Details of the Target

General Information of Target

Target ID LDTP09223
Target Name Inositol polyphosphate multikinase (IPMK)
Gene Name IPMK
Gene ID 253430
Synonyms
IMPK; Inositol polyphosphate multikinase; EC 2.7.1.140; EC 2.7.1.151; EC 2.7.1.153; Inositol 1,3,4,6-tetrakisphosphate 5-kinase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MATEPPSPLRVEAPGPPEMRTSPAIESTPEGTPQPAGGRLRFLNGCVPLSHQVAGHMYGK
DKVGILQHPDGTVLKQLQPPPRGPRELEFYNMVYAADCFDGVLLELRKYLPKYYGIWSPP
TAPNDLYLKLEDVTHKFNKPCIMDVKIGQKSYDPFASSEKIQQQVSKYPLMEEIGFLVLG
MRVYHVHSDSYETENQHYGRSLTKETIKDGVSRFFHNGYCLRKDAVAASIQKIEKILQWF
ENQKQLNFYASSLLFVYEGSSQPTTTKLNDRTLAEKFLSKGQLSDTEVLEYNNNFHVLSS
TANGKIESSVGKSLSKMYARHRKIYTKKHHSQTSLKVENLEQDNGWKSMSQEHLNGNVLS
QLEKVFYHLPTGCQEIAEVEVRMIDFAHVFPSNTIDEGYVYGLKHLISVLRSILDN
Target Bioclass
Enzyme
Family
Inositol phosphokinase (IPK) family
Subcellular location
Nucleus
Function
Inositol phosphate kinase with a broad substrate specificity. Phosphorylates inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) first to inositol 1,3,4,5-tetrakisphosphate and then to inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5). Phosphorylates inositol 1,3,4,6-tetrakisphosphate (Ins(1,3,4,6)P4). Phosphorylates inositol 1,4,5,6-tetrakisphosphate (Ins(1,4,5,6)P4). Phosphorylates glycero-3-phospho-1D-myo-inositol 4,5-bisphosphate to glycero-3-phospho-1D-myo-inositol 3,4,5-trisphosphate. Plays an important role in MLKL-mediated necroptosis via its role in the biosynthesis of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6). Binding of these highly phosphorylated inositol phosphates to MLKL mediates the release of an N-terminal auto-inhibitory region, leading to activation of the kinase. Essential for activated phospho-MLKL to oligomerize and localize to the cell membrane during necroptosis. Required for normal embryonic development, probably via its role in the biosynthesis of inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5) and inositol hexakisphosphate (InsP6).
Uniprot ID
Q8NFU5
Ensemble ID
ENST00000373935.4
HGNC ID
HGNC:20739
ChEMBL ID
CHEMBL4523401

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C220(1.09)  LDD0080  [1]
ATP probe
 Probe Info  		N.A.  LDD0199  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0226  AC11 HEK-293T C373(1.27)  LDD1509  [3]
 LDCM0237  AC12 HEK-293T C373(1.06)  LDD1510  [3]
 LDCM0259  AC14 HEK-293T C373(1.15)  LDD1512  [3]
 LDCM0278  AC19 HEK-293T C373(1.51)  LDD1517  [3]
 LDCM0280  AC20 HEK-293T C373(0.84)  LDD1519  [3]
 LDCM0281  AC21 HEK-293T C373(0.81)  LDD1520  [3]
 LDCM0282  AC22 HEK-293T C373(0.75)  LDD1521  [3]
 LDCM0284  AC24 HEK-293T C373(0.81)  LDD1523  [3]
 LDCM0287  AC27 HEK-293T C373(1.12)  LDD1526  [3]
 LDCM0288  AC28 HEK-293T C373(0.94)  LDD1527  [3]
