Details of the Target

General Information of Target

Target ID LDTP11992
Target Name Acyl-CoA synthetase short-chain family member 3, mitochondrial (ACSS3)
Gene Name ACSS3
Gene ID 79611
Synonyms
Acyl-CoA synthetase short-chain family member 3, mitochondrial; EC 6.2.1.1; Acetate--CoA ligase 3; Acyl-CoA synthetase short-chain family member 3; Propionate--CoA ligase; EC 6.2.1.17
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPEEAGFPPAKRFRPGSGPPSRAGSFPPGRQVVMLLTAGSGGRGGGGGRRQQPPLAQPSA
SPYPEAVELQRRSLPIFQARGQLLAQLRNLDNAVLIGETGSGKTTQIPQYLYEGGISRQG
IIAVTQPRRVAAISLATRVSDEKRTELGKLVGYTVRFDDVTSEDTRIKFLTDGMLLREAI
SDSLLRKYSCVILDEAHERTIHTDVLFGVVKAAQKRRKELGKLPLKVIVMSATMDVDLFS
QYFNGAPVLYLEGRQHPIQVFYTKQPQNDYLHAALVSVFQIHQEAPSSQDILVFLTGQEE
IEAMSKTCRDIAKHLPDGCPAMLVLPLYASLPYAQQLRVFQGAPKGYRKVIISTNIAETS
ITITGIKYVVDTGMVKAKKYNPDSGLEVLAVQRVSKTQAWQRTGRAGREDSGICYRLYTE
DEFEKFDKMTVPEIQRCNLASVMLQLLAMKVPNVLTFDFMSKPSPDHIQAAIAQLDLLGA
LEHKDDQLTLTPMGRKMAAFPLEPKFAKTILMSPKFHCTEEILTIVSLLSVDSVLHNPPS
RREEVQGVRKKFISSEGDHMTLLNIYRTFKNLGGNKDWCKENFVNSKNMTLVAEVRAQLR
DICLKMSMPIASSRGDVESVRRCLAHSLFMSTAELQPDGTYATTDTHQPVAIHPSSVLFH
CKPACVVYTELLYTNKCYMRDLCVIDAQWLYEAAPEYFRRKLRTARN
Target Bioclass
Enzyme
Family
ATP-dependent AMP-binding enzyme family
Subcellular location
Mitochondrion matrix
Function Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids. Propionate is the preferred substrate. Can utilize acetate and butyrate with a much lower affinity.
Uniprot ID
Q9H6R3
Ensemble ID
ENST00000548058.6
HGNC ID
HGNC:24723

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A172 SNV: p.E681G DBIA    Probe Info 
A3KAW SNV: p.I575M .
COLO679 SNV: p.S25F DBIA    Probe Info 
COLO800 SNV: p.E681K DBIA    Probe Info 
ETK1 Substitution: p.A585V .
HCC15 SNV: p.A686E .
IPC298 SNV: p.H195Y DBIA    Probe Info 
JURKAT SNV: p.M260T .
LNCaP clone FGC SNV: p.L599I .
MELHO SNV: p.K167E .
MELJUSO SNV: p.P532S .
MEWO SNV: p.S349F; p.S460L DBIA    Probe Info 
MOLT4 SNV: p.A568T .
RKO SNV: p.Y295H .
TE11 SNV: p.K662E .
