Details of the Target

General Information of Target

Target ID LDTP10153
Target Name Mitochondrial dynamics protein MID49 (MIEF2)
Gene Name MIEF2
Gene ID 125170
Synonyms
MID49; SMCR7; Mitochondrial dynamics protein MID49; Mitochondrial dynamics protein of 49 kDa; Mitochondrial elongation factor 2; Smith-Magenis syndrome chromosomal region candidate gene 7 protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
METPTPLPPVPASPTCNPAPRTIQIEFPQHSSSLLESLNRHRLEGKFCDVSLLVQGRELR
AHKAVLAAASPYFHDKLLLGDAPRLTLPSVIEADAFEGLLQLIYSGRLRLPLDALPAHLL
VASGLQMWQVVDQCSEILRELETSGGGISARGGNSYHALLSTTSSTGGWCIRSSPFQTPV
QSSASTESPASTESPVGGEGSELGEVLQIQVEEEEEEEEDDDDEDQGSATLSQTPQPQRV
SGVFPRPHGPHPLPMTATPRKLPEGESAPLELPAPPALPPKIFYIKQEPFEPKEEISGSG
TQPGGAKEETKVFSGGDTEGNGELGFLLPSGPGPTSGGGGPSWKPVDLHGNEILSGGGGP
GGAGQAVHGPVKLGGTPPADGKRFGCLCGKRFAVKPKRDRHIMLTFSLRPFGCGICNKRF
KLKHHLTEHMKTHAGALHACPHCGRRFRVHACFLRHRDLCKGQGWATAHWTYK
Target Bioclass
Other
Family
MID49/MID51 family
Subcellular location
Mitochondrion outer membrane
Function
Mitochondrial outer membrane protein involved in the regulation of mitochondrial organization. It is required for mitochondrial fission and promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity.
Uniprot ID
Q96C03
Ensemble ID
ENST00000323019.9
HGNC ID
HGNC:17920

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
FADU SNV: p.R365S .
JURKAT SNV: p.E231K .
MELHO SNV: p.S273R .
SHP77 SNV: p.R353C .
SKMEL3 SNV: p.S273R .
WM793 SNV: p.K260M .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C378(3.34)  LDD3325  [1]
IA-alkyne
 Probe Info  		C233(0.96)  LDD2207  [2]
IPM
 Probe Info  		C233(0.00); C367(0.00)  LDD2156  [3]
TFBX
 Probe Info  		N.A.  LDD0148  [4]
AOyne
 Probe Info  		15.00  LDD0443  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C367(1.00)  LDD1507  [6]
 LDCM0215  AC10 HEK-293T C367(1.01); C364(1.07)  LDD1508  [6]
 LDCM0226  AC11 HEK-293T C367(0.99)  LDD1509  [6]
 LDCM0237  AC12 HEK-293T C367(0.94)  LDD1510  [6]
 LDCM0259  AC14 HEK-293T C367(1.12); C364(1.16)  LDD1512  [6]
 LDCM0270  AC15 HEK-293T C364(0.85)  LDD1513  [6]
 LDCM0276  AC17 HEK-293T C367(0.96)  LDD1515  [6]
 LDCM0277  AC18 HEK-293T C367(0.89); C364(1.06)  LDD1516  [6]
 LDCM0278  AC19 HEK-293T C367(1.13)  LDD1517  [6]
 LDCM0279  AC2 HEK-293T C367(0.91); C364(1.42)  LDD1518  [6]
 LDCM0280  AC20 HEK-293T C367(1.10)  LDD1519  [6]
 LDCM0282  AC22 HEK-293T C367(0.99); C364(0.92)  LDD1521  [6]
 LDCM0283  AC23 HEK-293T C364(0.92)  LDD1522  [6]
