Details of the Target

General Information of Target

Target ID LDTP10373
Target Name Centromere protein N (CENPN)
Gene Name CENPN
Gene ID 55839
Synonyms
C16orf60; ICEN32; Centromere protein N; CENP-N; Interphase centromere complex protein 32
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MHRRGVGAGAIAKKKLAEAKYKERGTVLAEDQLAQMSKQLDMFKTNLEEFASKHKQEIRK
NPEFRVQFQDMCATIGVDPLASGKGFWSEMLGVGDFYYELGVQIIEVCLALKHRNGGLIT
LEELHQQVLKGRGKFAQDVSQDDLIRAIKKLKALGTGFGIIPVGGTYLIQSVPAELNMDH
TVVLQLAEKNGYVTVSEIKASLKWETERARQVLEHLLKEGLAWLDLQAPGEAHYWLPALF
TDLYSQEITAEEAREALP
Target Bioclass
Other
Family
CENP-N/CHL4 family
Subcellular location
Nucleus
Function
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.
Uniprot ID
Q96H22
Ensemble ID
ENST00000299572.9
HGNC ID
HGNC:30873

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
MEWO SNV: p.R292Ter .
NCIH446 SNV: p.E254G .
RL952 SNV: p.P159L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
NAIA_5
 Probe Info  		C325(0.85)  LDD2227  [1]
DBIA
 Probe Info  		C325(0.98)  LDD1492  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [3]
NAIA_4
 Probe Info  		N.A.  LDD2226  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C56(0.86)  LDD1507  [2]
 LDCM0215  AC10 HEK-293T C325(1.03)  LDD1508  [2]
 LDCM0237  AC12 HEK-293T C325(0.92)  LDD1510  [2]
 LDCM0276  AC17 HEK-293T C56(0.91)  LDD1515  [2]
 LDCM0277  AC18 HEK-293T C325(1.01)  LDD1516  [2]
 LDCM0279  AC2 HEK-293T C325(1.03)  LDD1518  [2]
 LDCM0280  AC20 HEK-293T C325(1.00)  LDD1519  [2]
 LDCM0281  AC21 HEK-293T C325(0.93); C56(0.99)  LDD1520  [2]
 LDCM0284  AC24 HEK-293T C325(0.97)  LDD1523  [2]
 LDCM0285  AC25 HEK-293T C56(0.95)  LDD1524  [2]
 LDCM0286  AC26 HEK-293T C325(0.94)  LDD1525  [2]
 LDCM0288  AC28 HEK-293T C325(1.12)  LDD1527  [2]
 LDCM0289  AC29 HEK-293T C325(1.01); C56(1.05)  LDD1528  [2]
 LDCM0293  AC32 HEK-293T C325(1.00)  LDD1532  [2]
 LDCM0294  AC33 HEK-293T C56(0.96)  LDD1533  [2]
 LDCM0295  AC34 HEK-293T C325(0.94)  LDD1534  [2]
 LDCM0297  AC36 HEK-293T C325(1.01)  LDD1536  [2]
 LDCM0298  AC37 HEK-293T C325(1.09); C56(1.05)  LDD1537  [2]
 LDCM0301  AC4 HEK-293T C325(1.11)  LDD1540  [2]
 LDCM0302  AC40 HEK-293T C325(1.21)  LDD1541  [2]
 LDCM0303  AC41 HEK-293T C56(0.98)  LDD1542  [2]
 LDCM0304  AC42 HEK-293T C325(1.04)  LDD1543  [2]
 LDCM0306  AC44 HEK-293T C325(1.17)  LDD1545  [2]
 LDCM0307  AC45 HEK-293T C325(1.03); C56(0.97)  LDD1546  [2]
