Details of the Target

General Information of Target

Target ID LDTP10428
Target Name Putative protein-lysine deacylase ABHD14B (ABHD14B)
Gene Name ABHD14B
Gene ID 84836
Synonyms
CIB; Putative protein-lysine deacylase ABHD14B; EC 2.3.1.-; Alpha/beta hydrolase domain-containing protein 14B; Abhydrolase domain-containing protein 14B; CCG1-interacting factor B
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRSGEPACTMDQARGLDDAAARGGQCPGLGPAPTPTPPGRLGAPYSEAWGYFHLAPGRPG
HPSGHWATCRLCGEQVGRGPGFHAGTSALWRHLRSAHRRELESSGAGSSPPAAPCPPPPG
PAAAPEGDWARLLEQMGALAVRGSRRERELERRELAVEQGERALERRRRALQEEERAAAQ
ARRELQAEREALQARLRDVSRREGALGWAPAAPPPLKDDPEGDRDGCVITKVLL
Target Bioclass
Enzyme
Family
AB hydrolase superfamily, ABHD14 family
Subcellular location
Cytoplasm
Function
Acts as an atypical protein-lysine deacetylase in vitro. Catalyzes the deacetylation of lysine residues using CoA as substrate, generating acetyl-CoA and the free amine of protein-lysine residues. Additional experiments are however required to confirm the protein-lysine deacetylase activity in vivo (Probable). Has hydrolase activity towards various surrogate p-nitrophenyl (pNp) substrates, such as pNp-butyrate, pNp-acetate and pNp-octanoate in vitro, with a strong preference for pNp-acetate. May activate transcription.
Uniprot ID
Q96IU4
Ensemble ID
ENST00000361143.10
HGNC ID
HGNC:28235

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 14 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TH211
 Probe Info  		Y158(12.34)  LDD0257  [1]
STPyne
 Probe Info  		K173(13.98)  LDD2217  [2]
Probe 1
 Probe Info  		Y147(89.68)  LDD3495  [3]
DBIA
 Probe Info  		C190(1.22)  LDD3332  [4]
Acrolein
 Probe Info  		H171(0.00); H39(0.00); H55(0.00); H74(0.00)  LDD0223  [5]
5E-2FA
 Probe Info  		H74(0.00); H171(0.00)  LDD2235  [6]
m-APA
 Probe Info  		H74(0.00); H171(0.00); H55(0.00)  LDD2231  [6]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [7]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [7]
IPIAA_H
 Probe Info  		N.A.  LDD0030  [8]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [8]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [7]
IPM
 Probe Info  		C190(0.00); C138(0.00)  LDD2156  [9]
NAIA_5
 Probe Info  		N.A.  LDD2223  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C190(0.93)  LDD1507  [11]
 LDCM0215  AC10 HEK-293T C190(0.96)  LDD1508  [11]
 LDCM0226  AC11 HEK-293T C190(1.03)  LDD1509  [11]
 LDCM0237  AC12 HEK-293T C190(0.91)  LDD1510  [11]
 LDCM0259  AC14 HEK-293T C190(1.01)  LDD1512  [11]
 LDCM0270  AC15 HEK-293T C190(0.93)  LDD1513  [11]
 LDCM0276  AC17 HEK-293T C190(1.05)  LDD1515  [11]
 LDCM0277  AC18 HEK-293T C190(0.95)  LDD1516  [11]
 LDCM0278  AC19 HEK-293T C190(0.84)  LDD1517  [11]
 LDCM0279  AC2 HEK-293T C190(1.10)  LDD1518  [11]
 LDCM0280  AC20 HEK-293T C190(0.96)  LDD1519  [11]
 LDCM0281  AC21 HEK-293T C190(0.98)  LDD1520  [11]
 LDCM0282  AC22 HEK-293T C190(1.00)  LDD1521  [11]
 LDCM0283  AC23 HEK-293T C190(0.84)  LDD1522  [11]
 LDCM0285  AC25 HEK-293T C190(0.92)  LDD1524  [11]
