Details of the Target

General Information of Target

Target ID LDTP13390
Target Name Kelch-like protein 21 (KLHL21)
Gene Name KLHL21
Gene ID 9903
Synonyms
KIAA0469; Kelch-like protein 21
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MLPRLICINDYEQHAKSVLPKSIYDYYRSGANDEETLADNIAAFSRWKLYPRMLRNVAET
DLSTSVLGQRVSMPICVGATAMQRMAHVDGELATVRACQSLGTGMMLSSWATSSIEEVAE
AGPEALRWLQLYIYKDREVTKKLVRQAEKMGYKAIFVTVDTPYLGNRLDDVRNRFKLPPQ
LRMKNFETSTLSFSPEENFGDDSGLAAYVAKAIDPSISWEDIKWLRRLTSLPIVAKGILR
GDDAREAVKHGLNGILVSNHGARQLDGVPATIDVLPEIVEAVEGKVEVFLDGGVRKGTDV
LKALALGAKAVFVGRPIVWGLAFQGEKGVQDVLEILKEEFRLAMALSGCQNVKVIDKTLV
RKNPLAVSKI
Target Bioclass
Other
Subcellular location
Cytoplasm, cytoskeleton, spindle
Function
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for efficient chromosome alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
Uniprot ID
Q9UJP4
Ensemble ID
ENST00000377658.8
HGNC ID
HGNC:29041