 LDCM0285  AC25 HEK-293T C367(0.94)  LDD1524  [6]
 LDCM0286  AC26 HEK-293T C367(0.96); C364(1.06)  LDD1525  [6]
 LDCM0287  AC27 HEK-293T C367(1.00)  LDD1526  [6]
 LDCM0288  AC28 HEK-293T C367(0.98)  LDD1527  [6]
 LDCM0290  AC3 HEK-293T C367(0.92)  LDD1529  [6]
 LDCM0291  AC30 HEK-293T C367(1.08); C364(1.21)  LDD1530  [6]
 LDCM0292  AC31 HEK-293T C364(0.93)  LDD1531  [6]
 LDCM0294  AC33 HEK-293T C367(1.08)  LDD1533  [6]
 LDCM0295  AC34 HEK-293T C367(0.91); C364(1.07)  LDD1534  [6]
 LDCM0296  AC35 HEK-293T C367(0.97)  LDD1535  [6]
 LDCM0297  AC36 HEK-293T C367(1.05)  LDD1536  [6]
 LDCM0299  AC38 HEK-293T C367(1.14); C364(0.88)  LDD1538  [6]
 LDCM0300  AC39 HEK-293T C364(1.08)  LDD1539  [6]
 LDCM0301  AC4 HEK-293T C367(0.99)  LDD1540  [6]
 LDCM0303  AC41 HEK-293T C367(0.93)  LDD1542  [6]
 LDCM0304  AC42 HEK-293T C367(0.94); C364(0.93)  LDD1543  [6]
 LDCM0305  AC43 HEK-293T C367(1.01)  LDD1544  [6]
 LDCM0306  AC44 HEK-293T C367(1.05)  LDD1545  [6]
 LDCM0308  AC46 HEK-293T C367(1.14); C364(1.10)  LDD1547  [6]
 LDCM0309  AC47 HEK-293T C364(0.92)  LDD1548  [6]
 LDCM0311  AC49 HEK-293T C367(0.89)  LDD1550  [6]
 LDCM0313  AC50 HEK-293T C367(0.94); C364(1.22)  LDD1552  [6]
 LDCM0314  AC51 HEK-293T C367(1.08)  LDD1553  [6]
 LDCM0315  AC52 HEK-293T C367(1.06)  LDD1554  [6]
 LDCM0317  AC54 HEK-293T C367(1.13); C364(1.02)  LDD1556  [6]
 LDCM0318  AC55 HEK-293T C364(0.97)  LDD1557  [6]
 LDCM0320  AC57 HEK-293T C367(1.04)  LDD1559  [6]
 LDCM0321  AC58 HEK-293T C367(0.95); C364(1.11)  LDD1560  [6]
 LDCM0322  AC59 HEK-293T C367(0.94)  LDD1561  [6]
 LDCM0323  AC6 HEK-293T C367(1.04); C364(1.21)  LDD1562  [6]
 LDCM0324  AC60 HEK-293T C367(0.99)  LDD1563  [6]
 LDCM0326  AC62 HEK-293T C367(1.07); C364(1.08)  LDD1565  [6]
 LDCM0327  AC63 HEK-293T C364(1.01)  LDD1566  [6]
 LDCM0334  AC7 HEK-293T C364(0.90)  LDD1568  [6]
 LDCM0356  AKOS034007680 HEK-293T C367(0.99)  LDD1570  [6]
 LDCM0367  CL1 HEK-293T C367(1.01); C364(0.85)  LDD1571  [6]
 LDCM0368  CL10 HEK-293T C367(1.45); C364(1.03)  LDD1572  [6]
 LDCM0369  CL100 HEK-293T C367(0.99)  LDD1573  [6]
 LDCM0370  CL101 HEK-293T C367(1.12); C364(1.01)  LDD1574  [6]
 LDCM0371  CL102 HEK-293T C367(1.16)  LDD1575  [6]
 LDCM0372  CL103 HEK-293T C364(0.82)  LDD1576  [6]
 LDCM0373  CL104 HEK-293T C367(0.85)  LDD1577  [6]
 LDCM0374  CL105 HEK-293T C367(1.07); C364(1.03)  LDD1578  [6]
 LDCM0375  CL106 HEK-293T C367(1.02)  LDD1579  [6]
 LDCM0376  CL107 HEK-293T C364(0.99)  LDD1580  [6]
 LDCM0377  CL108 HEK-293T C367(0.82)  LDD1581  [6]
