Details of the Target

General Information of Target

Target ID LDTP01355
Target Name Dysferlin (DYSF)
Gene Name DYSF
Gene ID 8291
Synonyms
FER1L1; Dysferlin; Dystrophy-associated fer-1-like protein; Fer-1-like protein 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLD
QGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQ
VSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGP
GAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPV
VKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGE
FRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSED
KEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFV
EVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRIIDWDRLTHNDIVATTYL
SMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYT
ELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYS
ATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVV
LSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGC
SQPLGDIHETPSATHLDQYLYQLRTHHLSQITEAALALKLGHSELPAALEQAEDWLLRLR
ALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPM
EKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENETKLALVGNWGTTGLT
YPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRL
PGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPP
ERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFH
LEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRP
TISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWD
QTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPL
TRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYM
VPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNPNF
DICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSP
QGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFD
TLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAI
PMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIP
CTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSKFGARCGL
PQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPN
PHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPP
FNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRS
LGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDD
FLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKI
LAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILW
RRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS
Target Type
Clinical trial
Target Bioclass
Transporter and channel
Family
Ferlin family
Subcellular location
Cell membrane, sarcolemma
Function
Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress.
TTD ID
T34601
Uniprot ID
O75923
DrugMap ID
TTA7MXQ
Ensemble ID
ENST00000258104.8
HGNC ID
HGNC:3097

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K1057(1.02); K1673(7.76); K85(5.88)  LDD0277  [1]
DBIA
 Probe Info  		C1430(2.29); C660(2.08); C1923(2.61); C2010(3.05)  LDD3312  [2]
BTD
 Probe Info  		C1420(1.26)  LDD1700  [3]
IPM
 Probe Info  		C1578(4.29); C456(1.49)  LDD1701  [3]
CY4
 Probe Info  		N.A.  LDD0247  [4]
NAIA_4
 Probe Info  		N.A.  LDD2226  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C1491(1.40); C1420(0.70)  LDD2142  [3]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C1420(0.91)  LDD2112  [3]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C1491(1.09); C1420(0.99)  LDD2117  [3]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C1491(1.22); C1420(1.22)  LDD2152  [3]
 LDCM0510  3-(4-(Hydroxydiphenylmethyl)piperidin-1-yl)-3-oxopropanenitrile MDA-MB-231 C1420(0.98)  LDD2103  [3]
 LDCM0539  3-(4-Isopropylpiperazin-1-yl)-3-oxopropanenitrile MDA-MB-231 C1420(0.50)  LDD2132  [3]
 LDCM0498  BS-3668 MDA-MB-231 C1420(0.36)  LDD2091  [3]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C1420(4.94); C342(3.34); C628(0.35)  LDD1702  [3]
 LDCM0022  KB02 769-P C660(0.82); C1923(1.25); C1452(1.39); C1660(1.38)  LDD2246  [2]
 LDCM0023  KB03 MDA-MB-231 C1578(4.29); C456(1.49)  LDD1701  [3]
 LDCM0024  KB05 HMCB C1430(2.29); C660(2.08); C1923(2.61); C2010(3.05)  LDD3312  [2]
 LDCM0509  N-(4-bromo-3,5-dimethylphenyl)-2-nitroacetamide MDA-MB-231 C1491(0.86); C1420(0.93)  LDD2102  [3]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C1420(0.47)  LDD2121  [3]
 LDCM0497  Nucleophilic fragment 11b MDA-MB-231 C1420(1.16)  LDD2090  [3]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C1491(0.67); C1420(0.92)  LDD2092  [3]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C1491(0.65); C1420(1.06)  LDD2093  [3]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C1491(1.04); C1420(1.02)  LDD2094  [3]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C1491(0.82); C1420(1.05)  LDD2098  [3]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C1491(0.88); C1420(0.94)  LDD2099  [3]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C1491(0.72); C1420(0.92)  LDD2100  [3]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C1420(0.93)  LDD2101  [3]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C1491(1.32); C1420(1.31); C382(1.36)  LDD2105  [3]
 LDCM0513  Nucleophilic fragment 19b MDA-MB-231 C1420(0.76)  LDD2106  [3]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C1491(1.09); C1420(0.94); C824(0.77)  LDD2107  [3]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C1491(0.93); C1420(0.72)  LDD2108  [3]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C1491(0.98); C1420(0.68); C382(0.58)  LDD2109  [3]
 LDCM0517  Nucleophilic fragment 21b MDA-MB-231 C1420(1.24)  LDD2110  [3]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C1420(1.04)  LDD2111  [3]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C1491(0.72); C1420(0.62)  LDD2114  [3]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C1420(0.65)  LDD2115  [3]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C1420(0.77)  LDD2118  [3]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C1420(2.35)  LDD2119  [3]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C1491(0.89); C1420(0.82)  LDD2120  [3]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C1420(0.47); C824(0.64)  LDD2122  [3]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C1420(0.91)  LDD2123  [3]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C1491(1.08); C1420(0.74); C824(1.05)  LDD2125  [3]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C1491(2.14); C1420(1.13)  LDD2127  [3]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C1491(0.88); C1420(0.64)  LDD2128  [3]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C1491(0.97); C1420(0.97)  LDD2129  [3]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C1420(0.63)  LDD2133  [3]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C824(0.46)  LDD2134  [3]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C1491(1.00); C1420(1.13)  LDD2135  [3]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C1420(1.13); C382(1.08); C824(0.88)  LDD2136  [3]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C1491(1.36); C1420(1.04); C382(1.18); C824(0.83)  LDD2137  [3]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C1420(1.26)  LDD1700  [3]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C1491(0.68); C1420(0.71); C824(1.30)  LDD2140  [3]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C1491(1.17); C1420(0.76)  LDD2141  [3]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C1491(1.01); C1420(0.95); C824(1.04)  LDD2143  [3]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C1420(2.30)  LDD2144  [3]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C1420(0.83)  LDD2146  [3]
 LDCM0553  Nucleophilic fragment 6b MDA-MB-231 C1491(3.37); C1420(2.41)  LDD2147  [3]
 LDCM0555  Nucleophilic fragment 7b MDA-MB-231 C1420(0.48)  LDD2149  [3]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C1420(0.36)  LDD2150  [3]
 LDCM0559  Nucleophilic fragment 9b MDA-MB-231 C1420(1.41)  LDD2153  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Titin (TTN) CAMK Ser/Thr protein kinase family Q8WZ42
Immunoglobulin
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Myosin-binding protein C, slow-type (MYBPC1) MyBP family Q00872
Myomesin-1 (MYOM1) . P52179
Myomesin-2 (MYOM2) . P54296
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Gamma-sarcoglycan (SGCG) Sarcoglycan beta/delta/gamma/zeta family Q13326
Nebulin (NEB) . P20929

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
5 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264