Details of the Target

General Information of Target

Target ID LDTP09003
Target Name Probable E3 ubiquitin-protein ligase DTX3 (DTX3)
Gene Name DTX3
Gene ID 196403
Synonyms
RNF154; Probable E3 ubiquitin-protein ligase DTX3; EC 2.3.2.27; Protein deltex-3; Deltex3; RING finger protein 154; RING-type E3 ubiquitin transferase DTX3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSFVLSRMAACGGTCKNKVTVSKPVWDFLSKETPARLARLREEHRVSILIDGETSDIYVL
QLSPQGPPPAPPNGLYLARKALKGLLKEAEKELKKAQRQGELMGCLALGGGGEHPEMHRA
GPPPLRAAPLLPPGARGLPPPPPPLPPPLPPRLREEAEEQESTCPICLGEIQNAKTLEKC
RHSFCEGCITRALQVKKACPMCGRFYGQLVGNQPQNGRMLVSKDATLLLPSYEKYGTIVI
QYVFPPGVQGAEHPNPGVRYPGTTRVAYLPDCPEGNKVLTLFRKAFDQRLTFTIGTSMTT
GRPNVITWNDIHHKTSCTGGPQLFGYPDPTYLTRVQEELRAKGITDD
Target Bioclass
Enzyme
Family
Deltex family
Subcellular location
Cytoplasm
Function
Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.
Uniprot ID
Q8N9I9
Ensemble ID
ENST00000337737.8
HGNC ID
HGNC:24457

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
PF382 SNV: p.N276D .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K83(5.88)  LDD0277  [1]
DBIA
 Probe Info  		C275(2.66)  LDD3340  [2]
NAIA_5
 Probe Info  		C272(1.27)  LDD2227  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C272(1.41)  LDD1507  [4]
 LDCM0226  AC11 HEK-293T C272(1.07)  LDD1509  [4]
 LDCM0237  AC12 HEK-293T C272(1.23)  LDD1510  [4]
 LDCM0259  AC14 HEK-293T C272(1.21)  LDD1512  [4]
 LDCM0270  AC15 HEK-293T C272(1.08)  LDD1513  [4]
 LDCM0276  AC17 HEK-293T C272(0.91)  LDD1515  [4]
 LDCM0278  AC19 HEK-293T C272(0.92)  LDD1517  [4]
 LDCM0280  AC20 HEK-293T C272(1.22)  LDD1519  [4]
 LDCM0282  AC22 HEK-293T C272(1.14)  LDD1521  [4]
 LDCM0283  AC23 HEK-293T C272(0.93)  LDD1522  [4]
 LDCM0285  AC25 HEK-293T C272(1.11)  LDD1524  [4]
 LDCM0287  AC27 HEK-293T C272(0.92)  LDD1526  [4]
 LDCM0288  AC28 HEK-293T C272(1.27)  LDD1527  [4]
 LDCM0290  AC3 HEK-293T C272(1.05)  LDD1529  [4]
 LDCM0291  AC30 HEK-293T C272(1.20)  LDD1530  [4]
 LDCM0292  AC31 HEK-293T C272(0.99)  LDD1531  [4]
 LDCM0294  AC33 HEK-293T C272(0.89)  LDD1533  [4]
 LDCM0296  AC35 HEK-293T C272(1.07)  LDD1535  [4]
 LDCM0297  AC36 HEK-293T C272(1.03)  LDD1536  [4]
 LDCM0299  AC38 HEK-293T C272(1.28)  LDD1538  [4]
 LDCM0300  AC39 HEK-293T C272(1.00)  LDD1539  [4]
 LDCM0301  AC4 HEK-293T C272(1.16)  LDD1540  [4]
 LDCM0303  AC41 HEK-293T C272(1.35)  LDD1542  [4]
