Details of the Target

General Information of Target

Target ID LDTP01254
Target Name WW domain-binding protein 4 (WBP4)
Gene Name WBP4
Gene ID 11193
Synonyms
FBP21; FNBP21; WW domain-binding protein 4; WBP-4; Formin-binding protein 21; WW domain-containing-binding protein 4
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MADYWKSQPKKFCDYCKCWIADNRPSVEFHERGKNHKENVAKRISEIKQKSLDKAKEEEK
ASKEFAAMEAAALKAYQEDLKRLGLESEILEPSITPVTSTIPPTSTSNQQKEKKEKKKRK
KDPSKGRWVEGITSEGYHYYYDLISGASQWEKPEGFQGDLKKTAVKTVWVEGLSEDGFTY
YYNTETGESRWEKPDDFIPHTSDLPSSKVNENSLGTLDESKSSDSHSDSDGEQEAEEGGV
STETEKPKIKFKEKNKNSDGGSDPETQKEKSIQKQNSLGSNEEKSKTLKKSNPYGEWQEI
KQEVESHEEVDLELPSTENEYVSTSEADGGGEPKVVFKEKTVTSLGVMADGVAPVFKKRR
TENGKSRNLRQRGDDQ
Target Bioclass
Other
Subcellular location
Nucleus
Function Involved in pre-mRNA splicing as a component of the spliceosome. May play a role in cross-intron bridging of U1 and U2 snRNPs in the mammalian A complex.
Uniprot ID
O75554
Ensemble ID
ENST00000379487.5
HGNC ID
HGNC:12739

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCC1143 SNV: p.K113R .
HCT15 SNV: p.A21V .
HEC1B SNV: p.T106P .
HUPT3 Deletion: p.D122IfsTer8 .
JURKAT SNV: p.A41T .
SUPT1 Deletion: p.K162RfsTer4 DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C18(1.65)  LDD0532  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [2]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [2]
HHS-482
 Probe Info  		Y137(0.50)  LDD2239  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HCT 116 C18(1.65)  LDD0532  [1]
 LDCM0216  AC100 HCT 116 C18(0.97)  LDD0533  [1]
 LDCM0217  AC101 HCT 116 C18(1.19)  LDD0534  [1]
 LDCM0218  AC102 HCT 116 C18(1.33)  LDD0535  [1]
 LDCM0219  AC103 HCT 116 C18(1.11)  LDD0536  [1]
 LDCM0220  AC104 HCT 116 C18(1.27)  LDD0537  [1]
 LDCM0221  AC105 HCT 116 C18(1.05)  LDD0538  [1]
 LDCM0222  AC106 HCT 116 C18(1.38)  LDD0539  [1]
 LDCM0223  AC107 HCT 116 C18(1.08)  LDD0540  [1]
 LDCM0224  AC108 HCT 116 C18(1.29)  LDD0541  [1]
 LDCM0225  AC109 HCT 116 C18(1.15)  LDD0542  [1]
 LDCM0226  AC11 HCT 116 C18(1.14)  LDD0543  [1]
 LDCM0227  AC110 HCT 116 C18(1.51)  LDD0544  [1]
 LDCM0228  AC111 HCT 116 C18(1.16)  LDD0545  [1]
 LDCM0229  AC112 HCT 116 C18(1.17)  LDD0546  [1]
 LDCM0230  AC113 HCT 116 C18(0.92)  LDD0547  [1]
 LDCM0231  AC114 HCT 116 C18(0.85)  LDD0548  [1]
 LDCM0232  AC115 HCT 116 C18(0.91)  LDD0549  [1]
 LDCM0233  AC116 HCT 116 C18(0.96)  LDD0550  [1]
 LDCM0234  AC117 HCT 116 C18(0.84)  LDD0551  [1]
 LDCM0235  AC118 HCT 116 C18(0.87)  LDD0552  [1]
 LDCM0236  AC119 HCT 116 C18(0.93)  LDD0553  [1]
 LDCM0237  AC12 HCT 116 C18(1.37)  LDD0554  [1]
