Details of the Target

General Information of Target

Target ID LDTP11988
Target Name Threonine aspartase 1 (TASP1)
Gene Name TASP1
Gene ID 55617
Synonyms
C20orf13; Threonine aspartase 1; Taspase-1; EC 3.4.25.-) [Cleaved into: Threonine aspartase subunit alpha; Threonine aspartase subunit beta]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MASPAPPEHAEEGCPAPAAEEQAPPSPPPPQASPAERQQQEEEAQEAGAAEGAGLQVEEA
AGRAAAAVTWLLGEPVLWLGCRADELLSWKRPLRSLLGFVAANLLFWFLALTPWRVYHLI
SVMILGRVIMQIIKDMVLSRTRGAQLWRSLSESWEVINSKPDERPRLSHCIAESWMNFSI
FLQEMSLFKQQSPGKFCLLVCSVCTFFTILGSYIPGVILSYLLLLCAFLCPLFKCNDIGQ
KIYSKIKSVLLKLDFGIGEYINQKKRERSEADKEKSHKDDSELDFSALCPKISLTVAAKE
LSVSDTDVSEVSWTDNGTFNLSEGYTPQTDTSDDLDRPSEEVFSRDLSDFPSLENGMGTN
DEDELSLGLPTELKRKKEQLDSGHRPSKETQSAAGLTLPLNSDQTFHLMSNLAGDVITAA
VTAAIKDQLEGVQQALSQAAPIPEEDTDTEEGDDFELLDQSELDQIESELGLTQDQEAEA
QQNKKSSGFLSNLLGGH
Target Bioclass
Enzyme
Family
Ntn-hydrolase family
Function
Protease responsible for KMT2A/MLL1 processing and activation. It also activates KMT2D/MLL2. Through substrate activation, it controls the expression of HOXA genes, and the expression of key cell cycle regulators including CCNA1, CCNB1, CCNE1 and CDKN2A.
Uniprot ID
Q9H6P5
Ensemble ID
ENST00000337743.9
HGNC ID
HGNC:15859
ChEMBL ID
CHEMBL6153

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 SNV: p.H63Y; p.D337V .
HGC27 SNV: p.P331L .
HT SNV: p.E4V .
Ishikawa (Heraklio) 02 ER SNV: p.E243K .
JURKAT SNV: p.A72V .
MELJUSO SNV: p.E31G .
SKES1 SNV: p.F332I .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C351(1.32)  LDD0078  [1]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [2]
Lodoacetamide azide
 Probe Info  		C351(0.00); C148(0.00)  LDD0037  [2]
NAIA_5
 Probe Info  		N.A.  LDD2224  [3]
TFBX
 Probe Info  		N.A.  LDD0148  [4]
W1
 Probe Info  		N.A.  LDD0236  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C351(20.00)  LDD0371  [6]
 LDCM0214  AC1 HEK-293T C351(0.93)  LDD1507  [7]
 LDCM0237  AC12 HEK-293T C351(1.02)  LDD1510  [7]
 LDCM0276  AC17 HCT 116 C351(1.06)  LDD0593  [1]
 LDCM0277  AC18 HCT 116 C351(1.21)  LDD0594  [1]
 LDCM0278  AC19 HCT 116 C351(1.09)  LDD0595  [1]
 LDCM0280  AC20 HCT 116 C351(1.02)  LDD0597  [1]
 LDCM0281  AC21 HCT 116 C351(1.34)  LDD0598  [1]
 LDCM0282  AC22 HCT 116 C351(0.93)  LDD0599  [1]
 LDCM0283  AC23 HCT 116 C351(0.99)  LDD0600  [1]
 LDCM0284  AC24 HCT 116 C351(1.04)  LDD0601  [1]
 LDCM0285  AC25 HCT 116 C351(1.21)  LDD0602  [1]
 LDCM0286  AC26 HCT 116 C351(2.73)  LDD0603  [1]