 LDCM0305  AC43 HEK-293T C272(1.09)  LDD1544  [4]
 LDCM0306  AC44 HEK-293T C272(1.12)  LDD1545  [4]
 LDCM0308  AC46 HEK-293T C272(0.92)  LDD1547  [4]
 LDCM0309  AC47 HEK-293T C272(1.01)  LDD1548  [4]
 LDCM0311  AC49 HEK-293T C272(1.62)  LDD1550  [4]
 LDCM0314  AC51 HEK-293T C272(1.04)  LDD1553  [4]
 LDCM0315  AC52 HEK-293T C272(1.52)  LDD1554  [4]
 LDCM0317  AC54 HEK-293T C272(1.06)  LDD1556  [4]
 LDCM0318  AC55 HEK-293T C272(0.94)  LDD1557  [4]
 LDCM0320  AC57 HEK-293T C272(1.03)  LDD1559  [4]
 LDCM0322  AC59 HEK-293T C272(1.09)  LDD1561  [4]
 LDCM0323  AC6 HEK-293T C272(1.37)  LDD1562  [4]
 LDCM0324  AC60 HEK-293T C272(1.25)  LDD1563  [4]
 LDCM0326  AC62 HEK-293T C272(1.35)  LDD1565  [4]
 LDCM0327  AC63 HEK-293T C272(1.26)  LDD1566  [4]
 LDCM0334  AC7 HEK-293T C272(0.97)  LDD1568  [4]
 LDCM0356  AKOS034007680 HEK-293T C272(1.18)  LDD1570  [4]
 LDCM0632  CL-Sc Hep-G2 C272(1.27)  LDD2227  [3]
 LDCM0368  CL10 HEK-293T C272(1.25)  LDD1572  [4]
 LDCM0369  CL100 HEK-293T C272(0.91)  LDD1573  [4]
 LDCM0371  CL102 HEK-293T C272(1.03)  LDD1575  [4]
 LDCM0373  CL104 HEK-293T C272(1.13)  LDD1577  [4]
 LDCM0375  CL106 HEK-293T C272(1.01)  LDD1579  [4]
 LDCM0377  CL108 HEK-293T C272(1.14)  LDD1581  [4]
 LDCM0379  CL11 HEK-293T C272(1.16)  LDD1583  [4]
 LDCM0380  CL110 HEK-293T C272(1.08)  LDD1584  [4]
 LDCM0382  CL112 HEK-293T C272(0.99)  LDD1586  [4]
 LDCM0384  CL114 HEK-293T C272(1.05)  LDD1588  [4]
 LDCM0386  CL116 HEK-293T C272(0.90)  LDD1590  [4]
 LDCM0388  CL118 HEK-293T C272(1.02)  LDD1592  [4]
 LDCM0391  CL120 HEK-293T C272(0.77)  LDD1595  [4]
 LDCM0393  CL122 HEK-293T C272(1.02)  LDD1597  [4]
 LDCM0395  CL124 HEK-293T C272(0.95)  LDD1599  [4]
 LDCM0397  CL126 HEK-293T C272(1.20)  LDD1601  [4]
 LDCM0399  CL128 HEK-293T C272(1.37)  LDD1603  [4]
 LDCM0401  CL14 HEK-293T C272(1.10)  LDD1605  [4]
 LDCM0403  CL16 HEK-293T C272(1.05)  LDD1607  [4]
 LDCM0404  CL17 HEK-293T C272(1.36)  LDD1608  [4]
 LDCM0406  CL19 HEK-293T C272(1.08)  LDD1610  [4]
 LDCM0407  CL2 HEK-293T C272(1.11)  LDD1611  [4]
 LDCM0408  CL20 HEK-293T C272(1.29)  LDD1612  [4]
 LDCM0410  CL22 HEK-293T C272(1.39)  LDD1614  [4]
 LDCM0411  CL23 HEK-293T C272(1.19)  LDD1615  [4]
 LDCM0414  CL26 HEK-293T C272(1.05)  LDD1618  [4]
 LDCM0416  CL28 HEK-293T C272(1.28)  LDD1620  [4]
 LDCM0417  CL29 HEK-293T C272(1.06)  LDD1621  [4]
 LDCM0420  CL31 HEK-293T C272(1.24)  LDD1624  [4]
