGNLI
LSVKCEEAEGIEYLRVILRSKLKTVHERIPLAGLSKLPSVPQIAKAFCDDAVGLRFNPVL
YPKASQMIVSYDEHEVNNTFKFGVIYQKARQTLEEELFGNNEESPAFKEFLDLLGDTITL
QDFKGFRGGLDVTHGQTGVESVYTTFRDREIMFHVSTKLPFTDGDAQQLQRKRHIGNDIV
AIIFQEENTPFVPDMIASNFLHAYIVVQVETPGTETPSYKVSVTAREDVPTFGPPLPSPP
VFQKGPEFREFLLTKLTNAENACCKSDKFAKLEDRTRAALLDNLHDELHAHTQAMLGLGP
EEDKFENGGHGGFLESFKRAIRVRSHSMETMVGGQKKSHSGGIPGSLSGGISHNSMEVTK
TTFSPPVVAATVKNQSRSPIKRRSGLFPRLHTGSEGQGDSRARCDSTSSTPKTPDGGHSS
QEIKSETSSNPSSPEICPNKEKPFMKLKENGRAISRSSSSTSSVSSTAGEGEAMEEGDSG
GSQPSTTSPFKQEVFVYSPSPSSESPSLGAAATPIIMSRSPTDAKSRNSPRSNLKFRFDK
LSHASSGAGH
Target Bioclass
Other
Subcellular location
Cytoplasm
Function GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.
Uniprot ID
Q684P5
Ensemble ID
ENST00000254695.13
HGNC ID
HGNC:29176

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CHL1 SNV: p.E509K .
COLO320 SNV: p.A37E; p.T38I .
COLO792 SNV: p.G77R .
DU145 SNV: p.A22V .
HEC1 SNV: p.P340S .
JM1 SNV: p.G597R .
KARPAS422 SNV: p.T124A .
NCIH1703 Deletion: p.E650RfsTer48 .
NCIH716 SNV: p.D41A; p.F567S .
TFK1 SNV: p.G110W .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
BTD
 Probe Info  		C584(1.09)  LDD2096  [1]
NAIA_5
 Probe Info  		C584(0.78)  LDD2227  [2]
DBIA
 Probe Info  		C228(8.49)  LDD0209  [3]
m-APA
 Probe Info  		N.A.  LDD2231  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		C584(0.00); C228(0.00)  LDD0038  [5]
IA-alkyne
 Probe Info  		C228(0.00); C584(0.00)  LDD0036  [5]
Lodoacetamide azide
 Probe Info  		C228(0.00); C171(0.00); C584(0.00); C185(0.00)  LDD0037  [5]
IPM
 Probe Info  		C617(0.00); C584(0.00)  LDD2156  [6]
TFBX
 Probe Info  		N.A.  LDD0148  [7]
VSF
 Probe Info  		N.A.  LDD0007  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C584(4.30)  LDD0371  [9]
 LDCM0263  AC143 HCT 116 C443(0.76); C444(0.76)  LDD0580  [10]
 LDCM0264  AC144 HCT 116 C443(0.73); C444(0.82)  LDD0581  [10]
 LDCM0265  AC145 HCT 116 C444(0.77); C443(0.85)  LDD0582  [10]
 LDCM0266  AC146 HCT 116 C444(0.77); C443(0.80)  LDD0583  [10]
 LDCM0267  AC147 HCT 116 C443(1.04); C444(1.06)  LDD0584  [10]
 LDCM0268  AC148 HCT 116 C443(0.73); C444(0.80)  LDD0585  [10]
 LDCM0269  AC149 HCT 116 C443(0.67); C444(0.84)  LDD0586  [10]
 LDCM0271  AC150 HCT 116 C444(0.76); C443(0.86)  LDD0588  [10]
 LDCM0272  AC151 HCT 116 C444(0.67); C443(0.91)  LDD0589  [10]
 LDCM0273  AC152 HCT 116 C443(0.74); C444(0.79)  LDD0590  [10]
 LDCM0274  AC153 HCT 116 C444(0.41); C443(0.61)  LDD0591  [10]
 LDCM0621  AC154 HCT 116 C443(1.02); C444(0.65)  LDD2158  [10]
 LDCM0622  AC155 HCT 116 C443(0.68); C444(0.56)  LDD2159  [10]
 LDCM0623  AC156 HCT 116 C443(0.97); C444(0.75)  LDD2160  [10]
 LDCM0624  AC157 HCT 116 C443(0.73); C444(0.73)  LDD2161  [10]
 LDCM0320  AC57 HCT 116 C443(1.04); C444(1.04)  LDD0637  [10]
 LDCM0321  AC58 HCT 116 C443(0.90); C444(0.90)  LDD0638  [10]
 LDCM0322  AC59 HCT 116 C443(0.98); C444(0.98)  LDD0639  [10]
 LDCM0324  AC60 HCT 116 C443(0.93); C444(0.93)  LDD0641  [10]
 LDCM0325  AC61 HCT 116 C443(0.86); C444(0.86)  LDD0642  [10]
