Details of the Target

General Information of Target

Target ID LDTP06801
Target Name Protein PTHB1 (BBS9)
Gene Name BBS9
Gene ID 27241
Synonyms
PTHB1; Protein PTHB1; Bardet-Biedl syndrome 9 protein; Parathyroid hormone-responsive B1 gene protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSLFKARDWWSTILGDKEEFDQGCLCLANVDNSGNGQDKIIVGSFMGYLRIFSPHPAKTG
DGAQAEDLLLEVDLRDPVLQVEVGKFVSGTEMLHLAVLHSRKLCVYSVSGTLGNVEHGNQ
CQMKLMYEHNLQRTACNMTYGSFGGVKGRDLICIQSMDGMLMVFEQESYAFGRFLPGFLL
PGPLAYSSRTDSFLTVSSCQQVESYKYQVLAFATDADKRQETEQQKLGSGKRLVVDWTLN
IGEQALDICIVSFNQSASSVFVLGERNFFCLKDNGQIRFMKKLDWSPSCFLPYCSVSEGT
INTLIGNHNNMLHIYQDVTLKWATQLPHIPVAVRVGCLHDLKGVIVTLSDDGHLQCSYLG
TDPSLFQAPNVQSRELNYDELDVEMKELQKIIKDVNKSQGVWPMTEREDDLNVSVVVSPN
FDSVSQATDVEVGTDLVPSVTVKVTLQNRVILQKAKLSVYVQPPLELTCDQFTFEFMTPD
LTRTVSFSVYLKRSYTPSELEGNAVVSYSRPTDRNPDGIPRVIQCKFRLPLKLICLPGQP
SKTASHKITIDTNKSPVSLLSLFPGFASQSDDDQVNVMGFHFLGGARITVLASKTSQRYR
IQSEQFEDLWLITNELILRLQEYFEKQGVKDFACSFSGSIPLQEYFELIDHHFELRINGE
KLEELLSERAVQFRAIQRRLLARFKDKTPAPLQHLDTLLDGTYKQVIALADAVEENQGNL
FQSFTRLKSATHLVILLIALWQKLSADQVAILEAAFLPLQEDTQELGWEETVDAAISHLL
KTCLSKSSKEQALNLNSQLNIPKDTSQLKKHITLLCDRLSKGGRLCLSTDAAAPQTMVMP
GGCTTIPESDLEERSVEQDSTELFTNHRHLTAETPRPEVSPLQGVSE
Target Bioclass
Transporter and channel
Subcellular location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Function
The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. Required for proper BBSome complex assembly and its ciliary localization.
Uniprot ID
Q3SYG4
Ensemble ID
ENST00000242067.11
HGNC ID
HGNC:30000

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL27 SNV: p.S458Ter .
CCLP1 SNV: p.S486N .
CHL1 SNV: p.W9Ter .
HUH28 SNV: p.Q64E DBIA    Probe Info 
LS180 SNV: p.T549S .
MKN45 SNV: p.Q372Ter .