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A498 SNV: p.D452Y .
HDMYZ SNV: p.W205G DBIA    Probe Info 
HUPT3 SNV: p.R275L .
MCC13 SNV: p.E286K .
MCC26 Substitution: p.P457S .
PC9 SNV: p.A285S .
RKO Deletion: p.K513SfsTer18 DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Acrolein
 Probe Info  		N.A.  LDD0222  [1]
DBIA
 Probe Info  		C351(0.91)  LDD0078  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0246  AC128 HCT 116 C351(0.81)  LDD0563  [2]
 LDCM0247  AC129 HCT 116 C351(0.95)  LDD0564  [2]
 LDCM0249  AC130 HCT 116 C351(0.78)  LDD0566  [2]
 LDCM0250  AC131 HCT 116 C351(0.95)  LDD0567  [2]
 LDCM0251  AC132 HCT 116 C351(0.91)  LDD0568  [2]
 LDCM0252  AC133 HCT 116 C351(0.76)  LDD0569  [2]
 LDCM0253  AC134 HCT 116 C351(0.95)  LDD0570  [2]
 LDCM0254  AC135 HCT 116 C351(0.89)  LDD0571  [2]
 LDCM0255  AC136 HCT 116 C351(0.96)  LDD0572  [2]
 LDCM0256  AC137 HCT 116 C351(1.00)  LDD0573  [2]
 LDCM0257  AC138 HCT 116 C351(0.89)  LDD0574  [2]
 LDCM0258  AC139 HCT 116 C351(1.00)  LDD0575  [2]
 LDCM0260  AC140 HCT 116 C351(0.84)  LDD0577  [2]
 LDCM0261  AC141 HCT 116 C351(0.95)  LDD0578  [2]
 LDCM0262  AC142 HCT 116 C351(1.43)  LDD0579  [2]
 LDCM0263  AC143 HCT 116 C351(1.10)  LDD0580  [2]
 LDCM0264  AC144 HCT 116 C351(0.78)  LDD0581  [2]
 LDCM0265  AC145 HCT 116 C351(0.87)  LDD0582  [2]
 LDCM0266  AC146 HCT 116 C351(0.93)  LDD0583  [2]
 LDCM0267  AC147 HCT 116 C351(0.86)  LDD0584  [2]
 LDCM0268  AC148 HCT 116 C351(0.91)  LDD0585  [2]
 LDCM0269  AC149 HCT 116 C351(0.94)  LDD0586  [2]
 LDCM0271  AC150 HCT 116 C351(0.97)  LDD0588  [2]
 LDCM0272  AC151 HCT 116 C351(1.05)  LDD0589  [2]
 LDCM0273  AC152 HCT 116 C351(0.86)  LDD0590  [2]
 LDCM0274  AC153 HCT 116 C351(1.05)  LDD0591  [2]
 LDCM0621  AC154 HCT 116 C351(0.86)  LDD2158  [2]
 LDCM0622  AC155 HCT 116 C351(0.92)  LDD2159  [2]
 LDCM0623  AC156 HCT 116 C351(0.97)  LDD2160  [2]
 LDCM0624  AC157 HCT 116 C351(0.91)  LDD2161  [2]
 LDCM0332  AC68 HCT 116 C351(0.99)  LDD0649  [2]
 LDCM0333  AC69 HCT 116 C351(0.96)  LDD0650  [2]
 LDCM0335  AC70 HCT 116 C351(0.87)  LDD0652  [2]
 LDCM0336  AC71 HCT 116 C351(1.08)  LDD0653  [2]
 LDCM0337  AC72 HCT 116 C351(0.87)  LDD0654  [2]
 LDCM0338  AC73 HCT 116 C351(0.96)  LDD0655  [2]
 LDCM0339  AC74 HCT 116 C351(1.00)  LDD0656  [2]
 LDCM0340  AC75 HCT 116 C351(1.07)  LDD0657  [2]
 LDCM0341  AC76 HCT 116 C351(0.85)  LDD0658  [2]
 LDCM0342  AC77 HCT 116 C351(0.89)  LDD0659  [2]
 LDCM0343  AC78 HCT 116 C351(1.05)  LDD0660  [2]
 LDCM0344  AC79 HCT 116 C351(0.92)  LDD0661  [2]
 LDCM0346  AC80 HCT 116 C351(0.82)  LDD0663  [2]
 LDCM0347  AC81 HCT 116 C351(0.96)  LDD0664  [2]
 LDCM0348  AC82 HCT 116 C351(1.28)  LDD0665  [2]
 LDCM0349  AC83 HCT 116 C351(0.88)  LDD0666  [2]
 LDCM0350  AC84 HCT 116 C351(0.88)  LDD0667  [2]
 LDCM0351  AC85 HCT 116 C351(1.04)  LDD0668  [2]
 LDCM0352  AC86 HCT 116 C351(0.93)  LDD0669  [2]
 LDCM0353  AC87 HCT 116 C351(0.86)  LDD0670  [2]
 LDCM0354  AC88 HCT 116 C351(0.86)  LDD0671  [2]
 LDCM0355  AC89 HCT 116 C351(0.92)  LDD0672  [2]
 LDCM0357  AC90 HCT 116 C351(0.93)  LDD0674  [2]
 LDCM0358  AC91 HCT 116 C351(0.82)  LDD0675  [2]
 LDCM0359  AC92 HCT 116 C351(0.79)  LDD0676  [2]
 LDCM0360  AC93 HCT 116 C351(0.83)  LDD0677  [2]
 LDCM0361  AC94 HCT 116 C351(0.97)  LDD0678  [2]
 LDCM0362  AC95 HCT 116 C351(1.13)  LDD0679  [2]
 LDCM0363  AC96 HCT 116 C351(1.00)  LDD0680  [2]
 LDCM0364  AC97 HCT 116 C351(0.77)  LDD0681  [2]
 LDCM0020  ARS-1620 HCC44 C351(0.91)  LDD0078  [2]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [1]
 LDCM0367  CL1 HCT 116 C351(0.84)  LDD0684  [2]
 LDCM0368  CL10 HCT 116 C351(0.88)  LDD0685  [2]
 LDCM0379  CL11 HCT 116 C351(1.07)  LDD0696  [2]
 LDCM0390  CL12 HCT 116 C351(0.89)  LDD0707  [2]
 LDCM0400  CL13 HCT 116 C351(0.99)  LDD0717  [2]
 LDCM0401  CL14 HCT 116 C351(0.78)  LDD0718  [2]
 LDCM0402  CL15 HCT 116 C351(1.25)  LDD0719  [2]
 LDCM0407  CL2 HCT 116 C351(0.90)  LDD0724  [2]
 LDCM0418  CL3 HCT 116 C351(1.35)  LDD0735  [2]
 LDCM0429  CL4 HCT 116 C351(0.81)  LDD0746  [2]
 LDCM0440  CL5 HCT 116 C351(1.04)  LDD0757  [2]
 LDCM0451  CL6 HCT 116 C351(1.28)  LDD0768  [2]
 LDCM0462  CL7 HCT 116 C351(1.02)  LDD0779  [2]
 LDCM0469  CL76 HCT 116 C351(0.99)  LDD0786  [2]
 LDCM0470  CL77 HCT 116 C351(0.84)  LDD0787  [2]
 LDCM0471  CL78 HCT 116 C351(1.06)  LDD0788  [2]
 LDCM0472  CL79 HCT 116 C351(0.83)  LDD0789  [2]
 LDCM0473  CL8 HCT 116 C351(1.05)  LDD0790  [2]
 LDCM0474  CL80 HCT 116 C351(1.11)  LDD0791  [2]
 LDCM0475  CL81 HCT 116 C351(1.11)  LDD0792  [2]
 LDCM0476  CL82 HCT 116 C351(0.93)  LDD0793  [2]
 LDCM0477  CL83 HCT 116 C351(0.80)  LDD0794  [2]
 LDCM0478  CL84 HCT 116 C351(1.06)  LDD0795  [2]
 LDCM0479  CL85 HCT 116 C351(0.97)  LDD0796  [2]
 LDCM0480  CL86 HCT 116 C351(0.86)  LDD0797  [2]
 LDCM0481  CL87 HCT 116 C351(0.87)  LDD0798  [2]
 LDCM0482  CL88 HCT 116 C351(0.91)  LDD0799  [2]
 LDCM0483  CL89 HCT 116 C351(1.10)  LDD0800  [2]
 LDCM0484  CL9 HCT 116 C351(0.68)  LDD0801  [2]
 LDCM0485  CL90 HCT 116 C351(0.79)  LDD0802  [2]
 LDCM0022  KB02 769-P C351(1.34)  LDD2246  [3]
 LDCM0023  KB03 42-MG-BA C351(1.71)  LDD2661  [3]
 LDCM0024  KB05 MKN-1 C351(1.45)  LDD3331  [3]

References

1 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840