 LDCM0326  AC62 HCT 116 C443(0.83); C444(0.83)  LDD0643  [10]
 LDCM0327  AC63 HCT 116 C443(0.93); C444(0.93)  LDD0644  [10]
 LDCM0328  AC64 HCT 116 C443(0.89); C444(0.89)  LDD0645  [10]
 LDCM0329  AC65 HCT 116 C443(0.89); C444(0.89)  LDD0646  [10]
 LDCM0330  AC66 HCT 116 C443(0.96); C444(0.96)  LDD0647  [10]
 LDCM0331  AC67 HCT 116 C443(0.87); C444(0.87)  LDD0648  [10]
 LDCM0632  CL-Sc Hep-G2 C584(0.78)  LDD2227  [2]
 LDCM0367  CL1 HCT 116 C443(1.19); C444(1.19)  LDD0684  [10]
 LDCM0368  CL10 HCT 116 C443(0.92); C444(0.92)  LDD0685  [10]
 LDCM0372  CL103 HEK-293T C584(0.94)  LDD1576  [11]
 LDCM0376  CL107 HEK-293T C584(1.35)  LDD1580  [11]
 LDCM0379  CL11 HCT 116 C443(0.95); C444(0.95)  LDD0696  [10]
 LDCM0381  CL111 HEK-293T C584(1.22)  LDD1585  [11]
 LDCM0385  CL115 HEK-293T C584(1.36)  LDD1589  [11]
 LDCM0389  CL119 HEK-293T C584(1.21)  LDD1593  [11]
 LDCM0390  CL12 HCT 116 C443(0.97); C444(0.97)  LDD0707  [10]
 LDCM0394  CL123 HEK-293T C584(1.20)  LDD1598  [11]
 LDCM0396  CL125 HCT 116 C443(1.07); C444(1.07)  LDD0713  [10]
 LDCM0397  CL126 HCT 116 C443(0.94); C444(0.94)  LDD0714  [10]
 LDCM0398  CL127 HCT 116 C443(0.88); C444(0.88)  LDD0715  [10]
 LDCM0399  CL128 HCT 116 C443(0.92); C444(0.92)  LDD0716  [10]
 LDCM0400  CL13 HCT 116 C443(0.89); C444(0.89)  LDD0717  [10]
 LDCM0401  CL14 HCT 116 C443(1.05); C444(1.05)  LDD0718  [10]
 LDCM0402  CL15 HCT 116 C443(1.04); C444(1.04)  LDD0719  [10]
 LDCM0407  CL2 HCT 116 C443(1.21); C444(1.21)  LDD0724  [10]
 LDCM0415  CL27 HEK-293T C584(1.36)  LDD1619  [11]
 LDCM0418  CL3 HCT 116 C443(0.92); C444(0.92)  LDD0735  [10]
 LDCM0428  CL39 HEK-293T C584(1.44)  LDD1632  [11]
 LDCM0429  CL4 HCT 116 C443(1.00); C444(1.00)  LDD0746  [10]
 LDCM0440  CL5 HCT 116 C443(0.98); C444(0.98)  LDD0757  [10]
 LDCM0451  CL6 HCT 116 C443(1.21); C444(1.21)  LDD0768  [10]
 LDCM0453  CL61 HCT 116 C443(0.96); C444(0.96)  LDD0770  [10]
 LDCM0454  CL62 HCT 116 C443(0.98); C444(0.98)  LDD0771  [10]
 LDCM0455  CL63 HCT 116 C443(0.95); C444(0.95)  LDD0772  [10]
 LDCM0456  CL64 HCT 116 C443(0.84); C444(0.84)  LDD0773  [10]
 LDCM0457  CL65 HCT 116 C443(0.94); C444(0.94)  LDD0774  [10]
 LDCM0458  CL66 HCT 116 C443(0.83); C444(0.83)  LDD0775  [10]
 LDCM0459  CL67 HCT 116 C443(0.82); C444(0.82)  LDD0776  [10]
 LDCM0460  CL68 HCT 116 C443(0.91); C444(0.91)  LDD0777  [10]
 LDCM0461  CL69 HCT 116 C443(1.21); C444(1.21)  LDD0778  [10]
 LDCM0462  CL7 HCT 116 C443(0.99); C444(0.99)  LDD0779  [10]
 LDCM0463  CL70 HCT 116 C443(0.97); C444(0.97)  LDD0780  [10]
 LDCM0464  CL71 HCT 116 C443(0.99); C444(0.99)  LDD0781  [10]
 LDCM0465  CL72 HCT 116 C443(0.99); C444(0.99)  LDD0782  [10]
 LDCM0466  CL73 HCT 116 C443(0.92); C444(0.92)  LDD0783  [10]
 LDCM0467  CL74 HCT 116 C443(0.90); C444(0.90)  LDD0784  [10]
 LDCM0469  CL76 HCT 116 C443(0.86); C444(0.86)  LDD0786  [10]
 LDCM0470  CL77 HCT 116 C443(1.08); C444(1.08)  LDD0787  [10]
 LDCM0471  CL78 HCT 116 C443(0.92); C444(0.92)  LDD0788  [10]
