Details of the Target

General Information of Target

Target ID LDTP02387
Target Name Peroxisomal coenzyme A diphosphatase NUDT7 (NUDT7)
Gene Name NUDT7
Gene ID 283927
Synonyms
Peroxisomal coenzyme A diphosphatase NUDT7; EC 3.6.1.-; Nucleoside diphosphate-linked moiety X motif 7; Nudix motif 7
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSRLGLPEEPVRNSLLDDAKARLRKYDIGGKYSHLPYNKYSVLLPLVAKEGKLHLLFTVR
SEKLRRAPGEVCFPGGKRDPTDMDDAATALREAQEEVGLRPHQVEVVCCLVPCLIDTDTL
ITPFVGLIDHNFQAQPNPAEVKDVFLVPLAYFLHPQVHDQHYVTRLGHRFINHIFEYTNP
EDGVTYQIKGMTANLAVLVAFIILEKKPTFEVQFNLNDVLASSEELFLKVHKKATSRL
Target Bioclass
Enzyme
Family
Nudix hydrolase family, PCD1 subfamily
Subcellular location
Peroxisome
Function
Fatty acyl-coenzyme A (CoA) diphosphatase that hydrolyzes fatty acyl-CoA to yield acyl-4'-phosphopantetheine and adenosine 3',5'-bisphosphate. Cleaves CoA, CoA esters and oxidized CoA with similar efficiencies. Preferentially hydrolyzes medium-chain acyl-CoAs and bile acid-CoAs. Has no activity toward NDP-sugars, CDP-alcohols, (deoxy)nucleoside 5'-triphosphates, nucleoside 5'-di or monophosphates, diadenosine polyphosphates, NAD, NADH, NADP, NADPH or thymidine-5'-monophospho-p-nitrophenyl ester. May be required to eliminate oxidized CoA from peroxisomes, or regulate CoA and acyl-CoA levels in this organelle in response to metabolic demand. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA. Exhibits decapping activity towards dpCoA-capped RNAs in vitro.
Uniprot ID
P0C024
Ensemble ID
ENST00000268533.9
HGNC ID
HGNC:8054

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A3KAW SNV: p.E96D .
HT115 SNV: p.K233Q .
KATOIII SNV: p.R60P .
TOV21G SNV: p.G5C .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C72(7.58)  LDD3335  [1]
IA-alkyne
 Probe Info  		C72(11.50)  LDD1704  [2]
IPM
 Probe Info  		N.A.  LDD2156  [3]
TFBX
 Probe Info  		N.A.  LDD0148  [4]
VSF
 Probe Info  		N.A.  LDD0007  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C72(0.99)  LDD1507  [6]
 LDCM0237  AC12 HEK-293T C72(1.10)  LDD1510  [6]
 LDCM0270  AC15 HEK-293T C72(1.05)  LDD1513  [6]
 LDCM0276  AC17 HEK-293T C72(1.15)  LDD1515  [6]
 LDCM0280  AC20 HEK-293T C72(1.07)  LDD1519  [6]
 LDCM0283  AC23 HEK-293T C72(1.02)  LDD1522  [6]
 LDCM0284  AC24 HEK-293T C72(0.92)  LDD1523  [6]
 LDCM0285  AC25 HEK-293T C72(0.95)  LDD1524  [6]
 LDCM0288  AC28 HEK-293T C72(0.93)  LDD1527  [6]
 LDCM0292  AC31 HEK-293T C72(1.07)  LDD1531  [6]
 LDCM0293  AC32 HEK-293T C72(0.91)  LDD1532  [6]
 LDCM0294  AC33 HEK-293T C72(0.99)  LDD1533  [6]
 LDCM0297  AC36 HEK-293T C72(0.93)  LDD1536  [6]
 LDCM0300  AC39 HEK-293T C72(1.02)  LDD1539  [6]
