Details of the Target

General Information of Target

Target ID LDTP09533
Target Name Protein Shroom3 (SHROOM3)
Gene Name SHROOM3
Gene ID 57619
Synonyms
KIAA1481; SHRML; Protein Shroom3; Shroom-related protein; hShrmL
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MMRTTEDFHKPSATLNSNTATKGRYIYLEAFLEGGAPWGFTLKGGLEHGEPLIISKVEEG
GKADTLSSKLQAGDEVVHINEVTLSSSRKEAVSLVKGSYKTLRLVVRRDVCTDPGHADTG
ASNFVSPEHLTSGPQHRKAAWSGGVKLRLKHRRSEPAGRPHSWHTTKSGEKQPDASMMQI
SQGMIGPPWHQSYHSSSSTSDLSNYDHAYLRRSPDQCSSQGSMESLEPSGAYPPCHLSPA
KSTGSIDQLSHFHNKRDSAYSSFSTSSSILEYPHPGISGRERSGSMDNTSARGGLLEGMR
QADIRYVKTVYDTRRGVSAEYEVNSSALLLQGREARASANGQGYDKWSNIPRGKGVPPPS
WSQQCPSSLETATDNLPPKVGAPLPPARSDSYAAFRHRERPSSWSSLDQKRLCRPQANSL
GSLKSPFIEEQLHTVLEKSPENSPPVKPKHNYTQKAQPGQPLLPTSIYPVPSLEPHFAQV
PQPSVSSNGMLYPALAKESGYIAPQGACNKMATIDENGNQNGSGRPGFAFCQPLEHDLLS
PVEKKPEATAKYVPSKVHFCSVPENEEDASLKRHLTPPQGNSPHSNERKSTHSNKPSSHP
HSLKCPQAQAWQAGEDKRSSRLSEPWEGDFQEDHNANLWRRLEREGLGQSLSGNFGKTKS
AFSSLQNIPESLRRHSSLELGRGTQEGYPGGRPTCAVNTKAEDPGRKAAPDLGSHLDRQV
SYPRPEGRTGASASFNSTDPSPEEPPAPSHPHTSSLGRRGPGPGSASALQGFQYGKPHCS
VLEKVSKFEQREQGSQRPSVGGSGFGHNYRPHRTVSTSSTSGNDFEETKAHIRFSESAEP
LGNGEQHFKNGELKLEEASRQPCGQQLSGGASDSGRGPQRPDARLLRSQSTFQLSSEPER
EPEWRDRPGSPESPLLDAPFSRAYRNSIKDAQSRVLGATSFRRRDLELGAPVASRSWRPR
PSSAHVGLRSPEASASASPHTPRERHSVTPAEGDLARPVPPAARRGARRRLTPEQKKRSY
SEPEKMNEVGIVEEAEPAPLGPQRNGMRFPESSVADRRRLFERDGKACSTLSLSGPELKQ
FQQSALADYIQRKTGKRPTSAAGCSLQEPGPLRERAQSAYLQPGPAALEGSGLASASSLS
SLREPSLQPRREATLLPATVAETQQAPRDRSSSFAGGRRLGERRRGDLLSGANGGTRGTQ
RGDETPREPSSWGARAGKSMSAEDLLERSDVLAGPVHVRSRSSPATADKRQDVLLGQDSG
FGLVKDPCYLAGPGSRSLSCSERGQEEMLPLFHHLTPRWGGSGCKAIGDSSVPSECPGTL
DHQRQASRTPCPRPPLAGTQGLVTDTRAAPLTPIGTPLPSAIPSGYCSQDGQTGRQPLPP
YTPAMMHRSNGHTLTQPPGPRGCEGDGPEHGVEEGTRKRVSLPQWPPPSRAKWAHAARED
SLPEESSAPDFANLKHYQKQQSLPSLCSTSDPDTPLGAPSTPGRISLRISESVLRDSPPP
HEDYEDEVFVRDPHPKATSSPTFEPLPPPPPPPPSQETPVYSMDDFPPPPPHTVCEAQLD
SEDPEGPRPSFNKLSKVTIARERHMPGAAHVVGSQTLASRLQTSIKGSEAESTPPSFMSV
HAQLAGSLGGQPAPIQTQSLSHDPVSGTQGLEKKVSPDPQKSSEDIRTEALAKEIVHQDK
SLADILDPDSRLKTTMDLMEGLFPRDVNLLKENSVKRKAIQRTVSSSGCEGKRNEDKEAV
SMLVNCPAYYSVSAPKAELLNKIKEMPAEVNEEEEQADVNEKKAELIGSLTHKLETLQEA
KGSLLTDIKLNNALGEEVEALISELCKPNEFDKYRMFIGDLDKVVNLLLSLSGRLARVEN
VLSGLGEDASNEERSSLYEKRKILAGQHEDARELKENLDRRERVVLGILANYLSEEQLQD
YQHFVKMKSTLLIEQRKLDDKIKLGQEQVKCLLESLPSDFIPKAGALALPPNLTSEPIPA
GGCTFSGIFPTLTSPL
Target Bioclass
Other
Family
Shroom family
Subcellular location
Cell junction, adherens junction
Function
Controls cell shape changes in the neuroepithelium during neural tube closure. Induces apical constriction in epithelial cells by promoting the apical accumulation of F-actin and myosin II, and probably by bundling stress fibers. Induces apicobasal cell elongation by redistributing gamma-tubulin and directing the assembly of robust apicobasal microtubule arrays.
