Details of the Target

General Information of Target

Target ID LDTP02121
Target Name Transcription factor AP-2-alpha (TFAP2A)
Gene Name TFAP2A
Gene ID 7020
Synonyms
AP2TF; TFAP2; Transcription factor AP-2-alpha; AP2-alpha; AP-2 transcription factor; Activating enhancer-binding protein 2-alpha; Activator protein 2; AP-2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLWKLTDNIKYEDCEDRHDGTSNGTARLPQLGTVGQSPYTSAPPLSHTPNADFQPPYFPP
PYQPIYPQSQDPYSHVNDPYSLNPLHAQPQPQHPGWPGQRQSQESGLLHTHRGLPHQLSG
LDPRRDYRRHEDLLHGPHALSSGLGDLSIHSLPHAIEEVPHVEDPGINIPDQTVIKKGPV
SLSKSNSNAVSAIPINKDNLFGGVVNPNEVFCSVPGRLSLLSSTSKYKVTVAEVQRRLSP
PECLNASLLGGVLRRAKSKNGGRSLREKLDKIGLNLPAGRRKAANVTLLTSLVEGEAVHL
ARDFGYVCETEFPAKAVAEFLNRQHSDPNEQVTRKNMLLATKQICKEFTDLLAQDRSPLG
NSRPNPILEPGIQSCLTHFNLISHGFGSPAVCAAVTALQNYLTEALKAMDKMYLSNNPNS
HTDNNAKSSDKEEKHRK
Target Type
Literature-reported
Target Bioclass
Transcription factor
Family
AP-2 family
Subcellular location
Nucleus
Function
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.
TTD ID
T71317
Uniprot ID
P05549
DrugMap ID
TTDY4BS
Ensemble ID
ENST00000319516.8
HGNC ID
HGNC:11742

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
AHL-Pu-1
 Probe Info  		C14(3.36)  LDD0170  [1]
Acrolein
 Probe Info  		N.A.  LDD0222  [2]
DBIA
 Probe Info  		C14(1.31)  LDD0080  [3]
5E-2FA
 Probe Info  		N.A.  LDD2235  [4]
m-APA
 Probe Info  		N.A.  LDD2231  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [5]
IPM
 Probe Info  		C308(0.00); C212(0.00); C243(0.00)  LDD0147  [6]
TFBX
 Probe Info  		C243(0.00); C308(0.00); C212(0.00)  LDD0148  [6]
AOyne
 Probe Info  		15.00  LDD0443  [7]
NAIA_5
 Probe Info  		N.A.  LDD2223  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA DM93 C14(3.36)  LDD0170  [1]
 LDCM0026  4SU-RNA+native RNA DM93 C14(2.07)  LDD0171  [1]
 LDCM0214  AC1 HEK-293T C14(0.87)  LDD1507  [9]
 LDCM0215  AC10 HEK-293T C14(0.93)  LDD1508  [9]
 LDCM0226  AC11 HEK-293T C14(1.01); C308(1.10)  LDD1509  [9]
 LDCM0259  AC14 HEK-293T C14(0.97)  LDD1512  [9]
 LDCM0270  AC15 HEK-293T C14(0.76)  LDD1513  [9]
 LDCM0276  AC17 HEK-293T C14(0.95)  LDD1515  [9]
 LDCM0277  AC18 HEK-293T C14(1.11)  LDD1516  [9]
 LDCM0278  AC19 HEK-293T C14(0.88); C308(1.18)  LDD1517  [9]
 LDCM0279  AC2 HEK-293T C14(0.61)  LDD1518  [9]
 LDCM0281  AC21 HEK-293T C14(0.96)  LDD1520  [9]
 LDCM0282  AC22 HEK-293T C14(1.23)  LDD1521  [9]
 LDCM0283  AC23 HEK-293T C14(0.77)  LDD1522  [9]
 LDCM0284  AC24 HEK-293T C14(1.09)  LDD1523  [9]
 LDCM0285  AC25 HEK-293T C14(1.04)  LDD1524  [9]
 LDCM0286  AC26 HEK-293T C14(1.06)  LDD1525  [9]
 LDCM0287  AC27 HEK-293T C14(0.90); C308(1.10)  LDD1526  [9]
 LDCM0289  AC29 HEK-293T C14(0.86)  LDD1528  [9]
