Details of the Target

General Information of Target

Target ID LDTP08898
Target Name SH2 domain-containing protein 3C (SH2D3C)
Gene Name SH2D3C
Gene ID 10044
Synonyms
NSP3; SH2 domain-containing protein 3C; Cas/HEF1-associated signal transducer; Chat-H; Novel SH2-containing protein 3; SH2 domain-containing Eph receptor-binding protein 1; SHEP1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTEGTKKTSKKFKFFKFKGFGSLSNLPRSFTLRRSSASISRQSHLEPDTFEATQDDMVTV
PKSPPAYARSSDMYSHMGTMPRPSIKKAQNSQAARQAQEAGPKPNLVPGGVPDPPGLEAA
KEVMVKATGPLEDTPAMEPNPSAVEVDPIRKPEVPTGDVEEERPPRDVHSERAAGEPEAG
SDYVKFSKEKYILDSSPEKLHKELEEELKLSSTDLRSHAWYHGRIPREVSETLVQRNGDF
LIRDSLTSLGDYVLTCRWRNQALHFKINKVVVKAGESYTHIQYLFEQESFDHVPALVRYH
VGSRKAVSEQSGAIIYCPVNRTFPLRYLEASYGLGQGSSKPASPVSPSGPKGSHMKRRSV
TMTDGLTADKVTRSDGCPTSTSLPRPRDSIRSCALSMDQIPDLHSPMSPISESPSSPAYS
TVTRVHAAPAAPSATALPASPVARRSSEPQLCPGSAPKTHGESDKGPHTSPSHTLGKASP
SPSLSSYSDPDSGHYCQLQPPVRGSREWAATETSSQQARSYGERLKELSENGAPEGDWGK
TFTVPIVEVTSSFNPATFQSLLIPRDNRPLEVGLLRKVKELLAEVDARTLARHVTKVDCL
VARILGVTKEMQTLMGVRWGMELLTLPHGRQLRLDLLERFHTMSIMLAVDILGCTGSAEE
RAALLHKTIQLAAELRGTMGNMFSFAAVMGALDMAQISRLEQTWVTLRQRHTEGAILYEK
KLKPFLKSLNEGKEGPPLSNTTFPHVLPLITLLECDSAPPEGPEPWGSTEHGVEVVLAHL
EAARTVAHHGGLYHTNAEVKLQGFQARPELLEVFSTEFQMRLLWGSQGASSSQARRYEKF
DKVLTALSHKLEPAVRSSEL
Target Bioclass
Other
Subcellular location
Cytoplasm; Cell membrane
Function
Acts as an adapter protein that mediates cell signaling pathways involved in cellular functions such as cell adhesion and migration, tissue organization, and the regulation of the immune response. Plays a role in integrin-mediated cell adhesion through BCAR1-CRK-RAPGEF1 signaling and activation of the small GTPase RAP1. Promotes cell migration and invasion through the extracellular matrix. Required for marginal zone B-cell development and thymus-independent type 2 immune responses. Mediates migration and adhesion of B cells in the splenic marginal zone via promoting hyperphosphorylation of NEDD9/CASL. Plays a role in CXCL13-induced chemotaxis of B-cells. Plays a role in the migration of olfactory sensory neurons (OSNs) into the forebrain and the innervation of the olfactory bulb by the OSN axons during development. Required for the efficient tyrosine phosphorylation of BCAR1 in OSN axons.; [Isoform 1]: Important regulator of chemokine-induced, integrin-mediated T lymphocyte adhesion and migration, acting upstream of RAP1. Required for tissue-specific adhesion of T lymphocytes to peripheral tissues. Required for basal and CXCL2 stimulated serine-threonine phosphorylation of NEDD9. May be involved in the regulation of T-cell receptor-mediated IL2 production through the activation of the JNK pathway in T-cells.; [Isoform 2]: May be involved in the BCAR1/CAS-mediated JNK activation pathway.
Uniprot ID
Q8N5H7
Ensemble ID
ENST00000314830.13
HGNC ID
HGNC:16884

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HT1376 Substitution: p.A663T .
IM95 SNV: p.R592L .
JURKAT SNV: p.A686V .
