Details of the Target

General Information of Target

Target ID LDTP07210
Target Name G kinase-anchoring protein 1 (GKAP1)
Gene Name GKAP1
Gene ID 80318
Synonyms
GKAP42; G kinase-anchoring protein 1; cGMP-dependent protein kinase-anchoring protein of 42 kDa
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MASAVLSSVPTTASRFALLQVDSGSGSDSEPGKGKGRNTGKSQTLGSKSTTNEKKREKRR
KKKEQQQSEANELRNLAFKKIPQKSSHAVCNAQHDLPLSNPVQKDSREENWQEWRQRDEQ
LTSEMFEADLEKALLLSKLEYEEHKKEYEDAENTSTQSKVMNKKDKRKNHQGKDRPLTVS
LKDFHSEDHISKKTEELSSSQTLSHDGGFFNRLEDDVHKILIREKRREQLTEYNGTDNCT
AHEHNQEVVLKDGRIERLKLELERKDAEIQKLKNVITQWEAKYKEVKARNAQLLKMLQEG
EMKDKAEILLQVDESQSIKNELTIQVTSLHAALEQERSKVKVLQAELAKYQGGRKGKRNS
ESDQCR
Target Bioclass
Other
Family
GKAP1 family
Subcellular location
Golgi apparatus
Function
Regulates insulin-dependent IRS1 tyrosine phosphorylation in adipocytes by modulating the availability of IRS1 to IR tyrosine kinase. Its association with IRS1 is required for insulin-induced translocation of SLC2A4 to the cell membrane. Involved in TNF-induced impairment of insulin-dependent IRS1 tyrosine phosphorylation.
Uniprot ID
Q5VSY0
Ensemble ID
ENST00000376365.7
HGNC ID
HGNC:17496

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 SNV: p.R354Ter .
KMCH1 SNV: p.D266E .
LNCaP clone FGC SNV: p.L18M .
LOXIMVI SNV: p.R37Q .
MFE319 SNV: p.A133T .
NALM6 SNV: p.T154I .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C365(1.65)  LDD3440  [1]
AHL-Pu-1
 Probe Info  		C90(2.46)  LDD0168  [2]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [3]
IA-alkyne
 Probe Info  		N.A.  LDD2241  [3]
IPM
 Probe Info  		N.A.  LDD2156  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C90(2.46)  LDD0168  [2]
 LDCM0259  AC14 HEK-293T C90(1.14); C365(1.10)  LDD1512  [5]
 LDCM0281  AC21 HEK-293T C239(0.86)  LDD1520  [5]
 LDCM0282  AC22 HEK-293T C90(0.94); C365(1.16)  LDD1521  [5]
 LDCM0284  AC24 HEK-293T C90(0.98)  LDD1523  [5]
 LDCM0289  AC29 HEK-293T C239(1.04)  LDD1528  [5]
 LDCM0291  AC30 HEK-293T C90(1.01); C365(1.11)  LDD1530  [5]
 LDCM0293  AC32 HEK-293T C90(1.02)  LDD1532  [5]
 LDCM0298  AC37 HEK-293T C239(0.99)  LDD1537  [5]
 LDCM0299  AC38 HEK-293T C90(1.12); C365(1.21)  LDD1538  [5]
 LDCM0302  AC40 HEK-293T C90(1.15)  LDD1541  [5]
 LDCM0307  AC45 HEK-293T C239(0.98)  LDD1546  [5]
 LDCM0308  AC46 HEK-293T C90(1.04); C365(1.19)  LDD1547  [5]
 LDCM0310  AC48 HEK-293T C90(0.94)  LDD1549  [5]
 LDCM0312  AC5 HEK-293T C239(1.03)  LDD1551  [5]
 LDCM0316  AC53 HEK-293T C239(1.17)  LDD1555  [5]
 LDCM0317  AC54 HEK-293T C90(1.01); C365(1.16)  LDD1556  [5]
 LDCM0319  AC56 HEK-293T C90(1.08)  LDD1558  [5]
 LDCM0323  AC6 HEK-293T C90(0.91); C365(1.11)  LDD1562  [5]
 LDCM0325  AC61 HEK-293T C239(1.14)  LDD1564  [5]
 LDCM0326  AC62 HEK-293T C90(0.92); C365(0.98)  LDD1565  [5]
 LDCM0328  AC64 HEK-293T C90(0.98)  LDD1567  [5]
 LDCM0345  AC8 HEK-293T C90(1.11)  LDD1569  [5]