TE4 SNV: p.S647Y DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
YN-1
 Probe Info  		100.00  LDD0444  [1]
IPM
 Probe Info  		C428(0.00); C602(0.00); C498(0.00); C285(0.00)  LDD0241  [2]
Probe 1
 Probe Info  		Y234(81.38)  LDD3495  [3]
DBIA
 Probe Info  		C498(1.45)  LDD3312  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [5]
NAIA_5
 Probe Info  		N.A.  LDD2225  [6]
W1
 Probe Info  		C602(0.00); D72(0.00); S69(0.00); H65(0.00)  LDD0236  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C498(0.90)  LDD1507  [7]
 LDCM0215  AC10 HEK-293T C498(0.94)  LDD1508  [7]
 LDCM0237  AC12 HEK-293T C498(1.06)  LDD1510  [7]
 LDCM0259  AC14 HEK-293T C498(1.00)  LDD1512  [7]
 LDCM0270  AC15 HEK-293T C498(0.98)  LDD1513  [7]
 LDCM0276  AC17 HEK-293T C498(0.93)  LDD1515  [7]
 LDCM0277  AC18 HEK-293T C498(0.96)  LDD1516  [7]
 LDCM0279  AC2 HEK-293T C498(1.05)  LDD1518  [7]
 LDCM0280  AC20 HEK-293T C498(1.05)  LDD1519  [7]
 LDCM0281  AC21 HEK-293T C498(0.95)  LDD1520  [7]
 LDCM0282  AC22 HEK-293T C498(0.93)  LDD1521  [7]
 LDCM0283  AC23 HEK-293T C498(1.01)  LDD1522  [7]
 LDCM0285  AC25 HEK-293T C498(0.96)  LDD1524  [7]
 LDCM0286  AC26 HEK-293T C498(0.94)  LDD1525  [7]
 LDCM0288  AC28 HEK-293T C498(1.01)  LDD1527  [7]
 LDCM0289  AC29 HEK-293T C498(1.01)  LDD1528  [7]
 LDCM0291  AC30 HEK-293T C498(1.04)  LDD1530  [7]
 LDCM0292  AC31 HEK-293T C498(0.98)  LDD1531  [7]
 LDCM0294  AC33 HEK-293T C498(0.83)  LDD1533  [7]
 LDCM0295  AC34 HEK-293T C498(0.94)  LDD1534  [7]
 LDCM0297  AC36 HEK-293T C498(0.97)  LDD1536  [7]
 LDCM0298  AC37 HEK-293T C498(1.03)  LDD1537  [7]
 LDCM0299  AC38 HEK-293T C498(1.07)  LDD1538  [7]
 LDCM0300  AC39 HEK-293T C498(0.98)  LDD1539  [7]
 LDCM0301  AC4 HEK-293T C498(0.99)  LDD1540  [7]
 LDCM0303  AC41 HEK-293T C498(1.04)  LDD1542  [7]
 LDCM0304  AC42 HEK-293T C498(0.99)  LDD1543  [7]
 LDCM0306  AC44 HEK-293T C498(0.99)  LDD1545  [7]
 LDCM0307  AC45 HEK-293T C498(1.02)  LDD1546  [7]
 LDCM0308  AC46 HEK-293T C498(1.02)  LDD1547  [7]
 LDCM0309  AC47 HEK-293T C498(0.99)  LDD1548  [7]
 LDCM0311  AC49 HEK-293T C498(0.97)  LDD1550  [7]
 LDCM0312  AC5 HEK-293T C498(1.03)  LDD1551  [7]
 LDCM0313  AC50 HEK-293T C498(0.95)  LDD1552  [7]
 LDCM0315  AC52 HEK-293T C498(0.99)  LDD1554  [7]
 LDCM0316  AC53 HEK-293T C498(1.03)  LDD1555  [7]
 LDCM0317  AC54 HEK-293T C498(1.06)  LDD1556  [7]
 LDCM0318  AC55 HEK-293T C498(1.02)  LDD1557  [7]
 LDCM0320  AC57 HEK-293T C498(0.93)  LDD1559  [7]
 LDCM0321  AC58 HEK-293T C498(0.95)  LDD1560  [7]
 LDCM0323  AC6 HEK-293T C498(1.00)  LDD1562  [7]
 LDCM0324  AC60 HEK-293T C498(1.02)  LDD1563  [7]
 LDCM0325  AC61 HEK-293T C498(0.99)  LDD1564  [7]
 LDCM0326  AC62 HEK-293T C498(1.03)  LDD1565  [7]
 LDCM0327  AC63 HEK-293T C498(0.96)  LDD1566  [7]
 LDCM0334  AC7 HEK-293T C498(0.99)  LDD1568  [7]