Uniprot ID
Q8TF72
Ensemble ID
ENST00000296043.7
HGNC ID
HGNC:30422

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA SNV: p.R1048H DBIA    Probe Info 
C4II SNV: p.S1368L DBIA    Probe Info 
CAL120 SNV: p.R1722S .
CAMA1 SNV: p.Q865Ter .
CCSW1 SNV: p.P1527T DBIA    Probe Info 
DU145 SNV: p.Q1917H .
DU4475 SNV: p.R1009W .
HEC1 SNV: p.R1903Ter DBIA    Probe Info 
KURAMOCHI SNV: p.R1179L DBIA    Probe Info 
LS180 SNV: p.Q932Ter DBIA    Probe Info 
MCC13 SNV: p.G23R .
MFE296 SNV: p.R1705Q .
MOLT4 SNV: p.R791Ter; p.P1704L .
NALM6 SNV: p.R1008H .
NCIH1792 SNV: p.R1168L .
OCUG1 SNV: p.H1237R DBIA    Probe Info 
PC14 SNV: p.S1599Y .
PC9 SNV: p.S1599Y .
PF382 SNV: p.G1371S .
RCC10RGB SNV: p.T1319P .
RL952 SNV: p.Q364Ter .
SKMEL30 SNV: p.S835F .
SNGM SNV: p.P704T .
SW620 SNV: p.W38Ter DBIA    Probe Info 
TE4 Substitution: p.G967L .
WM1552C SNV: p.R985Q .
WM2664 Insertion: p.P1529TfsTer14 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
YN-1
 Probe Info  		100.00  LDD0444  [1]
STPyne
 Probe Info  		K1880(2.00); K1943(6.48); K308(5.61); K657(10.00)  LDD0277  [2]
DBIA
 Probe Info  		C1403(2.14); C1104(2.27)  LDD3337  [3]
IA-alkyne
 Probe Info  		N.A.  LDD0163  [4]
NAIA_5
 Probe Info  		N.A.  LDD2224  [5]
IPM
 Probe Info  		C1331(0.00); C1104(0.00); C508(0.00); C695(0.00)  LDD0147  [6]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C508(1.12)  LDD1508  [8]
 LDCM0226  AC11 HEK-293T C508(0.98); C1104(1.05)  LDD1509  [8]
 LDCM0237  AC12 HEK-293T C1068(1.29)  LDD1510  [8]
 LDCM0259  AC14 HEK-293T C560(1.64)  LDD1512  [8]
 LDCM0270  AC15 HEK-293T C508(1.06)  LDD1513  [8]
 LDCM0277  AC18 HEK-293T C508(0.97)  LDD1516  [8]
 LDCM0278  AC19 HEK-293T C508(0.86); C1104(1.41)  LDD1517  [8]
 LDCM0279  AC2 HEK-293T C508(1.25)  LDD1518  [8]
 LDCM0280  AC20 HEK-293T C1068(1.18)  LDD1519  [8]
 LDCM0282  AC22 HEK-293T C560(1.13)  LDD1521  [8]
 LDCM0283  AC23 HEK-293T C508(0.92)  LDD1522  [8]
 LDCM0286  AC26 HEK-293T C508(1.22)  LDD1525  [8]
 LDCM0287  AC27 HEK-293T C508(1.04); C1104(1.10)  LDD1526  [8]
 LDCM0288  AC28 HEK-293T C1068(1.21)  LDD1527  [8]
 LDCM0290  AC3 HEK-293T C508(0.81); C1104(1.02)  LDD1529  [8]
 LDCM0291  AC30 HEK-293T C560(1.28)  LDD1530  [8]
 LDCM0292  AC31 HEK-293T C508(0.81)  LDD1531  [8]
 LDCM0295  AC34 HEK-293T C508(1.28)  LDD1534  [8]
 LDCM0296  AC35 HEK-293T C508(0.78); C1104(1.00)  LDD1535  [8]
 LDCM0297  AC36 HEK-293T C1068(1.14)  LDD1536  [8]
 LDCM0299  AC38 HEK-293T C560(1.33)  LDD1538  [8]
 LDCM0300  AC39 HEK-293T C508(1.08)  LDD1539  [8]
 LDCM0301  AC4 HEK-293T C1068(1.35)  LDD1540  [8]
 LDCM0304  AC42 HEK-293T C508(1.36)  LDD1543  [8]
