Details of the Target

General Information of Target

Target ID LDTP03237
Target Name Nuclear autoantigen Sp-100 (SP100)
Gene Name SP100
Gene ID 6672
Synonyms
Nuclear autoantigen Sp-100; Nuclear dot-associated Sp100 protein; Speckled 100 kDa
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAGGGGDLSTRRLNECISPVANEMNHLPAHSHDLQRMFTEDQGVDDRLLYDIVFKHFKRN
KVEISNAIKKTFPFLEGLRDRDLITNKMFEDSQDSCRNLVPVQRVVYNVLSELEKTFNLP
VLEALFSDVNMQEYPDLIHIYKGFENVIHDKLPLQESEEEEREERSGLQLSLEQGTGENS
FRSLTWPPSGSPSHAGTTPPENGLSEHPCETEQINAKRKDTTSDKDDSLGSQQTNEQCAQ
KAEPTESCEQIAVQVNNGDAGREMPCPLPCDEESPEAELHNHGIQINSCSVRLVDIKKEK
PFSNSKVECQAQARTHHNQASDIIVISSEDSEGSTDVDEPLEVFISAPRSEPVINNDNPL
ESNDEKEGQEATCSRPQIVPEPMDFRKLSTFRESFKKRVIGQDHDFSESSEEEAPAEASS
GALRSKHGEKAPMTSRSTSTWRIPSRKRRFSSSDFSDLSNGEELQETCSSSLRRGSGSQP
QEPENKKCSCVMCFPKGVPRSQEARTESSQASDMMDTMDVENNSTLEKHSGKRRKKRRHR
SKVNGLQRGRKKDRPRKHLTLNNKVQKKRWQQRGRKANTRPLKRRRKRGPRIPKDENINF
KQSELPVTCGEVKGTLYKERFKQGTSKKCIQSEDKKWFTPREFEIEGDRGASKNWKLSIR
CGGYTLKVLMENKFLPEPPSTRKKRILESHNNTLVDPCEEHKKKNPDASVKFSEFLKKCS
ETWKTIFAKEKGKFEDMAKADKAHYEREMKTYIPPKGEKKKKFKDPNAPKRPPLAFFLFC
SEYRPKIKGEHPGLSIDDVVKKLAGMWNNTAAADKQFYEKKAAKLKEKYKKDIAAYRAKG
KPNSAKKRVVKAEKSKKKKEEEEDEEDEQEEENEEDDDK
Target Bioclass
Transcription factor
Subcellular location
Nucleus
Function
Together with PML, this tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. Functions as a transcriptional coactivator of ETS1 and ETS2 according to . Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA according to . Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through interaction with the MRN complex it may be involved in the regulation of telomeres lengthening. May also regulate TP53-mediated transcription and through CASP8AP2, regulate FAS-mediated apoptosis. Also plays a role in infection by viruses, including human cytomegalovirus and Epstein-Barr virus, through mechanisms that may involve chromatin and/or transcriptional regulation.