SW403 SNV: p.Y599F .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C337(1.53)  LDD3314  [1]
NAIA_4
 Probe Info  		N.A.  LDD2226  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0259  AC14 HEK-293T C337(0.95); C634(1.01)  LDD1512  [3]
 LDCM0270  AC15 HEK-293T C337(0.90); C634(0.97)  LDD1513  [3]
 LDCM0281  AC21 HEK-293T C337(0.96)  LDD1520  [3]
 LDCM0282  AC22 HEK-293T C337(0.93); C634(1.06)  LDD1521  [3]
 LDCM0283  AC23 HEK-293T C337(0.96); C634(1.10)  LDD1522  [3]
 LDCM0284  AC24 HEK-293T C634(0.98)  LDD1523  [3]
 LDCM0289  AC29 HEK-293T C337(0.98)  LDD1528  [3]
 LDCM0291  AC30 HEK-293T C337(0.95); C634(0.95)  LDD1530  [3]
 LDCM0292  AC31 HEK-293T C337(0.91); C634(1.04)  LDD1531  [3]
 LDCM0293  AC32 HEK-293T C634(1.13)  LDD1532  [3]
 LDCM0298  AC37 HEK-293T C337(1.04)  LDD1537  [3]
 LDCM0299  AC38 HEK-293T C337(0.92); C634(1.23)  LDD1538  [3]
 LDCM0300  AC39 HEK-293T C337(0.87); C634(1.11)  LDD1539  [3]
 LDCM0302  AC40 HEK-293T C634(0.96)  LDD1541  [3]
 LDCM0307  AC45 HEK-293T C337(1.02)  LDD1546  [3]
 LDCM0308  AC46 HEK-293T C337(0.88); C634(1.21)  LDD1547  [3]
 LDCM0309  AC47 HEK-293T C337(1.04); C634(1.15)  LDD1548  [3]
 LDCM0310  AC48 HEK-293T C634(0.95)  LDD1549  [3]
 LDCM0312  AC5 HEK-293T C337(1.16)  LDD1551  [3]
 LDCM0316  AC53 HEK-293T C337(1.02)  LDD1555  [3]
 LDCM0317  AC54 HEK-293T C337(1.01); C634(1.23)  LDD1556  [3]
 LDCM0318  AC55 HEK-293T C337(0.89); C634(1.11)  LDD1557  [3]
 LDCM0319  AC56 HEK-293T C634(1.10)  LDD1558  [3]
 LDCM0323  AC6 HEK-293T C337(0.95); C634(1.08)  LDD1562  [3]
 LDCM0325  AC61 HEK-293T C337(1.00)  LDD1564  [3]
 LDCM0326  AC62 HEK-293T C337(0.99); C634(1.20)  LDD1565  [3]
 LDCM0327  AC63 HEK-293T C337(0.92); C634(1.06)  LDD1566  [3]
 LDCM0328  AC64 HEK-293T C634(1.03)  LDD1567  [3]
 LDCM0334  AC7 HEK-293T C337(1.01); C634(0.86)  LDD1568  [3]
 LDCM0345  AC8 HEK-293T C634(1.06)  LDD1569  [3]
 LDCM0248  AKOS034007472 HEK-293T C337(1.08)  LDD1511  [3]
 LDCM0275  AKOS034007705 HEK-293T C634(0.93)  LDD1514  [3]
 LDCM0367  CL1 HEK-293T C337(0.99)  LDD1571  [3]
 LDCM0368  CL10 HEK-293T C337(0.81); C634(0.92)  LDD1572  [3]
 LDCM0369  CL100 HEK-293T C337(0.94)  LDD1573  [3]
 LDCM0370  CL101 HEK-293T C337(1.00)  LDD1574  [3]
 LDCM0371  CL102 HEK-293T C634(1.12); C535(1.30)  LDD1575  [3]
 LDCM0372  CL103 HEK-293T C535(1.05)  LDD1576  [3]
 LDCM0373  CL104 HEK-293T C337(0.96)  LDD1577  [3]
 LDCM0374  CL105 HEK-293T C337(1.02)  LDD1578  [3]
 LDCM0375  CL106 HEK-293T C634(1.36); C535(1.13)  LDD1579  [3]