 LDCM0310  AC48 HEK-293T C325(1.05)  LDD1549  [2]
 LDCM0311  AC49 HEK-293T C56(0.87)  LDD1550  [2]
 LDCM0312  AC5 HEK-293T C325(0.96); C56(0.97)  LDD1551  [2]
 LDCM0313  AC50 HEK-293T C325(1.06)  LDD1552  [2]
 LDCM0315  AC52 HEK-293T C325(0.95)  LDD1554  [2]
 LDCM0316  AC53 HEK-293T C325(1.03); C56(1.00)  LDD1555  [2]
 LDCM0319  AC56 HEK-293T C325(1.30)  LDD1558  [2]
 LDCM0320  AC57 HEK-293T C56(0.97)  LDD1559  [2]
 LDCM0321  AC58 HEK-293T C325(0.99)  LDD1560  [2]
 LDCM0324  AC60 HEK-293T C325(1.03)  LDD1563  [2]
 LDCM0325  AC61 HEK-293T C325(0.96); C56(0.96)  LDD1564  [2]
 LDCM0328  AC64 HEK-293T C325(1.10)  LDD1567  [2]
 LDCM0345  AC8 HEK-293T C325(1.05)  LDD1569  [2]
 LDCM0248  AKOS034007472 HEK-293T C325(1.00); C56(0.97)  LDD1511  [2]
 LDCM0356  AKOS034007680 HEK-293T C56(0.97)  LDD1570  [2]
 LDCM0275  AKOS034007705 HEK-293T C325(0.99)  LDD1514  [2]
 LDCM0632  CL-Sc Hep-G2 C325(0.85)  LDD2227  [1]
 LDCM0367  CL1 HEK-293T C325(0.94)  LDD1571  [2]
 LDCM0369  CL100 HEK-293T C325(0.99)  LDD1573  [2]
 LDCM0370  CL101 HEK-293T C325(0.87)  LDD1574  [2]
 LDCM0371  CL102 HEK-293T C325(0.91); C56(1.01)  LDD1575  [2]
 LDCM0372  CL103 HEK-293T C325(0.97); C56(0.93)  LDD1576  [2]
 LDCM0373  CL104 HEK-293T C325(0.94)  LDD1577  [2]
 LDCM0374  CL105 HEK-293T C325(0.89)  LDD1578  [2]
 LDCM0375  CL106 HEK-293T C325(0.89); C56(0.97)  LDD1579  [2]
 LDCM0376  CL107 HEK-293T C325(0.89); C56(1.01)  LDD1580  [2]
 LDCM0377  CL108 HEK-293T C325(0.99)  LDD1581  [2]
 LDCM0378  CL109 HEK-293T C325(0.86)  LDD1582  [2]
 LDCM0380  CL110 HEK-293T C325(1.13); C56(0.96)  LDD1584  [2]
 LDCM0381  CL111 HEK-293T C325(0.87); C56(0.95)  LDD1585  [2]
 LDCM0382  CL112 HEK-293T C325(0.89)  LDD1586  [2]
 LDCM0383  CL113 HEK-293T C325(0.85)  LDD1587  [2]
 LDCM0384  CL114 HEK-293T C325(0.78); C56(1.10)  LDD1588  [2]
 LDCM0385  CL115 HEK-293T C325(1.08); C56(0.95)  LDD1589  [2]
 LDCM0386  CL116 HEK-293T C325(1.11)  LDD1590  [2]
 LDCM0387  CL117 HEK-293T C325(0.91)  LDD1591  [2]
 LDCM0388  CL118 HEK-293T C325(1.10); C56(0.97)  LDD1592  [2]
 LDCM0389  CL119 HEK-293T C325(1.12); C56(0.96)  LDD1593  [2]
 LDCM0390  CL12 HEK-293T C325(0.98)  LDD1594  [2]
 LDCM0391  CL120 HEK-293T C325(1.04)  LDD1595  [2]
 LDCM0392  CL121 HEK-293T C325(0.83)  LDD1596  [2]
 LDCM0393  CL122 HEK-293T C325(0.83); C56(0.94)  LDD1597  [2]
 LDCM0394  CL123 HEK-293T C325(0.92); C56(0.96)  LDD1598  [2]
 LDCM0395  CL124 HEK-293T C325(0.88)  LDD1599  [2]
