Details of the Target

General Information of Target

Target ID LDTP13602
Target Name Fizzy-related protein homolog (FZR1)
Gene Name FZR1
Gene ID 51343
Synonyms
CDH1; FYR; FZR; KIAA1242; Fizzy-related protein homolog; Fzr; CDC20-like protein 1; Cdh1/Hct1 homolog; hCDH1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAQGTLIRVTPEQPTHAVCVLGTLTQLDICSSAPEDCTSFSINASPGVVVDIAHGPPAKK
KSTGSSTWPLDPGVEVTLTMKVASGSTGDQKVQISYYGPKTPPVKALLYLTGVEISLCAD
ITRTGKVKPTRAVKDQRTWTWGPCGQGAILLVNCDRDNLESSAMDCEDDEVLDSEDLQDM
SLMTLSTKTPKDFFTNHTLVLHVARSEMDKVRVFQATRGKLSSKCSVVLGPKWPSHYLMV
PGGKHNMDFYVEALAFPDTDFPGLITLTISLLDTSNLELPEAVVFQDSVVFRVAPWIMTP
NTQPPQEVYACSIFENEDFLKSVTTLAMKAKCKLTICPEEENMDDQWMQDEMEIGYIQAP
HKTLPVVFDSPRNRGLKEFPIKRVMGPDFGYVTRGPQTGGISGLDSFGNLEVSPPVTVRG
KEYPLGRILFGDSCYPSNDSRQMHQALQDFLSAQQVQAPVKLYSDWLSVGHVDEFLSFVP
APDRKGFRLLLASPRSCYKLFQEQQNEGHGEALLFEGIKKKKQQKIKNILSNKTLREHNS
FVERCIDWNRELLKRELGLAESDIIDIPQLFKLKEFSKAEAFFPNMVNMLVLGKHLGIPK
PFGPVINGRCCLEEKVCSLLEPLGLQCTFINDFFTYHIRHGEVHCGTNVRRKPFSFKWWN
MVP
Target Bioclass
Enzyme
Family
WD repeat CDC20/Fizzy family
Subcellular location
Cytoplasm; Nucleus
Function
Substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. Acts as an adapter for APC/C to target the DNA-end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation. Through the regulation of RBBP8/CtIP protein turnover, may play a role in DNA damage response, favoring DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR).
Uniprot ID
Q9UM11
Ensemble ID
ENST00000313639.8
HGNC ID
HGNC:24824
ChEMBL ID
CHEMBL4523493

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C395(0.93); C403(0.93)  LDD1093  [1]
MPP-AC
 Probe Info  		N.A.  LDD0428  [2]
TER-AC
 Probe Info  		N.A.  LDD0426  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 PaTu 8988t C395(0.93); C403(0.93)  LDD1093  [1]
 LDCM0216  AC100 PaTu 8988t C395(1.20); C403(1.20)  LDD1095  [1]
 LDCM0217  AC101 PaTu 8988t C395(1.15); C403(1.15)  LDD1096  [1]
 LDCM0218  AC102 PaTu 8988t C395(1.02); C403(1.02)  LDD1097  [1]
 LDCM0219  AC103 PaTu 8988t C395(0.99); C403(0.99)  LDD1098  [1]
 LDCM0220  AC104 PaTu 8988t C395(0.84); C403(0.84)  LDD1099  [1]
 LDCM0221  AC105 PaTu 8988t C395(0.98); C403(0.98)  LDD1100  [1]
 LDCM0222  AC106 PaTu 8988t C395(1.14); C403(1.14)  LDD1101  [1]
 LDCM0223  AC107 PaTu 8988t C395(0.87); C403(0.87)  LDD1102  [1]
