Details of the Target

General Information of Target

Target ID LDTP12116
Target Name Uridine-cytidine kinase 1 (UCK1)
Gene Name UCK1
Gene ID 83549
Synonyms
URK1; Uridine-cytidine kinase 1; UCK 1; EC 2.7.1.48; Cytidine monophosphokinase 1; Uridine monophosphokinase 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MILLQHAVLPPPKQPSPSPPMSVATRSTGTLQLPPQKPFGQEASLPLAGEEELSKGGEQD
CALEELCKPLYCKLCNVTLNSAQQAQAHYQGKNHGKKLRNYYAANSCPPPARMSNVVEPA
ATPVVPVPPQMGSFKPGGRVILATENDYCKLCDASFSSPAVAQAHYQGKNHAKRLRLAEA
QSNSFSESSELGQRRARKEGNEFKMMPNRRNMYTVQNNSAGPYFNPRSRQRIPRDLAMCV
TPSGQFYCSMCNVGAGEEMEFRQHLESKQHKSKVSEQRYRNEMENLGYV
Target Bioclass
Enzyme
Family
Uridine kinase family
Function
Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Can use ATP or GTP as a phosphate donor. Can also phosphorylate cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine.
Uniprot ID
Q9HA47
Ensemble ID
ENST00000372208.7
HGNC ID
HGNC:14859
ChEMBL ID
CHEMBL5682

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
DOTC24510 SNV: p.L96F .
EGI1 SNV: p.M151T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
TH214
 Probe Info  		Y205(20.00)  LDD0258  [2]
DBIA
 Probe Info  		C241(1.18)  LDD3344  [3]
EA-probe
 Probe Info  		N.A.  LDD0440  [4]
IPM
 Probe Info  		C199(1.05)  LDD1701  [5]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [6]
IA-alkyne
 Probe Info  		C199(0.00); C236(0.00)  LDD0036  [6]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [7]
Lodoacetamide azide
 Probe Info  		C199(0.00); C236(0.00)  LDD0037  [6]
AOyne
 Probe Info  		15.00  LDD0443  [8]
NAIA_5
 Probe Info  		C199(0.00); C236(0.00)  LDD2223  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0026  4SU-RNA+native RNA HEK-293T C236(2.35)  LDD0372  [10]
 LDCM0156  Aniline NCI-H1299 12.87  LDD0403  [1]
 LDCM0175  Ethacrynic acid HeLa N.A.  LDD0440  [4]
 LDCM0022  KB02 IGR-37 C45(2.43)  LDD2396  [3]
 LDCM0023  KB03 MDA-MB-231 C199(1.05)  LDD1701  [5]
 LDCM0024  KB05 NCI-H146 C241(1.18)  LDD3344  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Fibroblast growth factor receptor 3 (FGFR3) Tyr protein kinase family P22607
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Gelsolin (GSN) Villin/gelsolin family P06396

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Chemoproteomic Profiling Reveals Ethacrynic Acid Targets Adenine Nucleotide Translocases to Impair Mitochondrial Function. Mol Pharm. 2018 Jun 4;15(6):2413-2422. doi: 10.1021/acs.molpharmaceut.8b00250. Epub 2018 May 15.
5 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
6 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
7 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
8 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
9 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
10 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625