 LDCM0287  AC27 HCT 116 C351(1.69)  LDD0604  [1]
 LDCM0288  AC28 HCT 116 C351(1.39)  LDD0605  [1]
 LDCM0289  AC29 HCT 116 C351(1.61)  LDD0606  [1]
 LDCM0291  AC30 HCT 116 C351(1.50)  LDD0608  [1]
 LDCM0292  AC31 HCT 116 C351(1.34)  LDD0609  [1]
 LDCM0293  AC32 HCT 116 C351(1.84)  LDD0610  [1]
 LDCM0294  AC33 HCT 116 C351(1.91)  LDD0611  [1]
 LDCM0295  AC34 HCT 116 C351(1.67)  LDD0612  [1]
 LDCM0297  AC36 HEK-293T C351(0.92)  LDD1536  [7]
 LDCM0298  AC37 HEK-293T C351(0.86)  LDD1537  [7]
 LDCM0301  AC4 HEK-293T C351(0.97)  LDD1540  [7]
 LDCM0302  AC40 HEK-293T C351(0.85)  LDD1541  [7]
 LDCM0303  AC41 HEK-293T C351(0.91)  LDD1542  [7]
 LDCM0306  AC44 HEK-293T C351(1.16)  LDD1545  [7]
 LDCM0307  AC45 HEK-293T C351(1.09)  LDD1546  [7]
 LDCM0310  AC48 HEK-293T C351(0.73)  LDD1549  [7]
 LDCM0311  AC49 HEK-293T C351(0.98)  LDD1550  [7]
 LDCM0312  AC5 HEK-293T C351(1.03)  LDD1551  [7]
 LDCM0315  AC52 HEK-293T C351(0.92)  LDD1554  [7]
 LDCM0316  AC53 HEK-293T C351(1.28)  LDD1555  [7]
 LDCM0319  AC56 HEK-293T C351(0.93)  LDD1558  [7]
 LDCM0320  AC57 HEK-293T C351(0.88)  LDD1559  [7]
 LDCM0324  AC60 HEK-293T C351(0.93)  LDD1563  [7]
 LDCM0325  AC61 HEK-293T C351(1.00)  LDD1564  [7]
 LDCM0328  AC64 HEK-293T C351(0.92)  LDD1567  [7]
 LDCM0345  AC8 HEK-293T C351(0.84)  LDD1569  [7]
 LDCM0349  AC83 HCT 116 C351(1.14)  LDD0666  [1]
 LDCM0350  AC84 HCT 116 C351(1.11)  LDD0667  [1]
 LDCM0351  AC85 HCT 116 C351(1.21)  LDD0668  [1]
 LDCM0352  AC86 HCT 116 C351(1.14)  LDD0669  [1]
 LDCM0353  AC87 HCT 116 C351(0.95)  LDD0670  [1]
 LDCM0354  AC88 HCT 116 C351(0.94)  LDD0671  [1]
 LDCM0355  AC89 HCT 116 C351(1.23)  LDD0672  [1]
 LDCM0357  AC90 HCT 116 C351(1.41)  LDD0674  [1]
 LDCM0358  AC91 HCT 116 C351(1.28)  LDD0675  [1]
 LDCM0359  AC92 HCT 116 C351(1.11)  LDD0676  [1]
 LDCM0360  AC93 HCT 116 C351(1.00)  LDD0677  [1]
 LDCM0361  AC94 HCT 116 C351(1.41)  LDD0678  [1]
 LDCM0362  AC95 HCT 116 C351(1.24)  LDD0679  [1]
 LDCM0363  AC96 HCT 116 C351(1.53)  LDD0680  [1]
 LDCM0364  AC97 HCT 116 C351(1.32)  LDD0681  [1]
 LDCM0248  AKOS034007472 HEK-293T C351(0.94)  LDD1511  [7]
 LDCM0356  AKOS034007680 HEK-293T C351(0.89)  LDD1570  [7]
 LDCM0275  AKOS034007705 HEK-293T C351(0.84)  LDD1514  [7]
 LDCM0020  ARS-1620 HCC44 C351(1.32)  LDD0078  [1]
 LDCM0367  CL1 HEK-293T C351(0.83)  LDD1571  [7]
 LDCM0370  CL101 HEK-293T C351(1.08)  LDD1574  [7]
 LDCM0371  CL102 HEK-293T C351(0.95)  LDD1575  [7]