 LDCM0248  AKOS034007472 HEK-293T C498(1.08)  LDD1511  [7]
 LDCM0356  AKOS034007680 HEK-293T C498(0.97)  LDD1570  [7]
 LDCM0367  CL1 HEK-293T C498(0.94)  LDD1571  [7]
 LDCM0368  CL10 HEK-293T C498(0.63)  LDD1572  [7]
 LDCM0369  CL100 HEK-293T C498(0.93)  LDD1573  [7]
 LDCM0370  CL101 HEK-293T C498(1.11)  LDD1574  [7]
 LDCM0372  CL103 HEK-293T C498(0.93)  LDD1576  [7]
 LDCM0373  CL104 HEK-293T C498(0.96)  LDD1577  [7]
 LDCM0374  CL105 HEK-293T C498(1.00)  LDD1578  [7]
 LDCM0376  CL107 HEK-293T C498(0.93)  LDD1580  [7]
 LDCM0377  CL108 HEK-293T C498(0.95)  LDD1581  [7]
 LDCM0378  CL109 HEK-293T C498(0.93)  LDD1582  [7]
 LDCM0379  CL11 HEK-293T C498(1.02)  LDD1583  [7]
 LDCM0381  CL111 HEK-293T C498(0.91)  LDD1585  [7]
 LDCM0382  CL112 HEK-293T C498(0.98)  LDD1586  [7]
 LDCM0383  CL113 HEK-293T C498(1.08)  LDD1587  [7]
 LDCM0385  CL115 HEK-293T C498(0.96)  LDD1589  [7]
 LDCM0386  CL116 HEK-293T C498(0.97)  LDD1590  [7]
 LDCM0387  CL117 HEK-293T C498(1.09)  LDD1591  [7]
 LDCM0389  CL119 HEK-293T C498(1.05)  LDD1593  [7]
 LDCM0391  CL120 HEK-293T C498(1.08)  LDD1595  [7]
 LDCM0392  CL121 HEK-293T C498(1.03)  LDD1596  [7]
 LDCM0394  CL123 HEK-293T C498(0.92)  LDD1598  [7]
 LDCM0395  CL124 HEK-293T C498(1.08)  LDD1599  [7]
 LDCM0396  CL125 HEK-293T C498(1.09)  LDD1600  [7]
 LDCM0398  CL127 HEK-293T C498(0.95)  LDD1602  [7]
 LDCM0399  CL128 HEK-293T C498(0.95)  LDD1603  [7]
 LDCM0400  CL13 HEK-293T C498(0.97)  LDD1604  [7]
 LDCM0402  CL15 HEK-293T C498(0.94)  LDD1606  [7]
 LDCM0403  CL16 HEK-293T C498(0.97)  LDD1607  [7]
 LDCM0404  CL17 HEK-293T C498(0.77)  LDD1608  [7]
 LDCM0405  CL18 HEK-293T C498(0.99)  LDD1609  [7]
 LDCM0408  CL20 HEK-293T C498(0.99)  LDD1612  [7]
 LDCM0409  CL21 HEK-293T C498(1.00)  LDD1613  [7]
 LDCM0410  CL22 HEK-293T C498(0.98)  LDD1614  [7]
 LDCM0411  CL23 HEK-293T C498(1.04)  LDD1615  [7]
 LDCM0413  CL25 HEK-293T C498(0.96)  LDD1617  [7]
 LDCM0415  CL27 HEK-293T C498(0.95)  LDD1619  [7]
 LDCM0416  CL28 HEK-293T C498(0.99)  LDD1620  [7]
 LDCM0417  CL29 HEK-293T C498(1.04)  LDD1621  [7]
 LDCM0418  CL3 HEK-293T C498(0.91)  LDD1622  [7]
 LDCM0419  CL30 HEK-293T C498(0.99)  LDD1623  [7]
 LDCM0421  CL32 HEK-293T C498(1.03)  LDD1625  [7]
 LDCM0422  CL33 HEK-293T C498(0.96)  LDD1626  [7]
 LDCM0423  CL34 HEK-293T C498(1.01)  LDD1627  [7]
 LDCM0424  CL35 HEK-293T C498(1.12)  LDD1628  [7]
 LDCM0426  CL37 HEK-293T C498(1.02)  LDD1630  [7]
 LDCM0428  CL39 HEK-293T C498(1.05)  LDD1632  [7]
 LDCM0429  CL4 HEK-293T C498(0.91)  LDD1633  [7]
 LDCM0430  CL40 HEK-293T C498(0.96)  LDD1634  [7]
 LDCM0431  CL41 HEK-293T C498(0.83)  LDD1635  [7]
 LDCM0432  CL42 HEK-293T C498(1.00)  LDD1636  [7]
 LDCM0434  CL44 HEK-293T C498(1.07)  LDD1638  [7]
 LDCM0435  CL45 HEK-293T C498(1.00)  LDD1639  [7]
 LDCM0436  CL46 HEK-293T C498(0.96)  LDD1640  [7]