 LDCM0301  AC4 HEK-293T C72(0.89)  LDD1540  [6]
 LDCM0302  AC40 HEK-293T C72(0.97)  LDD1541  [6]
 LDCM0303  AC41 HEK-293T C72(1.07)  LDD1542  [6]
 LDCM0306  AC44 HEK-293T C72(0.95)  LDD1545  [6]
 LDCM0309  AC47 HEK-293T C72(1.14)  LDD1548  [6]
 LDCM0310  AC48 HEK-293T C72(1.02)  LDD1549  [6]
 LDCM0311  AC49 HEK-293T C72(1.00)  LDD1550  [6]
 LDCM0315  AC52 HEK-293T C72(1.03)  LDD1554  [6]
 LDCM0318  AC55 HEK-293T C72(1.09)  LDD1557  [6]
 LDCM0319  AC56 HEK-293T C72(1.01)  LDD1558  [6]
 LDCM0320  AC57 HEK-293T C72(1.04)  LDD1559  [6]
 LDCM0324  AC60 HEK-293T C72(0.98)  LDD1563  [6]
 LDCM0327  AC63 HEK-293T C72(1.03)  LDD1566  [6]
 LDCM0328  AC64 HEK-293T C72(0.96)  LDD1567  [6]
 LDCM0334  AC7 HEK-293T C72(1.00)  LDD1568  [6]
 LDCM0345  AC8 HEK-293T C72(0.93)  LDD1569  [6]
 LDCM0356  AKOS034007680 HEK-293T C72(1.02)  LDD1570  [6]
 LDCM0275  AKOS034007705 HEK-293T C72(1.03)  LDD1514  [6]
 LDCM0369  CL100 HEK-293T C72(1.11)  LDD1573  [6]
 LDCM0371  CL102 HEK-293T C72(1.30)  LDD1575  [6]
 LDCM0372  CL103 HEK-293T C72(0.94)  LDD1576  [6]
 LDCM0373  CL104 HEK-293T C72(1.17)  LDD1577  [6]
 LDCM0375  CL106 HEK-293T C72(1.11)  LDD1579  [6]
 LDCM0376  CL107 HEK-293T C72(0.94)  LDD1580  [6]
 LDCM0377  CL108 HEK-293T C72(1.00)  LDD1581  [6]
 LDCM0379  CL11 HEK-293T C72(1.29)  LDD1583  [6]
 LDCM0380  CL110 HEK-293T C72(1.56)  LDD1584  [6]
 LDCM0381  CL111 HEK-293T C72(0.96)  LDD1585  [6]
 LDCM0382  CL112 HEK-293T C72(1.13)  LDD1586  [6]
 LDCM0384  CL114 HEK-293T C72(2.09)  LDD1588  [6]
 LDCM0385  CL115 HEK-293T C72(0.92)  LDD1589  [6]
 LDCM0386  CL116 HEK-293T C72(0.80)  LDD1590  [6]
 LDCM0388  CL118 HEK-293T C72(1.13)  LDD1592  [6]
 LDCM0389  CL119 HEK-293T C72(0.89)  LDD1593  [6]
 LDCM0390  CL12 HEK-293T C72(0.99)  LDD1594  [6]
 LDCM0391  CL120 HEK-293T C72(1.09)  LDD1595  [6]
 LDCM0393  CL122 HEK-293T C72(1.11)  LDD1597  [6]
 LDCM0394  CL123 HEK-293T C72(0.92)  LDD1598  [6]
 LDCM0395  CL124 HEK-293T C72(1.53)  LDD1599  [6]
 LDCM0397  CL126 HEK-293T C72(1.00)  LDD1601  [6]
 LDCM0398  CL127 HEK-293T C72(0.91)  LDD1602  [6]
 LDCM0399  CL128 HEK-293T C72(1.13)  LDD1603  [6]
 LDCM0401  CL14 HEK-293T C72(1.05)  LDD1605  [6]
 LDCM0402  CL15 HEK-293T C72(0.95)  LDD1606  [6]
 LDCM0403  CL16 HEK-293T C72(1.10)  LDD1607  [6]
 LDCM0404  CL17 HEK-293T C72(1.06)  LDD1608  [6]
 LDCM0407  CL2 HEK-293T C72(1.24)  LDD1611  [6]
 LDCM0408  CL20 HEK-293T C72(1.11)  LDD1612  [6]
 LDCM0411  CL23 HEK-293T C72(1.17)  LDD1615  [6]
 LDCM0412  CL24 HEK-293T C72(1.00)  LDD1616  [6]
 LDCM0414  CL26 HEK-293T C72(1.06)  LDD1618  [6]
 LDCM0415  CL27 HEK-293T C72(0.98)  LDD1619  [6]