 LDCM0372  CL103 HEK-293T C351(1.00)  LDD1576  [7]
 LDCM0374  CL105 HCT 116 C351(1.09)  LDD0691  [1]
 LDCM0375  CL106 HCT 116 C351(1.23)  LDD0692  [1]
 LDCM0376  CL107 HCT 116 C351(1.06)  LDD0693  [1]
 LDCM0377  CL108 HCT 116 C351(1.68)  LDD0694  [1]
 LDCM0378  CL109 HCT 116 C351(0.96)  LDD0695  [1]
 LDCM0380  CL110 HCT 116 C351(1.30)  LDD0697  [1]
 LDCM0381  CL111 HCT 116 C351(1.32)  LDD0698  [1]
 LDCM0382  CL112 HCT 116 C351(1.15)  LDD0699  [1]
 LDCM0383  CL113 HCT 116 C351(1.72)  LDD0700  [1]
 LDCM0384  CL114 HCT 116 C351(1.34)  LDD0701  [1]
 LDCM0385  CL115 HCT 116 C351(1.56)  LDD0702  [1]
 LDCM0386  CL116 HCT 116 C351(1.56)  LDD0703  [1]
 LDCM0387  CL117 HEK-293T C351(0.98)  LDD1591  [7]
 LDCM0388  CL118 HEK-293T C351(1.05)  LDD1592  [7]
 LDCM0389  CL119 HEK-293T C351(1.36)  LDD1593  [7]
 LDCM0390  CL12 HEK-293T C351(0.78)  LDD1594  [7]
 LDCM0392  CL121 HEK-293T C351(1.11)  LDD1596  [7]
 LDCM0393  CL122 HEK-293T C351(1.03)  LDD1597  [7]
 LDCM0394  CL123 HEK-293T C351(1.39)  LDD1598  [7]
 LDCM0396  CL125 HEK-293T C351(1.04)  LDD1600  [7]
 LDCM0397  CL126 HEK-293T C351(0.91)  LDD1601  [7]
 LDCM0398  CL127 HEK-293T C351(1.08)  LDD1602  [7]
 LDCM0400  CL13 HEK-293T C351(0.97)  LDD1604  [7]
 LDCM0401  CL14 HEK-293T C351(1.20)  LDD1605  [7]
 LDCM0402  CL15 HEK-293T C351(0.97)  LDD1606  [7]
 LDCM0404  CL17 HCT 116 C351(1.38)  LDD0721  [1]
 LDCM0405  CL18 HCT 116 C351(1.07)  LDD0722  [1]
 LDCM0406  CL19 HCT 116 C351(1.12)  LDD0723  [1]
 LDCM0407  CL2 HEK-293T C351(1.09)  LDD1611  [7]
 LDCM0408  CL20 HCT 116 C351(1.40)  LDD0725  [1]
 LDCM0409  CL21 HCT 116 C351(1.28)  LDD0726  [1]
 LDCM0410  CL22 HCT 116 C351(0.97)  LDD0727  [1]
 LDCM0411  CL23 HCT 116 C351(1.03)  LDD0728  [1]
 LDCM0412  CL24 HCT 116 C351(1.16)  LDD0729  [1]
 LDCM0413  CL25 HCT 116 C351(1.22)  LDD0730  [1]
 LDCM0414  CL26 HCT 116 C351(1.14)  LDD0731  [1]
 LDCM0415  CL27 HCT 116 C351(1.10)  LDD0732  [1]
 LDCM0416  CL28 HCT 116 C351(1.22)  LDD0733  [1]
 LDCM0417  CL29 HCT 116 C351(1.10)  LDD0734  [1]
 LDCM0418  CL3 HEK-293T C351(0.90)  LDD1622  [7]
 LDCM0419  CL30 HCT 116 C351(1.25)  LDD0736  [1]
 LDCM0420  CL31 HCT 116 C351(1.24)  LDD0737  [1]
 LDCM0421  CL32 HEK-293T C351(1.14)  LDD1625  [7]
 LDCM0422  CL33 HEK-293T C351(0.95)  LDD1626  [7]
 LDCM0425  CL36 HEK-293T C351(1.00)  LDD1629  [7]
 LDCM0426  CL37 HEK-293T C351(1.01)  LDD1630  [7]
 LDCM0428  CL39 HEK-293T C351(1.12)  LDD1632  [7]
 LDCM0431  CL41 HEK-293T C351(0.77)  LDD1635  [7]
