Details of the Target

General Information of Target

Target ID LDTP14644
Target Name Zinc finger protein 28 (ZNF28)
Gene Name ZNF28
Gene ID 7576
Synonyms
KOX24; Zinc finger protein 28; Zinc finger protein KOX24
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MITSQGSVSFRDVTVGFTQEEWQHLDPAQRTLYRDVMLENYSHLVSVGYCIPKPEVILKL
EKGEEPWILEEKFPSQSHLELINTSRNYSIMKFNEFNKGGKCFCDEKHEIIHSEEEPSEY
NKNGNSFWLNEDLIWHQKIKNWEQSFEYNECGKAFPENSLFLVHKRGYTGQKTCKYTEHG
KTCDMSFFITHQQTHPRENHYGNECGENIFEESILLEHQSVYPFSQKLNLTPIQRTHSIN
NIIEYNECGTFFSEKLVLHLQQRTHTGEKPYECHECGKTFTQKSAHTRHQRTHTGGKPYE
CHECGKTFYKNSDLIKHQRIHTGERPYGCHECGKSFSEKSTLTQHQRTHTGEKPYECHEC
GKTFSFKSVLTVHQKTHTGEKPYECYACGKAFLRKSDLIKHQRIHTGEKPYECNECGKSF
SEKSTLTKHLRTHTGEKPYECIQCGKFFCYYSGFTEHLRRHTGEKPFGCNECGKTFRQKS
ALIVHQRTHIRQKPYGCNQCGKSFCVKSKLIAHHRTHTGEKPYECNVCGKSFYVKSKLTV
HQRIHLGRNPINVVNEGNYSG
Target Bioclass
Transcription factor
Family
Krueppel C2H2-type zinc-finger protein family
Subcellular location
Nucleus
Function May be involved in transcriptional regulation.
Uniprot ID
P17035
Ensemble ID
ENST00000438150.2
HGNC ID
HGNC:13073

