Details of the Target

General Information of Target

Target ID LDTP12662
Target Name Protein-L-isoaspartate O-methyltransferase domain-containing protein 2 (PCMTD2)
Gene Name PCMTD2
Gene ID 55251
Synonyms
C20orf36; Protein-L-isoaspartate O-methyltransferase domain-containing protein 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTAIKHALQRDIFTPNDERLLSIVNVCKAGKKKKNCFLCATVTTERPVQVKVVKVKKSDK
GDFYKRQIAWALRDLAVVDAKDAIKENPEFDLHFEKIYKWVASSTAEKNAFISCIWKLNQ
RYLRKKIDFVNVSSQLLEESVPSGENQSVTGGDEEVVDEYQELNAREEQDIEIMMEGCEY
AISNAEAFAEKLSRELQVLDGANIQSIMASEKQVNILMKLLDEALKEVDQIELKLSSYEE
MLQSVKEQMDQISESNHLIHLSNTNNVKLLSEIEFLVNHMDLAKGHIKALQEGDLASSRG
IEACTNAADALLQCMNVALRPGHDLLLAVKQQQQRFSDLRELFARRLASHLNNVFVQQGH
DQSSTLAQHSVELTLPNHHPFHRDLLRYAKLMEWLKSTDYGKYEGLTKNYMDYLSRLYER
EIKDFFEVAKIKMTGTTKESKKFATLPRKESAVKQETESLHGSSGKLTGSTSSLNKLSVQ
SSGNRRSQSSSLLDMGNMSASDLDVADRTKFDKIFEQVLSELEPLCLAEQDFISKFFKLQ
QHQSMPGTMAEAEDLDGGTLSRQHNCGTPLPVSSEKDMIRQMMIKIFRCIEPELNNLIAL
GDKIDSFNSLYMLVKMSHHVWTAQNVDPASFLSTTLGNVLVTVKRNFDKCISNQIRQMEE
VKISKKSKVGILPFVAEFEEFAGLAESIFKNAERRGDLDKAYTKLIRGVFVNVEKVANES
QKTPRDVVMMENFHHIFATLSRLKISCLEAEKKEAKQKYTDHLQSYVIYSLGQPLEKLNH
FFEGVEARVAQGIREEEVSYQLAFNKQELRKVIKEYPGKEVKKGLDNLYKKVDKHLCEEE
NLLQVVWHSMQDEFIRQYKHFEGLIARCYPGSGVTMEFTIQDILDYCSSIAQSH
Target Bioclass
Enzyme
Family
Methyltransferase superfamily, L-isoaspartyl/D-aspartyl protein methyltransferase family
Subcellular location
Cytoplasm
Function
May act as a substrate recognition component of an ECS (Elongin BC-CUL5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. May bind to the methyltransferase cofactor S-adenosylmethionine (AdoMet) via the N-terminal AdoMet binding motif, but probably does not display methyltransferase activity.
Uniprot ID
Q9NV79
Ensemble ID
ENST00000308824.11
HGNC ID
HGNC:15882

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
PC14 SNV: p.D37Y .
TE1 SNV: p.T196A .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C165(4.52)  LDD3360  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [2]
NAIA_4
 Probe Info  		C223(0.00); C311(0.00)  LDD2226  [3]
AOyne
 Probe Info  		15.00  LDD0443  [4]
NAIA_5
 Probe Info  		N.A.  LDD2223  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0263  AC143 PaTu 8988t C141(0.82); C150(0.82); C157(0.82)  LDD1142  [5]
 LDCM0264  AC144 PaTu 8988t C141(0.85); C150(0.85); C157(0.85)  LDD1143  [5]
 LDCM0265  AC145 PaTu 8988t C141(1.14); C150(1.14); C157(1.14)  LDD1144  [5]
 LDCM0266  AC146 PaTu 8988t C141(0.62); C150(0.62); C157(0.62)  LDD1145  [5]
 LDCM0267  AC147 PaTu 8988t C141(0.98); C150(0.98); C157(0.98)  LDD1146  [5]
 LDCM0268  AC148 PaTu 8988t C141(0.77); C150(0.77); C157(0.77)  LDD1147  [5]
 LDCM0269  AC149 PaTu 8988t C141(0.79); C150(0.79); C157(0.79)  LDD1148  [5]
 LDCM0271  AC150 PaTu 8988t C141(0.89); C150(0.89); C157(0.89)  LDD1150  [5]
 LDCM0272  AC151 PaTu 8988t C141(0.95); C150(0.95); C157(0.95)  LDD1151  [5]
