Details of the Target

General Information of Target

Target ID LDTP00216
Target Name Amyloid beta precursor protein binding family B member 1 (APBB1)
Gene Name APBB1
Gene ID 322
Synonyms
FE65; RIR; Amyloid beta precursor protein binding family B member 1; Amyloid-beta A4 precursor protein-binding family B member 1; Protein Fe65
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSVPSSLSQSAINANSHGGPALSLPLPLHAAHNQLLNAKLQATAVGPKDLRSAMGEGGGP
EPGPANAKWLKEGQNQLRRAATAHRDQNRNVTLTLAEEASQEPEMAPLGPKGLIHLYSEL
ELSAHNAANRGLRGPGLIISTQEQGPDEGEEKAAGEAEEEEEDDDDEEEEEDLSSPPGLP
EPLESVEAPPRPQALTDGPREHSKSASLLFGMRNSAASDEDSSWATLSQGSPSYGSPEDT
DSFWNPNAFETDSDLPAGWMRVQDTSGTYYWHIPTGTTQWEPPGRASPSQGSSPQEESQL
TWTGFAHGEGFEDGEFWKDEPSDEAPMELGLKEPEEGTLTFPAQSLSPEPLPQEEEKLPP
RNTNPGIKCFAVRSLGWVEMTEEELAPGRSSVAVNNCIRQLSYHKNNLHDPMSGGWGEGK
DLLLQLEDETLKLVEPQSQALLHAQPIISIRVWGVGRDSGRERDFAYVARDKLTQMLKCH
VFRCEAPAKNIATSLHEICSKIMAERRNARCLVNGLSLDHSKLVDVPFQVEFPAPKNELV
QKFQVYYLGNVPVAKPVGVDVINGALESVLSSSSREQWTPSHVSVAPATLTILHQQTEAV
LGECRVRFLSFLAVGRDVHTFAFIMAAGPASFCCHMFWCEPNAASLSEAVQAACMLRYQK
CLDARSQASTSCLPAPPAESVARRVGWTVRRGVQSLWGSLKPKRLGAHTP
Target Bioclass
Other
Subcellular location
Cell membrane
Function
Transcription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its transcription activation activity. Functions in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s). Involved in hippocampal neurite branching and neuromuscular junction formation, as a result plays a role in spatial memory functioning. Plays a role in the maintenance of lens transparency. May play a role in muscle cell strength. Acts as a molecular adapter that functions in neurite outgrowth by activating the RAC1-ARF6 axis upon insulin treatment.
Uniprot ID
O00213
Ensemble ID
ENST00000299402.10
HGNC ID
HGNC:581

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A549 SNV: p.G59W .
AN3CA SNV: p.E162D .
HCC1395 SNV: p.E162K; p.A554T .
HCT15 SNV: p.Y117H .
HSC3 SNV: p.P360S .
JURKAT Deletion: p.Q295KfsTer37; p.E531DfsTer29 .
MCAS SNV: p.F532S .