 LDCM0376  CL107 HEK-293T C535(1.02)  LDD1580  [3]
 LDCM0377  CL108 HEK-293T C337(0.97)  LDD1581  [3]
 LDCM0378  CL109 HEK-293T C337(0.95)  LDD1582  [3]
 LDCM0379  CL11 HEK-293T C337(1.04); C634(0.91)  LDD1583  [3]
 LDCM0380  CL110 HEK-293T C634(1.13); C535(1.14)  LDD1584  [3]
 LDCM0381  CL111 HEK-293T C535(1.28)  LDD1585  [3]
 LDCM0382  CL112 HEK-293T C337(0.90)  LDD1586  [3]
 LDCM0383  CL113 HEK-293T C337(0.93)  LDD1587  [3]
 LDCM0384  CL114 HEK-293T C634(0.84); C535(1.16)  LDD1588  [3]
 LDCM0385  CL115 HEK-293T C535(1.11)  LDD1589  [3]
 LDCM0386  CL116 HEK-293T C337(0.97)  LDD1590  [3]
 LDCM0387  CL117 HEK-293T C337(0.91)  LDD1591  [3]
 LDCM0388  CL118 HEK-293T C634(1.15); C535(1.29)  LDD1592  [3]
 LDCM0389  CL119 HEK-293T C535(1.08)  LDD1593  [3]
 LDCM0390  CL12 HEK-293T C634(0.91)  LDD1594  [3]
 LDCM0391  CL120 HEK-293T C337(1.07)  LDD1595  [3]
 LDCM0392  CL121 HEK-293T C337(0.90)  LDD1596  [3]
 LDCM0393  CL122 HEK-293T C634(1.16); C535(1.10)  LDD1597  [3]
 LDCM0394  CL123 HEK-293T C535(0.96)  LDD1598  [3]
 LDCM0395  CL124 HEK-293T C337(1.22)  LDD1599  [3]
 LDCM0396  CL125 HEK-293T C337(0.98)  LDD1600  [3]
 LDCM0397  CL126 HEK-293T C634(0.74); C535(0.97)  LDD1601  [3]
 LDCM0398  CL127 HEK-293T C535(1.15)  LDD1602  [3]
 LDCM0399  CL128 HEK-293T C337(0.96)  LDD1603  [3]
 LDCM0400  CL13 HEK-293T C337(0.96)  LDD1604  [3]
 LDCM0401  CL14 HEK-293T C634(0.77); C535(1.13)  LDD1605  [3]
 LDCM0402  CL15 HEK-293T C535(1.18)  LDD1606  [3]
 LDCM0403  CL16 HEK-293T C337(1.05)  LDD1607  [3]
 LDCM0407  CL2 HEK-293T C634(0.96); C535(1.06)  LDD1611  [3]
 LDCM0409  CL21 HEK-293T C337(1.09)  LDD1613  [3]
 LDCM0410  CL22 HEK-293T C337(1.00); C634(0.89)  LDD1614  [3]
 LDCM0411  CL23 HEK-293T C337(1.00); C634(1.12)  LDD1615  [3]
 LDCM0412  CL24 HEK-293T C634(1.40)  LDD1616  [3]
 LDCM0413  CL25 HEK-293T C337(1.00)  LDD1617  [3]
 LDCM0414  CL26 HEK-293T C634(1.07); C535(1.24)  LDD1618  [3]
 LDCM0415  CL27 HEK-293T C535(1.01)  LDD1619  [3]
 LDCM0416  CL28 HEK-293T C337(0.96)  LDD1620  [3]
 LDCM0418  CL3 HEK-293T C535(0.92)  LDD1622  [3]
 LDCM0422  CL33 HEK-293T C337(1.00)  LDD1626  [3]
 LDCM0423  CL34 HEK-293T C337(1.01); C634(1.66)  LDD1627  [3]
 LDCM0424  CL35 HEK-293T C337(1.05); C634(1.07)  LDD1628  [3]
 LDCM0425  CL36 HEK-293T C634(1.14)  LDD1629  [3]
 LDCM0426  CL37 HEK-293T C337(0.95)  LDD1630  [3]
 LDCM0428  CL39 HEK-293T C535(0.83)  LDD1632  [3]
 LDCM0429  CL4 HEK-293T C337(1.02)  LDD1633  [3]
 LDCM0430  CL40 HEK-293T C337(0.98)  LDD1634  [3]