 LDCM0286  AC26 HEK-293T C190(1.08)  LDD1525  [11]
 LDCM0287  AC27 HEK-293T C190(0.97)  LDD1526  [11]
 LDCM0288  AC28 HEK-293T C190(0.98)  LDD1527  [11]
 LDCM0289  AC29 HEK-293T C190(1.09)  LDD1528  [11]
 LDCM0290  AC3 HEK-293T C190(1.01)  LDD1529  [11]
 LDCM0291  AC30 HEK-293T C190(0.91)  LDD1530  [11]
 LDCM0292  AC31 HEK-293T C190(1.00)  LDD1531  [11]
 LDCM0294  AC33 HEK-293T C190(0.84)  LDD1533  [11]
 LDCM0295  AC34 HEK-293T C190(1.04)  LDD1534  [11]
 LDCM0296  AC35 HEK-293T C190(0.98)  LDD1535  [11]
 LDCM0297  AC36 HEK-293T C190(1.06)  LDD1536  [11]
 LDCM0298  AC37 HEK-293T C190(1.04)  LDD1537  [11]
 LDCM0299  AC38 HEK-293T C190(0.91)  LDD1538  [11]
 LDCM0300  AC39 HEK-293T C190(0.87)  LDD1539  [11]
 LDCM0301  AC4 HEK-293T C190(0.92)  LDD1540  [11]
 LDCM0303  AC41 HEK-293T C190(0.87)  LDD1542  [11]
 LDCM0304  AC42 HEK-293T C190(1.14)  LDD1543  [11]
 LDCM0305  AC43 HEK-293T C190(0.95)  LDD1544  [11]
 LDCM0306  AC44 HEK-293T C190(0.92)  LDD1545  [11]
 LDCM0307  AC45 HEK-293T C190(1.03)  LDD1546  [11]
 LDCM0308  AC46 HEK-293T C190(0.95)  LDD1547  [11]
 LDCM0309  AC47 HEK-293T C190(1.01)  LDD1548  [11]
 LDCM0311  AC49 HEK-293T C190(0.89)  LDD1550  [11]
 LDCM0312  AC5 HEK-293T C190(1.05)  LDD1551  [11]
 LDCM0313  AC50 HEK-293T C190(1.07)  LDD1552  [11]
 LDCM0314  AC51 HEK-293T C190(1.03)  LDD1553  [11]
 LDCM0315  AC52 HEK-293T C190(0.95)  LDD1554  [11]
 LDCM0316  AC53 HEK-293T C190(1.07)  LDD1555  [11]
 LDCM0317  AC54 HEK-293T C190(1.05)  LDD1556  [11]
 LDCM0318  AC55 HEK-293T C190(0.86)  LDD1557  [11]
 LDCM0320  AC57 HEK-293T C190(0.88)  LDD1559  [11]
 LDCM0321  AC58 HEK-293T C190(1.02)  LDD1560  [11]
 LDCM0322  AC59 HEK-293T C190(0.96)  LDD1561  [11]
 LDCM0323  AC6 HEK-293T C190(1.00)  LDD1562  [11]
 LDCM0324  AC60 HEK-293T C190(0.95)  LDD1563  [11]
 LDCM0325  AC61 HEK-293T C190(0.96)  LDD1564  [11]
 LDCM0326  AC62 HEK-293T C190(0.87)  LDD1565  [11]
 LDCM0327  AC63 HEK-293T C190(0.82)  LDD1566  [11]
 LDCM0334  AC7 HEK-293T C190(0.95)  LDD1568  [11]
 LDCM0248  AKOS034007472 HEK-293T C190(1.00)  LDD1511  [11]
 LDCM0356  AKOS034007680 HEK-293T C190(0.88)  LDD1570  [11]
 LDCM0367  CL1 HEK-293T C190(1.07)  LDD1571  [11]
 LDCM0368  CL10 HEK-293T C190(0.78)  LDD1572  [11]
 LDCM0369  CL100 HEK-293T C190(0.97)  LDD1573  [11]
 LDCM0370  CL101 HEK-293T C190(1.09)  LDD1574  [11]
 LDCM0371  CL102 HEK-293T C190(0.92)  LDD1575  [11]
 LDCM0372  CL103 HEK-293T C190(0.95)  LDD1576  [11]
 LDCM0373  CL104 HEK-293T C190(1.02)  LDD1577  [11]
 LDCM0374  CL105 HEK-293T C190(1.04)  LDD1578  [11]
 LDCM0375  CL106 HEK-293T C190(0.95)  LDD1579  [11]
 LDCM0376  CL107 HEK-293T C190(0.95)  LDD1580  [11]
 LDCM0377  CL108 HEK-293T C190(0.96)  LDD1581  [11]
 LDCM0378  CL109 HEK-293T C190(0.94)  LDD1582  [11]
 LDCM0379  CL11 HEK-293T C190(1.00)  LDD1583  [11]
 LDCM0380  CL110 HEK-293T C190(0.87)  LDD1584  [11]
 LDCM0381  CL111 HEK-293T C190(1.03)  LDD1585  [11]
 LDCM0382  CL112 HEK-293T C190(1.03)  LDD1586  [11]
 LDCM0383  CL113 HEK-293T C190(0.96)  LDD1587  [11]
 LDCM0384  CL114 HEK-293T C190(1.02)  LDD1588  [11]
