Details of the Target

General Information of Target

Target ID LDTP09815
Target Name Dedicator of cytokinesis protein 2 (DOCK2)
Gene Name DOCK2
Gene ID 1794
Synonyms
KIAA0209; Dedicator of cytokinesis protein 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAITLEEAPWLGWLLVKALMRFAFMVVNNLVAIPSYICYVIILQPLRVLDSKRFWYIEGI
MYKWLLGMVASWGWYAGYTVMEWGEDIKAVSKDEAVMLVNHQATGDVCTLMMCLQDKGLV
VAQMMWLMDHIFKYTNFGIVSLVHGDFFIRQGRSYRDQQLLLLKKHLENNYRSRDRKWIV
LFPEGGFLRKRRETSQAFAKKNNLPFLTNVTLPRSGATKIILNALVAQQKNGSPAGGDAK
ELDSKSKGLQWIIDTTIAYPKAEPIDIQTWILGYRKPTVTHVHYRIFPIKDVPLETDDLT
TWLYQRFVEKEDLLSHFYETGAFPPSKGHKEAVSREMTLSNLWIFLIQSFAFLSGYMWYN
IIQYFYHCLF
Target Bioclass
Other
Family
DOCK family
Subcellular location
Endomembrane system
Function
Involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. Activates RAC1 and RAC2, but not CDC42, by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. May also participate in IL2 transcriptional activation via the activation of RAC2.
Uniprot ID
Q92608
Ensemble ID
ENST00000520908.7
HGNC ID
HGNC:2988
ChEMBL ID
CHEMBL4105810