JURLMK1 SNV: p.D182Y .
MCC13 SNV: p.E548K
Substitution: p.P27S		 .
MDST8 SNV: p.R150K .
OPM2 SNV: p.V607I .
REH SNV: p.A427V .
RKO SNV: p.A439T .
SUIT2 SNV: p.E527K .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C317(1.91)  LDD3370  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C599(0.98)  LDD1507  [2]
 LDCM0215  AC10 HEK-293T C452(0.97)  LDD1508  [2]
 LDCM0226  AC11 HEK-293T C317(0.96)  LDD1509  [2]
 LDCM0237  AC12 HEK-293T C452(1.02)  LDD1510  [2]
 LDCM0259  AC14 HEK-293T C452(1.09)  LDD1512  [2]
 LDCM0270  AC15 HEK-293T C393(1.01)  LDD1513  [2]
 LDCM0276  AC17 HEK-293T C599(1.07)  LDD1515  [2]
 LDCM0277  AC18 HEK-293T C452(0.96)  LDD1516  [2]
 LDCM0278  AC19 HEK-293T C317(1.27)  LDD1517  [2]
 LDCM0279  AC2 HEK-293T C452(1.04)  LDD1518  [2]
 LDCM0280  AC20 HEK-293T C452(1.09)  LDD1519  [2]
 LDCM0282  AC22 HEK-293T C452(1.10)  LDD1521  [2]
 LDCM0283  AC23 HEK-293T C393(1.05)  LDD1522  [2]
 LDCM0285  AC25 HEK-293T C599(1.05)  LDD1524  [2]
 LDCM0286  AC26 HEK-293T C452(1.09)  LDD1525  [2]
 LDCM0287  AC27 HEK-293T C317(1.11)  LDD1526  [2]
 LDCM0288  AC28 HEK-293T C452(1.13)  LDD1527  [2]
 LDCM0290  AC3 HEK-293T C317(1.17)  LDD1529  [2]
 LDCM0291  AC30 HEK-293T C452(0.95)  LDD1530  [2]
 LDCM0292  AC31 HEK-293T C393(1.08)  LDD1531  [2]
 LDCM0294  AC33 HEK-293T C599(1.06)  LDD1533  [2]
 LDCM0295  AC34 HEK-293T C452(1.29)  LDD1534  [2]
 LDCM0296  AC35 HEK-293T C317(1.04)  LDD1535  [2]
 LDCM0297  AC36 HEK-293T C452(1.05)  LDD1536  [2]
 LDCM0299  AC38 HEK-293T C452(0.98)  LDD1538  [2]
 LDCM0300  AC39 HEK-293T C393(1.13)  LDD1539  [2]
 LDCM0301  AC4 HEK-293T C452(1.06)  LDD1540  [2]
 LDCM0303  AC41 HEK-293T C599(1.06)  LDD1542  [2]
 LDCM0304  AC42 HEK-293T C452(1.20)  LDD1543  [2]
 LDCM0305  AC43 HEK-293T C317(1.01)  LDD1544  [2]
 LDCM0306  AC44 HEK-293T C452(1.35)  LDD1545  [2]
 LDCM0308  AC46 HEK-293T C452(1.09)  LDD1547  [2]
 LDCM0309  AC47 HEK-293T C393(1.11)  LDD1548  [2]
 LDCM0311  AC49 HEK-293T C599(1.04)  LDD1550  [2]
 LDCM0313  AC50 HEK-293T C452(1.08)  LDD1552  [2]
 LDCM0314  AC51 HEK-293T C317(1.00)  LDD1553  [2]
 LDCM0315  AC52 HEK-293T C452(1.06)  LDD1554  [2]
 LDCM0317  AC54 HEK-293T C452(1.04)  LDD1556  [2]
 LDCM0318  AC55 HEK-293T C393(1.17)  LDD1557  [2]
 LDCM0320  AC57 HEK-293T C599(1.02)  LDD1559  [2]
 LDCM0321  AC58 HEK-293T C452(1.03)  LDD1560  [2]
 LDCM0322  AC59 HEK-293T C317(1.11)  LDD1561  [2]
 LDCM0323  AC6 HEK-293T C452(0.98)  LDD1562  [2]
