Details of the Target

General Information of Target

Target ID LDTP11181
Target Name Alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrial (ALKBH7)
Gene Name ALKBH7
Gene ID 84266
Synonyms
ABH7; SPATA11; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrial; EC 1.14.11.-; Alkylated DNA repair protein alkB homolog 7; Spermatogenesis cell proliferation-related protein; Spermatogenesis-associated protein 11
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MADSSGRGAGKPATGPTNSSSAKKKDKRVQGGRVIESRYLQYEKKTTQKAPAGDGSQTRG
KMSEGGRKSSLLQKSKADSSGVGKGDLQSTLLEGHGTAPPDLDLSAINDKSIVKKTPQLA
KTISKKPESTSFSAPRKKSPDLSEAMEMMESQTLLLTLLSVKMENNLAEFERRAEKNLLI
MCKEKEKLQKKAHELKRRLLLSQRKRELADVLDAQIEMLSPFEAVATRFKEQYRTFATAL
DTTRHELPVRSIHLEGDGQQLLDALQHELVTTQRLLGELDVGDSEENVQVLDLLSELKDV
TAKKDLELRRSFAQVLELSAEASKEAALANQEVWEETQGMAPPSRWYFNQDSACRESGGA
PKNTPLSEDDNPGASSAPAQATFISPSEDFSSSSQAEVPPSLSRSGRDLS
Target Bioclass
Enzyme
Family
AlkB family
Subcellular location
Mitochondrion matrix
Function
May function as protein hydroxylase; can catalyze auto-hydroxylation at Leu-110 (in vitro), but this activity may be due to the absence of the true substrate. Required to induce programmed necrosis in response to DNA damage caused by cytotoxic alkylating agents. Acts by triggering the collapse of mitochondrial membrane potential and loss of mitochondrial function that leads to energy depletion and cell death. ALKBH7-mediated necrosis is probably required to prevent the accumulation of cells with DNA damage. Does not display DNA demethylase activity. Involved in fatty acid metabolism.
Uniprot ID
Q9BT30
Ensemble ID
ENST00000245812.8
HGNC ID
HGNC:21306

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C128(1.25)  LDD1094  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0166  [2]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [3]
TFBX
 Probe Info  		N.A.  LDD0148  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 PaTu 8988t C128(1.25)  LDD1094  [1]
 LDCM0226  AC11 PaTu 8988t C128(1.19)  LDD1105  [1]
 LDCM0237  AC12 PaTu 8988t C128(1.16)  LDD1116  [1]
 LDCM0259  AC14 PaTu 8988t C128(1.11)  LDD1138  [1]
 LDCM0270  AC15 PaTu 8988t C128(1.17)  LDD1149  [1]
 LDCM0276  AC17 PaTu 8988t C128(1.03)  LDD1155  [1]
 LDCM0277  AC18 PaTu 8988t C128(0.80)  LDD1156  [1]
 LDCM0278  AC19 PaTu 8988t C128(0.90)  LDD1157  [1]
 LDCM0280  AC20 PaTu 8988t C128(0.76)  LDD1159  [1]
 LDCM0281  AC21 PaTu 8988t C128(0.65)  LDD1160  [1]
 LDCM0282  AC22 PaTu 8988t C128(0.78)  LDD1161  [1]
 LDCM0283  AC23 PaTu 8988t C128(0.85)  LDD1162  [1]
 LDCM0284  AC24 PaTu 8988t C128(0.89)  LDD1163  [1]
 LDCM0292  AC31 HEK-293T C128(1.01)  LDD1531  [5]
 LDCM0296  AC35 PaTu 8988t C128(0.94)  LDD1175  [1]