 LDCM0238  AC120 HCT 116 C18(0.97)  LDD0555  [1]
 LDCM0239  AC121 HCT 116 C18(0.77)  LDD0556  [1]
 LDCM0240  AC122 HCT 116 C18(0.98)  LDD0557  [1]
 LDCM0241  AC123 HCT 116 C18(0.79)  LDD0558  [1]
 LDCM0242  AC124 HCT 116 C18(0.94)  LDD0559  [1]
 LDCM0243  AC125 HCT 116 C18(1.05)  LDD0560  [1]
 LDCM0244  AC126 HCT 116 C18(1.13)  LDD0561  [1]
 LDCM0245  AC127 HCT 116 C18(0.95)  LDD0562  [1]
 LDCM0259  AC14 HCT 116 C18(1.34)  LDD0576  [1]
 LDCM0270  AC15 HCT 116 C18(1.34)  LDD0587  [1]
 LDCM0285  AC25 HCT 116 C18(0.94)  LDD0602  [1]
 LDCM0286  AC26 HCT 116 C18(0.93)  LDD0603  [1]
 LDCM0287  AC27 HCT 116 C18(1.13)  LDD0604  [1]
 LDCM0288  AC28 HCT 116 C18(0.93)  LDD0605  [1]
 LDCM0289  AC29 HCT 116 C18(1.02)  LDD0606  [1]
 LDCM0291  AC30 HCT 116 C18(0.78)  LDD0608  [1]
 LDCM0292  AC31 HCT 116 C18(1.16)  LDD0609  [1]
 LDCM0293  AC32 HCT 116 C18(1.39)  LDD0610  [1]
 LDCM0294  AC33 HCT 116 C18(1.03)  LDD0611  [1]
 LDCM0295  AC34 HCT 116 C18(1.19)  LDD0612  [1]
 LDCM0308  AC46 HCT 116 C18(1.16)  LDD0625  [1]
 LDCM0309  AC47 HCT 116 C18(0.98)  LDD0626  [1]
 LDCM0310  AC48 HCT 116 C18(0.93)  LDD0627  [1]
 LDCM0311  AC49 HCT 116 C18(0.95)  LDD0628  [1]
 LDCM0313  AC50 HCT 116 C18(0.91)  LDD0630  [1]
 LDCM0314  AC51 HCT 116 C18(0.84)  LDD0631  [1]
 LDCM0315  AC52 HCT 116 C18(0.98)  LDD0632  [1]
 LDCM0316  AC53 HCT 116 C18(0.85)  LDD0633  [1]
 LDCM0317  AC54 HCT 116 C18(1.03)  LDD0634  [1]
 LDCM0318  AC55 HCT 116 C18(1.23)  LDD0635  [1]
 LDCM0319  AC56 HCT 116 C18(0.78)  LDD0636  [1]
 LDCM0320  AC57 HCT 116 C18(0.72)  LDD0637  [1]
 LDCM0321  AC58 HCT 116 C18(1.13)  LDD0638  [1]
 LDCM0322  AC59 HCT 116 C18(1.05)  LDD0639  [1]
 LDCM0323  AC6 HCT 116 C18(1.23)  LDD0640  [1]
 LDCM0324  AC60 HCT 116 C18(1.00)  LDD0641  [1]
 LDCM0325  AC61 HCT 116 C18(0.88)  LDD0642  [1]
 LDCM0326  AC62 HCT 116 C18(0.90)  LDD0643  [1]
 LDCM0327  AC63 HCT 116 C18(1.05)  LDD0644  [1]
 LDCM0328  AC64 HCT 116 C18(1.00)  LDD0645  [1]
 LDCM0329  AC65 HCT 116 C18(0.97)  LDD0646  [1]
 LDCM0330  AC66 HCT 116 C18(1.14)  LDD0647  [1]
 LDCM0331  AC67 HCT 116 C18(0.85)  LDD0648  [1]
 LDCM0334  AC7 HCT 116 C18(1.38)  LDD0651  [1]
 LDCM0345  AC8 HCT 116 C18(1.38)  LDD0662  [1]
 LDCM0349  AC83 HCT 116 C18(1.50)  LDD0666  [1]
 LDCM0350  AC84 HCT 116 C18(1.41)  LDD0667  [1]
 LDCM0351  AC85 HCT 116 C18(1.13)  LDD0668  [1]
 LDCM0352  AC86 HCT 116 C18(1.27)  LDD0669  [1]
 LDCM0353  AC87 HCT 116 C18(1.33)  LDD0670  [1]
 LDCM0354  AC88 HCT 116 C18(1.20)  LDD0671  [1]
 LDCM0355  AC89 HCT 116 C18(1.30)  LDD0672  [1]
 LDCM0357  AC90 HCT 116 C18(0.99)  LDD0674  [1]
 LDCM0358  AC91 HCT 116 C18(1.76)  LDD0675  [1]
 LDCM0359  AC92 HCT 116 C18(1.88)  LDD0676  [1]