 LDCM0324  AC60 HEK-293T C452(1.14)  LDD1563  [2]
 LDCM0326  AC62 HEK-293T C452(1.09)  LDD1565  [2]
 LDCM0327  AC63 HEK-293T C393(1.26)  LDD1566  [2]
 LDCM0334  AC7 HEK-293T C393(1.03)  LDD1568  [2]
 LDCM0356  AKOS034007680 HEK-293T C599(1.06)  LDD1570  [2]
 LDCM0367  CL1 HEK-293T C452(1.11); C599(1.11); C496(1.05); C317(0.97)  LDD1571  [2]
 LDCM0368  CL10 HEK-293T C452(1.01)  LDD1572  [2]
 LDCM0369  CL100 HEK-293T C452(1.01)  LDD1573  [2]
 LDCM0370  CL101 HEK-293T C452(1.13); C599(1.01); C496(1.23); C317(0.98)  LDD1574  [2]
 LDCM0371  CL102 HEK-293T C452(1.01); C599(1.42)  LDD1575  [2]
 LDCM0372  CL103 HEK-293T C452(0.97)  LDD1576  [2]
 LDCM0373  CL104 HEK-293T C452(1.13)  LDD1577  [2]
 LDCM0374  CL105 HEK-293T C452(1.03); C599(1.06); C496(1.05); C317(0.90)  LDD1578  [2]
 LDCM0375  CL106 HEK-293T C452(1.06); C599(1.19)  LDD1579  [2]
 LDCM0376  CL107 HEK-293T C452(0.93)  LDD1580  [2]
 LDCM0377  CL108 HEK-293T C452(1.07)  LDD1581  [2]
 LDCM0378  CL109 HEK-293T C452(0.95); C599(1.09); C496(1.27); C317(1.04)  LDD1582  [2]
 LDCM0379  CL11 HEK-293T C393(0.98)  LDD1583  [2]
 LDCM0380  CL110 HEK-293T C452(0.95); C599(1.68)  LDD1584  [2]
 LDCM0381  CL111 HEK-293T C452(0.97)  LDD1585  [2]
 LDCM0382  CL112 HEK-293T C452(1.03)  LDD1586  [2]
 LDCM0383  CL113 HEK-293T C452(0.98); C599(0.95); C496(1.03); C317(0.88)  LDD1587  [2]
 LDCM0384  CL114 HEK-293T C452(0.96); C599(2.06)  LDD1588  [2]
 LDCM0385  CL115 HEK-293T C452(1.01)  LDD1589  [2]
 LDCM0386  CL116 HEK-293T C452(1.05)  LDD1590  [2]
 LDCM0387  CL117 HEK-293T C452(1.06); C599(0.91); C496(1.22); C317(1.06)  LDD1591  [2]
 LDCM0388  CL118 HEK-293T C452(1.05); C599(1.00)  LDD1592  [2]
 LDCM0389  CL119 HEK-293T C452(1.07)  LDD1593  [2]
 LDCM0391  CL120 HEK-293T C452(1.14)  LDD1595  [2]
 LDCM0392  CL121 HEK-293T C452(1.25); C599(0.88); C496(1.10); C317(0.95)  LDD1596  [2]
 LDCM0393  CL122 HEK-293T C452(1.00); C599(1.15)  LDD1597  [2]
 LDCM0394  CL123 HEK-293T C452(1.00)  LDD1598  [2]
 LDCM0395  CL124 HEK-293T C452(1.02)  LDD1599  [2]
 LDCM0396  CL125 HEK-293T C452(1.07); C599(0.87); C496(1.05); C317(0.95)  LDD1600  [2]
 LDCM0397  CL126 HEK-293T C452(1.02); C599(1.10)  LDD1601  [2]
 LDCM0398  CL127 HEK-293T C452(1.06)  LDD1602  [2]
 LDCM0399  CL128 HEK-293T C452(1.01)  LDD1603  [2]
 LDCM0400  CL13 HEK-293T C452(1.17); C599(0.98); C496(1.04); C317(0.94)  LDD1604  [2]
 LDCM0401  CL14 HEK-293T C452(0.95); C599(0.96)  LDD1605  [2]