MCC13 SNV: p.M327I .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		13.12  LDD0402  [1]
HHS-475
 Probe Info  		Y547(0.39); Y546(0.85)  LDD0264  [2]
DBIA
 Probe Info  		C397(2.87)  LDD0080  [3]
IA-alkyne
 Probe Info  		C397(0.00); C672(0.00)  LDD0165  [4]
IPM
 Probe Info  		N.A.  LDD0005  [5]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [6]
AOyne
 Probe Info  		13.60  LDD0443  [7]
HHS-465
 Probe Info  		Y546(0.00); K555(0.00); Y547(0.00)  LDD2240  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C672(1.06)  LDD1507  [9]
 LDCM0237  AC12 HEK-293T C672(1.13); C499(1.00)  LDD1510  [9]
 LDCM0259  AC14 HEK-293T C672(1.08); C499(1.16)  LDD1512  [9]
 LDCM0276  AC17 HEK-293T C672(1.02)  LDD1515  [9]
 LDCM0280  AC20 HEK-293T C672(1.11); C499(0.93)  LDD1519  [9]
 LDCM0281  AC21 HEK-293T C499(1.12); C511(0.98)  LDD1520  [9]
 LDCM0282  AC22 HEK-293T C672(0.99); C499(1.27)  LDD1521  [9]
 LDCM0284  AC24 HEK-293T C672(0.85); C511(1.01)  LDD1523  [9]
 LDCM0285  AC25 HEK-293T C672(0.99)  LDD1524  [9]
 LDCM0288  AC28 HEK-293T C672(0.92); C499(0.96)  LDD1527  [9]
 LDCM0289  AC29 HEK-293T C499(1.11); C511(1.15)  LDD1528  [9]
 LDCM0291  AC30 HEK-293T C672(0.94); C499(1.11)  LDD1530  [9]
 LDCM0293  AC32 HEK-293T C672(0.84); C511(1.04)  LDD1532  [9]
 LDCM0294  AC33 HEK-293T C672(1.11)  LDD1533  [9]
 LDCM0297  AC36 HEK-293T C672(1.02); C499(1.10)  LDD1536  [9]
 LDCM0298  AC37 HEK-293T C499(1.15); C511(0.95)  LDD1537  [9]
 LDCM0299  AC38 HEK-293T C672(1.04); C499(1.24)  LDD1538  [9]
 LDCM0301  AC4 HEK-293T C672(0.88); C499(0.96)  LDD1540  [9]
 LDCM0302  AC40 HEK-293T C672(1.10); C511(1.07)  LDD1541  [9]
 LDCM0303  AC41 HEK-293T C672(1.05)  LDD1542  [9]
 LDCM0306  AC44 HEK-293T C672(0.86); C499(0.95)  LDD1545  [9]
 LDCM0307  AC45 HEK-293T C499(1.05); C511(1.06)  LDD1546  [9]
 LDCM0308  AC46 HEK-293T C672(0.92); C499(1.21)  LDD1547  [9]
 LDCM0310  AC48 HEK-293T C672(0.97); C511(1.10)  LDD1549  [9]
 LDCM0311  AC49 HEK-293T C672(1.00)  LDD1550  [9]
 LDCM0312  AC5 HEK-293T C499(1.07); C511(0.99)  LDD1551  [9]
 LDCM0315  AC52 HEK-293T C672(0.82); C499(1.04)  LDD1554  [9]
 LDCM0316  AC53 HEK-293T C499(1.11); C511(1.11)  LDD1555  [9]
 LDCM0317  AC54 HEK-293T C672(1.02); C499(1.07)  LDD1556  [9]
 LDCM0319  AC56 HEK-293T C672(1.01); C511(1.03)  LDD1558  [9]
 LDCM0320  AC57 HEK-293T C672(0.98)  LDD1559  [9]
 LDCM0323  AC6 HEK-293T C672(1.00); C499(1.20)  LDD1562  [9]
 LDCM0324  AC60 HEK-293T C672(1.12); C499(0.92)  LDD1563  [9]
 LDCM0325  AC61 HEK-293T C499(1.08); C511(1.07)  LDD1564  [9]
 LDCM0326  AC62 HEK-293T C672(1.01); C499(1.20)  LDD1565  [9]
 LDCM0328  AC64 HEK-293T C672(0.94); C511(0.98)  LDD1567  [9]
 LDCM0345  AC8 HEK-293T C672(0.98); C511(0.91)  LDD1569  [9]