 LDCM0360  AC93 HCT 116 C18(1.54)  LDD0677  [1]
 LDCM0361  AC94 HCT 116 C18(1.51)  LDD0678  [1]
 LDCM0362  AC95 HCT 116 C18(1.03)  LDD0679  [1]
 LDCM0363  AC96 HCT 116 C18(1.41)  LDD0680  [1]
 LDCM0364  AC97 HCT 116 C18(1.43)  LDD0681  [1]
 LDCM0365  AC98 HCT 116 C18(1.47)  LDD0682  [1]
 LDCM0366  AC99 HCT 116 C18(1.05)  LDD0683  [1]
 LDCM0248  AKOS034007472 HCT 116 C18(1.39)  LDD0565  [1]
 LDCM0356  AKOS034007680 HCT 116 C18(1.19)  LDD0673  [1]
 LDCM0275  AKOS034007705 HCT 116 C18(1.16)  LDD0592  [1]
 LDCM0020  ARS-1620 HCC44 C18(1.07)  LDD2171  [1]
 LDCM0370  CL101 HCT 116 C18(1.29)  LDD0687  [1]
 LDCM0371  CL102 HCT 116 C18(1.04)  LDD0688  [1]
 LDCM0372  CL103 HCT 116 C18(1.22)  LDD0689  [1]
 LDCM0373  CL104 HCT 116 C18(1.23)  LDD0690  [1]
 LDCM0382  CL112 HCT 116 C18(0.99)  LDD0699  [1]
 LDCM0383  CL113 HCT 116 C18(1.05)  LDD0700  [1]
 LDCM0384  CL114 HCT 116 C18(1.29)  LDD0701  [1]
 LDCM0385  CL115 HCT 116 C18(1.39)  LDD0702  [1]
 LDCM0386  CL116 HCT 116 C18(1.39)  LDD0703  [1]
 LDCM0392  CL121 HCT 116 C18(1.10)  LDD0709  [1]
 LDCM0393  CL122 HCT 116 C18(1.09)  LDD0710  [1]
 LDCM0394  CL123 HCT 116 C18(0.97)  LDD0711  [1]
 LDCM0395  CL124 HCT 116 C18(1.02)  LDD0712  [1]
 LDCM0396  CL125 HCT 116 C18(0.91)  LDD0713  [1]
 LDCM0397  CL126 HCT 116 C18(1.27)  LDD0714  [1]
 LDCM0398  CL127 HCT 116 C18(1.14)  LDD0715  [1]
 LDCM0399  CL128 HCT 116 C18(0.86)  LDD0716  [1]
 LDCM0403  CL16 HCT 116 C18(0.87)  LDD0720  [1]
 LDCM0404  CL17 HCT 116 C18(1.05)  LDD0721  [1]
 LDCM0405  CL18 HCT 116 C18(1.41)  LDD0722  [1]
 LDCM0406  CL19 HCT 116 C18(1.25)  LDD0723  [1]
 LDCM0408  CL20 HCT 116 C18(1.03)  LDD0725  [1]
 LDCM0409  CL21 HCT 116 C18(1.10)  LDD0726  [1]
 LDCM0410  CL22 HCT 116 C18(1.13)  LDD0727  [1]
 LDCM0411  CL23 HCT 116 C18(1.28)  LDD0728  [1]
 LDCM0412  CL24 HCT 116 C18(1.31)  LDD0729  [1]
 LDCM0413  CL25 HCT 116 C18(0.87)  LDD0730  [1]
 LDCM0414  CL26 HCT 116 C18(1.15)  LDD0731  [1]
 LDCM0415  CL27 HCT 116 C18(1.07)  LDD0732  [1]
 LDCM0416  CL28 HCT 116 C18(0.90)  LDD0733  [1]
 LDCM0417  CL29 HCT 116 C18(1.24)  LDD0734  [1]
 LDCM0419  CL30 HCT 116 C18(1.13)  LDD0736  [1]
 LDCM0022  KB02 HEK-293T C18(0.97)  LDD1492  [4]
 LDCM0023  KB03 HEK-293T C18(0.90)  LDD1497  [4]
 LDCM0024  KB05 MKN45 C18(1.24)  LDD3332  [5]
 LDCM0021  THZ1 HCT 116 C18(1.07)  LDD2173  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
U5 small nuclear ribonucleoprotein 200 kDa helicase (SNRNP200) Helicase family O75643
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Huntingtin (HTT) Huntingtin family P42858

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840