Details of the Target

General Information of Target

Target ID LDTP09587
Target Name Protein BRICK1 (BRK1)
Gene Name BRK1
Gene ID 55845
Synonyms
C3orf10; Protein BRICK1; BRK1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAGQEDPVQREIHQDWANREYIEIITSSIKKIADFLNSFDMSCRSRLATLNEKLTALERR
IEYIEARVTKGETLT
Target Bioclass
Other
Family
BRK1 family
Subcellular location
Cytoplasm, cytoskeleton
Function
Involved in regulation of actin and microtubule organization. Part of a WAVE complex that activates the Arp2/3 complex. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes.
Uniprot ID
Q8WUW1
Ensemble ID
ENST00000530758.2
HGNC ID
HGNC:23057
ChEMBL ID
CHEMBL3758062

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 12 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C43(2.62)  LDD3333  [1]
Alkyne-RA190
 Probe Info  		3.57  LDD0299  [2]
Acrolein
 Probe Info  		N.A.  LDD0224  [3]
IA-alkyne
 Probe Info  		N.A.  LDD0175  [4]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [5]
NAIA_4
 Probe Info  		N.A.  LDD2226  [6]
TFBX
 Probe Info  		N.A.  LDD0027  [5]
WYneN
 Probe Info  		N.A.  LDD0021  [7]
IPM
 Probe Info  		N.A.  LDD2156  [8]
STPyne
 Probe Info  		N.A.  LDD0009  [7]
AOyne
 Probe Info  		14.80  LDD0443  [9]
NAIA_5
 Probe Info  		N.A.  LDD2223  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C43(0.86)  LDD1507  [10]
 LDCM0259  AC14 HEK-293T C43(0.97)  LDD1512  [10]
 LDCM0276  AC17 HEK-293T C43(1.00)  LDD1515  [10]
 LDCM0281  AC21 HEK-293T C43(0.90)  LDD1520  [10]
 LDCM0282  AC22 HEK-293T C43(0.98)  LDD1521  [10]
 LDCM0285  AC25 HEK-293T C43(0.91)  LDD1524  [10]
 LDCM0289  AC29 HEK-293T C43(0.97)  LDD1528  [10]
 LDCM0291  AC30 HEK-293T C43(0.97)  LDD1530  [10]
 LDCM0294  AC33 HEK-293T C43(1.03)  LDD1533  [10]
 LDCM0298  AC37 HEK-293T C43(1.01)  LDD1537  [10]
 LDCM0299  AC38 HEK-293T C43(1.03)  LDD1538  [10]
 LDCM0303  AC41 HEK-293T C43(1.12)  LDD1542  [10]
 LDCM0307  AC45 HEK-293T C43(1.15)  LDD1546  [10]
 LDCM0308  AC46 HEK-293T C43(1.03)  LDD1547  [10]
 LDCM0311  AC49 HEK-293T C43(1.11)  LDD1550  [10]
 LDCM0312  AC5 HEK-293T C43(1.05)  LDD1551  [10]
 LDCM0316  AC53 HEK-293T C43(1.00)  LDD1555  [10]
 LDCM0317  AC54 HEK-293T C43(1.06)  LDD1556  [10]
 LDCM0320  AC57 HEK-293T C43(1.05)  LDD1559  [10]
 LDCM0323  AC6 HEK-293T C43(1.00)  LDD1562  [10]
 LDCM0325  AC61 HEK-293T C43(0.99)  LDD1564  [10]
 LDCM0326  AC62 HEK-293T C43(1.06)  LDD1565  [10]
 LDCM0248  AKOS034007472 HEK-293T C43(0.97)  LDD1511  [10]
 LDCM0356  AKOS034007680 HEK-293T C43(1.10)  LDD1570  [10]
 LDCM0632  CL-Sc Hep-G2 C43(1.08)  LDD2227  [6]
 LDCM0368  CL10 HEK-293T C43(1.09)  LDD1572  [10]
 LDCM0369  CL100 HEK-293T C43(0.99)  LDD1573  [10]
 LDCM0372  CL103 HEK-293T C43(1.10)  LDD1576  [10]
 LDCM0373  CL104 HEK-293T C43(1.01)  LDD1577  [10]
 LDCM0376  CL107 HEK-293T C43(1.04)  LDD1580  [10]
 LDCM0377  CL108 HEK-293T C43(0.93)  LDD1581  [10]
 LDCM0381  CL111 HEK-293T C43(1.20)  LDD1585  [10]
 LDCM0382  CL112 HEK-293T C43(0.88)  LDD1586  [10]
 LDCM0385  CL115 HEK-293T C43(1.13)  LDD1589  [10]
 LDCM0386  CL116 HEK-293T C43(0.94)  LDD1590  [10]
 LDCM0389  CL119 HEK-293T C43(1.18)  LDD1593  [10]
 LDCM0391  CL120 HEK-293T C43(1.00)  LDD1595  [10]
 LDCM0394  CL123 HEK-293T C43(1.35)  LDD1598  [10]
 LDCM0395  CL124 HEK-293T C43(1.13)  LDD1599  [10]
 LDCM0398  CL127 HEK-293T C43(1.21)  LDD1602  [10]
 LDCM0399  CL128 HEK-293T C43(1.08)  LDD1603  [10]
 LDCM0402  CL15 HEK-293T C43(1.20)  LDD1606  [10]
 LDCM0403  CL16 HEK-293T C43(0.95)  LDD1607  [10]
 LDCM0404  CL17 HEK-293T C43(1.09)  LDD1608  [10]