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.R301W .
8305C SNV: p.Y1281C .
A375 SNV: p.D33H DBIA    Probe Info 
A3KAW Insertion: .
SNV: .		 DBIA    Probe Info 
BICR22 SNV: p.S1392F .
CCK81 SNV: p.I1004V .
CCLP1 SNV: p.S1665T .
CORL88 SNV: p.W98Ter .
DU145 SNV: p.R854P; p.Y1188Ter .
HCT116 SNV: p.N1020I; p.D1536N .
HCT15 SNV: p.F822L; p.S1254L; p.T1390A .
HT115 SNV: p.T40P; p.L1185M .
LN18 SNV: p.L55I .
MCC26 SNV: p.D555N .
MEWO SNV: p.D309N; p.G554E; p.P1394L .
MFE319 SNV: p.Q443Ter .
MOLT4 SNV: p.E39D IA-alkyne    Probe Info 
NCIH1993 SNV: p.E68Ter .
NCIH358 SNV: p.V117E; p.A186S; p.V452L; p.S1445Y; p.Q1515H .
OV90 SNV: p.Q443K .
PEO1 SNV: p.L1510M .
RL SNV: p.L683P DBIA    Probe Info 
SG231 SNV: p.A496S .
SKMEL2 SNV: p.F1015L; p.A1259E .
SKN SNV: p.N171S .
SNGM SNV: p.M1192V .
SNU1 Deletion: p.Q26SfsTer25 .
SUDHL4 SNV: p.I684N DBIA    Probe Info 
SUDHL6 SNV: p.K1744M DBIA    Probe Info 
SW1573 SNV: p.F1551V .
SW480 SNV: p.S596P .
SW620 SNV: p.S596P .
TE1 SNV: p.L1572R .
WM115 Deletion: p.K1422SfsTer8 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
P8
 Probe Info  		10.00  LDD0455  [1]
TH211
 Probe Info  		Y438(20.00)  LDD0260  [2]
STPyne
 Probe Info  		K578(0.07)  LDD0277  [3]
DBIA
 Probe Info  		C730(17.22)  LDD0209  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		C1117(0.00); C1234(0.00); C607(0.00); C730(0.00)  LDD0038  [5]
IA-alkyne
 Probe Info  		C1117(0.00); C297(0.00); C1234(0.00); C607(0.00)  LDD0036  [5]
IPIAA_H
 Probe Info  		N.A.  LDD0030  [6]
IPIAA_L
 Probe Info  		C41(0.00); C1234(0.00); C1408(0.00)  LDD0031  [6]
Lodoacetamide azide
 Probe Info  		C1117(0.00); C1234(0.00); C607(0.00); C1160(0.00)  LDD0037  [5]
Compound 10
 Probe Info  		C1408(0.00); C730(0.00)  LDD2216  [7]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
VE-P
 Probe Info  		N.A.  LDD0396  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0198  Dimethyl Fumarate(DMF) T cell C1083(5.00)  LDD0513  [9]
 LDCM0625  F8 Ramos C41(1.39); C730(1.10); C1408(0.67); C1258(0.98)  LDD2187  [10]
 LDCM0572  Fragment10 Ramos C41(2.26); C1408(0.70)  LDD2189  [10]
 LDCM0573  Fragment11 Ramos C41(0.43); C1408(0.24); C1258(5.66)  LDD2190  [10]
 LDCM0574  Fragment12 Ramos C41(1.84); C1408(0.66)  LDD2191  [10]
 LDCM0575  Fragment13 Ramos C41(1.06); C730(0.59); C1408(0.75); C1258(0.83)  LDD2192  [10]
 LDCM0576  Fragment14 Ramos C41(1.75); C1408(1.40)  LDD2193  [10]
 LDCM0579  Fragment20 Ramos C41(2.82); C1408(0.61)  LDD2194  [10]
 LDCM0580  Fragment21 Ramos C41(1.10); C730(0.77); C1408(1.08); C1258(1.06)  LDD2195  [10]
 LDCM0582  Fragment23 Ramos C41(0.78); C1408(0.92); C1258(0.69)  LDD2196  [10]
 LDCM0578  Fragment27 Ramos C41(0.99); C730(0.68); C1408(1.01); C1258(1.33)  LDD2197  [10]
 LDCM0586  Fragment28 Ramos C41(0.87); C730(1.16); C1258(1.31)  LDD2198  [10]
 LDCM0588  Fragment30 Ramos C41(1.34); C730(1.67); C1408(0.71); C1258(0.45)  LDD2199  [10]
 LDCM0589  Fragment31 Ramos C41(0.97); C730(1.07); C1258(0.94)  LDD2200  [10]
 LDCM0590  Fragment32 Ramos C41(1.62); C1408(0.58); C1258(0.61)  LDD2201  [10]
 LDCM0468  Fragment33 Ramos C41(1.25); C730(0.94); C1408(0.77); C1258(1.37)  LDD2202  [10]
 LDCM0596  Fragment38 Ramos C41(1.23); C730(1.66); C1408(0.66); C1258(1.82)  LDD2203  [10]
 LDCM0566  Fragment4 Ramos C41(1.41); C1408(0.61)  LDD2184  [10]
 LDCM0610  Fragment52 Ramos C41(1.50); C730(1.24); C1258(0.89)  LDD2204  [10]
 LDCM0614  Fragment56 Ramos C41(1.09); C730(0.67); C1408(0.87); C1258(0.46)  LDD2205  [10]
 LDCM0569  Fragment7 Ramos C41(1.89); C1258(1.28)  LDD2186  [10]
 LDCM0571  Fragment9 Ramos C41(4.32); C1408(0.62); C1258(0.90)  LDD2188  [10]
 LDCM0022  KB02 Ramos C41(2.18); C1408(0.69)  LDD2182  [10]
