Details of the Target
General Information of Target
| Target ID | LDTP09399 | |||||
|---|---|---|---|---|---|---|
| Target Name | Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (PREX1) | |||||
| Gene Name | PREX1 | |||||
| Gene ID | 57580 | |||||
| Synonyms |
KIAA1415; Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein; P-Rex1; PtdIns(3,4,5)-dependent Rac exchanger 1 |
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| 3D Structure | ||||||
| Sequence |
MEAPSGSEPGGDGAGDCAHPDPRAPGAAAPSSGPGPCAAARESERQLRLRLCVLNEILGT
ERDYVGTLRFLQSAFLHRIRQNVADSVEKGLTEENVKVLFSNIEDILEVHKDFLAALEYC LHPEPQSQHELGNVFLKFKDKFCVYEEYCSNHEKALRLLVELNKIPTVRAFLLSCMLLGG RKTTDIPLEGYLLSPIQRICKYPLLLKELAKRTPGKHPDHPAVQSALQAMKTVCSNINET KRQMEKLEALEQLQSHIEGWEGSNLTDICTQLLLQGTLLKISAGNIQERAFFLFDNLLVY CKRKSRVTGSKKSTKRTKSINGSLYIFRGRINTEVMEVENVEDGTADYHSNGYTVTNGWK IHNTAKNKWFVCMAKTAEEKQKWLDAIIREREQRESLKLGMERDAYVMIAEKGEKLYHMM MNKKVNLIKDRRRKLSTVPKCFLGNEFVAWLLEIGEISKTEEGVNLGQALLENGIIHHVS DKHQFKNEQVMYRFRYDDGTYKARSELEDIMSKGVRLYCRLHSLYTPVIKDRDYHLKTYK SVLPGSKLVDWLLAQGDCQTREEAVALGVGLCNNGFMHHVLEKSEFRDESQYFRFHADEE MEGTSSKNKQLRNDFKLVENILAKRLLILPQEEDYGFDIEEKNKAVVVKSVQRGSLAEVA GLQVGRKIYSINEDLVFLRPFSEVESILNQSFCSRRPLRLLVATKAKEIIKIPDQPDTLC FQIRGAAPPYVYAVGRGSEAMAAGLCAGQCILKVNGSNVMNDGAPEVLEHFQAFRSRREE ALGLYQWIYHTHEDAQEARASQEASTEDPSGEQAQEEDQADSAFPLLSLGPRLSLCEDSP MVTLTVDNVHLEHGVVYEYVSTAGVRCHVLEKIVEPRGCFGLTAKILEAFAANDSVFVEN CRRLMALSSAIVTMPHFEFRNICDTKLESIGQRIACYQEFAAQLKSRVSPPFKQAPLEPH PLCGLDFCPTNCHINLMEVSYPKTTPSVGRSFSIRFGRKPSLIGLDPEQGHLNPMSYTQH CITTMAAPSWKCLPAAEGDPQGQGLHDGSFGPASGTLGQEDRGLSFLLKQEDREIQDAYL QLFTKLDVALKEMKQYVTQINRLLSTITEPTSGGSCDASLAEEASSLPLVSEESEMDRSD HGGIKKVCFKVAEEDQEDSGHDTMSYRDSYSECNSNRDSVLSYTSVRSNSSYLGSDEMGS GDELPCDMRIPSDKQDKLHGCLEHLFNQVDSINALLKGPVMSRAFEETKHFPMNHSLQEF KQKEECTIRGRSLIQISIQEDPWNLPNSIKTLVDNIQRYVEDGKNQLLLALLKCTDTELQ LRRDAIFCQALVAAVCTFSKQLLAALGYRYNNNGEYEESSRDASRKWLEQVAATGVLLHC QSLLSPATVKEERTMLEDIWVTLSELDNVTFSFKQLDENYVANTNVFYHIEGSRQALKVI FYLDSYHFSKLPSRLEGGASLRLHTALFTKVLENVEGLPSPGSQAAEDLQQDINAQSLEK VQQYYRKLRAFYLERSNLPTDASTTAVKIDQLIRPINALDELCRLMKSFVHPKPGAAGSV GAGLIPISSELCYRLGACQMVMCGTGMQRSTLSVSLEQAAILARSHGLLPKCIMQATDIM RKQGPRVEILAKNLRVKDQMPQGAPRLYRLCQPPVDGDL |
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| Target Bioclass |
Other
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| Subcellular location |
Cytoplasm, cytosol
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| Function |
Functions as a RAC guanine nucleotide exchange factor (GEF), which activates the Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. May function downstream of heterotrimeric G proteins in neutrophils.
