Details of the Target

General Information of Target

Target ID LDTP12104
Target Name Putative E3 ubiquitin-protein ligase UNKL (UNKL)
Gene Name UNKL
Gene ID 64718
Synonyms
C16orf28; ZC3H5L; ZC3HDC5L; Putative E3 ubiquitin-protein ligase UNKL; EC 2.3.2.-; RING finger protein unkempt-like; Zinc finger CCCH domain-containing protein 5-like
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSRVVSLLLGAALLCGHGAFCRRVVSGQKVCFADFKHPCYKMAYFHELSSRVSFQEARLA
CESEGGVLLSLENEAEQKLIESMLQNLTKPGTGISDGDFWIGLWRNGDGQTSGACPDLYQ
WSDGSNSQYRNWYTDEPSCGSEKCVVMYHQPTANPGLGGPYLYQWNDDRCNMKHNYICKY
EPEINPTAPVEKPYLTNQPGDTHQNVVVTEAGIIPNLIYVVIPTIPLLLLILVAFGTCCF
QMLHKSKGRTKTSPNQSTLWISKSTRKESGMEV
Target Bioclass
Enzyme
Family
Unkempt family
Subcellular location
Cytoplasm
Function
May participate in a protein complex showing an E3 ligase activity regulated by RAC1. Ubiquitination is directed towards itself and possibly other substrates, such as SMARCD2/BAF60b. Intrinsic E3 ligase activity has not been proven.
Uniprot ID
Q9H9P5
Ensemble ID
ENST00000248104.11
HGNC ID
HGNC:14184