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.Y248C
Substitution: p.T236I		 DBIA    Probe Info 
AGS SNV: p.L47Q DBIA    Probe Info 
COLO829 SNV: p.I572T DBIA    Probe Info 
IGR1 SNV: p.P382L DBIA    Probe Info 
IPC298 SNV: p.S46F DBIA    Probe Info 
JHH7 SNV: p.P466Q .
MCC13 SNV: p.L197F; p.S480L .
NCIH1666 SNV: p.G62E DBIA    Probe Info 
NCIH1793 SNV: p.G281V .
NCIH358 SNV: p.D305A DBIA    Probe Info 
RPMI8226 SNV: p.Q675P .
SKOV3 SNV: p.T518I DBIA    Probe Info 
SW1271 SNV: p.G529C .
TF1 SNV: p.E436Q DBIA    Probe Info 
TKKK SNV: p.E91K DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C217(25.54); C24(76.49)  LDD0209  [1]
IA-alkyne
 Probe Info  		C217(7.46)  LDD1704  [2]
CY-1
 Probe Info  		H677(0.00); H681(0.00); H685(0.00)  LDD0246  [3]
HHS-465
 Probe Info  		K353(0.00); K356(0.00); K633(0.00); K636(0.00)  LDD2240  [4]
HHS-482
 Probe Info  		Y607(1.28)  LDD2239  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0270  AC15 HEK-293T C217(1.00)  LDD1513  [6]
 LDCM0281  AC21 HEK-293T C226(1.17); C217(1.05)  LDD1520  [6]
 LDCM0283  AC23 HEK-293T C217(0.91)  LDD1522  [6]
 LDCM0284  AC24 HEK-293T C217(0.90)  LDD1523  [6]
 LDCM0289  AC29 HEK-293T C226(0.87); C217(0.97)  LDD1528  [6]
 LDCM0292  AC31 HEK-293T C217(1.05)  LDD1531  [6]
 LDCM0293  AC32 HEK-293T C217(0.88)  LDD1532  [6]
 LDCM0298  AC37 HEK-293T C226(0.86); C217(0.90)  LDD1537  [6]
 LDCM0300  AC39 HEK-293T C217(1.02)  LDD1539  [6]
 LDCM0302  AC40 HEK-293T C217(0.81)  LDD1541  [6]
 LDCM0307  AC45 HEK-293T C226(0.81); C217(1.14)  LDD1546  [6]
 LDCM0309  AC47 HEK-293T C217(1.06)  LDD1548  [6]
 LDCM0310  AC48 HEK-293T C217(0.90)  LDD1549  [6]
 LDCM0312  AC5 HEK-293T C226(0.83); C217(1.00)  LDD1551  [6]
 LDCM0316  AC53 HEK-293T C226(1.07); C217(1.16)  LDD1555  [6]
 LDCM0318  AC55 HEK-293T C217(0.82)  LDD1557  [6]
 LDCM0319  AC56 HEK-293T C217(0.91)  LDD1558  [6]
 LDCM0325  AC61 HEK-293T C226(1.02); C217(1.05)  LDD1564  [6]
 LDCM0327  AC63 HEK-293T C217(0.99)  LDD1566  [6]
 LDCM0328  AC64 HEK-293T C217(1.06)  LDD1567  [6]
 LDCM0334  AC7 HEK-293T C217(1.05)  LDD1568  [6]
 LDCM0345  AC8 HEK-293T C217(0.83)  LDD1569  [6]
 LDCM0248  AKOS034007472 HEK-293T C226(0.98); C217(1.12)  LDD1511  [6]
 LDCM0275  AKOS034007705 HEK-293T C217(0.85)  LDD1514  [6]
 LDCM0371  CL102 HEK-293T C226(1.10)  LDD1575  [6]
 LDCM0372  CL103 HEK-293T C226(0.94)  LDD1576  [6]
 LDCM0375  CL106 HEK-293T C226(0.85)  LDD1579  [6]
 LDCM0376  CL107 HEK-293T C226(0.85)  LDD1580  [6]
 LDCM0379  CL11 HEK-293T C217(1.02)  LDD1583  [6]
 LDCM0380  CL110 HEK-293T C226(0.86)  LDD1584  [6]
 LDCM0381  CL111 HEK-293T C226(0.99)  LDD1585  [6]
 LDCM0384  CL114 HEK-293T C226(0.99)  LDD1588  [6]
 LDCM0385  CL115 HEK-293T C226(0.90)  LDD1589  [6]
 LDCM0388  CL118 HEK-293T C226(0.92)  LDD1592  [6]
 LDCM0389  CL119 HEK-293T C226(0.90)  LDD1593  [6]
 LDCM0390  CL12 HEK-293T C217(0.91)  LDD1594  [6]
 LDCM0393  CL122 HEK-293T C226(0.84)  LDD1597  [6]
 LDCM0394  CL123 HEK-293T C226(0.99)  LDD1598  [6]
 LDCM0397  CL126 HEK-293T C226(0.98)  LDD1601  [6]
 LDCM0398  CL127 HEK-293T C226(1.04)  LDD1602  [6]
 LDCM0401  CL14 HEK-293T C226(0.97)  LDD1605  [6]
 LDCM0402  CL15 HEK-293T C226(0.92)  LDD1606  [6]
 LDCM0407  CL2 HEK-293T C226(1.05)  LDD1611  [6]
 LDCM0409  CL21 HEK-293T C226(0.99); C217(1.15)  LDD1613  [6]