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| Uniprot ID | ||||||
| Ensemble ID | ||||||
| HGNC ID | ||||||
Target Site Mutations in Different Cell Lines
| Cell line | Mutation details | Probe for labeling this protein in this cell | |||
|---|---|---|---|---|---|
| A3KAW | Deletion: p.A29PfsTer105 | . | |||
| AU565 | SNV: p.N643K | DBIA Probe Info | |||
| HUH28 | SNV: p.A115T | . | |||
| IGR1 | SNV: p.D717N | DBIA Probe Info | |||
| IGROV1 | SNV: p.H578Y; p.V1229A; p.R1534C | DBIA Probe Info | |||
| KMRC3 | SNV: p.W787C | . | |||
| LS180 | SNV: p.I911V; p.A1526T | . | |||
| MELJUSO | SNV: p.P986T | DBIA Probe Info | |||
| MFE319 | SNV: p.S1569P | . | |||
| MOLT4 | SNV: p.A1616V | . | |||
| NALM6 | SNV: p.D1207V | DBIA Probe Info | |||
| NCIH1155 | SNV: p.S458N | . | |||
| NCIH2172 | SNV: p.R990M | . | |||
| PC14 | SNV: p.E1135D | . | |||
| PC9 | SNV: p.E1135D | . | |||
| PF382 | SNV: p.R995H | DBIA Probe Info | |||
| SNU245 | SNV: p.R1515L | . | |||
| SNU308 | SNV: p.R778P | . | |||
| SW1271 | SNV: p.D717Y | . | |||
| SW480 | SNV: p.L1193F | . | |||
| TYKNU | SNV: p.S1242T | . | |||
Probe(s) Labeling This Target
ABPP Probe
| Probe name | Structure | Binding Site(Ratio) | Interaction ID | Ref | |
|---|---|---|---|---|---|
|
Probe 1 Probe Info |
 |
Y406(18.21); Y492(36.99); Y501(15.24); Y592(25.01) | LDD3495 | [1] | |
|
DBIA Probe Info |
 |
C37(2.57); C936(1.27) | LDD3332 | [2] | |
|
AHL-Pu-1 Probe Info |
 |
C52(4.21) | LDD0171 | [3] | |
|
IA-alkyne Probe Info |
 |
C1266(9.45) | LDD0516 | [4] | |
|
N1 Probe Info |
 |
D1302(0.00); Y1299(0.00) | LDD0245 | [5] | |
|
Lodoacetamide azide Probe Info |
 |
N.A. | LDD0037 | [6] | |
|
IPM Probe Info |
 |
N.A. | LDD2156 | [7] | |
|
NAIA_5 Probe Info |
 |
C301(0.00); C175(0.00); C720(0.00); C441(0.00) | LDD2223 | [8] | |
Competitor(s) Related to This Target
| Competitor ID | Name | Cell line | Binding Site(Ratio) | Interaction ID | Ref |
|---|---|---|---|---|---|
| LDCM0026 | 4SU-RNA+native RNA | DM93 | C52(4.21) | LDD0171 | [3] |
| LDCM0200 | BPK-25 | T cell | C1266(9.45) | LDD0516 | [4] |
| LDCM0632 | CL-Sc | Hep-G2 | C1612(20.00); C1173(20.00); C746(20.00); C1543(2.61) | LDD2227 | [8] |
| LDCM0634 | CY-0357 | Hep-G2 | C1173(2.09); C17(0.93); C37(0.87); C1206(0.50) | LDD2228 | [8] |
| LDCM0203 | EV-96 | T cell | C1266(4.34) | LDD0523 | [4] |
| LDCM0204 | EV-97 | T cell | C1266(16.45) | LDD0524 | [4] |
| LDCM0022 | KB02 | T cell | C37(5.76); C1266(20.00) | LDD1703 | [4] |
| LDCM0023 | KB03 | 42-MG-BA | C37(2.22); C879(2.19) | LDD2661 | [2] |
| LDCM0024 | KB05 | MKN45 | C37(2.57); C936(1.27) | LDD3332 | [2] |
The Interaction Atlas With This Target
The Protein(s) Related To This Target
Enzyme
| Protein name | Family | Uniprot ID | |||
|---|---|---|---|---|---|
| Serine/threonine-protein kinase mTOR (MTOR) | PI3/PI4-kinase family | P42345 | |||
Transporter and channel
| Protein name | Family | Uniprot ID | |||
|---|---|---|---|---|---|
| Rapamycin-insensitive companion of mTOR (RICTOR) | RICTOR family | Q6R327 | |||
Other
| Protein name | Family | Uniprot ID | |||
|---|---|---|---|---|---|
| Regulatory-associated protein of mTOR (RPTOR) | WD repeat RAPTOR family | Q8N122 | |||
References