 LDCM0305  AC43 HEK-293T C508(0.85); C1104(1.19)  LDD1544  [8]
 LDCM0306  AC44 HEK-293T C1068(1.20)  LDD1545  [8]
 LDCM0308  AC46 HEK-293T C560(1.22)  LDD1547  [8]
 LDCM0309  AC47 HEK-293T C508(1.14)  LDD1548  [8]
 LDCM0313  AC50 HEK-293T C508(1.12)  LDD1552  [8]
 LDCM0314  AC51 HEK-293T C508(0.75); C1104(1.00)  LDD1553  [8]
 LDCM0315  AC52 HEK-293T C1068(1.32)  LDD1554  [8]
 LDCM0317  AC54 HEK-293T C560(1.36)  LDD1556  [8]
 LDCM0318  AC55 HEK-293T C508(0.91)  LDD1557  [8]
 LDCM0321  AC58 HEK-293T C508(1.12)  LDD1560  [8]
 LDCM0322  AC59 HEK-293T C508(0.81); C1104(1.02)  LDD1561  [8]
 LDCM0323  AC6 HEK-293T C560(1.05)  LDD1562  [8]
 LDCM0324  AC60 HEK-293T C1068(1.00)  LDD1563  [8]
 LDCM0326  AC62 HEK-293T C560(0.99)  LDD1565  [8]
 LDCM0327  AC63 HEK-293T C508(0.97)  LDD1566  [8]
 LDCM0334  AC7 HEK-293T C508(1.08)  LDD1568  [8]
 LDCM0632  CL-Sc Hep-G2 C1403(0.75)  LDD2227  [5]
 LDCM0368  CL10 HEK-293T C560(1.11)  LDD1572  [8]
 LDCM0369  CL100 HEK-293T C1304(0.94); C508(1.11)  LDD1573  [8]
 LDCM0371  CL102 HEK-293T C1304(0.84)  LDD1575  [8]
 LDCM0373  CL104 HEK-293T C1304(0.94); C508(1.07)  LDD1577  [8]
 LDCM0375  CL106 HEK-293T C1304(0.89)  LDD1579  [8]
 LDCM0377  CL108 HEK-293T C1304(1.08); C508(1.05)  LDD1581  [8]
 LDCM0379  CL11 HEK-293T C508(1.03)  LDD1583  [8]
 LDCM0380  CL110 HEK-293T C1304(0.85)  LDD1584  [8]
 LDCM0382  CL112 HEK-293T C1304(0.89); C508(1.07)  LDD1586  [8]
 LDCM0384  CL114 HEK-293T C1304(0.69)  LDD1588  [8]
 LDCM0386  CL116 HEK-293T C1304(1.00); C508(1.26)  LDD1590  [8]
 LDCM0388  CL118 HEK-293T C1304(0.86)  LDD1592  [8]
 LDCM0391  CL120 HEK-293T C1304(1.15); C508(1.17)  LDD1595  [8]
 LDCM0393  CL122 HEK-293T C1304(0.90)  LDD1597  [8]
 LDCM0395  CL124 HEK-293T C1304(0.93); C508(1.03)  LDD1599  [8]
 LDCM0397  CL126 HEK-293T C1304(0.79)  LDD1601  [8]
 LDCM0399  CL128 HEK-293T C1304(1.29); C508(1.26)  LDD1603  [8]
 LDCM0401  CL14 HEK-293T C1304(0.92)  LDD1605  [8]
 LDCM0403  CL16 HEK-293T C1304(1.14); C508(1.16)  LDD1607  [8]
 LDCM0405  CL18 HEK-293T C508(1.08)  LDD1609  [8]
 LDCM0406  CL19 HEK-293T C508(0.82); C1104(1.07)  LDD1610  [8]
 LDCM0407  CL2 HEK-293T C1304(0.81)  LDD1611  [8]
 LDCM0408  CL20 HEK-293T C1068(1.04)  LDD1612  [8]
 LDCM0410  CL22 HEK-293T C560(0.85)  LDD1614  [8]
 LDCM0411  CL23 HEK-293T C508(0.85)  LDD1615  [8]
 LDCM0414  CL26 HEK-293T C1304(0.80)  LDD1618  [8]
 LDCM0416  CL28 HEK-293T C1304(1.00); C508(1.33)  LDD1620  [8]
 LDCM0419  CL30 HEK-293T C508(0.87)  LDD1623  [8]
 LDCM0420  CL31 HEK-293T C508(0.97); C1104(1.10)  LDD1624  [8]
 LDCM0421  CL32 HEK-293T C1068(1.13)  LDD1625  [8]
 LDCM0423  CL34 HEK-293T C560(0.95)  LDD1627  [8]