 LDCM0290  AC3 HEK-293T C14(0.86); C308(1.09)  LDD1529  [9]
 LDCM0291  AC30 HEK-293T C14(0.88)  LDD1530  [9]
 LDCM0292  AC31 HEK-293T C14(1.05)  LDD1531  [9]
 LDCM0293  AC32 HEK-293T C14(0.83)  LDD1532  [9]
 LDCM0294  AC33 HEK-293T C14(1.02)  LDD1533  [9]
 LDCM0295  AC34 HEK-293T C14(1.15)  LDD1534  [9]
 LDCM0296  AC35 HEK-293T C14(1.21); C308(1.10)  LDD1535  [9]
 LDCM0298  AC37 HEK-293T C14(0.79)  LDD1537  [9]
 LDCM0299  AC38 HEK-293T C14(0.99)  LDD1538  [9]
 LDCM0300  AC39 HEK-293T C14(0.85)  LDD1539  [9]
 LDCM0302  AC40 HEK-293T C14(0.90)  LDD1541  [9]
 LDCM0303  AC41 HEK-293T C14(1.01)  LDD1542  [9]
 LDCM0304  AC42 HEK-293T C14(1.05)  LDD1543  [9]
 LDCM0305  AC43 HEK-293T C14(1.05); C308(1.14)  LDD1544  [9]
 LDCM0307  AC45 HEK-293T C14(0.82)  LDD1546  [9]
 LDCM0308  AC46 HEK-293T C14(1.00)  LDD1547  [9]
 LDCM0309  AC47 HEK-293T C14(0.78)  LDD1548  [9]
 LDCM0310  AC48 HEK-293T C14(1.07)  LDD1549  [9]
 LDCM0311  AC49 HEK-293T C14(1.01)  LDD1550  [9]
 LDCM0312  AC5 HEK-293T C14(0.60)  LDD1551  [9]
 LDCM0313  AC50 HEK-293T C14(1.09)  LDD1552  [9]
 LDCM0314  AC51 HEK-293T C14(1.12); C308(1.11)  LDD1553  [9]
 LDCM0316  AC53 HEK-293T C14(1.08)  LDD1555  [9]
 LDCM0317  AC54 HEK-293T C14(0.93)  LDD1556  [9]
 LDCM0318  AC55 HEK-293T C14(0.82)  LDD1557  [9]
 LDCM0319  AC56 HEK-293T C14(1.11)  LDD1558  [9]
 LDCM0320  AC57 HEK-293T C14(0.99)  LDD1559  [9]
 LDCM0321  AC58 HEK-293T C14(1.00)  LDD1560  [9]
 LDCM0322  AC59 HEK-293T C14(1.06); C308(1.10)  LDD1561  [9]
 LDCM0323  AC6 HEK-293T C14(0.77)  LDD1562  [9]
 LDCM0325  AC61 HEK-293T C14(1.10)  LDD1564  [9]
 LDCM0326  AC62 HEK-293T C14(0.96)  LDD1565  [9]
 LDCM0327  AC63 HEK-293T C14(0.97)  LDD1566  [9]
 LDCM0328  AC64 HEK-293T C14(1.12)  LDD1567  [9]
 LDCM0334  AC7 HEK-293T C14(0.47)  LDD1568  [9]
 LDCM0345  AC8 HEK-293T C14(1.01)  LDD1569  [9]
 LDCM0248  AKOS034007472 HEK-293T C14(0.87)  LDD1511  [9]
 LDCM0356  AKOS034007680 HEK-293T C14(1.09)  LDD1570  [9]
 LDCM0275  AKOS034007705 HEK-293T C14(0.98)  LDD1514  [9]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [2]
 LDCM0368  CL10 HEK-293T C14(0.79)  LDD1572  [9]
 LDCM0371  CL102 HEK-293T C212(0.88); C308(0.89)  LDD1575  [9]
 LDCM0372  CL103 HEK-293T C308(0.99)  LDD1576  [9]
 LDCM0375  CL106 HEK-293T C212(0.91); C308(1.01)  LDD1579  [9]
 LDCM0376  CL107 HEK-293T C308(1.19)  LDD1580  [9]
 LDCM0379  CL11 HEK-293T C14(0.67)  LDD1583  [9]
 LDCM0380  CL110 HEK-293T C212(0.93); C308(1.08)  LDD1584  [9]
 LDCM0381  CL111 HEK-293T C308(1.16)  LDD1585  [9]
 LDCM0384  CL114 HEK-293T C212(1.04); C308(1.16)  LDD1588  [9]
 LDCM0385  CL115 HEK-293T C308(1.07)  LDD1589  [9]