 LDCM0396  CL125 HEK-293T C325(0.96)  LDD1600  [2]
 LDCM0397  CL126 HEK-293T C325(0.52); C56(0.96)  LDD1601  [2]
 LDCM0398  CL127 HEK-293T C325(1.00); C56(0.96)  LDD1602  [2]
 LDCM0399  CL128 HEK-293T C325(1.00)  LDD1603  [2]
 LDCM0400  CL13 HEK-293T C325(0.85)  LDD1604  [2]
 LDCM0401  CL14 HEK-293T C325(0.99); C56(1.06)  LDD1605  [2]
 LDCM0402  CL15 HEK-293T C325(0.85); C56(0.97)  LDD1606  [2]
 LDCM0403  CL16 HEK-293T C325(0.96)  LDD1607  [2]
 LDCM0404  CL17 HEK-293T C56(1.01)  LDD1608  [2]
 LDCM0405  CL18 HEK-293T C325(0.96)  LDD1609  [2]
 LDCM0407  CL2 HEK-293T C325(1.04); C56(0.97)  LDD1611  [2]
 LDCM0408  CL20 HEK-293T C325(0.96)  LDD1612  [2]
 LDCM0409  CL21 HEK-293T C325(0.90); C56(1.23)  LDD1613  [2]
 LDCM0412  CL24 HEK-293T C325(1.01)  LDD1616  [2]
 LDCM0413  CL25 HEK-293T C325(0.89)  LDD1617  [2]
 LDCM0414  CL26 HEK-293T C325(0.93); C56(0.95)  LDD1618  [2]
 LDCM0415  CL27 HEK-293T C325(0.89); C56(0.98)  LDD1619  [2]
 LDCM0416  CL28 HEK-293T C325(0.67)  LDD1620  [2]
 LDCM0417  CL29 HEK-293T C56(0.96)  LDD1621  [2]
 LDCM0418  CL3 HEK-293T C325(1.09); C56(1.06)  LDD1622  [2]
 LDCM0419  CL30 HEK-293T C325(0.97)  LDD1623  [2]
 LDCM0421  CL32 HEK-293T C325(1.06)  LDD1625  [2]
 LDCM0422  CL33 HEK-293T C325(1.00); C56(1.33)  LDD1626  [2]
 LDCM0425  CL36 HEK-293T C325(1.14)  LDD1629  [2]
 LDCM0426  CL37 HEK-293T C325(1.05)  LDD1630  [2]
 LDCM0428  CL39 HEK-293T C325(0.95); C56(1.08)  LDD1632  [2]
 LDCM0429  CL4 HEK-293T C325(0.82)  LDD1633  [2]
 LDCM0430  CL40 HEK-293T C325(1.02)  LDD1634  [2]
 LDCM0431  CL41 HEK-293T C56(1.00)  LDD1635  [2]
 LDCM0432  CL42 HEK-293T C325(1.09)  LDD1636  [2]
 LDCM0434  CL44 HEK-293T C325(1.02)  LDD1638  [2]
 LDCM0435  CL45 HEK-293T C325(1.05); C56(1.09)  LDD1639  [2]
 LDCM0438  CL48 HEK-293T C325(1.21)  LDD1642  [2]
 LDCM0439  CL49 HEK-293T C325(0.87)  LDD1643  [2]
 LDCM0440  CL5 HEK-293T C56(1.03)  LDD1644  [2]
 LDCM0441  CL50 HEK-293T C325(0.93); C56(1.10)  LDD1645  [2]
 LDCM0443  CL52 HEK-293T C325(1.08)  LDD1646  [2]
 LDCM0444  CL53 HEK-293T C56(0.97)  LDD1647  [2]
 LDCM0445  CL54 HEK-293T C325(1.00)  LDD1648  [2]
 LDCM0447  CL56 HEK-293T C325(1.03)  LDD1650  [2]
 LDCM0448  CL57 HEK-293T C325(0.89); C56(1.10)  LDD1651  [2]
 LDCM0451  CL6 HEK-293T C325(1.04)  LDD1654  [2]
 LDCM0452  CL60 HEK-293T C325(0.96)  LDD1655  [2]
 LDCM0453  CL61 HEK-293T C325(0.89)  LDD1656  [2]
