Details of the Target

General Information of Target

Target ID LDTP13533
Target Name Long-chain-fatty-acid--CoA ligase 5 (ACSL5)
Gene Name ACSL5
Gene ID 51703
Synonyms
ACS5; FACL5; Long-chain-fatty-acid--CoA ligase 5; EC 6.2.1.3; Arachidonate--CoA ligase; EC 6.2.1.15; Long-chain acyl-CoA synthetase 5; LACS 5
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MFKKFDEKENVSNCIQLKTSVIKGIKNQLIEQFPGIEPWLNQIMPKKDPVKIVRCHEHIE
ILTVNGELLFFRQREGPFYPTLRLLHKYPFILPHQQVDKGAIKFVLSGANIMCPGLTSPG
AKLYPAAVDTIVAIMAEGKQHALCVGVMKMSAEDIEKVNKGIGIENIHYLNDGLWHMKTY
K
Target Bioclass
Enzyme
Family
ATP-dependent AMP-binding enzyme family
Subcellular location
Mitochondrion
Function
Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage. Utilizes a wide range of saturated fatty acids with a preference for C16-C18 unsaturated fatty acids. It was suggested that it may also stimulate fatty acid oxidation. At the villus tip of the crypt-villus axis of the small intestine may sensitize epithelial cells to apoptosis specifically triggered by the death ligand TRAIL. May have a role in the survival of glioma cells.
Uniprot ID
Q9ULC5
Ensemble ID
ENST00000354655.9
HGNC ID
HGNC:16526
ChEMBL ID
CHEMBL4105818