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C595(0.84)  LDD0533  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0216  AC100 HCT 116 C595(0.84)  LDD0533  [1]
 LDCM0217  AC101 HCT 116 C595(1.04)  LDD0534  [1]
 LDCM0218  AC102 HCT 116 C595(0.72)  LDD0535  [1]
 LDCM0219  AC103 HCT 116 C595(1.21)  LDD0536  [1]
 LDCM0220  AC104 HCT 116 C595(1.17)  LDD0537  [1]
 LDCM0221  AC105 HCT 116 C595(0.92)  LDD0538  [1]
 LDCM0222  AC106 HCT 116 C595(1.31)  LDD0539  [1]
 LDCM0223  AC107 HCT 116 C595(1.28)  LDD0540  [1]
 LDCM0224  AC108 HCT 116 C595(0.83)  LDD0541  [1]
 LDCM0225  AC109 HCT 116 C595(1.13)  LDD0542  [1]
 LDCM0227  AC110 HCT 116 C595(0.81)  LDD0544  [1]
 LDCM0228  AC111 HCT 116 C595(0.81)  LDD0545  [1]
 LDCM0229  AC112 HCT 116 C595(1.05)  LDD0546  [1]
 LDCM0263  AC143 HCT 116 C595(0.86)  LDD0580  [1]
 LDCM0264  AC144 HCT 116 C595(1.08)  LDD0581  [1]
 LDCM0265  AC145 HCT 116 C595(1.14)  LDD0582  [1]
 LDCM0266  AC146 HCT 116 C595(0.96)  LDD0583  [1]
 LDCM0267  AC147 HCT 116 C595(1.49)  LDD0584  [1]
 LDCM0268  AC148 HCT 116 C595(2.13)  LDD0585  [1]
 LDCM0269  AC149 HCT 116 C595(1.25)  LDD0586  [1]
 LDCM0271  AC150 HCT 116 C595(0.86)  LDD0588  [1]
 LDCM0272  AC151 HCT 116 C595(1.21)  LDD0589  [1]
 LDCM0273  AC152 HCT 116 C595(1.67)  LDD0590  [1]
 LDCM0274  AC153 HCT 116 C595(2.34)  LDD0591  [1]
 LDCM0621  AC154 HCT 116 C595(1.24)  LDD2158  [1]
 LDCM0622  AC155 HCT 116 C595(1.19)  LDD2159  [1]
 LDCM0623  AC156 HCT 116 C595(1.11)  LDD2160  [1]
 LDCM0624  AC157 HCT 116 C595(0.97)  LDD2161  [1]
 LDCM0296  AC35 HCT 116 C595(0.60)  LDD0613  [1]
 LDCM0297  AC36 HCT 116 C595(0.69)  LDD0614  [1]
 LDCM0298  AC37 HCT 116 C595(0.89)  LDD0615  [1]
 LDCM0299  AC38 HCT 116 C595(1.26)  LDD0616  [1]
 LDCM0300  AC39 HCT 116 C595(1.19)  LDD0617  [1]
 LDCM0302  AC40 HCT 116 C595(1.68)  LDD0619  [1]
 LDCM0303  AC41 HCT 116 C595(0.86)  LDD0620  [1]
 LDCM0304  AC42 HCT 116 C595(0.94)  LDD0621  [1]
 LDCM0305  AC43 HCT 116 C595(1.06)  LDD0622  [1]
 LDCM0306  AC44 HCT 116 C595(0.81)  LDD0623  [1]
 LDCM0307  AC45 HCT 116 C595(1.26)  LDD0624  [1]
 LDCM0320  AC57 HCT 116 C595(2.10)  LDD0637  [1]
 LDCM0321  AC58 HCT 116 C595(2.44)  LDD0638  [1]
 LDCM0322  AC59 HCT 116 C595(1.66)  LDD0639  [1]
 LDCM0324  AC60 HCT 116 C595(1.14)  LDD0641  [1]
 LDCM0325  AC61 HCT 116 C595(1.04)  LDD0642  [1]
 LDCM0326  AC62 HCT 116 C595(1.14)  LDD0643  [1]
 LDCM0327  AC63 HCT 116 C595(1.26)  LDD0644  [1]
 LDCM0328  AC64 HCT 116 C595(1.29)  LDD0645  [1]
 LDCM0329  AC65 HCT 116 C595(0.61)  LDD0646  [1]
 LDCM0330  AC66 HCT 116 C595(0.51)  LDD0647  [1]
 LDCM0331  AC67 HCT 116 C595(0.65)  LDD0648  [1]
 LDCM0349  AC83 HCT 116 C595(2.41)  LDD0666  [1]
 LDCM0350  AC84 HCT 116 C595(2.44)  LDD0667  [1]
 LDCM0351  AC85 HCT 116 C595(2.02)  LDD0668  [1]
 LDCM0352  AC86 HCT 116 C595(1.50)  LDD0669  [1]
 LDCM0353  AC87 HCT 116 C595(1.47)  LDD0670  [1]
 LDCM0354  AC88 HCT 116 C595(1.69)  LDD0671  [1]
 LDCM0355  AC89 HCT 116 C595(1.90)  LDD0672  [1]
 LDCM0357  AC90 HCT 116 C595(1.27)  LDD0674  [1]
 LDCM0358  AC91 HCT 116 C595(2.44)  LDD0675  [1]
 LDCM0359  AC92 HCT 116 C595(2.94)  LDD0676  [1]
 LDCM0360  AC93 HCT 116 C595(1.89)  LDD0677  [1]
 LDCM0361  AC94 HCT 116 C595(1.70)  LDD0678  [1]
 LDCM0362  AC95 HCT 116 C595(1.82)  LDD0679  [1]
 LDCM0363  AC96 HCT 116 C595(2.04)  LDD0680  [1]
 LDCM0364  AC97 HCT 116 C595(1.79)  LDD0681  [1]
 LDCM0365  AC98 HCT 116 C595(2.90)  LDD0682  [1]
 LDCM0366  AC99 HCT 116 C595(0.70)  LDD0683  [1]
 LDCM0387  CL117 HCT 116 C595(3.41)  LDD0704  [1]
 LDCM0388  CL118 HCT 116 C595(1.16)  LDD0705  [1]
 LDCM0389  CL119 HCT 116 C595(1.42)  LDD0706  [1]
 LDCM0391  CL120 HCT 116 C595(0.96)  LDD0708  [1]
 LDCM0396  CL125 HCT 116 C595(1.31)  LDD0713  [1]
 LDCM0397  CL126 HCT 116 C595(0.95)  LDD0714  [1]
 LDCM0398  CL127 HCT 116 C595(1.54)  LDD0715  [1]
 LDCM0399  CL128 HCT 116 C595(2.26)  LDD0716  [1]
 LDCM0022  KB02 HEK-293T C249(0.97)  LDD1492  [3]
 LDCM0023  KB03 HEK-293T C249(0.98)  LDD1497  [3]
 LDCM0024  KB05 OVCAR-4 C352(1.49)  LDD3382  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
DNA-directed RNA polymerases I and III subunit RPAC1 (POLR1C) Archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family O15160
Protein N-terminal glutamine amidohydrolase (NTAQ1) NTAQ1 family Q96HA8
Kallikrein-6 (KLK6) Peptidase S1 family Q92876
Ras-related protein Rab-26 (RAB26) Rab family Q9ULW5
Ras-related C3 botulinum toxin substrate 1 (RAC1) Rho family P63000
Protein-glutamine gamma-glutamyltransferase Z (TGM7) Transglutaminase family Q96PF1
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Alpha-crystallin A chain (CRYAA) Small heat shock protein (HSP20) family P02489
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Homeobox protein Hox-A1 (HOXA1) Antp homeobox family P49639
Other
Click To Hide/Show 11 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ataxin-1-like (ATXN1L) ATXN1 family P0C7T5
Beta-crystallin A2 (CRYBA2) Beta/gamma-crystallin family P53672
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2 (SMARCD2) SMARCD family Q92925
BAG family molecular chaperone regulator 3 (BAG3) . O95817
BAG family molecular chaperone regulator 4 (BAG4) . O95429
BTB/POZ domain-containing protein KCTD9 (KCTD9) . Q7L273
Calcium-binding protein 2 (CABP2) . Q9NPB3
Fibroblast growth factor receptor substrate 3 (FRS3) . O43559
Heterogeneous nuclear ribonucleoprotein F (HNRNPF) . P52597
Protein phosphatase 1 regulatory subunit 16A (PPP1R16A) . Q96I34
Rhombotin-1 (LMO1) . P25800

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840