 LDCM0416  CL28 HEK-293T C72(1.05)  LDD1620  [6]
 LDCM0417  CL29 HEK-293T C72(1.05)  LDD1621  [6]
 LDCM0418  CL3 HEK-293T C72(1.28)  LDD1622  [6]
 LDCM0421  CL32 HEK-293T C72(0.99)  LDD1625  [6]
 LDCM0424  CL35 HEK-293T C72(1.21)  LDD1628  [6]
 LDCM0425  CL36 HEK-293T C72(0.92)  LDD1629  [6]
 LDCM0428  CL39 HEK-293T C72(0.97)  LDD1632  [6]
 LDCM0429  CL4 HEK-293T C72(1.12)  LDD1633  [6]
 LDCM0430  CL40 HEK-293T C72(1.03)  LDD1634  [6]
 LDCM0431  CL41 HEK-293T C72(1.01)  LDD1635  [6]
 LDCM0434  CL44 HEK-293T C72(0.99)  LDD1638  [6]
 LDCM0437  CL47 HEK-293T C72(1.16)  LDD1641  [6]
 LDCM0438  CL48 HEK-293T C72(1.46)  LDD1642  [6]
 LDCM0440  CL5 HEK-293T C72(1.17)  LDD1644  [6]
 LDCM0441  CL50 HEK-293T C72(1.12)  LDD1645  [6]
 LDCM0443  CL52 HEK-293T C72(1.10)  LDD1646  [6]
 LDCM0444  CL53 HEK-293T C72(1.09)  LDD1647  [6]
 LDCM0447  CL56 HEK-293T C72(1.02)  LDD1650  [6]
 LDCM0450  CL59 HEK-293T C72(1.24)  LDD1653  [6]
 LDCM0452  CL60 HEK-293T C72(1.00)  LDD1655  [6]
 LDCM0454  CL62 HEK-293T C72(1.03)  LDD1657  [6]
 LDCM0455  CL63 HEK-293T C72(0.90)  LDD1658  [6]
 LDCM0456  CL64 HEK-293T C72(1.26)  LDD1659  [6]
 LDCM0457  CL65 HEK-293T C72(1.04)  LDD1660  [6]
 LDCM0460  CL68 HEK-293T C72(0.92)  LDD1663  [6]
 LDCM0464  CL71 HEK-293T C72(1.57)  LDD1667  [6]
 LDCM0465  CL72 HEK-293T C72(0.95)  LDD1668  [6]
 LDCM0467  CL74 HEK-293T C72(0.99)  LDD1670  [6]
 LDCM0469  CL76 HEK-293T C72(1.11)  LDD1672  [6]
 LDCM0470  CL77 HEK-293T C72(0.95)  LDD1673  [6]
 LDCM0473  CL8 HEK-293T C72(2.82)  LDD1676  [6]
 LDCM0474  CL80 HEK-293T C72(1.04)  LDD1677  [6]
 LDCM0477  CL83 HEK-293T C72(1.32)  LDD1680  [6]
 LDCM0478  CL84 HEK-293T C72(1.21)  LDD1681  [6]
 LDCM0480  CL86 HEK-293T C72(1.14)  LDD1683  [6]
 LDCM0481  CL87 HEK-293T C72(0.94)  LDD1684  [6]
 LDCM0482  CL88 HEK-293T C72(1.10)  LDD1685  [6]
 LDCM0483  CL89 HEK-293T C72(1.00)  LDD1686  [6]
 LDCM0487  CL92 HEK-293T C72(0.97)  LDD1690  [6]
 LDCM0490  CL95 HEK-293T C72(1.96)  LDD1693  [6]
 LDCM0491  CL96 HEK-293T C72(1.41)  LDD1694  [6]
 LDCM0493  CL98 HEK-293T C72(1.11)  LDD1696  [6]
 LDCM0494  CL99 HEK-293T C72(1.15)  LDD1697  [6]
 LDCM0495  E2913 HEK-293T C72(0.97)  LDD1698  [6]
 LDCM0468  Fragment33 HEK-293T C72(0.86)  LDD1671  [6]
 LDCM0427  Fragment51 HEK-293T C72(0.93)  LDD1631  [6]
 LDCM0022  KB02 HEK-293T C72(0.86)  LDD1492  [6]
 LDCM0023  KB03 HEK-293T C72(0.91)  LDD1497  [6]
 LDCM0024  KB05 MONO-MAC-6 C72(7.58)  LDD3335  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
3 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
4 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
5 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402