 LDCM0378  CL109 HEK-293T C367(1.00); C364(0.74)  LDD1582  [6]
 LDCM0379  CL11 HEK-293T C364(0.94)  LDD1583  [6]
 LDCM0380  CL110 HEK-293T C367(1.17)  LDD1584  [6]
 LDCM0381  CL111 HEK-293T C364(1.09)  LDD1585  [6]
 LDCM0382  CL112 HEK-293T C367(0.81)  LDD1586  [6]
 LDCM0383  CL113 HEK-293T C367(0.91); C364(0.91)  LDD1587  [6]
 LDCM0384  CL114 HEK-293T C367(1.15)  LDD1588  [6]
 LDCM0385  CL115 HEK-293T C364(0.93)  LDD1589  [6]
 LDCM0386  CL116 HEK-293T C367(0.95)  LDD1590  [6]
 LDCM0387  CL117 HEK-293T C367(1.23); C364(0.92)  LDD1591  [6]
 LDCM0388  CL118 HEK-293T C367(0.94)  LDD1592  [6]
 LDCM0389  CL119 HEK-293T C364(0.86)  LDD1593  [6]
 LDCM0391  CL120 HEK-293T C367(0.92)  LDD1595  [6]
 LDCM0392  CL121 HEK-293T C367(1.00); C364(0.90)  LDD1596  [6]
 LDCM0393  CL122 HEK-293T C367(1.02)  LDD1597  [6]
 LDCM0394  CL123 HEK-293T C364(1.00)  LDD1598  [6]
 LDCM0395  CL124 HEK-293T C367(0.94)  LDD1599  [6]
 LDCM0396  CL125 HEK-293T C367(1.16); C364(1.23)  LDD1600  [6]
 LDCM0397  CL126 HEK-293T C367(1.19)  LDD1601  [6]
 LDCM0398  CL127 HEK-293T C364(1.19)  LDD1602  [6]
 LDCM0399  CL128 HEK-293T C367(0.89)  LDD1603  [6]
 LDCM0400  CL13 HEK-293T C367(0.95); C364(0.96)  LDD1604  [6]
 LDCM0401  CL14 HEK-293T C367(0.85)  LDD1605  [6]
 LDCM0402  CL15 HEK-293T C364(1.01)  LDD1606  [6]
 LDCM0403  CL16 HEK-293T C367(0.88)  LDD1607  [6]
 LDCM0404  CL17 HEK-293T C367(0.92)  LDD1608  [6]
 LDCM0405  CL18 HEK-293T C367(1.03); C364(0.90)  LDD1609  [6]
 LDCM0406  CL19 HEK-293T C367(1.01)  LDD1610  [6]
 LDCM0407  CL2 HEK-293T C367(1.01)  LDD1611  [6]
 LDCM0408  CL20 HEK-293T C367(1.04)  LDD1612  [6]
 LDCM0410  CL22 HEK-293T C367(1.28); C364(1.05)  LDD1614  [6]
 LDCM0411  CL23 HEK-293T C364(1.04)  LDD1615  [6]
 LDCM0413  CL25 HEK-293T C367(1.14); C364(1.02)  LDD1617  [6]
 LDCM0414  CL26 HEK-293T C367(1.02)  LDD1618  [6]
 LDCM0415  CL27 HEK-293T C364(1.06)  LDD1619  [6]
 LDCM0416  CL28 HEK-293T C367(0.96)  LDD1620  [6]
 LDCM0417  CL29 HEK-293T C367(1.02)  LDD1621  [6]
 LDCM0418  CL3 HEK-293T C364(1.10)  LDD1622  [6]
 LDCM0419  CL30 HEK-293T C367(0.90); C364(1.21)  LDD1623  [6]
 LDCM0420  CL31 HEK-293T C367(1.04)  LDD1624  [6]
 LDCM0421  CL32 HEK-293T C367(0.93)  LDD1625  [6]
 LDCM0423  CL34 HEK-293T C367(1.25); C364(1.12)  LDD1627  [6]
 LDCM0424  CL35 HEK-293T C364(0.96)  LDD1628  [6]
 LDCM0426  CL37 HEK-293T C367(1.11); C364(0.76)  LDD1630  [6]
 LDCM0428  CL39 HEK-293T C364(1.21)  LDD1632  [6]
 LDCM0429  CL4 HEK-293T C367(1.17)  LDD1633  [6]