 LDCM0248  AKOS034007472 HEK-293T C239(0.97)  LDD1511  [5]
 LDCM0275  AKOS034007705 HEK-293T C90(1.13)  LDD1514  [5]
 LDCM0367  CL1 HEK-293T C239(1.06)  LDD1571  [5]
 LDCM0368  CL10 HEK-293T C90(1.16); C365(0.91)  LDD1572  [5]
 LDCM0369  CL100 HEK-293T C239(1.21); C90(1.09)  LDD1573  [5]
 LDCM0370  CL101 HEK-293T C239(1.02)  LDD1574  [5]
 LDCM0371  CL102 HEK-293T C239(0.90)  LDD1575  [5]
 LDCM0372  CL103 HEK-293T C239(0.97)  LDD1576  [5]
 LDCM0373  CL104 HEK-293T C239(0.93); C90(0.82)  LDD1577  [5]
 LDCM0374  CL105 HEK-293T C239(0.99)  LDD1578  [5]
 LDCM0375  CL106 HEK-293T C239(0.84)  LDD1579  [5]
 LDCM0376  CL107 HEK-293T C239(0.83)  LDD1580  [5]
 LDCM0377  CL108 HEK-293T C239(0.94); C90(0.87)  LDD1581  [5]
 LDCM0378  CL109 HEK-293T C239(0.86)  LDD1582  [5]
 LDCM0380  CL110 HEK-293T C239(0.79)  LDD1584  [5]
 LDCM0381  CL111 HEK-293T C239(0.91)  LDD1585  [5]
 LDCM0382  CL112 HEK-293T C239(1.34); C90(0.89)  LDD1586  [5]
 LDCM0383  CL113 HEK-293T C239(1.24)  LDD1587  [5]
 LDCM0384  CL114 HEK-293T C239(0.99)  LDD1588  [5]
 LDCM0385  CL115 HEK-293T C239(0.84)  LDD1589  [5]
 LDCM0386  CL116 HEK-293T C239(0.94); C90(1.14)  LDD1590  [5]
 LDCM0387  CL117 HEK-293T C239(1.03)  LDD1591  [5]
 LDCM0388  CL118 HEK-293T C239(1.17)  LDD1592  [5]
 LDCM0389  CL119 HEK-293T C239(0.82)  LDD1593  [5]
 LDCM0390  CL12 HEK-293T C90(1.16)  LDD1594  [5]
 LDCM0391  CL120 HEK-293T C239(0.96); C90(0.99)  LDD1595  [5]
 LDCM0392  CL121 HEK-293T C239(0.90)  LDD1596  [5]
 LDCM0393  CL122 HEK-293T C239(0.78)  LDD1597  [5]
 LDCM0394  CL123 HEK-293T C239(0.82)  LDD1598  [5]
 LDCM0395  CL124 HEK-293T C239(1.18); C90(0.88)  LDD1599  [5]
 LDCM0396  CL125 HEK-293T C239(1.05)  LDD1600  [5]
 LDCM0397  CL126 HEK-293T C239(1.07)  LDD1601  [5]
 LDCM0398  CL127 HEK-293T C239(0.99)  LDD1602  [5]
 LDCM0399  CL128 HEK-293T C239(1.03); C90(0.92)  LDD1603  [5]
 LDCM0400  CL13 HEK-293T C239(1.36)  LDD1604  [5]
 LDCM0401  CL14 HEK-293T C239(1.14)  LDD1605  [5]
 LDCM0402  CL15 HEK-293T C239(0.82)  LDD1606  [5]
 LDCM0403  CL16 HEK-293T C239(0.99); C90(0.99)  LDD1607  [5]
 LDCM0407  CL2 HEK-293T C239(0.75)  LDD1611  [5]
 LDCM0409  CL21 HEK-293T C239(1.32)  LDD1613  [5]
 LDCM0410  CL22 HEK-293T C90(1.15); C365(0.87)  LDD1614  [5]
 LDCM0412  CL24 HEK-293T C90(1.17)  LDD1616  [5]
 LDCM0413  CL25 HEK-293T C239(1.05)  LDD1617  [5]
 LDCM0414  CL26 HEK-293T C239(0.70)  LDD1618  [5]
 LDCM0415  CL27 HEK-293T C239(0.93)  LDD1619  [5]
 LDCM0416  CL28 HEK-293T C239(1.08); C90(0.87)  LDD1620  [5]
 LDCM0418  CL3 HEK-293T C239(0.94)  LDD1622  [5]
 LDCM0422  CL33 HEK-293T C239(0.91)  LDD1626  [5]
 LDCM0423  CL34 HEK-293T C90(1.19); C365(0.94)  LDD1627  [5]
 LDCM0425  CL36 HEK-293T C90(1.10)  LDD1629  [5]
 LDCM0426  CL37 HEK-293T C239(0.96)  LDD1630  [5]
 LDCM0428  CL39 HEK-293T C239(0.78)  LDD1632  [5]
 LDCM0429  CL4 HEK-293T C239(1.00); C90(0.92)  LDD1633  [5]
 LDCM0430  CL40 HEK-293T C239(1.16); C90(0.89)  LDD1634  [5]