 LDCM0472  CL79 HCT 116 C443(0.99); C444(0.99)  LDD0789  [10]
 LDCM0473  CL8 HCT 116 C443(1.01); C444(1.01)  LDD0790  [10]
 LDCM0474  CL80 HCT 116 C443(1.08); C444(1.08)  LDD0791  [10]
 LDCM0475  CL81 HCT 116 C443(1.07); C444(1.07)  LDD0792  [10]
 LDCM0476  CL82 HCT 116 C443(0.95); C444(0.95)  LDD0793  [10]
 LDCM0477  CL83 HCT 116 C443(0.98); C444(0.98)  LDD0794  [10]
 LDCM0478  CL84 HCT 116 C443(1.19); C444(1.19)  LDD0795  [10]
 LDCM0479  CL85 HCT 116 C443(0.95); C444(0.95)  LDD0796  [10]
 LDCM0480  CL86 HCT 116 C443(0.99); C444(0.99)  LDD0797  [10]
 LDCM0481  CL87 HCT 116 C443(1.25); C444(1.25)  LDD0798  [10]
 LDCM0482  CL88 HCT 116 C443(1.17); C444(1.17)  LDD0799  [10]
 LDCM0483  CL89 HCT 116 C443(0.99); C444(0.99)  LDD0800  [10]
 LDCM0484  CL9 HCT 116 C443(1.09); C444(1.09)  LDD0801  [10]
 LDCM0485  CL90 HCT 116 C443(1.51); C444(1.51)  LDD0802  [10]
 LDCM0494  CL99 HEK-293T C584(1.07)  LDD1697  [11]
 LDCM0495  E2913 HEK-293T C584(1.54)  LDD1698  [11]
 LDCM0625  F8 Ramos C228(1.42)  LDD2187  [12]
 LDCM0572  Fragment10 Ramos C228(1.18)  LDD2189  [12]
 LDCM0573  Fragment11 Ramos C228(1.09)  LDD2190  [12]
 LDCM0574  Fragment12 Ramos C228(1.15)  LDD2191  [12]
 LDCM0575  Fragment13 Ramos C228(0.82)  LDD2192  [12]
 LDCM0576  Fragment14 Ramos C228(0.61)  LDD2193  [12]
 LDCM0579  Fragment20 Ramos C228(0.92)  LDD2194  [12]
 LDCM0586  Fragment28 Ramos C228(0.75)  LDD2198  [12]
 LDCM0588  Fragment30 Ramos C228(0.78)  LDD2199  [12]
 LDCM0589  Fragment31 Ramos C228(0.96)  LDD2200  [12]
 LDCM0590  Fragment32 Ramos C228(1.16)  LDD2201  [12]
 LDCM0468  Fragment33 HCT 116 C443(0.99); C444(0.99)  LDD0785  [10]
 LDCM0596  Fragment38 Ramos C228(0.70)  LDD2203  [12]
 LDCM0566  Fragment4 Ramos C228(0.97)  LDD2184  [12]
 LDCM0610  Fragment52 Ramos C228(0.70)  LDD2204  [12]
 LDCM0614  Fragment56 Ramos C228(0.77)  LDD2205  [12]
 LDCM0569  Fragment7 Ramos C228(0.85)  LDD2186  [12]
 LDCM0571  Fragment9 Ramos C228(1.56)  LDD2188  [12]
 LDCM0022  KB02 Ramos C228(1.44)  LDD2182  [12]
 LDCM0023  KB03 Jurkat C228(8.49)  LDD0209  [3]
 LDCM0024  KB05 SiSo C228(7.81)  LDD3421  [13]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C584(1.09)  LDD2096  [1]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C584(1.05)  LDD2118  [1]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C584(1.03)  LDD2122  [1]

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
2 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
3 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
4 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
7 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
8 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
9 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
10 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
11 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
12 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
13 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840