Details of the Target

General Information of Target

Target ID LDTP00119
Target Name Tubulin beta 8B (TUBB8B)
Gene Name TUBB8B
Synonyms
Tubulin beta 8B
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MREIVLTQTGQCGNQIGAKFWEVISDEHAIDSAGTYHGDSHLQLERINVHHHEASGGRYV
PRAVLVDLEPGTMDSVHSGPFGQVFRPDNFISGQCGAGNNWAKGRYTEGAELTESVMDVV
RKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIINTFSILPSPKVSDTVV
EPYNATLSVHQLIENADETFCIDNEALYDICSRTLKLPTPTYGDLNHLVSATMSGVTTCL
RFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVAELTQQMFDAKNMM
AACDPRHGCYLTVAAIFRGRMPMREVDEQMFNIQDKNSSYFADWFPDNVKTAVCDIPPRG
LKMSATFIGNNAAIQELFTCVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
EYQQYQDATAEEEEDEEYAEEEVA
Target Bioclass
Other
Family
Tubulin family
Subcellular location
Cytoplasm, cytoskeleton
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Uniprot ID
A6NNZ2
Ensemble ID
ENST00000308911.9
HGNC ID
HGNC:24983
ChEMBL ID
CHEMBL3832942

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 17 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
OPA-S-S-alkyne
 Probe Info  		K252(2.04)  LDD3494  [1]
JZ128-DTB
 Probe Info  		C129(0.00); C127(0.00)  LDD0462  [2]
THZ1-DTB
 Probe Info  		C303(1.20); C127(1.08); C354(1.05); C129(1.02)  LDD0460  [2]
BTD
 Probe Info  		C12(0.85)  LDD2094  [3]
AHL-Pu-1
 Probe Info  		C129(2.47); C303(2.15)  LDD0168  [4]
HHS-475
 Probe Info  		Y281(0.79)  LDD0264  [5]
HHS-465
 Probe Info  		Y159(8.69); Y222(6.05); Y281(7.61)  LDD2237  [6]
DBIA
 Probe Info  		C127(1.03); C129(1.15); C127(0.98); C129(0.98)  LDD1492  [7]
5E-2FA
 Probe Info  		H137(0.00); H264(0.00); H227(0.00)  LDD2235  [8]
m-APA
 Probe Info  		H137(0.00); H264(0.00); H227(0.00)  LDD2231  [8]
IA-alkyne
 Probe Info  		C239(0.00); C354(0.00); C303(0.00); C127(0.00)  LDD0166  [9]
JW-RF-010
 Probe Info  		C303(0.00); C129(0.00); C354(0.00); C127(0.00)  LDD0026  [10]
NAIA_4
 Probe Info  		N.A.  LDD2226  [11]
ENE
 Probe Info  		C303(0.00); C354(0.00)  LDD0006  [12]
IPM
 Probe Info  		C303(0.00); C354(0.00); C127(0.00); C129(0.00)  LDD0147  [10]
VSF
 Probe Info  		C129(0.00); C127(0.00)  LDD0007  [12]
NAIA_5
 Probe Info  		C127(0.00); C239(0.00); C12(0.00); C303(0.00)  LDD2223  [11]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C129(2.47); C303(2.15)  LDD0168  [4]
 LDCM0630  CCW28-3 231MFP C239(1.48)  LDD2214  [13]
 LDCM0116  HHS-0101 DM93 Y281(0.79)  LDD0264  [5]
 LDCM0117  HHS-0201 DM93 Y281(0.80)  LDD0265  [5]
 LDCM0118  HHS-0301 DM93 Y281(0.85)  LDD0266  [5]
 LDCM0119  HHS-0401 DM93 Y281(0.91)  LDD0267  [5]
 LDCM0120  HHS-0701 DM93 Y281(0.76)  LDD0268  [5]
 LDCM0179  JZ128 PC-3 C129(0.00); C127(0.00)  LDD0462  [2]
 LDCM0022  KB02 HEK-293T C127(1.03); C129(1.15); C127(0.98); C129(0.98)  LDD1492  [7]
 LDCM0023  KB03 HEK-293T C127(0.99); C129(1.13); C127(0.94); C129(0.94)  LDD1497  [7]
 LDCM0024  KB05 HEK-293T C127(1.12); C129(1.13); C127(1.04); C129(1.04)  LDD1502  [7]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C12(0.85)  LDD2094  [3]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C12(1.17)  LDD2098  [3]
 LDCM0627  NUDT7-COV-1 HEK-293T C129(1.13)  LDD2206  [14]
 LDCM0628  OTUB2-COV-1 HEK-293T C12(15.00); C127(0.48); C129(0.48); C127(0.20)  LDD2207  [14]
 LDCM0021  THZ1 HeLa S3 C303(1.20); C127(1.08); C354(1.05); C129(1.02)  LDD0460  [2]

References

1 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
2 A Chemoproteomic Strategy for Direct and Proteome-Wide Covalent Inhibitor Target-Site Identification. J Am Chem Soc. 2019 Jan 9;141(1):191-203. doi: 10.1021/jacs.8b07911. Epub 2018 Dec 20.
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
5 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
6 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
7 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
8 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
9 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
10 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
11 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
12 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
13 Covalent Ligand Screening Uncovers a RNF4 E3 Ligase Recruiter for Targeted Protein Degradation Applications. ACS Chem Biol. 2019 Nov 15;14(11):2430-2440. doi: 10.1021/acschembio.8b01083. Epub 2019 May 13.
14 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.