 LDCM0435  CL45 HEK-293T C239(1.37)  LDD1639  [5]
 LDCM0436  CL46 HEK-293T C90(1.21); C365(1.00)  LDD1640  [5]
 LDCM0438  CL48 HEK-293T C90(0.96)  LDD1642  [5]
 LDCM0439  CL49 HEK-293T C239(0.92)  LDD1643  [5]
 LDCM0441  CL50 HEK-293T C239(0.76)  LDD1645  [5]
 LDCM0443  CL52 HEK-293T C239(1.06); C90(1.24)  LDD1646  [5]
 LDCM0448  CL57 HEK-293T C239(1.13)  LDD1651  [5]
 LDCM0449  CL58 HEK-293T C90(1.12); C365(0.96)  LDD1652  [5]
 LDCM0452  CL60 HEK-293T C90(1.05)  LDD1655  [5]
 LDCM0453  CL61 HEK-293T C239(0.85)  LDD1656  [5]
 LDCM0454  CL62 HEK-293T C239(0.83)  LDD1657  [5]
 LDCM0455  CL63 HEK-293T C239(0.98)  LDD1658  [5]
 LDCM0456  CL64 HEK-293T C239(1.04); C90(0.90)  LDD1659  [5]
 LDCM0461  CL69 HEK-293T C239(1.17)  LDD1664  [5]
 LDCM0463  CL70 HEK-293T C90(1.42); C365(0.99)  LDD1666  [5]
 LDCM0465  CL72 HEK-293T C90(1.00)  LDD1668  [5]
 LDCM0466  CL73 HEK-293T C239(0.97)  LDD1669  [5]
 LDCM0467  CL74 HEK-293T C239(0.69)  LDD1670  [5]
 LDCM0469  CL76 HEK-293T C239(1.06); C90(0.91)  LDD1672  [5]
 LDCM0475  CL81 HEK-293T C239(1.30)  LDD1678  [5]
 LDCM0476  CL82 HEK-293T C90(1.12); C365(1.00)  LDD1679  [5]
 LDCM0478  CL84 HEK-293T C90(1.07)  LDD1681  [5]
 LDCM0479  CL85 HEK-293T C239(0.91)  LDD1682  [5]
 LDCM0480  CL86 HEK-293T C239(0.81)  LDD1683  [5]
 LDCM0481  CL87 HEK-293T C239(0.82)  LDD1684  [5]
 LDCM0482  CL88 HEK-293T C239(0.89); C90(0.90)  LDD1685  [5]
 LDCM0484  CL9 HEK-293T C239(1.30)  LDD1687  [5]
 LDCM0488  CL93 HEK-293T C239(1.10)  LDD1691  [5]
 LDCM0489  CL94 HEK-293T C90(1.23); C365(1.04)  LDD1692  [5]
 LDCM0491  CL96 HEK-293T C90(0.89)  LDD1694  [5]
 LDCM0492  CL97 HEK-293T C239(0.92)  LDD1695  [5]
 LDCM0493  CL98 HEK-293T C239(1.02)  LDD1696  [5]
 LDCM0494  CL99 HEK-293T C239(0.97)  LDD1697  [5]
 LDCM0495  E2913 HEK-293T C239(0.73)  LDD1698  [5]
 LDCM0468  Fragment33 HEK-293T C239(0.76)  LDD1671  [5]
 LDCM0427  Fragment51 HEK-293T C239(1.26)  LDD1631  [5]
 LDCM0022  KB02 HEK-293T C90(1.17)  LDD1492  [5]
 LDCM0023  KB03 HEK-293T C90(1.07)  LDD1497  [5]
 LDCM0024  KB05 SNU-245 C365(1.65)  LDD3440  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Pyridoxal phosphate phosphatase PHOSPHO2 (PHOSPHO2) PHOSPHO family Q8TCD6
Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1 (HDDC3) MESH1 family Q8N4P3
Histone acetyltransferase KAT5 (KAT5) MYST (SAS/MOZ) family Q92993
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Syntenin-1 (SDCBP) . O00560
Other
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
G kinase-anchoring protein 1 (GKAP1) GKAP1 family Q5VSY0
Microfibrillar-associated protein 1 (MFAP1) MFAP1 family P55081
KN motif and ankyrin repeat domain-containing protein 2 (KANK2) . Q63ZY3
Lethal(3)malignant brain tumor-like protein 2 (L3MBTL2) . Q969R5
Multiple myeloma tumor-associated protein 2 (MMTAG2) . Q9BU76
Zinc finger CCHC domain-containing protein 10 (ZCCHC10) . Q8TBK6

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402