 LDCM0297  AC36 PaTu 8988t C128(1.06)  LDD1176  [1]
 LDCM0298  AC37 PaTu 8988t C128(1.08)  LDD1177  [1]
 LDCM0299  AC38 PaTu 8988t C128(1.26)  LDD1178  [1]
 LDCM0300  AC39 PaTu 8988t C128(1.17)  LDD1179  [1]
 LDCM0302  AC40 PaTu 8988t C128(1.04)  LDD1181  [1]
 LDCM0303  AC41 PaTu 8988t C128(1.02)  LDD1182  [1]
 LDCM0304  AC42 PaTu 8988t C128(0.98)  LDD1183  [1]
 LDCM0305  AC43 PaTu 8988t C128(1.13)  LDD1184  [1]
 LDCM0306  AC44 PaTu 8988t C128(1.10)  LDD1185  [1]
 LDCM0307  AC45 PaTu 8988t C128(1.10)  LDD1186  [1]
 LDCM0308  AC46 PaTu 8988t C128(0.88)  LDD1187  [1]
 LDCM0309  AC47 PaTu 8988t C128(0.89)  LDD1188  [1]
 LDCM0310  AC48 PaTu 8988t C128(0.97)  LDD1189  [1]
 LDCM0311  AC49 PaTu 8988t C128(0.83)  LDD1190  [1]
 LDCM0313  AC50 PaTu 8988t C128(0.79)  LDD1192  [1]
 LDCM0314  AC51 PaTu 8988t C128(1.07)  LDD1193  [1]
 LDCM0315  AC52 PaTu 8988t C128(0.84)  LDD1194  [1]
 LDCM0316  AC53 PaTu 8988t C128(0.71)  LDD1195  [1]
 LDCM0317  AC54 PaTu 8988t C128(0.79)  LDD1196  [1]
 LDCM0318  AC55 PaTu 8988t C128(1.01)  LDD1197  [1]
 LDCM0319  AC56 PaTu 8988t C128(0.86)  LDD1198  [1]
 LDCM0320  AC57 PaTu 8988t C128(0.82)  LDD1199  [1]
 LDCM0321  AC58 PaTu 8988t C128(0.91)  LDD1200  [1]
 LDCM0322  AC59 PaTu 8988t C128(0.96)  LDD1201  [1]
 LDCM0323  AC6 PaTu 8988t C128(1.17)  LDD1202  [1]
 LDCM0324  AC60 PaTu 8988t C128(1.19)  LDD1203  [1]
 LDCM0325  AC61 PaTu 8988t C128(1.07)  LDD1204  [1]
 LDCM0326  AC62 PaTu 8988t C128(0.82)  LDD1205  [1]
 LDCM0327  AC63 PaTu 8988t C128(0.85)  LDD1206  [1]
 LDCM0328  AC64 PaTu 8988t C128(0.98)  LDD1207  [1]
 LDCM0329  AC65 PaTu 8988t C128(0.62)  LDD1208  [1]
 LDCM0330  AC66 PaTu 8988t C128(0.83)  LDD1209  [1]
 LDCM0331  AC67 PaTu 8988t C128(0.76)  LDD1210  [1]
 LDCM0334  AC7 PaTu 8988t C128(1.58)  LDD1213  [1]
 LDCM0345  AC8 PaTu 8988t C128(1.17)  LDD1224  [1]
 LDCM0248  AKOS034007472 PaTu 8988t C128(1.12)  LDD1127  [1]
 LDCM0356  AKOS034007680 PaTu 8988t C128(1.37)  LDD1235  [1]
 LDCM0275  AKOS034007705 PaTu 8988t C128(1.21)  LDD1154  [1]
 LDCM0367  CL1 HEK-293T C128(1.04)  LDD1571  [5]
 LDCM0369  CL100 HEK-293T C128(0.93)  LDD1573  [5]
 LDCM0370  CL101 PaTu 8988t C128(1.19)  LDD1249  [1]
 LDCM0371  CL102 PaTu 8988t C128(1.61)  LDD1250  [1]
 LDCM0372  CL103 PaTu 8988t C128(1.19)  LDD1251  [1]
 LDCM0373  CL104 PaTu 8988t C128(1.54)  LDD1252  [1]
 LDCM0374  CL105 PaTu 8988t C128(1.03)  LDD1253  [1]
 LDCM0375  CL106 PaTu 8988t C128(0.84)  LDD1254  [1]
 LDCM0376  CL107 PaTu 8988t C128(1.02)  LDD1255  [1]
 LDCM0377  CL108 PaTu 8988t C128(0.69)  LDD1256  [1]
 LDCM0378  CL109 PaTu 8988t C128(0.96)  LDD1257  [1]
 LDCM0379  CL11 HEK-293T C128(1.26)  LDD1583  [5]
 LDCM0380  CL110 PaTu 8988t C128(1.05)  LDD1259  [1]
