Details of the Target

General Information of Target

Target ID LDTP02366
Target Name Pro-cathepsin H (CTSH)
Gene Name CTSH
Gene ID 1512
Synonyms
CPSB; Pro-cathepsin H [Cleaved into: Cathepsin H mini chain; Cathepsin H; EC 3.4.22.16; Cathepsin H heavy chain; Cathepsin H light chain]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MWATLPLLCAGAWLLGVPVCGAAELCVNSLEKFHFKSWMSKHRKTYSTEEYHHRLQTFAS
NWRKINAHNNGNHTFKMALNQFSDMSFAEIKHKYLWSEPQNCSATKSNYLRGTGPYPPSV
DWRKKGNFVSPVKNQGACGSCWTFSTTGALESAIAIATGKMLSLAEQQLVDCAQDFNNHG
CQGGLPSQAFEYILYNKGIMGEDTYPYQGKDGYCKFQPGKAIGFVKDVANITIYDEEAMV
EAVALYNPVSFAFEVTQDFMMYRTGIYSSTSCHKTPDKVNHAVLAVGYGEKNGIPYWIVK
NSWGPQWGMNGYFLIERGKNMCGLAACASYPIPLV
Target Type
Literature-reported
Target Bioclass
Enzyme
Family
Peptidase C1 family
Subcellular location
Lysosome
Function Important for the overall degradation of proteins in lysosomes.
TTD ID
T40103
Uniprot ID
P09668
DrugMap ID
TT3G406
Ensemble ID
ENST00000220166.10
HGNC ID
HGNC:2535
ChEMBL ID
CHEMBL2225

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
P11
 Probe Info  		20.00  LDD0201  [1]
DBIA
 Probe Info  		C272(1.48)  LDD3330  [2]
FBPP2
 Probe Info  		5.70  LDD0054  [3]
AHL-Pu-1
 Probe Info  		C172(3.87)  LDD0171  [4]
ATP probe
 Probe Info  		N.A.  LDD0199  [5]
IA-alkyne
 Probe Info  		C272(0.00); C172(0.00)  LDD0162  [6]
IPM
 Probe Info  		N.A.  LDD2156  [7]
NAIA_5
 Probe Info  		N.A.  LDD2223  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0026  4SU-RNA+native RNA DM93 C172(3.87)  LDD0171  [4]
 LDCM0281  AC21 HEK-293T C141(0.77)  LDD1520  [9]
 LDCM0289  AC29 HEK-293T C141(1.02)  LDD1528  [9]
 LDCM0298  AC37 HEK-293T C141(1.40)  LDD1537  [9]
 LDCM0307  AC45 HEK-293T C141(1.06)  LDD1546  [9]
 LDCM0312  AC5 HEK-293T C141(1.00)  LDD1551  [9]
 LDCM0316  AC53 HEK-293T C141(0.89)  LDD1555  [9]
 LDCM0325  AC61 HEK-293T C141(0.78)  LDD1564  [9]
 LDCM0248  AKOS034007472 HEK-293T C141(1.00)  LDD1511  [9]
 LDCM0088  C45 HEK-293T 20.00  LDD0201  [1]
 LDCM0367  CL1 HEK-293T C102(1.06)  LDD1571  [9]
 LDCM0370  CL101 HEK-293T C102(0.90)  LDD1574  [9]
 LDCM0374  CL105 HEK-293T C102(0.78)  LDD1578  [9]
 LDCM0378  CL109 HEK-293T C102(0.68)  LDD1582  [9]
 LDCM0383  CL113 HEK-293T C102(0.89)  LDD1587  [9]
 LDCM0387  CL117 HEK-293T C102(0.83)  LDD1591  [9]
 LDCM0392  CL121 HEK-293T C102(0.96)  LDD1596  [9]
 LDCM0396  CL125 HEK-293T C102(0.99)  LDD1600  [9]
 LDCM0400  CL13 HEK-293T C102(0.95)  LDD1604  [9]
 LDCM0409  CL21 HEK-293T C141(0.71)  LDD1613  [9]
 LDCM0413  CL25 HEK-293T C102(0.94)  LDD1617  [9]
 LDCM0422  CL33 HEK-293T C141(1.18)  LDD1626  [9]
 LDCM0426  CL37 HEK-293T C102(1.03)  LDD1630  [9]
 LDCM0435  CL45 HEK-293T C141(0.78)  LDD1639  [9]
 LDCM0439  CL49 HEK-293T C102(0.82)  LDD1643  [9]
 LDCM0448  CL57 HEK-293T C141(1.15)  LDD1651  [9]
 LDCM0453  CL61 HEK-293T C102(0.83)  LDD1656  [9]
 LDCM0461  CL69 HEK-293T C141(1.04)  LDD1664  [9]
 LDCM0466  CL73 HEK-293T C102(0.93)  LDD1669  [9]
 LDCM0475  CL81 HEK-293T C141(1.05)  LDD1678  [9]
 LDCM0479  CL85 HEK-293T C102(1.13)  LDD1682  [9]
 LDCM0484  CL9 HEK-293T C141(0.80)  LDD1687  [9]
 LDCM0488  CL93 HEK-293T C141(1.34)  LDD1691  [9]
 LDCM0492  CL97 HEK-293T C102(0.94)  LDD1695  [9]
 LDCM0022  KB02 KP-4 C272(1.71)  LDD2428  [2]
 LDCM0023  KB03 MIA PaCa-2 C272(2.06)  LDD2913  [2]
 LDCM0024  KB05 MIA PaCa-2 C272(1.48)  LDD3330  [2]
 LDCM0008  Tranylcypromine SH-SY5Y 5.70  LDD0054  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Kallikrein-6 (KLK6) Peptidase S1 family Q92876
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cytoplasmic dynein 1 heavy chain 1 (DYNC1H1) Dynein heavy chain family Q14204

The Drug(s) Related To This Target

Investigative
Click To Hide/Show 5 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Pmid16290936c1b Small molecular drug D0G2QG
Carbobenzyloxy-(L)-leucinyl-(L)Leucinyl Methoxymethylketone . DB08526
N-[1-hydroxycarboxyethyl-carbonyl]Leucylamino-2-methyl-butane . DB04126
N1-(1-dimethylcarbamoyl-2-phenyl-ethyl)-2-oxo-n4-(2-pyridin-2-yl-ethyl)-succinamide . DB02140
P-toluenesulfonic Acid . DB03120

References

1 Discovery of Potent and Selective Inhibitors against Protein-Derived Electrophilic Cofactors. J Am Chem Soc. 2022 Mar 30;144(12):5377-5388. doi: 10.1021/jacs.1c12748. Epub 2022 Mar 2.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
4 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
5 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
6 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
7 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
8 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
9 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402