 LDCM0411  CL23 HEK-293T C217(0.99)  LDD1615  [6]
 LDCM0412  CL24 HEK-293T C217(0.92)  LDD1616  [6]
 LDCM0414  CL26 HEK-293T C226(1.02)  LDD1618  [6]
 LDCM0415  CL27 HEK-293T C226(0.88)  LDD1619  [6]
 LDCM0418  CL3 HEK-293T C226(0.94)  LDD1622  [6]
 LDCM0422  CL33 HEK-293T C226(1.05); C217(1.09)  LDD1626  [6]
 LDCM0424  CL35 HEK-293T C217(1.06)  LDD1628  [6]
 LDCM0425  CL36 HEK-293T C217(1.12)  LDD1629  [6]
 LDCM0428  CL39 HEK-293T C226(1.02)  LDD1632  [6]
 LDCM0435  CL45 HEK-293T C226(1.11); C217(1.22)  LDD1639  [6]
 LDCM0437  CL47 HEK-293T C217(1.18)  LDD1641  [6]
 LDCM0438  CL48 HEK-293T C217(1.00)  LDD1642  [6]
 LDCM0441  CL50 HEK-293T C226(1.07)  LDD1645  [6]
 LDCM0448  CL57 HEK-293T C226(1.08); C217(1.10)  LDD1651  [6]
 LDCM0450  CL59 HEK-293T C217(0.92)  LDD1653  [6]
 LDCM0452  CL60 HEK-293T C217(0.88)  LDD1655  [6]
 LDCM0454  CL62 HEK-293T C226(0.93)  LDD1657  [6]
 LDCM0455  CL63 HEK-293T C226(1.10)  LDD1658  [6]
 LDCM0461  CL69 HEK-293T C226(1.00); C217(1.26)  LDD1664  [6]
 LDCM0464  CL71 HEK-293T C217(0.95)  LDD1667  [6]
 LDCM0465  CL72 HEK-293T C217(1.16)  LDD1668  [6]
 LDCM0467  CL74 HEK-293T C226(0.91)  LDD1670  [6]
 LDCM0475  CL81 HEK-293T C226(1.01); C217(1.41)  LDD1678  [6]
 LDCM0477  CL83 HEK-293T C217(0.98)  LDD1680  [6]
 LDCM0478  CL84 HEK-293T C217(1.13)  LDD1681  [6]
 LDCM0480  CL86 HEK-293T C226(0.86)  LDD1683  [6]
 LDCM0481  CL87 HEK-293T C226(1.03)  LDD1684  [6]
 LDCM0484  CL9 HEK-293T C226(1.07); C217(1.43)  LDD1687  [6]
 LDCM0488  CL93 HEK-293T C226(1.05); C217(1.14)  LDD1691  [6]
 LDCM0490  CL95 HEK-293T C217(1.03)  LDD1693  [6]
 LDCM0491  CL96 HEK-293T C217(0.93)  LDD1694  [6]
 LDCM0493  CL98 HEK-293T C226(0.87)  LDD1696  [6]
 LDCM0494  CL99 HEK-293T C226(0.97)  LDD1697  [6]
 LDCM0495  E2913 HEK-293T C226(0.85)  LDD1698  [6]
 LDCM0625  F8 Ramos C24(0.92)  LDD2187  [7]
 LDCM0573  Fragment11 Ramos C24(0.64)  LDD2190  [7]
 LDCM0575  Fragment13 Ramos C24(0.96)  LDD2192  [7]
 LDCM0576  Fragment14 Ramos C24(1.55)  LDD2193  [7]
 LDCM0580  Fragment21 Ramos C24(1.21)  LDD2195  [7]
 LDCM0582  Fragment23 Ramos C24(1.00)  LDD2196  [7]
 LDCM0578  Fragment27 Ramos C24(1.08)  LDD2197  [7]
 LDCM0586  Fragment28 Ramos C24(0.81)  LDD2198  [7]
 LDCM0588  Fragment30 Ramos C24(1.38)  LDD2199  [7]
 LDCM0589  Fragment31 Ramos C24(1.21)  LDD2200  [7]
 LDCM0590  Fragment32 Ramos C24(1.38)  LDD2201  [7]
 LDCM0468  Fragment33 HEK-293T C226(1.06)  LDD1671  [6]
 LDCM0596  Fragment38 Ramos C24(1.22)  LDD2203  [7]
 LDCM0566  Fragment4 Ramos C24(1.74)  LDD2184  [7]
 LDCM0427  Fragment51 HEK-293T C226(0.86)  LDD1631  [6]
 LDCM0610  Fragment52 Ramos C24(1.31)  LDD2204  [7]
 LDCM0614  Fragment56 Ramos C24(1.27)  LDD2205  [7]
 LDCM0569  Fragment7 Ramos C24(1.99)  LDD2186  [7]
 LDCM0022  KB02 T cell-activated C217(7.46)  LDD1704  [2]
 LDCM0023  KB03 Jurkat C217(25.54); C24(76.49)  LDD0209  [1]
 LDCM0024  KB05 HMCB C90(2.57)  LDD3312  [8]

References

1 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
2 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
3 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
4 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
5 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
7 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
8 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840