Uniprot ID
P23497
Ensemble ID
ENST00000264052.9
HGNC ID
HGNC:11206

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 20 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
STPyne
 Probe Info  		K61(7.35); K70(10.00)  LDD0277  [2]
DBIA
 Probe Info  		C309(1.62)  LDD3310  [3]
BTD
 Probe Info  		C309(0.77)  LDD1700  [4]
AHL-Pu-1
 Probe Info  		C373(3.09)  LDD0170  [5]
HHS-475
 Probe Info  		Y107(0.50); Y752(0.82); Y836(0.93)  LDD0264  [6]
HHS-465
 Probe Info  		Y836(7.46)  LDD2237  [7]
4-Iodoacetamidophenylacetylene
 Probe Info  		C309(0.00); C96(0.00)  LDD0038  [8]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [8]
Lodoacetamide azide
 Probe Info  		C96(0.00); C309(0.00)  LDD0037  [8]
NAIA_4
 Probe Info  		C209(0.00); C238(0.00); C468(0.00)  LDD2226  [9]
NAIA_5
 Probe Info  		C248(0.00); C238(0.00)  LDD2224  [9]
WYneN
 Probe Info  		N.A.  LDD0021  [10]
WYneO
 Probe Info  		N.A.  LDD0022  [10]
Compound 10
 Probe Info  		N.A.  LDD2216  [11]
IPM
 Probe Info  		N.A.  LDD0147  [12]
TFBX
 Probe Info  		N.A.  LDD0148  [12]
Acrolein
 Probe Info  		C780(0.00); C96(0.00); C309(0.00)  LDD0217  [13]
Methacrolein
 Probe Info  		N.A.  LDD0218  [13]
W1
 Probe Info  		N.A.  LDD0236  [14]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C309(0.80)  LDD2117  [4]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C309(1.06)  LDD2152  [4]
 LDCM0025  4SU-RNA DM93 C373(3.09)  LDD0170  [5]
 LDCM0026  4SU-RNA+native RNA DM93 C373(3.10)  LDD0171  [5]
 LDCM0520  AKOS000195272 MDA-MB-231 C309(0.87)  LDD2113  [4]
 LDCM0156  Aniline NCI-H1299 11.78  LDD0403  [1]
 LDCM0108  Chloroacetamide HeLa C96(0.00); C309(0.00)  LDD0222  [13]
 LDCM0632  CL-Sc Hep-G2 C309(20.00); C238(0.52)  LDD2227  [9]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C309(1.98)  LDD1702  [4]
 LDCM0625  F8 Ramos C309(1.35); C96(1.13); C468(1.07); C373(1.36)  LDD2187  [15]
 LDCM0572  Fragment10 Ramos C309(1.78); C96(1.44); C468(1.26); C373(1.94)  LDD2189  [15]
 LDCM0573  Fragment11 Ramos C309(6.20); C96(0.16); C468(2.78)  LDD2190  [15]
 LDCM0574  Fragment12 Ramos C309(0.99); C96(1.62); C468(1.00); C373(1.35)  LDD2191  [15]
 LDCM0575  Fragment13 Ramos C309(1.05); C96(0.95); C468(0.88); C373(1.01)  LDD2192  [15]
 LDCM0576  Fragment14 Ramos C309(0.98); C96(1.68); C468(0.47); C373(1.26)  LDD2193  [15]
 LDCM0579  Fragment20 Ramos C309(1.17); C96(1.52); C468(0.73); C373(0.61)  LDD2194  [15]
 LDCM0580  Fragment21 Ramos C309(1.11); C96(0.81); C468(1.08); C373(1.23)  LDD2195  [15]
 LDCM0582  Fragment23 Ramos C309(1.56); C96(1.15); C468(1.28)  LDD2196  [15]
 LDCM0578  Fragment27 Ramos C309(1.09); C96(0.79); C468(1.03); C373(0.99)  LDD2197  [15]
 LDCM0586  Fragment28 Ramos C309(0.59); C96(0.78); C468(0.99); C373(1.99)  LDD2198  [15]
 LDCM0588  Fragment30 Ramos C309(1.36); C96(1.32); C468(1.07)  LDD2199  [15]
 LDCM0589  Fragment31 Ramos C309(1.13); C96(1.08); C468(1.03); C373(1.51)  LDD2200  [15]
 LDCM0590  Fragment32 Ramos C309(2.27); C96(1.39); C468(1.21)  LDD2201  [15]
 LDCM0468  Fragment33 Ramos C309(1.32); C96(1.18); C468(0.98); C373(0.73)  LDD2202  [15]
 LDCM0596  Fragment38 Ramos C309(1.00); C96(1.02); C468(0.75); C373(0.58)  LDD2203  [15]
 LDCM0566  Fragment4 Ramos C309(1.18); C96(1.15); C468(0.84)  LDD2184  [15]
 LDCM0610  Fragment52 Ramos C309(1.39); C96(1.17); C468(0.99); C373(1.15)  LDD2204  [15]
 LDCM0614  Fragment56 Ramos C309(1.68); C96(1.16); C468(0.96)  LDD2205  [15]
 LDCM0569  Fragment7 Ramos C309(1.20); C96(1.44); C468(1.04); C373(1.40)  LDD2186  [15]
 LDCM0571  Fragment9 Ramos C309(1.29); C96(2.04); C468(1.01)  LDD2188  [15]
 LDCM0116  HHS-0101 DM93 Y107(0.50); Y752(0.82); Y836(0.93)  LDD0264  [6]
 LDCM0117  HHS-0201 DM93 Y107(0.68); Y752(0.74); Y836(0.87)  LDD0265  [6]
 LDCM0118  HHS-0301 DM93 Y107(0.47); Y836(0.87); Y752(0.87)  LDD0266  [6]
 LDCM0119  HHS-0401 DM93 Y107(0.70); Y752(0.81); Y836(0.89)  LDD0267  [6]
 LDCM0120  HHS-0701 DM93 Y107(0.48); Y752(0.79); Y836(0.93)  LDD0268  [6]
