Details of the Target

General Information of Target

Target ID LDTP09651
Target Name Choline transporter-like protein 1 (SLC44A1)
Gene Name SLC44A1
Gene ID 23446
Synonyms
CD92; CDW92; CTL1; Choline transporter-like protein 1; CDw92; Solute carrier family 44 member 1; CD antigen CD92
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGCCSSASSAAQSSKREWKPLEDRSCTDIPWLLLFILFCIGMGFICGFSIATGAAARLVS
GYDSYGNICGQKNTKLEAIPNSGMDHTQRKYVFFLDPCNLDLINRKIKSVALCVAACPRQ
ELKTLSDVQKFAEINGSALCSYNLKPSEYTTSPKSSVLCPKLPVPASAPIPFFHRCAPVN
ISCYAKFAEALITFVSDNSVLHRLISGVMTSKEIILGLCLLSLVLSMILMVIIRYISRVL
VWILTILVILGSLGGTGVLWWLYAKQRRSPKETVTPEQLQIAEDNLRALLIYAISATVFT
VILFLIMLVMRKRVALTIALFHVAGKVFIHLPLLVFQPFWTFFALVLFWVYWIMTLLFLG
TTGSPVQNEQGFVEFKISGPLQYMWWYHVVGLIWISEFILACQQMTVAGAVVTYYFTRDK
RNLPFTPILASVNRLIRYHLGTVAKGSFIITLVKIPRMILMYIHSQLKGKENACARCVLK
SCICCLWCLEKCLNYLNQNAYTATAINSTNFCTSAKDAFVILVENALRVATINTVGDFML
FLGKVLIVCSTGLAGIMLLNYQQDYTVWVLPLIIVCLFAFLVAHCFLSIYEMVVDVLFLC
FAIDTKYNDGSPGREFYMDKVLMEFVENSRKAMKEAGKGGVADSRELKPMASGASSA
Target Bioclass
Transporter and channel
Family
CTL (choline transporter-like) family
Subcellular location
Cell membrane
Function
Choline/H+ antiporter. Also acts as a high-affinity ethanolamine/H+ antiporter, regulating the supply of extracellular ethanolamine (Etn) for the CDP-Etn pathway, redistribute intracellular Etn and balance the CDP-Cho and CDP-Etn arms of the Kennedy pathway. Involved in membrane synthesis and myelin production.
Uniprot ID
Q8WWI5
Ensemble ID
ENST00000374720.8
HGNC ID
HGNC:18798

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CHL1 SNV: p.M461I .
HCC44 Substitution: p.S8T .
HEC1 SNV: p.S611N .
KMS12BM SNV: p.Q266K .
LNCaP clone FGC SNV: p.A111S .
LS123 SNV: p.I547T .
MEWO SNV: p.P456S .
NALM6 SNV: p.S137P .
OAW28 SNV: p.I306V .
OV56 Substitution: p.S8T .
RL SNV: p.L452F .
RL952 Deletion: p.L95WfsTer8 .
SKMEL5 Substitution: p.S8T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K90(3.72)  LDD0277  [1]
OPA-S-S-alkyne
 Probe Info  		K75(2.45)  LDD3494  [2]
DBIA
 Probe Info  		C159(2.94)  LDD3427  [3]
ART-yne
 Probe Info  		1.78  LDD0234  [4]
IA-alkyne
 Probe Info  		C98(0.00); C159(0.00); C69(0.00)  LDD0162  [5]
IPM
 Probe Info  		C4(0.00); C3(0.00)  LDD2156  [6]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C228
 Probe Info  		15.89  LDD1901  [7]
C376
 Probe Info  		6.73  LDD2036  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0259  AC14 HEK-293T C117(0.93)  LDD1512  [8]
 LDCM0282  AC22 HEK-293T C117(0.92)  LDD1521  [8]
 LDCM0291  AC30 HEK-293T C117(0.93)  LDD1530  [8]
 LDCM0299  AC38 HEK-293T C117(0.98)  LDD1538  [8]
 LDCM0308  AC46 HEK-293T C117(0.94)  LDD1547  [8]
 LDCM0317  AC54 HEK-293T C117(0.93)  LDD1556  [8]
 LDCM0323  AC6 HEK-293T C117(0.94)  LDD1562  [8]
 LDCM0326  AC62 HEK-293T C117(0.89)  LDD1565  [8]
 LDCM0091  Astemisinin MCF-7 1.78  LDD0234  [4]
 LDCM0368  CL10 HEK-293T C117(0.74)  LDD1572  [8]
 LDCM0410  CL22 HEK-293T C117(0.98)  LDD1614  [8]
 LDCM0423  CL34 HEK-293T C117(1.02)  LDD1627  [8]
 LDCM0436  CL46 HEK-293T C117(0.99)  LDD1640  [8]
 LDCM0449  CL58 HEK-293T C117(0.90)  LDD1652  [8]
 LDCM0463  CL70 HEK-293T C117(0.96)  LDD1666  [8]
 LDCM0476  CL82 HEK-293T C117(0.94)  LDD1679  [8]
 LDCM0489  CL94 HEK-293T C117(1.11)  LDD1692  [8]
 LDCM0572  Fragment10 Ramos 8.30  LDD2189  [9]
 LDCM0573  Fragment11 Ramos 6.44  LDD2190  [9]
 LDCM0574  Fragment12 Ramos 4.55  LDD2191  [9]
 LDCM0576  Fragment14 Ramos 0.75  LDD2193  [9]
 LDCM0580  Fragment21 Ramos 2.05  LDD2195  [9]
 LDCM0582  Fragment23 Ramos 10.01  LDD2196  [9]
 LDCM0578  Fragment27 Ramos 2.58  LDD2197  [9]
 LDCM0588  Fragment30 Ramos 2.84  LDD2199  [9]
 LDCM0468  Fragment33 Ramos 4.04  LDD2202  [9]
 LDCM0022  KB02 Ramos 0.94  LDD2182  [9]
 LDCM0023  KB03 Hs 936.T C159(4.67)  LDD2780  [3]
 LDCM0024  KB05 SK-MEL-28 C159(2.94)  LDD3427  [3]

The Interaction Atlas With This Target

The Drug(s) Related To This Target

Approved
Click To Hide/Show 2 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Choline Small molecular drug DB00122
Choline Salicylate Small molecular drug DB14006

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Artemisinin inhibits NRas palmitoylation by targeting the protein acyltransferase ZDHHC6. Cell Chem Biol. 2022 Mar 17;29(3):530-537.e7. doi: 10.1016/j.chembiol.2021.07.012. Epub 2021 Aug 5.
5 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
6 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
7 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
8 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
9 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578