 LDCM0224  AC108 PaTu 8988t C395(1.15); C403(1.15)  LDD1103  [1]
 LDCM0225  AC109 PaTu 8988t C395(1.44); C403(1.44)  LDD1104  [1]
 LDCM0227  AC110 PaTu 8988t C395(1.34); C403(1.34)  LDD1106  [1]
 LDCM0228  AC111 PaTu 8988t C395(1.13); C403(1.13)  LDD1107  [1]
 LDCM0229  AC112 PaTu 8988t C395(1.02); C403(1.02)  LDD1108  [1]
 LDCM0263  AC143 PaTu 8988t C395(0.83); C403(0.83)  LDD1142  [1]
 LDCM0264  AC144 PaTu 8988t C395(0.92); C403(0.92)  LDD1143  [1]
 LDCM0265  AC145 PaTu 8988t C395(0.82); C403(0.82)  LDD1144  [1]
 LDCM0266  AC146 PaTu 8988t C395(1.23); C403(1.23)  LDD1145  [1]
 LDCM0267  AC147 PaTu 8988t C395(0.99); C403(0.99)  LDD1146  [1]
 LDCM0268  AC148 PaTu 8988t C395(0.83); C403(0.83)  LDD1147  [1]
 LDCM0269  AC149 PaTu 8988t C395(0.85); C403(0.85)  LDD1148  [1]
 LDCM0271  AC150 PaTu 8988t C395(1.07); C403(1.07)  LDD1150  [1]
 LDCM0272  AC151 PaTu 8988t C395(0.97); C403(0.97)  LDD1151  [1]
 LDCM0273  AC152 PaTu 8988t C395(0.88); C403(0.88)  LDD1152  [1]
 LDCM0274  AC153 PaTu 8988t C395(0.94); C403(0.94)  LDD1153  [1]
 LDCM0621  AC154 PaTu 8988t C395(0.98); C403(0.98)  LDD2166  [1]
 LDCM0622  AC155 PaTu 8988t C395(0.94); C403(0.94)  LDD2167  [1]
 LDCM0623  AC156 PaTu 8988t C395(0.89); C403(0.89)  LDD2168  [1]
 LDCM0624  AC157 PaTu 8988t C395(0.90); C403(0.90)  LDD2169  [1]
 LDCM0279  AC2 PaTu 8988t C395(1.22); C403(1.22)  LDD1158  [1]
 LDCM0290  AC3 PaTu 8988t C395(1.15); C403(1.15)  LDD1169  [1]
 LDCM0301  AC4 PaTu 8988t C395(0.94); C403(0.94)  LDD1180  [1]
 LDCM0312  AC5 PaTu 8988t C395(1.13); C403(1.13)  LDD1191  [1]
 LDCM0320  AC57 PaTu 8988t C395(1.02); C403(1.02)  LDD1199  [1]
 LDCM0321  AC58 PaTu 8988t C395(0.91); C403(0.91)  LDD1200  [1]
 LDCM0322  AC59 PaTu 8988t C395(1.00); C403(1.00)  LDD1201  [1]
 LDCM0324  AC60 PaTu 8988t C395(0.92); C403(0.92)  LDD1203  [1]
 LDCM0325  AC61 PaTu 8988t C395(1.06); C403(1.06)  LDD1204  [1]
 LDCM0326  AC62 PaTu 8988t C395(0.92); C403(0.92)  LDD1205  [1]
 LDCM0327  AC63 PaTu 8988t C395(1.06); C403(1.06)  LDD1206  [1]
 LDCM0328  AC64 PaTu 8988t C395(0.97); C403(0.97)  LDD1207  [1]
 LDCM0329  AC65 PaTu 8988t C395(1.09); C403(1.09)  LDD1208  [1]
 LDCM0330  AC66 PaTu 8988t C395(1.04); C403(1.04)  LDD1209  [1]
 LDCM0331  AC67 PaTu 8988t C395(0.96); C403(0.96)  LDD1210  [1]
 LDCM0332  AC68 PaTu 8988t C395(1.05); C403(1.05)  LDD1211  [1]
 LDCM0333  AC69 PaTu 8988t C395(0.83); C403(0.83)  LDD1212  [1]