 LDCM0381  CL111 PaTu 8988t C128(0.80)  LDD1260  [1]
 LDCM0382  CL112 HEK-293T C128(0.95)  LDD1586  [5]
 LDCM0383  CL113 HEK-293T C128(0.99)  LDD1587  [5]
 LDCM0385  CL115 HEK-293T C128(1.16)  LDD1589  [5]
 LDCM0386  CL116 HEK-293T C128(1.04)  LDD1590  [5]
 LDCM0387  CL117 PaTu 8988t C128(1.00)  LDD1266  [1]
 LDCM0388  CL118 PaTu 8988t C128(0.88)  LDD1267  [1]
 LDCM0389  CL119 PaTu 8988t C128(0.93)  LDD1268  [1]
 LDCM0391  CL120 PaTu 8988t C128(1.18)  LDD1270  [1]
 LDCM0392  CL121 PaTu 8988t C128(1.00)  LDD1271  [1]
 LDCM0393  CL122 PaTu 8988t C128(0.81)  LDD1272  [1]
 LDCM0394  CL123 PaTu 8988t C128(1.11)  LDD1273  [1]
 LDCM0395  CL124 PaTu 8988t C128(1.13)  LDD1274  [1]
 LDCM0396  CL125 PaTu 8988t C128(0.70)  LDD1275  [1]
 LDCM0397  CL126 PaTu 8988t C128(0.95)  LDD1276  [1]
 LDCM0398  CL127 PaTu 8988t C128(0.69)  LDD1277  [1]
 LDCM0399  CL128 PaTu 8988t C128(0.90)  LDD1278  [1]
 LDCM0400  CL13 HEK-293T C128(1.11)  LDD1604  [5]
 LDCM0402  CL15 HEK-293T C128(1.05)  LDD1606  [5]
 LDCM0403  CL16 HEK-293T C128(1.19)  LDD1607  [5]
 LDCM0411  CL23 HEK-293T C128(1.03)  LDD1615  [5]
 LDCM0413  CL25 HEK-293T C128(1.18)  LDD1617  [5]
 LDCM0415  CL27 HEK-293T C128(0.80)  LDD1619  [5]
 LDCM0416  CL28 HEK-293T C128(1.02)  LDD1620  [5]
 LDCM0418  CL3 HEK-293T C128(0.79)  LDD1622  [5]
 LDCM0424  CL35 HEK-293T C128(0.91)  LDD1628  [5]
 LDCM0426  CL37 HEK-293T C128(1.21)  LDD1630  [5]
 LDCM0428  CL39 HEK-293T C128(0.95)  LDD1632  [5]
 LDCM0429  CL4 HEK-293T C128(1.29)  LDD1633  [5]
 LDCM0430  CL40 HEK-293T C128(1.21)  LDD1634  [5]
 LDCM0437  CL47 HEK-293T C128(1.41)  LDD1641  [5]
 LDCM0439  CL49 HEK-293T C128(0.95)  LDD1643  [5]
 LDCM0443  CL52 HEK-293T C128(1.33)  LDD1646  [5]
 LDCM0450  CL59 HEK-293T C128(1.19)  LDD1653  [5]
 LDCM0453  CL61 HEK-293T C128(0.95)  LDD1656  [5]
 LDCM0455  CL63 HEK-293T C128(0.89)  LDD1658  [5]
 LDCM0456  CL64 HEK-293T C128(0.95)  LDD1659  [5]
 LDCM0464  CL71 HEK-293T C128(1.15)  LDD1667  [5]
 LDCM0466  CL73 HEK-293T C128(1.61)  LDD1669  [5]
 LDCM0469  CL76 HEK-293T C128(0.98)  LDD1672  [5]
 LDCM0477  CL83 HEK-293T C128(1.03)  LDD1680  [5]
 LDCM0479  CL85 HEK-293T C128(1.16)  LDD1682  [5]
 LDCM0481  CL87 HEK-293T C128(1.05)  LDD1684  [5]
 LDCM0482  CL88 HEK-293T C128(1.15)  LDD1685  [5]
 LDCM0490  CL95 HEK-293T C128(1.04)  LDD1693  [5]
 LDCM0492  CL97 HEK-293T C128(1.01)  LDD1695  [5]
 LDCM0494  CL99 HEK-293T C128(0.91)  LDD1697  [5]
 LDCM0495  E2913 HEK-293T C128(0.97)  LDD1698  [5]
 LDCM0468  Fragment33 HEK-293T C128(1.02)  LDD1671  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
B9 domain-containing protein 2 (B9D2) B9D family Q9BPU9
Ciliary microtubule inner protein 1 (CIMIP1) . Q9H1P6

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402