 LDCM0388  CL118 HEK-293T C212(0.92); C308(0.97)  LDD1592  [9]
 LDCM0389  CL119 HEK-293T C308(0.91)  LDD1593  [9]
 LDCM0390  CL12 HEK-293T C14(0.87)  LDD1594  [9]
 LDCM0393  CL122 HEK-293T C212(0.96); C308(1.00)  LDD1597  [9]
 LDCM0394  CL123 HEK-293T C308(0.92)  LDD1598  [9]
 LDCM0397  CL126 HEK-293T C212(0.93); C308(0.83)  LDD1601  [9]
 LDCM0398  CL127 HEK-293T C308(0.95)  LDD1602  [9]
 LDCM0401  CL14 HEK-293T C212(0.90); C308(1.23)  LDD1605  [9]
 LDCM0402  CL15 HEK-293T C308(1.12)  LDD1606  [9]
 LDCM0404  CL17 HEK-293T C14(0.87)  LDD1608  [9]
 LDCM0405  CL18 HEK-293T C14(1.05)  LDD1609  [9]
 LDCM0406  CL19 HEK-293T C14(0.90); C308(1.25)  LDD1610  [9]
 LDCM0407  CL2 HEK-293T C212(0.87); C308(0.91)  LDD1611  [9]
 LDCM0409  CL21 HEK-293T C14(0.79)  LDD1613  [9]
 LDCM0410  CL22 HEK-293T C14(0.67)  LDD1614  [9]
 LDCM0411  CL23 HEK-293T C14(0.52)  LDD1615  [9]
 LDCM0412  CL24 HEK-293T C14(0.78)  LDD1616  [9]
 LDCM0414  CL26 HEK-293T C212(1.05); C308(0.68)  LDD1618  [9]
 LDCM0415  CL27 HEK-293T C308(0.97)  LDD1619  [9]
 LDCM0417  CL29 HEK-293T C14(0.95)  LDD1621  [9]
 LDCM0418  CL3 HEK-293T C308(0.95)  LDD1622  [9]
 LDCM0419  CL30 HEK-293T C14(0.87)  LDD1623  [9]
 LDCM0420  CL31 HEK-293T C14(0.87); C308(1.14)  LDD1624  [9]
 LDCM0422  CL33 HEK-293T C14(0.69)  LDD1626  [9]
 LDCM0423  CL34 HEK-293T C14(0.79)  LDD1627  [9]
 LDCM0424  CL35 HEK-293T C14(0.63)  LDD1628  [9]
 LDCM0425  CL36 HEK-293T C14(0.76)  LDD1629  [9]
 LDCM0428  CL39 HEK-293T C308(0.59)  LDD1632  [9]
 LDCM0431  CL41 HEK-293T C14(0.94)  LDD1635  [9]
 LDCM0432  CL42 HEK-293T C14(1.02)  LDD1636  [9]
 LDCM0433  CL43 HEK-293T C14(0.87); C308(1.17)  LDD1637  [9]
 LDCM0435  CL45 HEK-293T C14(0.86)  LDD1639  [9]
 LDCM0436  CL46 HEK-293T C14(0.84)  LDD1640  [9]
 LDCM0437  CL47 HEK-293T C14(0.81)  LDD1641  [9]
 LDCM0438  CL48 HEK-293T C14(0.79)  LDD1642  [9]
 LDCM0440  CL5 HEK-293T C14(0.95)  LDD1644  [9]
 LDCM0441  CL50 HEK-293T C212(0.86); C308(0.85)  LDD1645  [9]
 LDCM0444  CL53 HEK-293T C14(0.83)  LDD1647  [9]
 LDCM0445  CL54 HEK-293T C14(1.02)  LDD1648  [9]
 LDCM0446  CL55 HEK-293T C14(0.85); C308(1.22)  LDD1649  [9]
 LDCM0448  CL57 HEK-293T C14(0.74)  LDD1651  [9]
 LDCM0449  CL58 HEK-293T C14(0.78)  LDD1652  [9]
 LDCM0450  CL59 HEK-293T C14(0.82)  LDD1653  [9]
 LDCM0451  CL6 HEK-293T C14(0.74)  LDD1654  [9]
 LDCM0452  CL60 HEK-293T C14(0.72)  LDD1655  [9]
 LDCM0454  CL62 HEK-293T C212(0.87); C308(0.85)  LDD1657  [9]
 LDCM0455  CL63 HEK-293T C308(0.82)  LDD1658  [9]
 LDCM0457  CL65 HEK-293T C14(1.02)  LDD1660  [9]
 LDCM0458  CL66 HEK-293T C14(1.35)  LDD1661  [9]
 LDCM0459  CL67 HEK-293T C14(0.86); C308(1.12)  LDD1662  [9]
 LDCM0461  CL69 HEK-293T C14(0.81)  LDD1664  [9]