 LDCM0434  CL44 HEK-293T C351(1.05)  LDD1638  [7]
 LDCM0435  CL45 HEK-293T C351(0.84)  LDD1639  [7]
 LDCM0438  CL48 HEK-293T C351(1.14)  LDD1642  [7]
 LDCM0439  CL49 HEK-293T C351(0.94)  LDD1643  [7]
 LDCM0440  CL5 HEK-293T C351(1.36)  LDD1644  [7]
 LDCM0441  CL50 HEK-293T C351(1.05)  LDD1645  [7]
 LDCM0444  CL53 HEK-293T C351(0.83)  LDD1647  [7]
 LDCM0447  CL56 HEK-293T C351(0.99)  LDD1650  [7]
 LDCM0448  CL57 HEK-293T C351(1.13)  LDD1651  [7]
 LDCM0452  CL60 HEK-293T C351(1.12)  LDD1655  [7]
 LDCM0453  CL61 HEK-293T C351(0.85)  LDD1656  [7]
 LDCM0454  CL62 HEK-293T C351(0.96)  LDD1657  [7]
 LDCM0455  CL63 HEK-293T C351(0.94)  LDD1658  [7]
 LDCM0457  CL65 HEK-293T C351(0.96)  LDD1660  [7]
 LDCM0460  CL68 HEK-293T C351(0.97)  LDD1663  [7]
 LDCM0461  CL69 HEK-293T C351(1.04)  LDD1664  [7]
 LDCM0465  CL72 HEK-293T C351(0.99)  LDD1668  [7]
 LDCM0466  CL73 HEK-293T C351(0.88)  LDD1669  [7]
 LDCM0467  CL74 HEK-293T C351(1.08)  LDD1670  [7]
 LDCM0470  CL77 HEK-293T C351(1.27)  LDD1673  [7]
 LDCM0473  CL8 HEK-293T C351(0.90)  LDD1676  [7]
 LDCM0474  CL80 HEK-293T C351(1.15)  LDD1677  [7]
 LDCM0475  CL81 HEK-293T C351(1.55)  LDD1678  [7]
 LDCM0478  CL84 HEK-293T C351(1.21)  LDD1681  [7]
 LDCM0479  CL85 HEK-293T C351(1.03)  LDD1682  [7]
 LDCM0480  CL86 HEK-293T C351(1.04)  LDD1683  [7]
 LDCM0481  CL87 HEK-293T C351(0.87)  LDD1684  [7]
 LDCM0483  CL89 HEK-293T C351(0.98)  LDD1686  [7]
 LDCM0484  CL9 HEK-293T C351(1.07)  LDD1687  [7]
 LDCM0487  CL92 HEK-293T C351(0.98)  LDD1690  [7]
 LDCM0488  CL93 HEK-293T C351(1.11)  LDD1691  [7]
 LDCM0491  CL96 HEK-293T C351(0.61)  LDD1694  [7]
 LDCM0492  CL97 HEK-293T C351(0.97)  LDD1695  [7]
 LDCM0493  CL98 HEK-293T C351(1.02)  LDD1696  [7]
 LDCM0494  CL99 HEK-293T C351(0.85)  LDD1697  [7]
 LDCM0495  E2913 HEK-293T C351(1.27)  LDD1698  [7]
 LDCM0468  Fragment33 HEK-293T C351(0.89)  LDD1671  [7]
 LDCM0427  Fragment51 HEK-293T C351(0.94)  LDD1631  [7]
 LDCM0022  KB02 HEK-293T C351(1.06)  LDD1492  [7]
 LDCM0023  KB03 HEK-293T C351(0.99)  LDD1497  [7]
 LDCM0024  KB05 HMCB C148(1.28)  LDD3312  [8]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Threonine aspartase 1 (TASP1) Ntn-hydrolase family Q9H6P5

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
5 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
6 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
7 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
8 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840