 LDCM0273  AC152 PaTu 8988t C141(1.12); C150(1.12); C157(1.12)  LDD1152  [5]
 LDCM0274  AC153 PaTu 8988t C141(0.87); C150(0.87); C157(0.87)  LDD1153  [5]
 LDCM0621  AC154 PaTu 8988t C141(0.79); C150(0.79); C157(0.79)  LDD2166  [5]
 LDCM0622  AC155 PaTu 8988t C141(0.76); C150(0.76); C157(0.76)  LDD2167  [5]
 LDCM0623  AC156 PaTu 8988t C141(0.71); C150(0.71); C157(0.71)  LDD2168  [5]
 LDCM0624  AC157 PaTu 8988t C141(1.10); C150(1.10); C157(1.10)  LDD2169  [5]
 LDCM0367  CL1 PaTu 8988t C141(1.34); C150(1.34); C157(1.34)  LDD1246  [5]
 LDCM0368  CL10 PaTu 8988t C141(0.94); C150(0.94); C157(0.94)  LDD1247  [5]
 LDCM0379  CL11 PaTu 8988t C141(1.19); C150(1.19); C157(1.19)  LDD1258  [5]
 LDCM0390  CL12 PaTu 8988t C141(1.08); C150(1.08); C157(1.08)  LDD1269  [5]
 LDCM0400  CL13 PaTu 8988t C141(0.88); C150(0.88); C157(0.88)  LDD1279  [5]
 LDCM0401  CL14 PaTu 8988t C141(1.20); C150(1.20); C157(1.20)  LDD1280  [5]
 LDCM0402  CL15 PaTu 8988t C141(1.20); C150(1.20); C157(1.20)  LDD1281  [5]
 LDCM0403  CL16 PaTu 8988t C141(1.26); C150(1.26); C157(1.26)  LDD1282  [5]
 LDCM0404  CL17 PaTu 8988t C141(1.37); C150(1.37); C157(1.37)  LDD1283  [5]
 LDCM0405  CL18 PaTu 8988t C141(1.16); C150(1.16); C157(1.16)  LDD1284  [5]
 LDCM0406  CL19 PaTu 8988t C141(1.22); C150(1.22); C157(1.22)  LDD1285  [5]
 LDCM0407  CL2 PaTu 8988t C141(1.14); C150(1.14); C157(1.14)  LDD1286  [5]
 LDCM0408  CL20 PaTu 8988t C141(0.97); C150(0.97); C157(0.97)  LDD1287  [5]
 LDCM0409  CL21 PaTu 8988t C141(1.37); C150(1.37); C157(1.37)  LDD1288  [5]
 LDCM0410  CL22 PaTu 8988t C141(1.46); C150(1.46); C157(1.46)  LDD1289  [5]
 LDCM0411  CL23 PaTu 8988t C141(1.27); C150(1.27); C157(1.27)  LDD1290  [5]
 LDCM0412  CL24 PaTu 8988t C141(1.59); C150(1.59); C157(1.59)  LDD1291  [5]
 LDCM0413  CL25 PaTu 8988t C141(1.08); C150(1.08); C157(1.08)  LDD1292  [5]
 LDCM0414  CL26 PaTu 8988t C141(0.89); C150(0.89); C157(0.89)  LDD1293  [5]
 LDCM0415  CL27 PaTu 8988t C141(1.23); C150(1.23); C157(1.23)  LDD1294  [5]
 LDCM0416  CL28 PaTu 8988t C141(0.93); C150(0.93); C157(0.93)  LDD1295  [5]
 LDCM0417  CL29 PaTu 8988t C141(1.21); C150(1.21); C157(1.21)  LDD1296  [5]
 LDCM0418  CL3 PaTu 8988t C141(1.67); C150(1.67); C157(1.67)  LDD1297  [5]
 LDCM0419  CL30 PaTu 8988t C141(1.10); C150(1.10); C157(1.10)  LDD1298  [5]
 LDCM0420  CL31 PaTu 8988t C141(0.96); C150(0.96); C157(0.96)  LDD1299  [5]
 LDCM0421  CL32 PaTu 8988t C141(0.92); C150(0.92); C157(0.92)  LDD1300  [5]
 LDCM0422  CL33 PaTu 8988t C141(0.83); C150(0.83); C157(0.83)  LDD1301  [5]
 LDCM0423  CL34 PaTu 8988t C141(1.07); C150(1.07); C157(1.07)  LDD1302  [5]
 LDCM0424  CL35 PaTu 8988t C141(0.92); C150(0.92); C157(0.92)  LDD1303  [5]
 LDCM0425  CL36 PaTu 8988t C141(0.98); C150(0.98); C157(0.98)  LDD1304  [5]
 LDCM0426  CL37 PaTu 8988t C141(0.78); C150(0.78); C157(0.78)  LDD1305  [5]
 LDCM0428  CL39 PaTu 8988t C141(1.12); C150(1.12); C157(1.12)  LDD1307  [5]
 LDCM0429  CL4 PaTu 8988t C141(0.83); C150(0.83); C157(0.83)  LDD1308  [5]