 LDCM0409  CL21 HEK-293T C43(0.87)  LDD1613  [10]
 LDCM0410  CL22 HEK-293T C43(1.04)  LDD1614  [10]
 LDCM0415  CL27 HEK-293T C43(1.08)  LDD1619  [10]
 LDCM0416  CL28 HEK-293T C43(0.98)  LDD1620  [10]
 LDCM0417  CL29 HEK-293T C43(0.98)  LDD1621  [10]
 LDCM0418  CL3 HEK-293T C43(1.15)  LDD1622  [10]
 LDCM0422  CL33 HEK-293T C43(0.96)  LDD1626  [10]
 LDCM0423  CL34 HEK-293T C43(0.96)  LDD1627  [10]
 LDCM0428  CL39 HEK-293T C43(1.04)  LDD1632  [10]
 LDCM0429  CL4 HEK-293T C43(1.01)  LDD1633  [10]
 LDCM0430  CL40 HEK-293T C43(0.96)  LDD1634  [10]
 LDCM0431  CL41 HEK-293T C43(0.89)  LDD1635  [10]
 LDCM0435  CL45 HEK-293T C43(0.87)  LDD1639  [10]
 LDCM0436  CL46 HEK-293T C43(1.06)  LDD1640  [10]
 LDCM0440  CL5 HEK-293T C43(0.91)  LDD1644  [10]
 LDCM0443  CL52 HEK-293T C43(0.95)  LDD1646  [10]
 LDCM0444  CL53 HEK-293T C43(1.17)  LDD1647  [10]
 LDCM0448  CL57 HEK-293T C43(0.84)  LDD1651  [10]
 LDCM0449  CL58 HEK-293T C43(0.91)  LDD1652  [10]
 LDCM0455  CL63 HEK-293T C43(1.03)  LDD1658  [10]
 LDCM0456  CL64 HEK-293T C43(1.18)  LDD1659  [10]
 LDCM0457  CL65 HEK-293T C43(1.05)  LDD1660  [10]
 LDCM0461  CL69 HEK-293T C43(0.78)  LDD1664  [10]
 LDCM0463  CL70 HEK-293T C43(0.87)  LDD1666  [10]
 LDCM0469  CL76 HEK-293T C43(0.92)  LDD1672  [10]
 LDCM0470  CL77 HEK-293T C43(0.97)  LDD1673  [10]
 LDCM0475  CL81 HEK-293T C43(0.80)  LDD1678  [10]
 LDCM0476  CL82 HEK-293T C43(1.10)  LDD1679  [10]
 LDCM0481  CL87 HEK-293T C43(1.09)  LDD1684  [10]
 LDCM0482  CL88 HEK-293T C43(0.98)  LDD1685  [10]
 LDCM0483  CL89 HEK-293T C43(1.04)  LDD1686  [10]
 LDCM0484  CL9 HEK-293T C43(0.78)  LDD1687  [10]
 LDCM0488  CL93 HEK-293T C43(0.93)  LDD1691  [10]
 LDCM0489  CL94 HEK-293T C43(1.25)  LDD1692  [10]
 LDCM0494  CL99 HEK-293T C43(0.82)  LDD1697  [10]
 LDCM0495  E2913 HEK-293T C43(0.68)  LDD1698  [10]
 LDCM0625  F8 Ramos C43(0.47)  LDD2187  [11]
 LDCM0572  Fragment10 Ramos C43(1.05)  LDD2189  [11]
 LDCM0573  Fragment11 Ramos C43(0.17)  LDD2190  [11]
 LDCM0574  Fragment12 Ramos C43(1.07)  LDD2191  [11]
 LDCM0575  Fragment13 Ramos C43(0.78)  LDD2192  [11]
 LDCM0576  Fragment14 Ramos C43(1.75)  LDD2193  [11]
 LDCM0579  Fragment20 Ramos C43(0.82)  LDD2194  [11]
 LDCM0580  Fragment21 Ramos C43(0.77)  LDD2195  [11]
 LDCM0582  Fragment23 Ramos C43(0.87)  LDD2196  [11]
 LDCM0578  Fragment27 Ramos C43(0.70)  LDD2197  [11]
 LDCM0586  Fragment28 Ramos C43(0.56)  LDD2198  [11]
 LDCM0588  Fragment30 Ramos C43(1.45)  LDD2199  [11]
 LDCM0589  Fragment31 Ramos C43(0.86)  LDD2200  [11]
 LDCM0590  Fragment32 Ramos C43(0.79)  LDD2201  [11]
 LDCM0468  Fragment33 HEK-293T C43(1.05)  LDD1671  [10]
 LDCM0596  Fragment38 Ramos C43(1.02)  LDD2203  [11]
 LDCM0566  Fragment4 Ramos C43(1.53)  LDD2184  [11]
 LDCM0610  Fragment52 Ramos C43(0.95)  LDD2204  [11]
 LDCM0614  Fragment56 Ramos C43(0.85)  LDD2205  [11]
 LDCM0569  Fragment7 Ramos C43(0.73)  LDD2186  [11]
 LDCM0571  Fragment9 Ramos C43(0.72)  LDD2188  [11]
 LDCM0022  KB02 HEK-293T C43(0.97)  LDD1492  [10]
 LDCM0023  KB03 HEK-293T C43(1.13)  LDD1497  [10]
 LDCM0024  KB05 MOLM-13 C43(2.62)  LDD3333  [1]
 LDCM0109  NEM HeLa N.A.  LDD0224  [3]
 LDCM0131  RA190 MM1.R 3.57  LDD0299  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 10 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
E3 ubiquitin-protein ligase BRE1A (RNF20) BRE1 family Q5VTR2
Protein arginine N-methyltransferase 5 (PRMT5) Protein arginine N-methyltransferase family O14744
NADH dehydrogenase flavoprotein 2, mitochondrial (NDUFV2) Complex I 24 kDa subunit family P19404