 LDCM0385  CL115 HEK-293T C190(1.05)  LDD1589  [11]
 LDCM0386  CL116 HEK-293T C190(1.02)  LDD1590  [11]
 LDCM0387  CL117 HEK-293T C190(0.81)  LDD1591  [11]
 LDCM0388  CL118 HEK-293T C190(0.90)  LDD1592  [11]
 LDCM0389  CL119 HEK-293T C190(0.95)  LDD1593  [11]
 LDCM0391  CL120 HEK-293T C190(1.11)  LDD1595  [11]
 LDCM0392  CL121 HEK-293T C190(1.04)  LDD1596  [11]
 LDCM0393  CL122 HEK-293T C190(0.89)  LDD1597  [11]
 LDCM0394  CL123 HEK-293T C190(0.98)  LDD1598  [11]
 LDCM0395  CL124 HEK-293T C190(0.96)  LDD1599  [11]
 LDCM0396  CL125 HEK-293T C190(1.09)  LDD1600  [11]
 LDCM0397  CL126 HEK-293T C190(0.92)  LDD1601  [11]
 LDCM0398  CL127 HEK-293T C190(0.90)  LDD1602  [11]
 LDCM0399  CL128 HEK-293T C190(0.95)  LDD1603  [11]
 LDCM0400  CL13 HEK-293T C190(0.94)  LDD1604  [11]
 LDCM0401  CL14 HEK-293T C190(0.85)  LDD1605  [11]
 LDCM0402  CL15 HEK-293T C190(0.82)  LDD1606  [11]
 LDCM0403  CL16 HEK-293T C190(0.96)  LDD1607  [11]
 LDCM0404  CL17 HEK-293T C190(0.83)  LDD1608  [11]
 LDCM0405  CL18 HEK-293T C190(1.11)  LDD1609  [11]
 LDCM0406  CL19 HEK-293T C190(0.95)  LDD1610  [11]
 LDCM0407  CL2 HEK-293T C190(0.96)  LDD1611  [11]
 LDCM0408  CL20 HEK-293T C190(0.98)  LDD1612  [11]
 LDCM0409  CL21 HEK-293T C190(0.90)  LDD1613  [11]
 LDCM0410  CL22 HEK-293T C190(0.95)  LDD1614  [11]
 LDCM0411  CL23 HEK-293T C190(0.95)  LDD1615  [11]
 LDCM0413  CL25 HEK-293T C190(1.08)  LDD1617  [11]
 LDCM0414  CL26 HEK-293T C190(0.87)  LDD1618  [11]
 LDCM0415  CL27 HEK-293T C190(1.05)  LDD1619  [11]
 LDCM0416  CL28 HEK-293T C190(1.03)  LDD1620  [11]
 LDCM0417  CL29 HEK-293T C190(0.86)  LDD1621  [11]
 LDCM0418  CL3 HEK-293T C190(1.04)  LDD1622  [11]
 LDCM0419  CL30 HEK-293T C190(1.05)  LDD1623  [11]
 LDCM0420  CL31 HEK-293T C190(1.01)  LDD1624  [11]
 LDCM0421  CL32 HEK-293T C190(1.09)  LDD1625  [11]
 LDCM0422  CL33 HEK-293T C190(1.05)  LDD1626  [11]
 LDCM0423  CL34 HEK-293T C190(1.02)  LDD1627  [11]
 LDCM0424  CL35 HEK-293T C190(0.93)  LDD1628  [11]
 LDCM0426  CL37 HEK-293T C190(1.03)  LDD1630  [11]
 LDCM0428  CL39 HEK-293T C190(0.99)  LDD1632  [11]
 LDCM0429  CL4 HEK-293T C190(0.99)  LDD1633  [11]
 LDCM0430  CL40 HEK-293T C190(0.96)  LDD1634  [11]
 LDCM0431  CL41 HEK-293T C190(0.81)  LDD1635  [11]
 LDCM0432  CL42 HEK-293T C190(1.09)  LDD1636  [11]
 LDCM0433  CL43 HEK-293T C190(0.83)  LDD1637  [11]
 LDCM0434  CL44 HEK-293T C190(0.82)  LDD1638  [11]
 LDCM0435  CL45 HEK-293T C190(1.01)  LDD1639  [11]
 LDCM0436  CL46 HEK-293T C190(0.98)  LDD1640  [11]
 LDCM0437  CL47 HEK-293T C190(0.84)  LDD1641  [11]
 LDCM0439  CL49 HEK-293T C190(0.89)  LDD1643  [11]
 LDCM0440  CL5 HEK-293T C190(0.99)  LDD1644  [11]
 LDCM0441  CL50 HEK-293T C190(0.90)  LDD1645  [11]
 LDCM0443  CL52 HEK-293T C190(0.99)  LDD1646  [11]
 LDCM0444  CL53 HEK-293T C190(0.97)  LDD1647  [11]
 LDCM0445  CL54 HEK-293T C190(1.15)  LDD1648  [11]
 LDCM0446  CL55 HEK-293T C190(1.00)  LDD1649  [11]
 LDCM0447  CL56 HEK-293T C190(0.99)  LDD1650  [11]
 LDCM0448  CL57 HEK-293T C190(1.11)  LDD1651  [11]
 LDCM0449  CL58 HEK-293T C190(0.91)  LDD1652  [11]