 LDCM0430  CL40 PaTu 8988t C141(1.00); C150(1.00); C157(1.00)  LDD1309  [5]
 LDCM0431  CL41 PaTu 8988t C141(1.07); C150(1.07); C157(1.07)  LDD1310  [5]
 LDCM0432  CL42 PaTu 8988t C141(0.94); C150(0.94); C157(0.94)  LDD1311  [5]
 LDCM0433  CL43 PaTu 8988t C141(0.91); C150(0.91); C157(0.91)  LDD1312  [5]
 LDCM0434  CL44 PaTu 8988t C141(0.93); C150(0.93); C157(0.93)  LDD1313  [5]
 LDCM0435  CL45 PaTu 8988t C141(0.76); C150(0.76); C157(0.76)  LDD1314  [5]
 LDCM0436  CL46 PaTu 8988t C141(1.35); C150(1.35); C157(1.35)  LDD1315  [5]
 LDCM0437  CL47 PaTu 8988t C141(1.18); C150(1.18); C157(1.18)  LDD1316  [5]
 LDCM0438  CL48 PaTu 8988t C141(1.29); C150(1.29); C157(1.29)  LDD1317  [5]
 LDCM0439  CL49 PaTu 8988t C141(1.42); C150(1.42); C157(1.42)  LDD1318  [5]
 LDCM0440  CL5 PaTu 8988t C141(0.98); C150(0.98); C157(0.98)  LDD1319  [5]
 LDCM0441  CL50 PaTu 8988t C141(1.30); C150(1.30); C157(1.30)  LDD1320  [5]
 LDCM0442  CL51 PaTu 8988t C141(1.99); C150(1.99); C157(1.99)  LDD1321  [5]
 LDCM0443  CL52 PaTu 8988t C141(1.39); C150(1.39); C157(1.39)  LDD1322  [5]
 LDCM0444  CL53 PaTu 8988t C141(1.93); C150(1.93); C157(1.93)  LDD1323  [5]
 LDCM0445  CL54 PaTu 8988t C141(1.68); C150(1.68); C157(1.68)  LDD1324  [5]
 LDCM0446  CL55 PaTu 8988t C141(1.72); C150(1.72); C157(1.72)  LDD1325  [5]
 LDCM0447  CL56 PaTu 8988t C141(0.40); C150(0.40); C157(0.40)  LDD1326  [5]
 LDCM0448  CL57 PaTu 8988t C141(1.39); C150(1.39); C157(1.39)  LDD1327  [5]
 LDCM0449  CL58 PaTu 8988t C141(1.63); C150(1.63); C157(1.63)  LDD1328  [5]
 LDCM0450  CL59 PaTu 8988t C141(1.53); C150(1.53); C157(1.53)  LDD1329  [5]
 LDCM0451  CL6 PaTu 8988t C141(0.86); C150(0.86); C157(0.86)  LDD1330  [5]
 LDCM0452  CL60 PaTu 8988t C141(1.52); C150(1.52); C157(1.52)  LDD1331  [5]
 LDCM0462  CL7 PaTu 8988t C141(1.26); C150(1.26); C157(1.26)  LDD1341  [5]
 LDCM0473  CL8 PaTu 8988t C141(1.56); C150(1.56); C157(1.56)  LDD1352  [5]
 LDCM0484  CL9 PaTu 8988t C141(0.92); C150(0.92); C157(0.92)  LDD1363  [5]
 LDCM0427  Fragment51 PaTu 8988t C141(1.32); C150(1.32); C157(1.32)  LDD1306  [5]
 LDCM0022  KB02 AU565 C223(1.53)  LDD2265  [1]
 LDCM0023  KB03 CAL-148 C223(1.53)  LDD2702  [1]
 LDCM0024  KB05 NCI-H3122 C165(4.52)  LDD3360  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Nitric oxide synthase 3 (NOS3) NOS family P29474
Serine protease HTRA2, mitochondrial (HTRA2) Peptidase S1C family O43464
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Junctophilin-3 (JPH3) Junctophilin family Q8WXH2
Alpha-synuclein (SNCA) Synuclein family P37840
Other
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ataxin-1 (ATXN1) ATXN1 family P54253
RING finger protein 11 (RNF11) . Q9Y3C5
Sprouty-related, EVH1 domain-containing protein 1 (SPRED1) . Q7Z699
TAR DNA-binding protein 43 (TARDBP) . Q13148

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
5 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.