 LDCM0289  AC29 HEK-293T C373(0.97)  LDD1528  [3]
 LDCM0290  AC3 HEK-293T C373(1.23)  LDD1529  [3]
 LDCM0291  AC30 HEK-293T C373(1.06)  LDD1530  [3]
 LDCM0293  AC32 HEK-293T C373(0.76)  LDD1532  [3]
 LDCM0296  AC35 HEK-293T C373(0.93)  LDD1535  [3]
 LDCM0297  AC36 HEK-293T C373(0.92)  LDD1536  [3]
 LDCM0298  AC37 HEK-293T C373(1.07)  LDD1537  [3]
 LDCM0299  AC38 HEK-293T C373(1.02)  LDD1538  [3]
 LDCM0301  AC4 HEK-293T C373(0.82)  LDD1540  [3]
 LDCM0302  AC40 HEK-293T C373(0.83)  LDD1541  [3]
 LDCM0305  AC43 HEK-293T C373(1.05)  LDD1544  [3]
 LDCM0306  AC44 HEK-293T C373(1.18)  LDD1545  [3]
 LDCM0307  AC45 HEK-293T C373(1.13)  LDD1546  [3]
 LDCM0308  AC46 HEK-293T C373(1.10)  LDD1547  [3]
 LDCM0310  AC48 HEK-293T C373(0.93)  LDD1549  [3]
 LDCM0312  AC5 HEK-293T C373(1.01)  LDD1551  [3]
 LDCM0314  AC51 HEK-293T C373(1.22)  LDD1553  [3]
 LDCM0315  AC52 HEK-293T C373(0.99)  LDD1554  [3]
 LDCM0316  AC53 HEK-293T C373(0.90)  LDD1555  [3]
 LDCM0317  AC54 HEK-293T C373(1.04)  LDD1556  [3]
 LDCM0319  AC56 HEK-293T C373(1.13)  LDD1558  [3]
 LDCM0322  AC59 HEK-293T C373(1.25)  LDD1561  [3]
 LDCM0323  AC6 HEK-293T C373(0.94)  LDD1562  [3]
 LDCM0324  AC60 HEK-293T C373(0.93)  LDD1563  [3]
 LDCM0325  AC61 HEK-293T C373(1.13)  LDD1564  [3]
 LDCM0326  AC62 HEK-293T C373(1.31)  LDD1565  [3]
 LDCM0328  AC64 HEK-293T C373(0.92)  LDD1567  [3]
 LDCM0345  AC8 HEK-293T C373(0.81)  LDD1569  [3]
 LDCM0248  AKOS034007472 HEK-293T C373(1.21)  LDD1511  [3]
 LDCM0275  AKOS034007705 HEK-293T C373(1.32)  LDD1514  [3]
 LDCM0368  CL10 HEK-293T C373(1.05)  LDD1572  [3]
 LDCM0371  CL102 HEK-293T C373(0.70)  LDD1575  [3]
 LDCM0372  CL103 HEK-293T C373(1.31)  LDD1576  [3]
 LDCM0375  CL106 HEK-293T C373(1.10)  LDD1579  [3]
 LDCM0376  CL107 HEK-293T C373(1.45)  LDD1580  [3]
 LDCM0380  CL110 HEK-293T C373(1.13)  LDD1584  [3]
 LDCM0381  CL111 HEK-293T C373(1.11)  LDD1585  [3]
 LDCM0384  CL114 HEK-293T C373(1.03)  LDD1588  [3]
 LDCM0385  CL115 HEK-293T C373(1.10)  LDD1589  [3]
 LDCM0388  CL118 HEK-293T C373(0.87)  LDD1592  [3]
 LDCM0389  CL119 HEK-293T C373(1.48)  LDD1593  [3]
 LDCM0390  CL12 HEK-293T C373(0.87)  LDD1594  [3]
 LDCM0393  CL122 HEK-293T C373(0.75)  LDD1597  [3]
 LDCM0394  CL123 HEK-293T C373(1.27)  LDD1598  [3]
 LDCM0397  CL126 HEK-293T C373(1.05)  LDD1601  [3]
 LDCM0398  CL127 HEK-293T C373(2.04)  LDD1602  [3]
 LDCM0401  CL14 HEK-293T C373(0.56)  LDD1605  [3]
 LDCM0402  CL15 HEK-293T C373(0.81)  LDD1606  [3]
 LDCM0406  CL19 HEK-293T C373(1.07)  LDD1610  [3]
 LDCM0407  CL2 HEK-293T C373(0.84)  LDD1611  [3]
 LDCM0408  CL20 HEK-293T C373(0.83)  LDD1612  [3]
 LDCM0409  CL21 HEK-293T C373(0.92)  LDD1613  [3]
 LDCM0410  CL22 HEK-293T C373(1.54)  LDD1614  [3]
 LDCM0412  CL24 HEK-293T C373(1.01)  LDD1616  [3]