 LDCM0248  AKOS034007472 HEK-293T C499(1.14); C511(1.05)  LDD1511  [9]
 LDCM0356  AKOS034007680 HEK-293T C672(1.00)  LDD1570  [9]
 LDCM0275  AKOS034007705 HEK-293T C672(1.02); C511(1.03)  LDD1514  [9]
 LDCM0367  CL1 HEK-293T C672(1.10); C499(1.07)  LDD1571  [9]
 LDCM0368  CL10 HEK-293T C672(0.98); C499(0.88)  LDD1572  [9]
 LDCM0369  CL100 HEK-293T C672(1.09); C499(1.10); C511(1.00)  LDD1573  [9]
 LDCM0370  CL101 HEK-293T C672(1.19); C499(0.92)  LDD1574  [9]
 LDCM0371  CL102 HEK-293T C672(0.82)  LDD1575  [9]
 LDCM0372  CL103 HEK-293T C511(1.03)  LDD1576  [9]
 LDCM0373  CL104 HEK-293T C672(1.46); C499(0.96); C511(1.05)  LDD1577  [9]
 LDCM0374  CL105 HEK-293T C672(1.03); C499(1.00)  LDD1578  [9]
 LDCM0375  CL106 HEK-293T C672(1.02)  LDD1579  [9]
 LDCM0376  CL107 HEK-293T C511(1.01)  LDD1580  [9]
 LDCM0377  CL108 HEK-293T C672(1.57); C499(1.01); C511(0.92)  LDD1581  [9]
 LDCM0378  CL109 HEK-293T C672(0.95); C499(1.12)  LDD1582  [9]
 LDCM0380  CL110 HEK-293T C672(0.93)  LDD1584  [9]
 LDCM0381  CL111 HEK-293T C511(1.19)  LDD1585  [9]
 LDCM0382  CL112 HEK-293T C672(1.46); C499(0.97); C511(0.87)  LDD1586  [9]
 LDCM0383  CL113 HEK-293T C672(1.20); C499(1.10)  LDD1587  [9]
 LDCM0384  CL114 HEK-293T C672(0.94)  LDD1588  [9]
 LDCM0385  CL115 HEK-293T C511(1.10)  LDD1589  [9]
 LDCM0386  CL116 HEK-293T C672(1.70); C499(0.98); C511(1.07)  LDD1590  [9]
 LDCM0387  CL117 HEK-293T C672(1.07); C499(0.96)  LDD1591  [9]
 LDCM0388  CL118 HEK-293T C672(0.89)  LDD1592  [9]
 LDCM0389  CL119 HEK-293T C511(1.10)  LDD1593  [9]
 LDCM0390  CL12 HEK-293T C672(1.16); C511(1.18)  LDD1594  [9]
 LDCM0391  CL120 HEK-293T C672(1.32); C499(0.91); C511(1.09)  LDD1595  [9]
 LDCM0392  CL121 HEK-293T C672(1.07); C499(1.06)  LDD1596  [9]
 LDCM0393  CL122 HEK-293T C672(1.05)  LDD1597  [9]
 LDCM0394  CL123 HEK-293T C511(1.16)  LDD1598  [9]
 LDCM0395  CL124 HEK-293T C672(1.10); C499(0.98); C511(0.91)  LDD1599  [9]
 LDCM0396  CL125 HEK-293T C672(1.16); C499(0.98)  LDD1600  [9]
 LDCM0397  CL126 HEK-293T C672(1.01)  LDD1601  [9]
 LDCM0398  CL127 HEK-293T C511(0.90)  LDD1602  [9]
 LDCM0399  CL128 HEK-293T C672(1.11); C499(0.96); C511(0.91)  LDD1603  [9]
 LDCM0400  CL13 HEK-293T C672(1.08); C499(0.95)  LDD1604  [9]
 LDCM0401  CL14 HEK-293T C672(1.24)  LDD1605  [9]
 LDCM0402  CL15 HEK-293T C511(1.20)  LDD1606  [9]
 LDCM0403  CL16 HEK-293T C672(1.72); C499(1.17); C511(1.05)  LDD1607  [9]
 LDCM0404  CL17 HEK-293T C672(1.02)  LDD1608  [9]
 LDCM0407  CL2 HEK-293T C672(0.98)  LDD1611  [9]
 LDCM0408  CL20 HEK-293T C672(1.15); C499(1.13)  LDD1612  [9]
 LDCM0409  CL21 HEK-293T C499(0.98); C511(1.39)  LDD1613  [9]
 LDCM0410  CL22 HEK-293T C672(1.14); C499(1.20)  LDD1614  [9]