 LDCM0430  CL40 HEK-293T C367(0.96)  LDD1634  [6]
 LDCM0431  CL41 HEK-293T C367(0.93)  LDD1635  [6]
 LDCM0432  CL42 HEK-293T C367(1.00); C364(1.25)  LDD1636  [6]
 LDCM0433  CL43 HEK-293T C367(0.97)  LDD1637  [6]
 LDCM0434  CL44 HEK-293T C367(1.25)  LDD1638  [6]
 LDCM0436  CL46 HEK-293T C367(1.13); C364(1.27)  LDD1640  [6]
 LDCM0437  CL47 HEK-293T C364(1.21)  LDD1641  [6]
 LDCM0439  CL49 HEK-293T C367(1.08); C364(1.29)  LDD1643  [6]
 LDCM0440  CL5 HEK-293T C367(1.01)  LDD1644  [6]
 LDCM0441  CL50 HEK-293T C367(0.93)  LDD1645  [6]
 LDCM0443  CL52 HEK-293T C367(1.01)  LDD1646  [6]
 LDCM0444  CL53 HEK-293T C367(1.16)  LDD1647  [6]
 LDCM0445  CL54 HEK-293T C367(1.23); C364(0.89)  LDD1648  [6]
 LDCM0446  CL55 HEK-293T C367(0.97)  LDD1649  [6]
 LDCM0447  CL56 HEK-293T C367(1.11)  LDD1650  [6]
 LDCM0449  CL58 HEK-293T C367(1.24); C364(0.99)  LDD1652  [6]
 LDCM0450  CL59 HEK-293T C364(1.07)  LDD1653  [6]
 LDCM0451  CL6 HEK-293T C367(1.21); C364(0.76)  LDD1654  [6]
 LDCM0453  CL61 HEK-293T C367(1.05); C364(1.29)  LDD1656  [6]
 LDCM0454  CL62 HEK-293T C367(1.12)  LDD1657  [6]
 LDCM0455  CL63 HEK-293T C364(0.97)  LDD1658  [6]
 LDCM0456  CL64 HEK-293T C367(1.00)  LDD1659  [6]
 LDCM0457  CL65 HEK-293T C367(0.98)  LDD1660  [6]
 LDCM0458  CL66 HEK-293T C367(0.97); C364(1.10)  LDD1661  [6]
 LDCM0459  CL67 HEK-293T C367(1.05)  LDD1662  [6]
 LDCM0460  CL68 HEK-293T C367(1.03)  LDD1663  [6]
 LDCM0462  CL7 HEK-293T C367(0.97)  LDD1665  [6]
 LDCM0463  CL70 HEK-293T C367(1.12); C364(1.14)  LDD1666  [6]
 LDCM0464  CL71 HEK-293T C364(0.99)  LDD1667  [6]
 LDCM0466  CL73 HEK-293T C367(1.00); C364(1.80)  LDD1669  [6]
 LDCM0467  CL74 HEK-293T C367(1.06)  LDD1670  [6]
 LDCM0469  CL76 HEK-293T C367(0.96)  LDD1672  [6]
 LDCM0470  CL77 HEK-293T C367(1.00)  LDD1673  [6]
 LDCM0471  CL78 HEK-293T C367(0.92); C364(0.93)  LDD1674  [6]
 LDCM0472  CL79 HEK-293T C367(0.89)  LDD1675  [6]
 LDCM0473  CL8 HEK-293T C367(1.93)  LDD1676  [6]
 LDCM0474  CL80 HEK-293T C367(1.02)  LDD1677  [6]
 LDCM0476  CL82 HEK-293T C367(1.11); C364(1.32)  LDD1679  [6]
 LDCM0477  CL83 HEK-293T C364(0.71)  LDD1680  [6]
 LDCM0479  CL85 HEK-293T C367(1.21); C364(0.89)  LDD1682  [6]
 LDCM0480  CL86 HEK-293T C367(0.98)  LDD1683  [6]
 LDCM0481  CL87 HEK-293T C364(0.97)  LDD1684  [6]
 LDCM0482  CL88 HEK-293T C367(1.11)  LDD1685  [6]
 LDCM0483  CL89 HEK-293T C367(1.04)  LDD1686  [6]
 LDCM0485  CL90 HEK-293T C367(1.49); C364(1.09)  LDD1688  [6]
 LDCM0486  CL91 HEK-293T C367(0.94)  LDD1689  [6]