 LDCM0402  CL15 HEK-293T C452(1.04)  LDD1606  [2]
 LDCM0403  CL16 HEK-293T C452(0.98)  LDD1607  [2]
 LDCM0404  CL17 HEK-293T C599(2.12)  LDD1608  [2]
 LDCM0405  CL18 HEK-293T C452(1.02)  LDD1609  [2]
 LDCM0406  CL19 HEK-293T C317(0.98)  LDD1610  [2]
 LDCM0407  CL2 HEK-293T C452(1.00); C599(0.96)  LDD1611  [2]
 LDCM0408  CL20 HEK-293T C452(1.15)  LDD1612  [2]
 LDCM0410  CL22 HEK-293T C452(1.04)  LDD1614  [2]
 LDCM0411  CL23 HEK-293T C393(1.14)  LDD1615  [2]
 LDCM0413  CL25 HEK-293T C452(0.87); C599(1.37); C496(1.10); C317(0.96)  LDD1617  [2]
 LDCM0414  CL26 HEK-293T C452(0.99); C599(0.99)  LDD1618  [2]
 LDCM0415  CL27 HEK-293T C452(1.01)  LDD1619  [2]
 LDCM0416  CL28 HEK-293T C452(1.02)  LDD1620  [2]
 LDCM0417  CL29 HEK-293T C599(1.04)  LDD1621  [2]
 LDCM0418  CL3 HEK-293T C452(0.95)  LDD1622  [2]
 LDCM0419  CL30 HEK-293T C452(1.07)  LDD1623  [2]
 LDCM0420  CL31 HEK-293T C317(1.12)  LDD1624  [2]
 LDCM0421  CL32 HEK-293T C452(1.00)  LDD1625  [2]
 LDCM0423  CL34 HEK-293T C452(1.02)  LDD1627  [2]
 LDCM0424  CL35 HEK-293T C393(0.95)  LDD1628  [2]
 LDCM0426  CL37 HEK-293T C452(0.91); C599(1.07); C496(0.95); C317(1.06)  LDD1630  [2]
 LDCM0428  CL39 HEK-293T C452(1.00)  LDD1632  [2]
 LDCM0429  CL4 HEK-293T C452(1.00)  LDD1633  [2]
 LDCM0430  CL40 HEK-293T C452(1.12)  LDD1634  [2]
 LDCM0431  CL41 HEK-293T C599(1.53)  LDD1635  [2]
 LDCM0432  CL42 HEK-293T C452(1.07)  LDD1636  [2]
 LDCM0433  CL43 HEK-293T C317(1.15)  LDD1637  [2]
 LDCM0434  CL44 HEK-293T C452(1.07)  LDD1638  [2]
 LDCM0436  CL46 HEK-293T C452(0.96)  LDD1640  [2]
 LDCM0437  CL47 HEK-293T C393(1.02)  LDD1641  [2]
 LDCM0439  CL49 HEK-293T C452(1.01); C599(1.04); C496(0.95); C317(1.08)  LDD1643  [2]
 LDCM0440  CL5 HEK-293T C599(1.07)  LDD1644  [2]
 LDCM0441  CL50 HEK-293T C452(1.00); C599(1.34)  LDD1645  [2]
 LDCM0443  CL52 HEK-293T C452(1.03)  LDD1646  [2]
 LDCM0444  CL53 HEK-293T C599(1.85)  LDD1647  [2]
 LDCM0445  CL54 HEK-293T C452(1.07)  LDD1648  [2]
 LDCM0446  CL55 HEK-293T C317(1.26)  LDD1649  [2]
 LDCM0447  CL56 HEK-293T C452(1.12)  LDD1650  [2]
 LDCM0449  CL58 HEK-293T C452(0.94)  LDD1652  [2]
 LDCM0450  CL59 HEK-293T C393(0.97)  LDD1653  [2]
 LDCM0451  CL6 HEK-293T C452(1.02)  LDD1654  [2]
 LDCM0453  CL61 HEK-293T C452(1.08); C599(1.03); C496(0.89); C317(1.05)  LDD1656  [2]
 LDCM0454  CL62 HEK-293T C452(0.95); C599(0.96)  LDD1657  [2]
 LDCM0455  CL63 HEK-293T C452(0.92)  LDD1658  [2]