 LDCM0421  CL32 HEK-293T C272(1.27)  LDD1625  [4]
 LDCM0423  CL34 HEK-293T C272(1.04)  LDD1627  [4]
 LDCM0424  CL35 HEK-293T C272(1.11)  LDD1628  [4]
 LDCM0429  CL4 HEK-293T C272(0.99)  LDD1633  [4]
 LDCM0430  CL40 HEK-293T C272(0.98)  LDD1634  [4]
 LDCM0431  CL41 HEK-293T C272(1.29)  LDD1635  [4]
 LDCM0433  CL43 HEK-293T C272(1.09)  LDD1637  [4]
 LDCM0434  CL44 HEK-293T C272(1.19)  LDD1638  [4]
 LDCM0436  CL46 HEK-293T C272(1.05)  LDD1640  [4]
 LDCM0437  CL47 HEK-293T C272(1.09)  LDD1641  [4]
 LDCM0440  CL5 HEK-293T C272(1.28)  LDD1644  [4]
 LDCM0441  CL50 HEK-293T C272(1.08)  LDD1645  [4]
 LDCM0443  CL52 HEK-293T C272(0.80)  LDD1646  [4]
 LDCM0444  CL53 HEK-293T C272(1.10)  LDD1647  [4]
 LDCM0446  CL55 HEK-293T C272(1.15)  LDD1649  [4]
 LDCM0447  CL56 HEK-293T C272(1.37)  LDD1650  [4]
 LDCM0449  CL58 HEK-293T C272(1.95)  LDD1652  [4]
 LDCM0450  CL59 HEK-293T C272(1.14)  LDD1653  [4]
 LDCM0454  CL62 HEK-293T C272(1.02)  LDD1657  [4]
 LDCM0456  CL64 HEK-293T C272(0.93)  LDD1659  [4]
 LDCM0457  CL65 HEK-293T C272(0.93)  LDD1660  [4]
 LDCM0459  CL67 HEK-293T C272(1.12)  LDD1662  [4]
 LDCM0460  CL68 HEK-293T C272(1.07)  LDD1663  [4]
 LDCM0462  CL7 HEK-293T C272(1.08)  LDD1665  [4]
 LDCM0463  CL70 HEK-293T C272(0.85)  LDD1666  [4]
 LDCM0464  CL71 HEK-293T C272(1.23)  LDD1667  [4]
 LDCM0467  CL74 HEK-293T C272(1.02)  LDD1670  [4]
 LDCM0469  CL76 HEK-293T C272(1.43)  LDD1672  [4]
 LDCM0470  CL77 HEK-293T C272(1.23)  LDD1673  [4]
 LDCM0472  CL79 HEK-293T C272(1.02)  LDD1675  [4]
 LDCM0473  CL8 HEK-293T C272(1.67)  LDD1676  [4]
 LDCM0474  CL80 HEK-293T C272(1.30)  LDD1677  [4]
 LDCM0476  CL82 HEK-293T C272(0.93)  LDD1679  [4]
 LDCM0477  CL83 HEK-293T C272(1.07)  LDD1680  [4]
 LDCM0480  CL86 HEK-293T C272(1.08)  LDD1683  [4]
 LDCM0482  CL88 HEK-293T C272(0.88)  LDD1685  [4]
 LDCM0483  CL89 HEK-293T C272(0.94)  LDD1686  [4]
 LDCM0486  CL91 HEK-293T C272(1.10)  LDD1689  [4]
 LDCM0487  CL92 HEK-293T C272(1.13)  LDD1690  [4]
 LDCM0489  CL94 HEK-293T C272(1.49)  LDD1692  [4]
 LDCM0490  CL95 HEK-293T C272(1.01)  LDD1693  [4]
 LDCM0493  CL98 HEK-293T C272(0.99)  LDD1696  [4]
 LDCM0427  Fragment51 HEK-293T C272(1.06)  LDD1631  [4]
 LDCM0022  KB02 A673 C275(3.67)  LDD2261  [2]
 LDCM0023  KB03 A673 C275(2.16)  LDD2678  [2]
 LDCM0024  KB05 NB1 C275(2.66)  LDD3340  [2]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402