 LDCM0335  AC70 PaTu 8988t C395(1.00); C403(1.00)  LDD1214  [1]
 LDCM0336  AC71 PaTu 8988t C395(1.12); C403(1.12)  LDD1215  [1]
 LDCM0337  AC72 PaTu 8988t C395(1.00); C403(1.00)  LDD1216  [1]
 LDCM0338  AC73 PaTu 8988t C395(1.05); C403(1.05)  LDD1217  [1]
 LDCM0339  AC74 PaTu 8988t C395(1.18); C403(1.18)  LDD1218  [1]
 LDCM0340  AC75 PaTu 8988t C395(1.12); C403(1.12)  LDD1219  [1]
 LDCM0341  AC76 PaTu 8988t C395(1.07); C403(1.07)  LDD1220  [1]
 LDCM0342  AC77 PaTu 8988t C395(1.06); C403(1.06)  LDD1221  [1]
 LDCM0343  AC78 PaTu 8988t C395(1.04); C403(1.04)  LDD1222  [1]
 LDCM0344  AC79 PaTu 8988t C395(1.08); C403(1.08)  LDD1223  [1]
 LDCM0346  AC80 PaTu 8988t C395(1.06); C403(1.06)  LDD1225  [1]
 LDCM0347  AC81 PaTu 8988t C395(1.09); C403(1.09)  LDD1226  [1]
 LDCM0348  AC82 PaTu 8988t C395(1.05); C403(1.05)  LDD1227  [1]
 LDCM0365  AC98 PaTu 8988t C395(1.30); C403(1.30)  LDD1244  [1]
 LDCM0366  AC99 PaTu 8988t C395(0.80); C403(0.80)  LDD1245  [1]
 LDCM0367  CL1 PaTu 8988t C395(0.83); C403(0.83)  LDD1246  [1]
 LDCM0368  CL10 PaTu 8988t C395(0.66); C403(0.66)  LDD1247  [1]
 LDCM0369  CL100 PaTu 8988t C395(0.98); C403(0.98)  LDD1248  [1]
 LDCM0379  CL11 PaTu 8988t C395(0.74); C403(0.74)  LDD1258  [1]
 LDCM0390  CL12 PaTu 8988t C395(0.97); C403(0.97)  LDD1269  [1]
 LDCM0396  CL125 PaTu 8988t C395(0.88); C403(0.88)  LDD1275  [1]
 LDCM0397  CL126 PaTu 8988t C395(0.64); C403(0.64)  LDD1276  [1]
 LDCM0398  CL127 PaTu 8988t C395(0.97); C403(0.97)  LDD1277  [1]
 LDCM0399  CL128 PaTu 8988t C395(1.08); C403(1.08)  LDD1278  [1]
 LDCM0400  CL13 PaTu 8988t C395(0.55); C403(0.55)  LDD1279  [1]
 LDCM0401  CL14 PaTu 8988t C395(0.50); C403(0.50)  LDD1280  [1]
 LDCM0402  CL15 PaTu 8988t C395(0.81); C403(0.81)  LDD1281  [1]
 LDCM0407  CL2 PaTu 8988t C395(0.82); C403(0.82)  LDD1286  [1]
 LDCM0418  CL3 PaTu 8988t C395(0.75); C403(0.75)  LDD1297  [1]
 LDCM0429  CL4 PaTu 8988t C395(0.85); C403(0.85)  LDD1308  [1]
 LDCM0440  CL5 PaTu 8988t C395(0.71); C403(0.71)  LDD1319  [1]
 LDCM0451  CL6 PaTu 8988t C395(0.76); C403(0.76)  LDD1330  [1]
 LDCM0453  CL61 PaTu 8988t C395(0.95); C403(0.95)  LDD1332  [1]
 LDCM0454  CL62 PaTu 8988t C395(1.01); C403(1.01)  LDD1333  [1]
 LDCM0455  CL63 PaTu 8988t C395(1.11); C403(1.11)  LDD1334  [1]
 LDCM0456  CL64 PaTu 8988t C395(0.84); C403(0.84)  LDD1335  [1]