 LDCM0414  CL26 HEK-293T C373(1.00)  LDD1618  [3]
 LDCM0415  CL27 HEK-293T C373(1.10)  LDD1619  [3]
 LDCM0418  CL3 HEK-293T C373(1.34)  LDD1622  [3]
 LDCM0420  CL31 HEK-293T C373(0.78)  LDD1624  [3]
 LDCM0421  CL32 HEK-293T C373(0.87)  LDD1625  [3]
 LDCM0422  CL33 HEK-293T C373(0.89)  LDD1626  [3]
 LDCM0423  CL34 HEK-293T C373(1.00)  LDD1627  [3]
 LDCM0425  CL36 HEK-293T C373(0.81)  LDD1629  [3]
 LDCM0428  CL39 HEK-293T C373(0.55)  LDD1632  [3]
 LDCM0433  CL43 HEK-293T C373(1.16)  LDD1637  [3]
 LDCM0434  CL44 HEK-293T C373(1.00)  LDD1638  [3]
 LDCM0435  CL45 HEK-293T C373(0.80)  LDD1639  [3]
 LDCM0436  CL46 HEK-293T C373(1.03)  LDD1640  [3]
 LDCM0438  CL48 HEK-293T C373(0.68)  LDD1642  [3]
 LDCM0441  CL50 HEK-293T C373(0.85)  LDD1645  [3]
 LDCM0446  CL55 HEK-293T C373(1.33)  LDD1649  [3]
 LDCM0447  CL56 HEK-293T C373(1.02)  LDD1650  [3]
 LDCM0448  CL57 HEK-293T C373(0.89)  LDD1651  [3]
 LDCM0449  CL58 HEK-293T C373(0.92)  LDD1652  [3]
 LDCM0452  CL60 HEK-293T C373(1.10)  LDD1655  [3]
 LDCM0454  CL62 HEK-293T C373(0.97)  LDD1657  [3]
 LDCM0455  CL63 HEK-293T C373(1.30)  LDD1658  [3]
 LDCM0459  CL67 HEK-293T C373(1.04)  LDD1662  [3]
 LDCM0460  CL68 HEK-293T C373(0.98)  LDD1663  [3]
 LDCM0461  CL69 HEK-293T C373(1.12)  LDD1664  [3]
 LDCM0462  CL7 HEK-293T C373(1.09)  LDD1665  [3]
 LDCM0463  CL70 HEK-293T C373(1.24)  LDD1666  [3]
 LDCM0465  CL72 HEK-293T C373(1.17)  LDD1668  [3]
 LDCM0467  CL74 HEK-293T C373(1.02)  LDD1670  [3]
 LDCM0472  CL79 HEK-293T C373(0.97)  LDD1675  [3]
 LDCM0473  CL8 HEK-293T C373(1.26)  LDD1676  [3]
 LDCM0474  CL80 HEK-293T C373(0.95)  LDD1677  [3]
 LDCM0475  CL81 HEK-293T C373(1.16)  LDD1678  [3]
 LDCM0476  CL82 HEK-293T C373(0.90)  LDD1679  [3]
 LDCM0478  CL84 HEK-293T C373(1.29)  LDD1681  [3]
 LDCM0480  CL86 HEK-293T C373(0.57)  LDD1683  [3]
 LDCM0481  CL87 HEK-293T C373(0.83)  LDD1684  [3]
 LDCM0484  CL9 HEK-293T C373(1.31)  LDD1687  [3]
 LDCM0486  CL91 HEK-293T C373(0.96)  LDD1689  [3]
 LDCM0487  CL92 HEK-293T C373(1.08)  LDD1690  [3]
 LDCM0488  CL93 HEK-293T C373(0.75)  LDD1691  [3]
 LDCM0489  CL94 HEK-293T C373(0.95)  LDD1692  [3]
 LDCM0491  CL96 HEK-293T C373(0.89)  LDD1694  [3]
 LDCM0493  CL98 HEK-293T C373(0.95)  LDD1696  [3]
 LDCM0494  CL99 HEK-293T C373(1.48)  LDD1697  [3]
 LDCM0495  E2913 HEK-293T C373(1.13)  LDD1698  [3]
 LDCM0468  Fragment33 HEK-293T C373(1.39)  LDD1671  [3]
 LDCM0427  Fragment51 HEK-293T C373(0.69)  LDD1631  [3]
 LDCM0022  KB02 HCT 116 C220(1.09)  LDD0080  [1]
 LDCM0023  KB03 HCT 116 C220(1.12)  LDD0081  [1]
 LDCM0024  KB05 HCT 116 C220(1.39)  LDD0082  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Heat shock factor 2-binding protein (HSF2BP) . O75031

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402