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HT115 SNV: p.I273T DBIA    Probe Info 
MCC26 SNV: p.V439M; p.E559Q .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
YN-1
 Probe Info  		100.00  LDD0444  [1]
STPyne
 Probe Info  		K344(4.93); K361(1.09); K546(0.38); K628(10.00)  LDD0277  [2]
ONAyne
 Probe Info  		K361(3.48)  LDD0275  [2]
IPM
 Probe Info  		C69(0.00); C121(0.00); C70(0.00); C322(0.00)  LDD0241  [3]
DBIA
 Probe Info  		C177(2.54); C93(2.75); C666(1.87); C378(2.64)  LDD3311  [4]
BTD
 Probe Info  		C121(1.15)  LDD2090  [5]
Alkyne-RA190
 Probe Info  		2.07  LDD0299  [6]
IA-alkyne
 Probe Info  		C322(1.02)  LDD0346  [7]
IPIAA_H
 Probe Info  		N.A.  LDD0030  [8]
NAIA_4
 Probe Info  		C259(0.00); C288(0.00); C322(0.00)  LDD2226  [9]
W1
 Probe Info  		C610(0.00); C93(0.00); C69(0.00); C70(0.00)  LDD0236  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C121(0.51); C322(0.47)  LDD2142  [5]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C121(1.33)  LDD2117  [5]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C121(9.53); C322(4.80); C93(1.66)  LDD1702  [5]
 LDCM0625  F8 Ramos C322(3.45); C185(1.34); 1.44; C610(0.88)  LDD2187  [7]
 LDCM0572  Fragment10 Ramos C322(0.43); C185(0.28); 3.62; C610(0.52)  LDD2189  [7]
 LDCM0573  Fragment11 Ramos C121(0.85); C322(0.72); C185(0.31); 9.76  LDD2190  [7]
 LDCM0574  Fragment12 Ramos C322(0.89); C610(0.59); C93(1.38)  LDD2191  [7]
 LDCM0575  Fragment13 Ramos C322(0.47); 1.12; C610(0.91); C93(0.98)  LDD2192  [7]
 LDCM0576  Fragment14 Ramos C121(1.04); C322(1.35); C185(0.46); 1.37  LDD2193  [7]
 LDCM0579  Fragment20 Ramos C610(0.61)  LDD2194  [7]
 LDCM0580  Fragment21 Ramos 0.99; C610(0.60); C93(1.01)  LDD2195  [7]
 LDCM0582  Fragment23 Ramos C322(0.57); 0.61; C610(0.87); C93(0.76)  LDD2196  [7]
 LDCM0578  Fragment27 Ramos C185(0.78); 0.68; C610(0.87); C93(0.84)  LDD2197  [7]
 LDCM0586  Fragment28 Ramos C121(2.41); C322(0.91); 0.90; C610(0.56)  LDD2198  [7]
 LDCM0588  Fragment30 Ramos C322(0.62); C185(0.31); 0.94; C610(0.80)  LDD2199  [7]
 LDCM0589  Fragment31 Ramos 1.07; C610(0.92); C93(0.89)  LDD2200  [7]
 LDCM0590  Fragment32 Ramos C322(0.60); C185(0.23); C610(0.51); C93(1.59)  LDD2201  [7]
 LDCM0468  Fragment33 Ramos C322(0.56); 1.06; C610(0.72); C93(0.96)  LDD2202  [7]
 LDCM0596  Fragment38 Ramos C322(0.36); 0.68; C610(0.51); C93(0.90)  LDD2203  [7]
 LDCM0566  Fragment4 Ramos C322(1.63); C185(0.99); C610(0.83); C93(2.74)  LDD2184  [7]
 LDCM0610  Fragment52 Ramos C322(0.32); C93(1.24)  LDD2204  [7]
 LDCM0614  Fragment56 Ramos C322(0.50); C185(0.32); 1.57; C610(0.66)  LDD2205  [7]
 LDCM0569  Fragment7 Ramos C322(1.42); C185(1.02); 9.60; C610(0.66)  LDD2186  [7]
 LDCM0571  Fragment9 Ramos C185(0.25); C610(0.72); C93(2.47)  LDD2188  [7]
 LDCM0015  HNE MDA-MB-231 C322(1.02)  LDD0346  [7]
 LDCM0022  KB02 Ramos C322(1.38); C185(2.29); C610(0.47); C93(1.44)  LDD2182  [7]
 LDCM0023  KB03 MDA-MB-231 C121(9.77); C93(4.46); C185(1.13)  LDD1701  [5]
 LDCM0024  KB05 G361 C177(2.54); C93(2.75); C666(1.87); C378(2.64)  LDD3311  [4]
 LDCM0509  N-(4-bromo-3,5-dimethylphenyl)-2-nitroacetamide MDA-MB-231 C121(1.09)  LDD2102  [5]
 LDCM0497  Nucleophilic fragment 11b MDA-MB-231 C121(1.15)  LDD2090  [5]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C121(0.98)  LDD2092  [5]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C121(1.12)  LDD2094  [5]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C121(1.05)  LDD2098  [5]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C121(1.09)  LDD2099  [5]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C121(0.97)  LDD2100  [5]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C121(0.68)  LDD2104  [5]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C121(1.43)  LDD2105  [5]
 LDCM0513  Nucleophilic fragment 19b MDA-MB-231 C121(0.56)  LDD2106  [5]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C121(0.97)  LDD2107  [5]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C121(1.48)  LDD2109  [5]
 LDCM0517  Nucleophilic fragment 21b MDA-MB-231 C121(0.90)  LDD2110  [5]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C121(1.67)  LDD2119  [5]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C121(1.20)  LDD2123  [5]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C121(1.10)  LDD2129  [5]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C322(1.69)  LDD2134  [5]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C121(1.12)  LDD2135  [5]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C121(0.88)  LDD2140  [5]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C121(0.87)  LDD2141  [5]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C121(1.71)  LDD2144  [5]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C121(1.03)  LDD2145  [5]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C121(1.32)  LDD2146  [5]
 LDCM0559  Nucleophilic fragment 9b MDA-MB-231 C121(2.10)  LDD2153  [5]
 LDCM0131  RA190 MM1.R 2.07  LDD0299  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 7 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Solute carrier family 7 member 14 (SLC7A14) Cationic amino acid transporter (CAT) (TC 2.A.3.3) family Q8TBB6
Hepatic sodium/bile acid cotransporter (SLC10A1) Bile acid:sodium symporter (BASS) family Q14973
Sodium-dependent organic anion transporter (SLC10A6) Bile acid:sodium symporter (BASS) family Q3KNW5
Monocarboxylate transporter 2 (SLC16A7) Monocarboxylate porter (TC 2.A.1.13) family O60669
Aquaporin-6 (AQP6) MIP/aquaporin (TC 1.A.8) family Q13520
Peroxisome assembly protein 12 (PEX12) Pex2/pex10/pex12 family O00623
Transmembrane protein 14B (TMEM14B) TMEM14 family Q9NUH8
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Reticulophagy regulator 3 (RETREG3) RETREG family Q86VR2
Receptor-binding cancer antigen expressed on SiSo cells (EBAG9) . O00559

References

1 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
6 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.
7 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
8 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
9 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264