 LDCM0435  CL45 HEK-293T C337(1.05)  LDD1639  [3]
 LDCM0436  CL46 HEK-293T C337(1.05); C634(1.05)  LDD1640  [3]
 LDCM0437  CL47 HEK-293T C337(0.99); C634(0.87)  LDD1641  [3]
 LDCM0438  CL48 HEK-293T C634(0.91)  LDD1642  [3]
 LDCM0439  CL49 HEK-293T C337(0.97)  LDD1643  [3]
 LDCM0441  CL50 HEK-293T C634(0.97); C535(1.24)  LDD1645  [3]
 LDCM0443  CL52 HEK-293T C337(0.91)  LDD1646  [3]
 LDCM0448  CL57 HEK-293T C337(1.01)  LDD1651  [3]
 LDCM0449  CL58 HEK-293T C337(0.95); C634(0.87)  LDD1652  [3]
 LDCM0450  CL59 HEK-293T C337(1.00); C634(0.95)  LDD1653  [3]
 LDCM0452  CL60 HEK-293T C634(1.08)  LDD1655  [3]
 LDCM0453  CL61 HEK-293T C337(0.88)  LDD1656  [3]
 LDCM0454  CL62 HEK-293T C634(0.86); C535(1.51)  LDD1657  [3]
 LDCM0455  CL63 HEK-293T C535(1.04)  LDD1658  [3]
 LDCM0456  CL64 HEK-293T C337(0.92)  LDD1659  [3]
 LDCM0461  CL69 HEK-293T C337(1.10)  LDD1664  [3]
 LDCM0463  CL70 HEK-293T C337(0.97); C634(0.91)  LDD1666  [3]
 LDCM0464  CL71 HEK-293T C337(0.98); C634(1.47)  LDD1667  [3]
 LDCM0465  CL72 HEK-293T C634(1.24)  LDD1668  [3]
 LDCM0466  CL73 HEK-293T C337(0.97)  LDD1669  [3]
 LDCM0467  CL74 HEK-293T C634(0.91); C535(1.14)  LDD1670  [3]
 LDCM0469  CL76 HEK-293T C337(0.97)  LDD1672  [3]
 LDCM0475  CL81 HEK-293T C337(1.11)  LDD1678  [3]
 LDCM0476  CL82 HEK-293T C337(1.01); C634(1.05)  LDD1679  [3]
 LDCM0477  CL83 HEK-293T C337(1.03); C634(0.69)  LDD1680  [3]
 LDCM0478  CL84 HEK-293T C634(0.91)  LDD1681  [3]
 LDCM0479  CL85 HEK-293T C337(0.96)  LDD1682  [3]
 LDCM0480  CL86 HEK-293T C634(0.90); C535(1.12)  LDD1683  [3]
 LDCM0481  CL87 HEK-293T C535(1.11)  LDD1684  [3]
 LDCM0482  CL88 HEK-293T C337(1.18)  LDD1685  [3]
 LDCM0484  CL9 HEK-293T C337(1.09)  LDD1687  [3]
 LDCM0488  CL93 HEK-293T C337(1.08)  LDD1691  [3]
 LDCM0489  CL94 HEK-293T C337(0.99); C634(1.25)  LDD1692  [3]
 LDCM0490  CL95 HEK-293T C337(0.82); C634(0.92)  LDD1693  [3]
 LDCM0491  CL96 HEK-293T C634(0.83)  LDD1694  [3]
 LDCM0492  CL97 HEK-293T C337(1.03)  LDD1695  [3]
 LDCM0493  CL98 HEK-293T C634(1.07); C535(1.01)  LDD1696  [3]
 LDCM0494  CL99 HEK-293T C535(0.93)  LDD1697  [3]
 LDCM0495  E2913 HEK-293T C535(1.08)  LDD1698  [3]
 LDCM0468  Fragment33 HEK-293T C535(1.17)  LDD1671  [3]
 LDCM0427  Fragment51 HEK-293T C634(0.99); C535(1.13)  LDD1631  [3]
 LDCM0022  KB02 A-172 C535(1.90)  LDD2251  [1]
 LDCM0023  KB03 A-172 C535(2.60)  LDD2668  [1]
 LDCM0024  KB05 IGR37 C337(1.53)  LDD3314  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402