Details of the Target

General Information of Target

Target ID LDTP11711
Target Name Kelch-like protein 25 (KLHL25)
Gene Name KLHL25
Gene ID 64410
Synonyms
ENC2; Kelch-like protein 25; Ectoderm-neural cortex protein 2; ENC-2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MKDQQTVIMTECTSLQFVSPFAFEAMQKVDVVCLASLSDPELRLLLPCLVRMALCAPADQ
SQSWAQDKKLILRLLSGVEAVNSIVALLSVDFHALEQDASKEQQLRHKLGGGSGESILVS
QLQHGLTLEFEHSDSPRRLRLVLSELLAIMNKVSESNGEFFFKSSELFESPVYLEEAADV
LCILQAELPSLLPIVDVAEALLHVRNGAWFLCLLVANVPDSFNEVCRGLIKNGERQDEES
LGGRRRTDALRFLCKMNPSQALKVRGMVVEECHLPGLGVALTLDHTKNEACEDGVSDLVC
FVSGLLLGTNAKVRTWFGTFIRNGQQRKRETSSSVLWQMRRQLLLELMGILPTVRSTRIV
EEADVDMEPNVSVYSGLKEEHVVKASALLRLYCALMGIAGLKPTEEEAEQLLQLMTSRPP
ATPAGVRFVSLSFCMLLAFSTLVSTPEQEQLMVVWLSWMIKEEAYFESTSGVSASFGEML
LLVAMYFHSNQLSAIIDLVCSTLGMKIVIKPSSLSRMKTIFTQEIFTEQVVTAHAVRVPV
TSNLSANITGFLPIHCIYQLLRSRSFTKHKVSIKDWIYRQLCETSTPLHPQLLPLIDVYI
NSILTPASKSNPEATNQPVTEQEILNIFQGVIGGDNIRLNQRFSITAQLLVLYYILSYEE
ALLANTKTLAAMQRKPKSYSSSLMDQIPIKFLIRQAQGLQQELGGLHSALLRLLATNYPH
LCIVDDWICEEEITGTDALLRRMLLTNNAKNHSPKQLQEAFSAVPVNNTQVMQIIEHLTL
LSASELIPYAEVLTSNMSQLLNSGVPRRILQTVNKLWMVLNTVMPRRLWVMTVNALQPSI
KFVRQQKYTQNDLMIDPLIVLRCDQRVHRCPPLMDITLHMLNGYLLASKAYLSAHLKETE
QDRPSQNNTIGLVGQTDAPEVTREELKNALLAAQDSAAVQILLEICLPTEEEKANGVNPD
SLLRNVQSVITTSAPNKGMEEGEDNLLCNLREVQCLICCLLHQMYIADPNIAKLVHFQGY
PCELLPLTVAGIPSMHICLDFIPELIAQPELEKQIFAIQLLSHLCIQYALPKSLSVARLA
VNVMGTLLTVLTQAKRYAFFMPTLPSLVSFCRAFPPLYEDIMSLLIQIGQVCASDVATQT
RDIDPIITRLQQIKEKPSGWSQICKDSSYKNGSRDTGSMDPDVQLCHCIERTVIEIINMS
VSGI
Target Bioclass
Other
Function
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex involved in various processes, such as translation homeostasis and lipid synthesis. The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1 (4E-BP1): ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. The BCR(KLHL25) complex does not target EIF4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E. The BCR(KLHL25) complex also acts as a regulator of lipid synthesis by mediating ubiquitination and degradation of ACLY, thereby inhibiting lipid synthesis. BCR(KLHL25)-mediated degradation of ACLY promotes fatty acid oxidation and is required for differentiation of inducible regulatory T (iTreg) cells.
Uniprot ID
Q9H0H3
Ensemble ID
ENST00000337975.6
HGNC ID
HGNC:25732