 LDCM0437  CL47 HEK-293T C498(1.03)  LDD1641  [7]
 LDCM0439  CL49 HEK-293T C498(0.94)  LDD1643  [7]
 LDCM0440  CL5 HEK-293T C498(0.90)  LDD1644  [7]
 LDCM0443  CL52 HEK-293T C498(0.97)  LDD1646  [7]
 LDCM0444  CL53 HEK-293T C498(0.73)  LDD1647  [7]
 LDCM0445  CL54 HEK-293T C498(0.86)  LDD1648  [7]
 LDCM0447  CL56 HEK-293T C498(1.04)  LDD1650  [7]
 LDCM0448  CL57 HEK-293T C498(0.96)  LDD1651  [7]
 LDCM0449  CL58 HEK-293T C498(0.98)  LDD1652  [7]
 LDCM0450  CL59 HEK-293T C498(0.96)  LDD1653  [7]
 LDCM0451  CL6 HEK-293T C498(0.96)  LDD1654  [7]
 LDCM0453  CL61 HEK-293T C498(1.03)  LDD1656  [7]
 LDCM0455  CL63 HEK-293T C498(1.06)  LDD1658  [7]
 LDCM0456  CL64 HEK-293T C498(0.96)  LDD1659  [7]
 LDCM0457  CL65 HEK-293T C498(0.89)  LDD1660  [7]
 LDCM0458  CL66 HEK-293T C498(0.98)  LDD1661  [7]
 LDCM0460  CL68 HEK-293T C498(1.05)  LDD1663  [7]
 LDCM0461  CL69 HEK-293T C498(1.08)  LDD1664  [7]
 LDCM0463  CL70 HEK-293T C498(1.04)  LDD1666  [7]
 LDCM0464  CL71 HEK-293T C498(1.08)  LDD1667  [7]
 LDCM0466  CL73 HEK-293T C498(0.98)  LDD1669  [7]
 LDCM0469  CL76 HEK-293T C498(1.04)  LDD1672  [7]
 LDCM0470  CL77 HEK-293T C498(0.93)  LDD1673  [7]
 LDCM0471  CL78 HEK-293T C498(1.03)  LDD1674  [7]
 LDCM0473  CL8 HEK-293T C498(0.83)  LDD1676  [7]
 LDCM0474  CL80 HEK-293T C498(1.05)  LDD1677  [7]
 LDCM0475  CL81 HEK-293T C498(1.14)  LDD1678  [7]
 LDCM0476  CL82 HEK-293T C498(1.05)  LDD1679  [7]
 LDCM0477  CL83 HEK-293T C498(0.98)  LDD1680  [7]
 LDCM0479  CL85 HEK-293T C498(1.15)  LDD1682  [7]
 LDCM0481  CL87 HEK-293T C498(1.00)  LDD1684  [7]
 LDCM0482  CL88 HEK-293T C498(0.96)  LDD1685  [7]
 LDCM0483  CL89 HEK-293T C498(1.05)  LDD1686  [7]
 LDCM0484  CL9 HEK-293T C498(1.05)  LDD1687  [7]
 LDCM0485  CL90 HEK-293T C498(0.85)  LDD1688  [7]
 LDCM0487  CL92 HEK-293T C498(0.92)  LDD1690  [7]
 LDCM0488  CL93 HEK-293T C498(1.00)  LDD1691  [7]
 LDCM0489  CL94 HEK-293T C498(0.96)  LDD1692  [7]
 LDCM0490  CL95 HEK-293T C498(0.88)  LDD1693  [7]
 LDCM0492  CL97 HEK-293T C498(0.93)  LDD1695  [7]
 LDCM0494  CL99 HEK-293T C498(0.93)  LDD1697  [7]
 LDCM0495  E2913 HEK-293T C498(0.92)  LDD1698  [7]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C498(1.06)  LDD1702  [8]
 LDCM0468  Fragment33 HEK-293T C498(1.04)  LDD1671  [7]
 LDCM0022  KB02 HEK-293T C498(1.04)  LDD1492  [7]
 LDCM0023  KB03 HEK-293T C498(1.01)  LDD1497  [7]
 LDCM0024  KB05 HMCB C498(1.45)  LDD3312  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Optineurin (OPTN) . Q96CV9

References

1 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
2 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
3 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
6 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
7 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
8 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761