 LDCM0487  CL92 HEK-293T C367(1.33)  LDD1690  [6]
 LDCM0489  CL94 HEK-293T C367(1.03); C364(1.23)  LDD1692  [6]
 LDCM0490  CL95 HEK-293T C364(0.93)  LDD1693  [6]
 LDCM0492  CL97 HEK-293T C367(1.14); C364(0.92)  LDD1695  [6]
 LDCM0493  CL98 HEK-293T C367(0.93)  LDD1696  [6]
 LDCM0494  CL99 HEK-293T C364(0.92)  LDD1697  [6]
 LDCM0495  E2913 HEK-293T C364(0.66)  LDD1698  [6]
 LDCM0468  Fragment33 HEK-293T C364(0.95)  LDD1671  [6]
 LDCM0427  Fragment51 HEK-293T C367(1.05)  LDD1631  [6]
 LDCM0022  KB02 A101D C378(3.04)  LDD2250  [1]
 LDCM0023  KB03 A2058 C378(3.07)  LDD2670  [1]
 LDCM0024  KB05 UACC257 C378(3.34)  LDD3325  [1]
 LDCM0628  OTUB2-COV-1 HEK-293T C233(0.96)  LDD2207  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Caspase-6 (CASP6) Peptidase C14A family P55212
17-beta-hydroxysteroid dehydrogenase 13 (HSD17B13) Short-chain dehydrogenases/reductases (SDR) family Q7Z5P4
Dynamin-1-like protein (DNM1L) Dynamin/Fzo/YdjA family O00429
Peptidyl-prolyl cis-trans isomerase FKBP7 (FKBP7) . Q9Y680
Transporter and channel
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Endophilin-B1 (SH3GLB1) Endophilin family Q9Y371
ATP synthase-coupling factor 6, mitochondrial (ATP5PF) Eukaryotic ATPase subunit F6 family P18859
Prenylated Rab acceptor protein 1 (RABAC1) PRA1 family Q9UI14
Synaptophysin (SYP) Synaptophysin/synaptobrevin family P08247
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger protein 628 (ZNF628) . Q5EBL2
Other
Click To Hide/Show 14 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
ADP-ribosylation factor-like protein 6-interacting protein 1 (ARL6IP1) ARL6ip family Q15041
Protein FAM114A2 (FAM114A2) FAM114 family Q9NRY5
Protein FAM25C (FAM25C) FAM25 family B3EWG5
Midkine (MDK) Pleiotrophin family P21741
Neurocalcin-delta (NCALD) Recoverin family P61601
Neuronal calcium sensor 1 (NCS1) Recoverin family P62166
Vesicle transport protein SFT2A (SFT2D1) SFT2 family Q8WV19
Protein YIF1A (YIF1A) YIF1 family O95070
Arfaptin-2 (ARFIP2) . P53365
Basic salivary proline-rich protein 2 (PRB2) . P02812
Lck-interacting transmembrane adapter 1 (LIME1) . Q9H400
Type-1 angiotensin II receptor-associated protein (AGTRAP) . Q6RW13
Ubiquilin-1 (UBQLN1) . Q9UMX0
Ubiquilin-2 (UBQLN2) . Q9UHD9

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.
3 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
4 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
5 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402