 LDCM0454  CL62 HEK-293T C325(1.09); C56(0.89)  LDD1657  [2]
 LDCM0455  CL63 HEK-293T C325(1.03); C56(0.96)  LDD1658  [2]
 LDCM0456  CL64 HEK-293T C325(1.09)  LDD1659  [2]
 LDCM0457  CL65 HEK-293T C56(0.96)  LDD1660  [2]
 LDCM0458  CL66 HEK-293T C325(1.01)  LDD1661  [2]
 LDCM0460  CL68 HEK-293T C325(1.04)  LDD1663  [2]
 LDCM0461  CL69 HEK-293T C325(1.17); C56(1.12)  LDD1664  [2]
 LDCM0465  CL72 HEK-293T C325(1.07)  LDD1668  [2]
 LDCM0466  CL73 HEK-293T C325(0.68)  LDD1669  [2]
 LDCM0467  CL74 HEK-293T C325(0.97); C56(0.99)  LDD1670  [2]
 LDCM0469  CL76 HEK-293T C325(0.98)  LDD1672  [2]
 LDCM0470  CL77 HEK-293T C56(0.93)  LDD1673  [2]
 LDCM0471  CL78 HEK-293T C325(1.04)  LDD1674  [2]
 LDCM0473  CL8 HEK-293T C325(1.15)  LDD1676  [2]
 LDCM0474  CL80 HEK-293T C325(1.03)  LDD1677  [2]
 LDCM0475  CL81 HEK-293T C325(1.02); C56(1.06)  LDD1678  [2]
 LDCM0478  CL84 HEK-293T C325(1.03)  LDD1681  [2]
 LDCM0479  CL85 HEK-293T C325(0.94)  LDD1682  [2]
 LDCM0480  CL86 HEK-293T C325(0.78); C56(0.96)  LDD1683  [2]
 LDCM0481  CL87 HEK-293T C325(1.01); C56(0.94)  LDD1684  [2]
 LDCM0482  CL88 HEK-293T C325(0.94)  LDD1685  [2]
 LDCM0483  CL89 HEK-293T C56(0.97)  LDD1686  [2]
 LDCM0484  CL9 HEK-293T C325(1.04); C56(1.13)  LDD1687  [2]
 LDCM0485  CL90 HEK-293T C325(1.05)  LDD1688  [2]
 LDCM0487  CL92 HEK-293T C325(1.06)  LDD1690  [2]
 LDCM0488  CL93 HEK-293T C325(0.97); C56(1.07)  LDD1691  [2]
 LDCM0491  CL96 HEK-293T C325(1.06)  LDD1694  [2]
 LDCM0492  CL97 HEK-293T C325(0.90)  LDD1695  [2]
 LDCM0493  CL98 HEK-293T C325(0.90); C56(1.04)  LDD1696  [2]
 LDCM0494  CL99 HEK-293T C325(1.13); C56(1.01)  LDD1697  [2]
 LDCM0495  E2913 HEK-293T C325(0.96); C56(1.05)  LDD1698  [2]
 LDCM0468  Fragment33 HEK-293T C325(0.90); C56(0.99)  LDD1671  [2]
 LDCM0427  Fragment51 HEK-293T C325(0.91); C56(0.97)  LDD1631  [2]
 LDCM0022  KB02 HEK-293T C325(0.98)  LDD1492  [2]
 LDCM0023  KB03 HEK-293T C325(0.90)  LDD1497  [2]
 LDCM0024  KB05 HEK-293T C325(0.96)  LDD1502  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrial (ALKBH7) AlkB family Q9BT30
Testis-specific serine/threonine-protein kinase 3 (TSSK3) CAMK Ser/Thr protein kinase family Q96PN8
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Coiled-coil domain-containing protein 103 (CCDC103) CCDC103/PR46b family Q8IW40
Oxidative stress-induced growth inhibitor 1 (OSGIN1) OKL38 family Q9UJX0

References

1 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060