 LDCM0457  CL65 PaTu 8988t C395(1.15); C403(1.15)  LDD1336  [1]
 LDCM0458  CL66 PaTu 8988t C395(0.84); C403(0.84)  LDD1337  [1]
 LDCM0459  CL67 PaTu 8988t C395(1.12); C403(1.12)  LDD1338  [1]
 LDCM0460  CL68 PaTu 8988t C395(0.90); C403(0.90)  LDD1339  [1]
 LDCM0461  CL69 PaTu 8988t C395(0.90); C403(0.90)  LDD1340  [1]
 LDCM0462  CL7 PaTu 8988t C395(0.80); C403(0.80)  LDD1341  [1]
 LDCM0463  CL70 PaTu 8988t C395(0.91); C403(0.91)  LDD1342  [1]
 LDCM0464  CL71 PaTu 8988t C395(0.96); C403(0.96)  LDD1343  [1]
 LDCM0465  CL72 PaTu 8988t C395(1.06); C403(1.06)  LDD1344  [1]
 LDCM0466  CL73 PaTu 8988t C395(0.91); C403(0.91)  LDD1345  [1]
 LDCM0467  CL74 PaTu 8988t C395(1.03); C403(1.03)  LDD1346  [1]
 LDCM0473  CL8 PaTu 8988t C395(1.02); C403(1.02)  LDD1352  [1]
 LDCM0484  CL9 PaTu 8988t C395(0.73); C403(0.73)  LDD1363  [1]
 LDCM0486  CL91 PaTu 8988t C395(1.08); C403(1.08)  LDD1365  [1]
 LDCM0487  CL92 PaTu 8988t C395(0.79); C403(0.79)  LDD1366  [1]
 LDCM0488  CL93 PaTu 8988t C395(0.92); C403(0.92)  LDD1367  [1]
 LDCM0489  CL94 PaTu 8988t C395(0.96); C403(0.96)  LDD1368  [1]
 LDCM0490  CL95 PaTu 8988t C395(0.91); C403(0.91)  LDD1369  [1]
 LDCM0491  CL96 PaTu 8988t C395(0.67); C403(0.67)  LDD1370  [1]
 LDCM0492  CL97 PaTu 8988t C395(0.92); C403(0.92)  LDD1371  [1]
 LDCM0493  CL98 PaTu 8988t C395(0.92); C403(0.92)  LDD1372  [1]
 LDCM0494  CL99 PaTu 8988t C395(1.11); C403(1.11)  LDD1373  [1]
 LDCM0468  Fragment33 PaTu 8988t C395(0.98); C403(0.98)  LDD1347  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cell division cycle protein 27 homolog (CDC27) APC3/CDC27 family P30260
Serine/threonine-protein kinase MAK (MAK) CMGC Ser/Thr protein kinase family P20794
Ribonucleoside-diphosphate reductase subunit M2 (RRM2) Ribonucleoside diphosphate reductase small chain family P31350
von Hippel-Lindau disease tumor suppressor (VHL) VHL family P40337
S-phase kinase-associated protein 2 (SKP2) . Q13309
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Claspin (CLSPN) Claspin family Q9HAW4
Cell cycle checkpoint protein RAD17 (RAD17) Rad17/RAD24 family O75943
Mitotic spindle assembly checkpoint protein MAD2B (MAD2L2) . Q9UI95

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Differently Tagged Probes for Protein Profiling of Mitochondria. Chembiochem. 2019 May 2;20(9):1155-1160. doi: 10.1002/cbic.201800735. Epub 2019 Mar 26.