 LDCM0023  KB03 Jurkat C730(17.22)  LDD0209  [4]
 LDCM0024  KB05 MOLM-13 C730(9.53); C41(2.47); C297(1.88); C1203(2.55)  LDD3333  [11]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Phospholipid scramblase 1 (PLSCR1) Phospholipid scramblase family O15162
E3 ubiquitin-protein ligase TRIM23 (TRIM23) Arf family P36406
Ras-related C3 botulinum toxin substrate 1 (RAC1) Rho family P63000
Protein-glutamine gamma-glutamyltransferase K (TGM1) Transglutaminase family P22735
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
MyoD family inhibitor (MDFI) MDFI family Q99750
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Homeobox protein Hox-A1 (HOXA1) Antp homeobox family P49639
Other
Click To Hide/Show 33 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Calcium-binding and coiled-coil domain-containing protein 2 (CALCOCO2) CALCOCO family Q13137
Cyclin-D1-binding protein 1 (CCNDBP1) CCNDBP1 family O95273
Lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) CDIP1/LITAF family Q99732
Cysteine-rich tail protein 1 (CYSRT1) CYSRT1 family A8MQ03
Golgin subfamily A member 6-like protein 9 (GOLGA6L9) GOLGA6 family A6NEM1
Keratin, type I cuticular Ha1 (KRT31) Intermediate filament family Q15323
Keratin, type I cuticular Ha4 (KRT34) Intermediate filament family O76011
Keratin, type I cytoskeletal 40 (KRT40) Intermediate filament family Q6A162
KH domain-containing, RNA-binding, signal transduction-associated protein 2 (KHDRBS2) KHDRBS family Q5VWX1
KH domain-containing, RNA-binding, signal transduction-associated protein 3 (KHDRBS3) KHDRBS family O75525
Keratin-associated protein 1-1 (KRTAP1-1) KRTAP type 1 family Q07627
Keratin-associated protein 1-3 (KRTAP1-3) KRTAP type 1 family Q8IUG1
Keratin-associated protein 10-7 (KRTAP10-7) KRTAP type 10 family P60409
Keratin-associated protein 10-8 (KRTAP10-8) KRTAP type 10 family P60410
Keratin-associated protein 10-9 (KRTAP10-9) KRTAP type 10 family P60411
Keratin-associated protein 12-3 (KRTAP12-3) KRTAP type 12 family P60328
Keratin-associated protein 3-1 (KRTAP3-1) KRTAP type 3 family Q9BYR8
Keratin-associated protein 3-3 (KRTAP3-3) KRTAP type 3 family Q9BYR6
Keratin-associated protein 4-2 (KRTAP4-2) KRTAP type 4 family Q9BYR5
Keratin-associated protein 5-7 (KRTAP5-7) KRTAP type 5 family Q6L8G8
Keratin-associated protein 5-9 (KRTAP5-9) KRTAP type 5 family P26371
Keratin-associated protein 9-2 (KRTAP9-2) KRTAP type 9 family Q9BYQ4
Keratin-associated protein 9-3 (KRTAP9-3) KRTAP type 9 family Q9BYQ3
Keratin-associated protein 9-4 (KRTAP9-4) KRTAP type 9 family Q9BYQ2
Microtubule-associated tumor suppressor candidate 2 (MTUS2) MTUS1 family Q5JR59
Notch homolog 2 N-terminal-like protein A (NOTCH2NLA) NOTCH family Q7Z3S9
Notch homolog 2 N-terminal-like protein C (NOTCH2NLC) NOTCH family P0DPK4
Teneurin-4 (TENM4) Tenascin family Q6N022
Caspase recruitment domain-containing protein 10 (CARD10) . Q9BWT7
Heterogeneous nuclear ribonucleoprotein K (HNRNPK) . P61978
Keratin-associated protein 17-1 (KRTAP17-1) . Q9BYP8
Microtubule-associated protein tau (MAPT) . P10636
Pleckstrin homology domain-containing family F member 2 (PLEKHF2) . Q9H8W4

References

1 Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors. Chembiochem. 2022 Dec 16;23(24):e202200389. doi: 10.1002/cbic.202200389. Epub 2022 Nov 22.
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
7 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
8 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
9 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
10 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
11 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840