 LDCM0022  KB02 Ramos C309(1.51); C96(1.46); C468(1.14)  LDD2182  [15]
 LDCM0023  KB03 MDA-MB-231 C309(2.85); C373(1.51)  LDD1701  [4]
 LDCM0024  KB05 COLO792 C309(1.62)  LDD3310  [3]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C309(0.56)  LDD2121  [4]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C309(0.74)  LDD2097  [4]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C309(0.70)  LDD2100  [4]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C309(0.89)  LDD2101  [4]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C309(0.75)  LDD2107  [4]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C309(0.59)  LDD2109  [4]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C309(0.49)  LDD2111  [4]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C309(0.51)  LDD2115  [4]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C309(0.52)  LDD2123  [4]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C309(0.69)  LDD2125  [4]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C309(1.75)  LDD2126  [4]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C309(0.70)  LDD2127  [4]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C309(0.60)  LDD2129  [4]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C309(0.57)  LDD2133  [4]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C309(0.55)  LDD2135  [4]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C309(0.79)  LDD2136  [4]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C309(0.82)  LDD2137  [4]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C309(0.77)  LDD1700  [4]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C309(0.55)  LDD2140  [4]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C309(0.54)  LDD2141  [4]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C309(2.32)  LDD2144  [4]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C309(1.57)  LDD2145  [4]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C309(0.54)  LDD2146  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
DNA (cytosine-5)-methyltransferase 3A (DNMT3A) C5-methyltransferase family Q9Y6K1
DNA-directed RNA polymerase III subunit RPC3 (POLR3C) Eukaryotic RPC3/POLR3C RNA polymerase subunit family Q9BUI4
Dual specificity tyrosine-phosphorylation-regulated kinase 2 (DYRK2) CMGC Ser/Thr protein kinase family Q92630
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Alpha-actinin-2 (ACTN2) Alpha-actinin family P35609
Alpha-synuclein (SNCA) Synuclein family P37840
Transcription factor
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein C-ets-1 (ETS1) ETS family P14921
Zinc finger C2HC domain-containing protein 1A (ZC2HC1A) ZC2HC1 family Q96GY0
Other
Click To Hide/Show 11 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
PDZ domain-containing protein GIPC2 (GIPC2) GIPC family Q8TF65
Small ubiquitin-related modifier 1 (SUMO1) Ubiquitin family P63165
Small ubiquitin-related modifier 2 (SUMO2) Ubiquitin family P61956
Small ubiquitin-related modifier 3 (SUMO3) Ubiquitin family P55854
CASP8-associated protein 2 (CASP8AP2) . Q9UKL3
Chromobox protein homolog 1 (CBX1) . P83916
Chromobox protein homolog 3 (CBX3) . Q13185
Chromobox protein homolog 5 (CBX5) . P45973
Lethal(3)malignant brain tumor-like protein 3 (L3MBTL3) . Q96JM7
Receptor-transporting protein 5 (RTP5) . Q14D33
TRAF3-interacting JNK-activating modulator (TRAF3IP3) . Q9Y228

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
6 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
7 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
8 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
9 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
10 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
11 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
12 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
13 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
14 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
15 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578