 LDCM0424  CL35 HEK-293T C508(1.02)  LDD1628  [8]
 LDCM0429  CL4 HEK-293T C1304(1.11); C508(0.97)  LDD1633  [8]
 LDCM0430  CL40 HEK-293T C1304(1.21); C508(1.16)  LDD1634  [8]
 LDCM0432  CL42 HEK-293T C508(1.04)  LDD1636  [8]
 LDCM0433  CL43 HEK-293T C508(0.79); C1104(0.94)  LDD1637  [8]
 LDCM0434  CL44 HEK-293T C1068(1.63)  LDD1638  [8]
 LDCM0436  CL46 HEK-293T C560(0.90)  LDD1640  [8]
 LDCM0437  CL47 HEK-293T C508(1.08)  LDD1641  [8]
 LDCM0441  CL50 HEK-293T C1304(1.04)  LDD1645  [8]
 LDCM0443  CL52 HEK-293T C1304(1.05); C508(1.08)  LDD1646  [8]
 LDCM0445  CL54 HEK-293T C508(0.98)  LDD1648  [8]
 LDCM0446  CL55 HEK-293T C508(1.00); C1104(1.19)  LDD1649  [8]
 LDCM0447  CL56 HEK-293T C1068(1.32)  LDD1650  [8]
 LDCM0449  CL58 HEK-293T C560(1.33)  LDD1652  [8]
 LDCM0450  CL59 HEK-293T C508(0.96)  LDD1653  [8]
 LDCM0451  CL6 HEK-293T C508(1.27)  LDD1654  [8]
 LDCM0454  CL62 HEK-293T C1304(0.82)  LDD1657  [8]
 LDCM0456  CL64 HEK-293T C1304(0.97); C508(1.02)  LDD1659  [8]
 LDCM0458  CL66 HEK-293T C508(1.10)  LDD1661  [8]
 LDCM0459  CL67 HEK-293T C508(0.94); C1104(1.04)  LDD1662  [8]
 LDCM0460  CL68 HEK-293T C1068(1.14)  LDD1663  [8]
 LDCM0462  CL7 HEK-293T C508(0.89); C1104(1.10)  LDD1665  [8]
 LDCM0463  CL70 HEK-293T C560(0.99)  LDD1666  [8]
 LDCM0464  CL71 HEK-293T C508(1.02)  LDD1667  [8]
 LDCM0467  CL74 HEK-293T C1304(0.75)  LDD1670  [8]
 LDCM0469  CL76 HEK-293T C1304(1.03); C508(0.96)  LDD1672  [8]
 LDCM0471  CL78 HEK-293T C508(1.03)  LDD1674  [8]
 LDCM0472  CL79 HEK-293T C508(0.75); C1104(1.00)  LDD1675  [8]
 LDCM0473  CL8 HEK-293T C1068(1.19)  LDD1676  [8]
 LDCM0474  CL80 HEK-293T C1068(1.00)  LDD1677  [8]
 LDCM0476  CL82 HEK-293T C560(0.89)  LDD1679  [8]
 LDCM0477  CL83 HEK-293T C508(0.92)  LDD1680  [8]
 LDCM0480  CL86 HEK-293T C1304(0.75)  LDD1683  [8]
 LDCM0482  CL88 HEK-293T C1304(1.00); C508(0.98)  LDD1685  [8]
 LDCM0485  CL90 HEK-293T C508(0.89)  LDD1688  [8]
 LDCM0486  CL91 HEK-293T C508(0.96); C1104(1.11)  LDD1689  [8]
 LDCM0487  CL92 HEK-293T C1068(1.13)  LDD1690  [8]
 LDCM0489  CL94 HEK-293T C560(0.97)  LDD1692  [8]
 LDCM0490  CL95 HEK-293T C508(0.98)  LDD1693  [8]
 LDCM0493  CL98 HEK-293T C1304(0.74)  LDD1696  [8]
 LDCM0427  Fragment51 HEK-293T C1304(0.86)  LDD1631  [8]
 LDCM0022  KB02 HEK-293T C863(0.79)  LDD1492  [8]
 LDCM0023  KB03 HEK-293T C863(1.16)  LDD1497  [8]
 LDCM0024  KB05 MSTO-211H C1403(2.14); C1104(2.27)  LDD3337  [3]

References

1 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
6 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
7 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
8 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402