 LDCM0462  CL7 HEK-293T C14(0.95); C308(1.18)  LDD1665  [9]
 LDCM0463  CL70 HEK-293T C14(0.81)  LDD1666  [9]
 LDCM0464  CL71 HEK-293T C14(0.71)  LDD1667  [9]
 LDCM0465  CL72 HEK-293T C14(0.85)  LDD1668  [9]
 LDCM0467  CL74 HEK-293T C212(0.88); C308(0.85)  LDD1670  [9]
 LDCM0470  CL77 HEK-293T C14(0.91)  LDD1673  [9]
 LDCM0471  CL78 HEK-293T C14(0.88)  LDD1674  [9]
 LDCM0472  CL79 HEK-293T C14(0.80); C308(1.23)  LDD1675  [9]
 LDCM0475  CL81 HEK-293T C14(0.72)  LDD1678  [9]
 LDCM0476  CL82 HEK-293T C14(0.70)  LDD1679  [9]
 LDCM0477  CL83 HEK-293T C14(0.70)  LDD1680  [9]
 LDCM0478  CL84 HEK-293T C14(0.73)  LDD1681  [9]
 LDCM0480  CL86 HEK-293T C212(0.94); C308(1.17)  LDD1683  [9]
 LDCM0481  CL87 HEK-293T C308(0.92)  LDD1684  [9]
 LDCM0483  CL89 HEK-293T C14(0.94)  LDD1686  [9]
 LDCM0484  CL9 HEK-293T C14(0.70)  LDD1687  [9]
 LDCM0485  CL90 HEK-293T C14(0.68)  LDD1688  [9]
 LDCM0486  CL91 HEK-293T C14(0.93); C308(1.23)  LDD1689  [9]
 LDCM0488  CL93 HEK-293T C14(0.69)  LDD1691  [9]
 LDCM0489  CL94 HEK-293T C14(0.65)  LDD1692  [9]
 LDCM0490  CL95 HEK-293T C14(0.56)  LDD1693  [9]
 LDCM0491  CL96 HEK-293T C14(0.71)  LDD1694  [9]
 LDCM0493  CL98 HEK-293T C212(0.87); C308(1.08)  LDD1696  [9]
 LDCM0494  CL99 HEK-293T C308(0.80)  LDD1697  [9]
 LDCM0495  E2913 HEK-293T C308(0.95)  LDD1698  [9]
 LDCM0468  Fragment33 HEK-293T C308(0.99)  LDD1671  [9]
 LDCM0427  Fragment51 HEK-293T C212(0.89); C308(0.89)  LDD1631  [9]
 LDCM0022  KB02 HCT 116 C14(1.31)  LDD0080  [3]
 LDCM0023  KB03 HCT 116 C14(1.36)  LDD0081  [3]
 LDCM0024  KB05 HCT 116 C14(1.43)  LDD0082  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Glutathione S-transferase omega-2 (GSTO2) Omega family Q9H4Y5
SUMO-conjugating enzyme UBC9 (UBE2I) Ubiquitin-conjugating enzyme family P63279
Histone acetyltransferase p300 (EP300) . Q09472
Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) . Q13064
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein max (MAX) MAX family P61244
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein INCA1 (INCA1) INCA family Q0VD86
Nucleophosmin (NPM1) Nucleoplasmin family P06748

References

1 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
2 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
3 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
4 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
5 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
6 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
7 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
8 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
9 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402