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info  		C43(10.00)  LDD2157  [1]
DBIA
 Probe Info  		C478(23.38)  LDD0209  [2]
IPM
 Probe Info  		N.A.  LDD2156  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C287(0.98)  LDD1508  [4]
 LDCM0226  AC11 HEK-293T C31(1.29)  LDD1509  [4]
 LDCM0259  AC14 HEK-293T C340(0.96); C287(0.88)  LDD1512  [4]
 LDCM0270  AC15 HEK-293T C287(1.03)  LDD1513  [4]
 LDCM0277  AC18 HEK-293T C287(0.87)  LDD1516  [4]
 LDCM0278  AC19 HEK-293T C31(1.36)  LDD1517  [4]
 LDCM0279  AC2 HEK-293T C287(0.91)  LDD1518  [4]
 LDCM0282  AC22 HEK-293T C340(0.86); C287(0.75)  LDD1521  [4]
 LDCM0283  AC23 HEK-293T C287(1.10)  LDD1522  [4]
 LDCM0284  AC24 HEK-293T C340(1.03)  LDD1523  [4]
 LDCM0286  AC26 HEK-293T C287(0.91)  LDD1525  [4]
 LDCM0287  AC27 HEK-293T C31(0.96)  LDD1526  [4]
 LDCM0290  AC3 HEK-293T C31(0.88)  LDD1529  [4]
 LDCM0291  AC30 HEK-293T C340(0.97); C287(0.88)  LDD1530  [4]
 LDCM0292  AC31 HEK-293T C287(1.17)  LDD1531  [4]
 LDCM0293  AC32 HEK-293T C340(1.07)  LDD1532  [4]
 LDCM0295  AC34 HEK-293T C287(1.07)  LDD1534  [4]
 LDCM0296  AC35 HEK-293T C31(1.05)  LDD1535  [4]
 LDCM0299  AC38 HEK-293T C340(0.82); C287(0.92)  LDD1538  [4]
 LDCM0300  AC39 HEK-293T C287(1.24)  LDD1539  [4]
 LDCM0302  AC40 HEK-293T C340(1.14)  LDD1541  [4]
 LDCM0304  AC42 HEK-293T C287(0.93)  LDD1543  [4]
 LDCM0305  AC43 HEK-293T C31(0.93)  LDD1544  [4]
 LDCM0308  AC46 HEK-293T C340(0.81); C287(0.88)  LDD1547  [4]
 LDCM0309  AC47 HEK-293T C287(1.27)  LDD1548  [4]
 LDCM0310  AC48 HEK-293T C340(1.11)  LDD1549  [4]
 LDCM0313  AC50 HEK-293T C287(1.10)  LDD1552  [4]
 LDCM0314  AC51 HEK-293T C31(0.98)  LDD1553  [4]
 LDCM0317  AC54 HEK-293T C340(0.82); C287(0.98)  LDD1556  [4]
 LDCM0318  AC55 HEK-293T C287(1.04)  LDD1557  [4]
 LDCM0319  AC56 HEK-293T C340(1.06)  LDD1558  [4]
 LDCM0321  AC58 HEK-293T C287(0.97)  LDD1560  [4]
 LDCM0322  AC59 HEK-293T C31(1.27)  LDD1561  [4]
 LDCM0323  AC6 HEK-293T C340(0.94); C287(0.94)  LDD1562  [4]
 LDCM0326  AC62 HEK-293T C340(0.80); C287(0.92)  LDD1565  [4]
 LDCM0327  AC63 HEK-293T C287(1.24)  LDD1566  [4]
 LDCM0328  AC64 HEK-293T C340(1.00)  LDD1567  [4]
 LDCM0334  AC7 HEK-293T C287(1.08)  LDD1568  [4]
 LDCM0345  AC8 HEK-293T C340(1.11)  LDD1569  [4]
 LDCM0275  AKOS034007705 HEK-293T C340(0.99)  LDD1514  [4]
 LDCM0368  CL10 HEK-293T C340(0.95); C287(1.17)  LDD1572  [4]
 LDCM0371  CL102 HEK-293T C340(1.09)  LDD1575  [4]
 LDCM0375  CL106 HEK-293T C340(1.00)  LDD1579  [4]
 LDCM0379  CL11 HEK-293T C287(1.13)  LDD1583  [4]
 LDCM0380  CL110 HEK-293T C340(1.09)  LDD1584  [4]
 LDCM0384  CL114 HEK-293T C340(0.93)  LDD1588  [4]
 LDCM0388  CL118 HEK-293T C340(0.96)  LDD1592  [4]
 LDCM0390  CL12 HEK-293T C340(1.05)  LDD1594  [4]
 LDCM0393  CL122 HEK-293T C340(0.92)  LDD1597  [4]
 LDCM0397  CL126 HEK-293T C340(1.09)  LDD1601  [4]
 LDCM0401  CL14 HEK-293T C340(1.16)  LDD1605  [4]