 LDCM0450  CL59 HEK-293T C190(0.86)  LDD1653  [11]
 LDCM0451  CL6 HEK-293T C190(1.06)  LDD1654  [11]
 LDCM0453  CL61 HEK-293T C190(0.94)  LDD1656  [11]
 LDCM0454  CL62 HEK-293T C190(0.85)  LDD1657  [11]
 LDCM0455  CL63 HEK-293T C190(0.95)  LDD1658  [11]
 LDCM0456  CL64 HEK-293T C190(1.05)  LDD1659  [11]
 LDCM0457  CL65 HEK-293T C190(0.95)  LDD1660  [11]
 LDCM0458  CL66 HEK-293T C190(1.08)  LDD1661  [11]
 LDCM0459  CL67 HEK-293T C190(0.94)  LDD1662  [11]
 LDCM0460  CL68 HEK-293T C190(1.15)  LDD1663  [11]
 LDCM0461  CL69 HEK-293T C190(1.06)  LDD1664  [11]
 LDCM0462  CL7 HEK-293T C190(1.01)  LDD1665  [11]
 LDCM0463  CL70 HEK-293T C190(0.91)  LDD1666  [11]
 LDCM0464  CL71 HEK-293T C190(0.93)  LDD1667  [11]
 LDCM0466  CL73 HEK-293T C190(0.86)  LDD1669  [11]
 LDCM0467  CL74 HEK-293T C190(0.90)  LDD1670  [11]
 LDCM0469  CL76 HEK-293T C190(1.02)  LDD1672  [11]
 LDCM0470  CL77 HEK-293T C190(0.91)  LDD1673  [11]
 LDCM0471  CL78 HEK-293T C190(1.15)  LDD1674  [11]
 LDCM0472  CL79 HEK-293T C190(1.12)  LDD1675  [11]
 LDCM0473  CL8 HEK-293T C190(0.77)  LDD1676  [11]
 LDCM0474  CL80 HEK-293T C190(1.00)  LDD1677  [11]
 LDCM0475  CL81 HEK-293T C190(0.93)  LDD1678  [11]
 LDCM0476  CL82 HEK-293T C190(1.05)  LDD1679  [11]
 LDCM0477  CL83 HEK-293T C190(1.00)  LDD1680  [11]
 LDCM0479  CL85 HEK-293T C190(0.88)  LDD1682  [11]
 LDCM0480  CL86 HEK-293T C190(0.89)  LDD1683  [11]
 LDCM0481  CL87 HEK-293T C190(0.89)  LDD1684  [11]
 LDCM0482  CL88 HEK-293T C190(1.02)  LDD1685  [11]
 LDCM0483  CL89 HEK-293T C190(0.93)  LDD1686  [11]
 LDCM0484  CL9 HEK-293T C190(1.08)  LDD1687  [11]
 LDCM0485  CL90 HEK-293T C190(1.03)  LDD1688  [11]
 LDCM0486  CL91 HEK-293T C190(1.04)  LDD1689  [11]
 LDCM0487  CL92 HEK-293T C190(0.96)  LDD1690  [11]
 LDCM0488  CL93 HEK-293T C190(0.96)  LDD1691  [11]
 LDCM0489  CL94 HEK-293T C190(0.90)  LDD1692  [11]
 LDCM0490  CL95 HEK-293T C190(0.83)  LDD1693  [11]
 LDCM0492  CL97 HEK-293T C190(1.04)  LDD1695  [11]
 LDCM0493  CL98 HEK-293T C190(0.93)  LDD1696  [11]
 LDCM0494  CL99 HEK-293T C190(0.98)  LDD1697  [11]
 LDCM0495  E2913 HEK-293T C190(0.89)  LDD1698  [11]
 LDCM0468  Fragment33 HEK-293T C190(1.01)  LDD1671  [11]
 LDCM0427  Fragment51 HEK-293T C190(0.91)  LDD1631  [11]
 LDCM0022  KB02 HEK-293T C190(0.95)  LDD1492  [11]
 LDCM0023  KB03 HEK-293T C190(0.90)  LDD1497  [11]
 LDCM0024  KB05 MKN45 C190(1.22)  LDD3332  [4]
 LDCM0109  NEM HeLa H171(0.00); H39(0.00); H55(0.00); H74(0.00)  LDD0223  [5]

References

1 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
2 Global profiling of lysine reactivity and ligandability in the human proteome. Nat Chem. 2017 Dec;9(12):1181-1190. doi: 10.1038/nchem.2826. Epub 2017 Jul 31.
3 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
6 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
7 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
8 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
9 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
10 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
11 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402