Details of the Target

General Information of Target

Target ID LDTP03945
Target Name Patatin-like phospholipase domain-containing protein 4 (PNPLA4)
Gene Name PNPLA4
Gene ID 8228
Synonyms
DXS1283E; GS2; Patatin-like phospholipase domain-containing protein 4; EC 3.1.1.3; Calcium-independent phospholipase A2-eta; iPLA2-eta; EC 3.1.1.4; Protein GS2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MKHINLSFAACGFLGIYHLGAASALCRHGKKLVKDVKAFAGASAGSLVASVLLTAPEKIE
ECNQFTYKFAEEIRRQSFGAVTPGYDFMARLRSGMESILPPSAHELAQNRLHVSITNAKT
RENHLVSTFSSREDLIKVLLASSFVPIYAGLKLVEYKGQKWVDGGLTNALPILPVGRTVT
ISPFSGRLDISPQDKGQLDLYVNIAKQDIMLSLANLVRLNQALFPPSKRKMESLYQCGFD
DTVKFLLKENWFE
Target Bioclass
Enzyme
Subcellular location
Mitochondrion
Function
Has abundant triacylglycerol lipase activity. Transfers fatty acid from triglyceride to retinol, hydrolyzes retinylesters, and generates 1,3-diacylglycerol from triglycerides. Additionally possesses acylglycerol transacylase and phospholipase A2 activities.
Uniprot ID
P41247
Ensemble ID
ENST00000381042.9
HGNC ID
HGNC:24887

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT116 Insertion: p.L32TfsTer32 .
MCC26 SNV: p.R187Q .
T24 SNV: p.E72Q .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
HDSF-alk
 Probe Info  		3.22  LDD0197  [1]
FBP2
 Probe Info  		14.99  LDD0317  [2]
STPyne
 Probe Info  		K230(10.00)  LDD0277  [3]
IPM
 Probe Info  		N.A.  LDD0241  [4]
DBIA
 Probe Info  		C237(1.43)  LDD3389  [5]
TER-AC
 Probe Info  		N.A.  LDD0426  [6]
TPP-AC
 Probe Info  		N.A.  LDD0427  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 BRX211 C237(1.25)  LDD2268  [5]
 LDCM0023  KB03 BRX211 C237(2.21)  LDD2685  [5]
 LDCM0024  KB05 OZ C237(1.43)  LDD3389  [5]

References

1 Fatty Acyl Sulfonyl Fluoride as an Activity-Based Probe for Profiling Fatty Acid-Associated Proteins in Living Cells. Chembiochem. 2022 Feb 16;23(4):e202100628. doi: 10.1002/cbic.202100628. Epub 2021 Dec 30.
2 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
6 Differently Tagged Probes for Protein Profiling of Mitochondria. Chembiochem. 2019 May 2;20(9):1155-1160. doi: 10.1002/cbic.201800735. Epub 2019 Mar 26.