 LDCM0405  CL18 HEK-293T C287(1.21)  LDD1609  [4]
 LDCM0406  CL19 HEK-293T C31(1.16)  LDD1610  [4]
 LDCM0407  CL2 HEK-293T C340(1.09)  LDD1611  [4]
 LDCM0410  CL22 HEK-293T C340(0.81); C287(1.00)  LDD1614  [4]
 LDCM0411  CL23 HEK-293T C287(0.99)  LDD1615  [4]
 LDCM0412  CL24 HEK-293T C340(1.03)  LDD1616  [4]
 LDCM0414  CL26 HEK-293T C340(1.02)  LDD1618  [4]
 LDCM0419  CL30 HEK-293T C287(0.84)  LDD1623  [4]
 LDCM0420  CL31 HEK-293T C31(1.02)  LDD1624  [4]
 LDCM0423  CL34 HEK-293T C340(0.89); C287(1.33)  LDD1627  [4]
 LDCM0424  CL35 HEK-293T C287(1.51)  LDD1628  [4]
 LDCM0425  CL36 HEK-293T C340(1.13)  LDD1629  [4]
 LDCM0432  CL42 HEK-293T C287(0.97)  LDD1636  [4]
 LDCM0433  CL43 HEK-293T C31(0.97)  LDD1637  [4]
 LDCM0436  CL46 HEK-293T C340(1.00); C287(1.01)  LDD1640  [4]
 LDCM0437  CL47 HEK-293T C287(1.12)  LDD1641  [4]
 LDCM0438  CL48 HEK-293T C340(1.12)  LDD1642  [4]
 LDCM0441  CL50 HEK-293T C340(0.98)  LDD1645  [4]
 LDCM0445  CL54 HEK-293T C287(0.92)  LDD1648  [4]
 LDCM0446  CL55 HEK-293T C31(1.20)  LDD1649  [4]
 LDCM0449  CL58 HEK-293T C340(0.82); C287(0.94)  LDD1652  [4]
 LDCM0450  CL59 HEK-293T C287(1.17)  LDD1653  [4]
 LDCM0451  CL6 HEK-293T C287(1.04)  LDD1654  [4]
 LDCM0452  CL60 HEK-293T C340(1.19)  LDD1655  [4]
 LDCM0454  CL62 HEK-293T C340(0.93)  LDD1657  [4]
 LDCM0458  CL66 HEK-293T C287(1.02)  LDD1661  [4]
 LDCM0459  CL67 HEK-293T C31(1.11)  LDD1662  [4]
 LDCM0462  CL7 HEK-293T C31(1.31)  LDD1665  [4]
 LDCM0463  CL70 HEK-293T C340(0.95); C287(0.84)  LDD1666  [4]
 LDCM0464  CL71 HEK-293T C287(1.25)  LDD1667  [4]
 LDCM0465  CL72 HEK-293T C340(1.12)  LDD1668  [4]
 LDCM0467  CL74 HEK-293T C340(1.03)  LDD1670  [4]
 LDCM0471  CL78 HEK-293T C287(0.88)  LDD1674  [4]
 LDCM0472  CL79 HEK-293T C31(1.09)  LDD1675  [4]
 LDCM0476  CL82 HEK-293T C340(0.94); C287(0.82)  LDD1679  [4]
 LDCM0477  CL83 HEK-293T C287(1.23)  LDD1680  [4]
 LDCM0478  CL84 HEK-293T C340(1.08)  LDD1681  [4]
 LDCM0480  CL86 HEK-293T C340(0.97)  LDD1683  [4]
 LDCM0485  CL90 HEK-293T C287(1.10)  LDD1688  [4]
 LDCM0486  CL91 HEK-293T C31(1.27)  LDD1689  [4]
 LDCM0489  CL94 HEK-293T C340(0.90); C287(1.03)  LDD1692  [4]
 LDCM0490  CL95 HEK-293T C287(0.98)  LDD1693  [4]
 LDCM0491  CL96 HEK-293T C340(1.13)  LDD1694  [4]
 LDCM0493  CL98 HEK-293T C340(0.99)  LDD1696  [4]
 LDCM0427  Fragment51 HEK-293T C340(0.94)  LDD1631  [4]
 LDCM0022  KB02 HEK-293T C287(1.02)  LDD1492  [4]
 LDCM0023  KB03 Jurkat C478(23.38)  LDD0209  [2]
 LDCM0024  KB05 NB1 C340(2.14)  LDD3340  [5]

References

1 From chemoproteomic-detected amino acids to genomic coordinates: insights into precise multi-omic data integration. Mol Syst Biol. 2021 Feb;17(2):e9840. doi: 10.15252/msb.20209840.
Mass spectrometry data entry: PXD022151
2 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
3 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840