 LDCM0412  CL24 HEK-293T C672(1.03); C511(1.13)  LDD1616  [9]
 LDCM0413  CL25 HEK-293T C672(1.12); C499(0.90)  LDD1617  [9]
 LDCM0414  CL26 HEK-293T C672(0.87)  LDD1618  [9]
 LDCM0415  CL27 HEK-293T C511(1.12)  LDD1619  [9]
 LDCM0416  CL28 HEK-293T C672(1.22); C499(0.99); C511(0.97)  LDD1620  [9]
 LDCM0417  CL29 HEK-293T C672(0.96)  LDD1621  [9]
 LDCM0418  CL3 HEK-293T C511(1.08)  LDD1622  [9]
 LDCM0421  CL32 HEK-293T C672(0.98); C499(1.04)  LDD1625  [9]
 LDCM0422  CL33 HEK-293T C499(0.92); C511(1.37)  LDD1626  [9]
 LDCM0423  CL34 HEK-293T C672(1.04); C499(1.18)  LDD1627  [9]
 LDCM0425  CL36 HEK-293T C672(1.03); C511(1.15)  LDD1629  [9]
 LDCM0426  CL37 HEK-293T C672(1.11); C499(1.05)  LDD1630  [9]
 LDCM0428  CL39 HEK-293T C511(1.21)  LDD1632  [9]
 LDCM0429  CL4 HEK-293T C672(1.38); C499(0.81); C511(1.00)  LDD1633  [9]
 LDCM0430  CL40 HEK-293T C672(1.11); C499(1.11); C511(1.02)  LDD1634  [9]
 LDCM0431  CL41 HEK-293T C672(0.91)  LDD1635  [9]
 LDCM0434  CL44 HEK-293T C672(0.97); C499(0.92)  LDD1638  [9]
 LDCM0435  CL45 HEK-293T C499(0.71); C511(1.21)  LDD1639  [9]
 LDCM0436  CL46 HEK-293T C672(0.99); C499(1.21)  LDD1640  [9]
 LDCM0438  CL48 HEK-293T C672(1.18); C511(1.20)  LDD1642  [9]
 LDCM0439  CL49 HEK-293T C672(1.00); C499(1.13)  LDD1643  [9]
 LDCM0440  CL5 HEK-293T C672(1.07)  LDD1644  [9]
 LDCM0441  CL50 HEK-293T C672(0.93)  LDD1645  [9]
 LDCM0443  CL52 HEK-293T C672(0.97); C499(1.13); C511(1.13)  LDD1646  [9]
 LDCM0444  CL53 HEK-293T C672(1.03)  LDD1647  [9]
 LDCM0447  CL56 HEK-293T C672(0.94); C499(1.07)  LDD1650  [9]
 LDCM0448  CL57 HEK-293T C499(0.96); C511(1.25)  LDD1651  [9]
 LDCM0449  CL58 HEK-293T C672(1.15); C499(1.08)  LDD1652  [9]
 LDCM0452  CL60 HEK-293T C672(1.02); C511(1.17)  LDD1655  [9]
 LDCM0453  CL61 HEK-293T C672(1.12); C499(0.91)  LDD1656  [9]
 LDCM0454  CL62 HEK-293T C672(1.06)  LDD1657  [9]
 LDCM0455  CL63 HEK-293T C511(1.05)  LDD1658  [9]
 LDCM0456  CL64 HEK-293T C672(1.18); C499(1.14); C511(1.12)  LDD1659  [9]
 LDCM0457  CL65 HEK-293T C672(0.97)  LDD1660  [9]
 LDCM0460  CL68 HEK-293T C672(0.85); C499(1.10)  LDD1663  [9]
 LDCM0461  CL69 HEK-293T C499(1.03); C511(1.09)  LDD1664  [9]
 LDCM0463  CL70 HEK-293T C672(1.23); C499(1.18)  LDD1666  [9]
 LDCM0465  CL72 HEK-293T C672(1.06); C511(1.09)  LDD1668  [9]
 LDCM0466  CL73 HEK-293T C672(1.15); C499(0.89)  LDD1669  [9]
 LDCM0467  CL74 HEK-293T C672(0.99)  LDD1670  [9]
 LDCM0469  CL76 HEK-293T C672(1.46); C499(1.02); C511(1.05)  LDD1672  [9]
 LDCM0470  CL77 HEK-293T C672(1.01)  LDD1673  [9]
 LDCM0473  CL8 HEK-293T C672(1.09); C499(0.72)  LDD1676  [9]
 LDCM0474  CL80 HEK-293T C672(0.99); C499(1.00)  LDD1677  [9]
 LDCM0475  CL81 HEK-293T C499(1.00); C511(1.12)  LDD1678  [9]