Dopamine beta-hydroxylase (DBH) Copper type II ascorbate-dependent monooxygenase family P09172
Nucleoside diphosphate kinase, mitochondrial (NME4) NDK family O00746
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) Peptidase C12 family P09936
Ubiquitin thioesterase OTUB1 (OTUB1) Peptidase C65 family Q96FW1
Zinc-regulated GTPase metalloprotein activator 1A (ZNG1A) SIMIBI class G3E GTPase family Q9BRT8
Ubiquitin-conjugating enzyme E2 K (UBE2K) Ubiquitin-conjugating enzyme family P61086
Vitamin K epoxide reductase complex subunit 1-like protein 1 (VKORC1L1) VKOR family Q8N0U8
Transporter and channel
Click To Hide/Show 7 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Voltage-dependent anion-selective channel protein 2 (VDAC2) Eukaryotic mitochondrial porin family P45880
Huntingtin (HTT) Huntingtin family P42858
Junctophilin-3 (JPH3) Junctophilin family Q8WXH2
Lysosomal-associated transmembrane protein 4A (LAPTM4A) LAPTM4/LAPTM5 transporter family Q15012
Mitochondrial pyruvate carrier 2 (MPC2) Mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family O95563
Nucleoporin p58/p45 (NUP58) NUP58 family Q9BVL2
Alpha-synuclein (SNCA) Synuclein family P37840
Transcription factor
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
SAM pointed domain-containing Ets transcription factor (SPDEF) ETS family O95238
PHD finger protein 19 (PHF19) Polycomblike family Q5T6S3
Krueppel-like factor 11 (KLF11) Sp1 C2H2-type zinc-finger protein family O14901
GPCR
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
P2Y purinoceptor 2 (P2RY2) G-protein coupled receptor 1 family P41231
Other
Click To Hide/Show 23 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Hsp90 co-chaperone Cdc37-like 1 (CDC37L1) CDC37 family Q7L3B6
Cilia- and flagella-associated protein 68 (CFAP68) CFAP68 family Q9H5F2
Dysbindin (DTNBP1) Dysbindin family Q96EV8
Progranulin (GRN) Granulin family P28799
Heat shock factor-binding protein 1 (HSBP1) HSBP1 family O75506
Glial fibrillary acidic protein (GFAP) Intermediate filament family P14136
Peripherin (PRPH) Intermediate filament family P41219
MORF4 family-associated protein 1 (MRFAP1) MORF4 family-associated protein family Q9Y605
Nuclear distribution protein nudE-like 1 (NDEL1) NudE family Q9GZM8
Alpha-2-macroglobulin (A2M) Protease inhibitor I39 (alpha-2-macroglobulin) family P01023
Mitochondrial import inner membrane translocase subunit Tim8 A (TIMM8A) Small Tim family O60220
T-complex protein 1 subunit zeta (CCT6A) TCP-1 chaperonin family P40227
Sperm-egg fusion protein TMEM95 (TMEM95) TMEM95 family Q3KNT9
Zinc finger HIT domain-containing protein 1 (ZNHIT1) ZNHIT1 family O43257
Break repair meiotic recombinase recruitment factor 1 (BRME1) . Q0VDD7
Elongation factor 1-gamma (EEF1G) . P26641
Melanoma-associated antigen B6 (MAGEB6) . Q8N7X4
Methyl-CpG-binding protein 2 (MECP2) . P51608
Period circadian protein homolog 1 (PER1) . O15534
RING finger protein 11 (RNF11) . Q9Y3C5
RING1 and YY1-binding protein (RYBP) . Q8N488
Sprouty-related, EVH1 domain-containing protein 1 (SPRED1) . Q7Z699
WAP four-disulfide core domain protein 5 (WFDC5) . Q8TCV5

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.
3 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
6 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
7 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
8 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
9 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
10 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
11 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578