 LDCM0456  CL64 HEK-293T C452(1.12)  LDD1659  [2]
 LDCM0457  CL65 HEK-293T C599(1.06)  LDD1660  [2]
 LDCM0458  CL66 HEK-293T C452(1.23)  LDD1661  [2]
 LDCM0459  CL67 HEK-293T C317(1.15)  LDD1662  [2]
 LDCM0460  CL68 HEK-293T C452(1.09)  LDD1663  [2]
 LDCM0462  CL7 HEK-293T C317(1.20)  LDD1665  [2]
 LDCM0463  CL70 HEK-293T C452(1.06)  LDD1666  [2]
 LDCM0464  CL71 HEK-293T C393(0.90)  LDD1667  [2]
 LDCM0466  CL73 HEK-293T C452(0.97); C599(1.17); C496(1.12); C317(1.05)  LDD1669  [2]
 LDCM0467  CL74 HEK-293T C452(0.98); C599(0.94)  LDD1670  [2]
 LDCM0469  CL76 HEK-293T C452(1.09)  LDD1672  [2]
 LDCM0470  CL77 HEK-293T C599(1.78)  LDD1673  [2]
 LDCM0471  CL78 HEK-293T C452(1.15)  LDD1674  [2]
 LDCM0472  CL79 HEK-293T C317(1.11)  LDD1675  [2]
 LDCM0473  CL8 HEK-293T C452(0.96)  LDD1676  [2]
 LDCM0474  CL80 HEK-293T C452(1.06)  LDD1677  [2]
 LDCM0476  CL82 HEK-293T C452(1.10)  LDD1679  [2]
 LDCM0477  CL83 HEK-293T C393(0.95)  LDD1680  [2]
 LDCM0479  CL85 HEK-293T C452(1.17); C599(1.02); C496(0.77); C317(0.96)  LDD1682  [2]
 LDCM0480  CL86 HEK-293T C452(0.92); C599(1.04)  LDD1683  [2]
 LDCM0481  CL87 HEK-293T C452(1.16)  LDD1684  [2]
 LDCM0482  CL88 HEK-293T C452(1.17)  LDD1685  [2]
 LDCM0483  CL89 HEK-293T C599(1.13)  LDD1686  [2]
 LDCM0485  CL90 HEK-293T C452(1.02)  LDD1688  [2]
 LDCM0486  CL91 HEK-293T C317(1.08)  LDD1689  [2]
 LDCM0487  CL92 HEK-293T C452(1.12)  LDD1690  [2]
 LDCM0489  CL94 HEK-293T C452(1.02)  LDD1692  [2]
 LDCM0490  CL95 HEK-293T C393(0.99)  LDD1693  [2]
 LDCM0492  CL97 HEK-293T C452(1.00); C599(1.24); C496(1.06); C317(1.11)  LDD1695  [2]
 LDCM0493  CL98 HEK-293T C452(1.06); C599(1.19)  LDD1696  [2]
 LDCM0494  CL99 HEK-293T C452(1.03)  LDD1697  [2]
 LDCM0495  E2913 HEK-293T C452(1.02)  LDD1698  [2]
 LDCM0468  Fragment33 HEK-293T C452(0.98)  LDD1671  [2]
 LDCM0427  Fragment51 HEK-293T C452(0.95); C599(1.05)  LDD1631  [2]
 LDCM0022  KB02 HEK-293T C452(0.90); C599(0.63)  LDD1492  [2]
 LDCM0023  KB03 HEK-293T C452(1.08); C599(0.79)  LDD1497  [2]
 LDCM0024  KB05 OCI-AML-2 C317(1.91)  LDD3370  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Hepatocyte growth factor receptor (MET) Tyr protein kinase family P08581
Mast/stem cell growth factor receptor Kit (KIT) Tyr protein kinase family P10721
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Breast cancer anti-estrogen resistance protein 1 (BCAR1) CAS family P56945

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402