 LDCM0476  CL82 HEK-293T C672(1.07); C499(1.12)  LDD1679  [9]
 LDCM0478  CL84 HEK-293T C672(1.02); C511(1.14)  LDD1681  [9]
 LDCM0479  CL85 HEK-293T C672(1.14); C499(0.89)  LDD1682  [9]
 LDCM0480  CL86 HEK-293T C672(0.90)  LDD1683  [9]
 LDCM0481  CL87 HEK-293T C511(0.98)  LDD1684  [9]
 LDCM0482  CL88 HEK-293T C672(1.27); C499(0.87); C511(1.01)  LDD1685  [9]
 LDCM0483  CL89 HEK-293T C672(1.13)  LDD1686  [9]
 LDCM0484  CL9 HEK-293T C499(1.07); C511(1.18)  LDD1687  [9]
 LDCM0487  CL92 HEK-293T C672(1.05); C499(0.88)  LDD1690  [9]
 LDCM0488  CL93 HEK-293T C499(1.12); C511(1.13)  LDD1691  [9]
 LDCM0489  CL94 HEK-293T C672(1.22); C499(1.14)  LDD1692  [9]
 LDCM0491  CL96 HEK-293T C672(1.10); C511(1.12)  LDD1694  [9]
 LDCM0492  CL97 HEK-293T C672(1.03); C499(1.21)  LDD1695  [9]
 LDCM0493  CL98 HEK-293T C672(1.19)  LDD1696  [9]
 LDCM0494  CL99 HEK-293T C511(1.10)  LDD1697  [9]
 LDCM0495  E2913 HEK-293T C511(1.09)  LDD1698  [9]
 LDCM0468  Fragment33 HEK-293T C511(1.03)  LDD1671  [9]
 LDCM0427  Fragment51 HEK-293T C672(1.34)  LDD1631  [9]
 LDCM0116  HHS-0101 DM93 Y547(0.39); Y546(0.85)  LDD0264  [2]
 LDCM0117  HHS-0201 DM93 Y546(0.93); Y547(1.03)  LDD0265  [2]
 LDCM0118  HHS-0301 DM93 Y546(0.61); Y547(0.61)  LDD0266  [2]
 LDCM0119  HHS-0401 DM93 Y547(0.83); Y546(0.84)  LDD0267  [2]
 LDCM0120  HHS-0701 DM93 Y547(0.92); Y546(0.97)  LDD0268  [2]
 LDCM0022  KB02 HCT 116 C397(2.87)  LDD0080  [3]
 LDCM0023  KB03 HCT 116 C397(2.88)  LDD0081  [3]
 LDCM0024  KB05 HCT 116 C397(1.63)  LDD0082  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
F-box DNA helicase 1 (FBH1) Helicase family Q8NFZ0
Histone acetyltransferase KAT5 (KAT5) MYST (SAS/MOZ) family Q92993
Epidermal growth factor receptor (EGFR) Tyr protein kinase family P00533
Receptor tyrosine-protein kinase erbB-2 (ERBB2) Tyr protein kinase family P04626
ADP-ribosylation factor 6 (ARF6) Arf family P62330
Septin-6 (SEPTIN6) Septin GTPase family Q14141
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Amyloid-beta precursor protein (APP) APP family P05067
Prolow-density lipoprotein receptor-related protein 1 (LRP1) LDLR family Q07954
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Transcription factor SOX-14 (SOX14) . O95416
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
U6 snRNA-associated Sm-like protein LSm1 (LSM1) SnRNP Sm proteins family O15116

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
3 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
6 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
7 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
8 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
9 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402