Details of the Target

General Information of Target

Target ID LDTP08730
Target Name N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase (GCNT2)
Gene Name GCNT2
Gene ID 2651
Synonyms
GCNT5; II; NACGT1; N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase; N-acetylglucosaminyltransferase; EC 2.4.1.150; I-branching enzyme; IGNT
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MMGSWKHCLFSASLISALIFVFVYNTELWENKRFLRAALSNASLLAEACHQIFEGKVFYP
TENALKTTLDEATCYEYMVRSHYVTETLSEEEAGFPLAYTVTIHKDFGTFERLFRAIYMP
QNVYCVHLDQKATDAFKGAVKQLLSCFPNAFLASKKESVVYGGISRLQADLNCLEDLVAS
EVPWKYVINTCGQDFPLKTNREIVQYLKGFKGKNITPGVLPPDHAVGRTKYVHQELLNHK
NSYVIKTTKLKTPPPHDMVIYFGTAYVALTRDFANFVLQDQLALDLLSWSKDTYSPDEHF
WVTLNRIPGVPGSMPNASWTGNLRAIKWSDMEDRHGGCHGHYVHGICIYGNGDLKWLVNS
PSLFANKFELNTYPLTVECLELRHRERTLNQSETAIQPSWYF
Target Bioclass
Enzyme
Family
Glycosyltransferase 14 family
Subcellular location
Golgi apparatus membrane
Function
Branching enzyme that converts linear into branched poly-N-acetyllactosaminoglycans. Introduces the blood group I antigen during embryonic development. It is closely associated with the development and maturation of erythroid cells.; [Isoform C]: Determines the expression of the blood group I antigen in erythrocytes.
Uniprot ID
Q8N0V5
Ensemble ID
ENST00000265012.5
HGNC ID
HGNC:4204

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 Deletion: p.T376LfsTer5 .
JM1 SNV: p.Y118D .
KMS11 Substitution: p.P253T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C191(1.07)  LDD0078  [1]
CY-1
 Probe Info  		E369(0.00); E378(0.00); E381(0.00)  LDD0246  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0216  AC100 PaTu 8988t C191(0.84)  LDD1095  [1]
 LDCM0217  AC101 PaTu 8988t C191(0.90)  LDD1096  [1]
 LDCM0218  AC102 PaTu 8988t C191(1.29)  LDD1097  [1]
 LDCM0219  AC103 PaTu 8988t C191(1.25)  LDD1098  [1]
 LDCM0220  AC104 PaTu 8988t C191(0.86)  LDD1099  [1]
 LDCM0221  AC105 PaTu 8988t C191(1.18)  LDD1100  [1]
 LDCM0222  AC106 PaTu 8988t C191(1.36)  LDD1101  [1]
 LDCM0223  AC107 PaTu 8988t C191(0.93)  LDD1102  [1]
 LDCM0224  AC108 PaTu 8988t C191(1.17)  LDD1103  [1]
 LDCM0225  AC109 PaTu 8988t C191(1.14)  LDD1104  [1]
 LDCM0227  AC110 PaTu 8988t C191(1.03)  LDD1106  [1]
 LDCM0228  AC111 PaTu 8988t C191(1.37)  LDD1107  [1]
 LDCM0229  AC112 PaTu 8988t C191(1.24)  LDD1108  [1]
 LDCM0230  AC113 PaTu 8988t C191(0.91)  LDD1109  [1]
 LDCM0231  AC114 PaTu 8988t C191(1.19)  LDD1110  [1]
 LDCM0232  AC115 PaTu 8988t C191(0.93)  LDD1111  [1]
 LDCM0233  AC116 PaTu 8988t C191(1.29)  LDD1112  [1]
 LDCM0234  AC117 PaTu 8988t C191(0.97)  LDD1113  [1]
 LDCM0235  AC118 PaTu 8988t C191(1.01)  LDD1114  [1]
 LDCM0236  AC119 PaTu 8988t C191(1.20)  LDD1115  [1]
 LDCM0238  AC120 PaTu 8988t C191(1.09)  LDD1117  [1]
 LDCM0239  AC121 PaTu 8988t C191(1.33)  LDD1118  [1]
 LDCM0240  AC122 PaTu 8988t C191(0.90)  LDD1119  [1]
 LDCM0241  AC123 PaTu 8988t C191(1.06)  LDD1120  [1]
 LDCM0242  AC124 PaTu 8988t C191(1.38)  LDD1121  [1]
 LDCM0243  AC125 PaTu 8988t C191(0.90)  LDD1122  [1]
 LDCM0244  AC126 PaTu 8988t C191(0.88)  LDD1123  [1]
 LDCM0245  AC127 PaTu 8988t C191(1.24)  LDD1124  [1]
 LDCM0246  AC128 PaTu 8988t C191(1.33)  LDD1125  [1]
 LDCM0247  AC129 PaTu 8988t C191(1.09)  LDD1126  [1]
 LDCM0249  AC130 PaTu 8988t C191(0.98)  LDD1128  [1]
 LDCM0250  AC131 PaTu 8988t C191(1.18)  LDD1129  [1]
 LDCM0251  AC132 PaTu 8988t C191(1.11)  LDD1130  [1]
 LDCM0252  AC133 PaTu 8988t C191(2.04)  LDD1131  [1]
 LDCM0253  AC134 PaTu 8988t C191(1.44)  LDD1132  [1]
 LDCM0254  AC135 PaTu 8988t C191(1.70)  LDD1133  [1]
 LDCM0255  AC136 PaTu 8988t C191(1.29)  LDD1134  [1]
 LDCM0256  AC137 PaTu 8988t C191(1.51)  LDD1135  [1]
 LDCM0257  AC138 PaTu 8988t C191(1.17)  LDD1136  [1]
 LDCM0258  AC139 PaTu 8988t C191(1.46)  LDD1137  [1]
 LDCM0260  AC140 PaTu 8988t C191(1.13)  LDD1139  [1]
 LDCM0261  AC141 PaTu 8988t C191(0.99)  LDD1140  [1]
 LDCM0262  AC142 PaTu 8988t C191(1.18)  LDD1141  [1]
 LDCM0263  AC143 PaTu 8988t C191(1.03)  LDD1142  [1]
 LDCM0264  AC144 PaTu 8988t C191(0.88)  LDD1143  [1]
 LDCM0265  AC145 PaTu 8988t C191(0.97)  LDD1144  [1]
 LDCM0266  AC146 PaTu 8988t C191(0.89)  LDD1145  [1]
 LDCM0267  AC147 PaTu 8988t C191(0.84)  LDD1146  [1]
 LDCM0268  AC148 PaTu 8988t C191(1.34)  LDD1147  [1]
 LDCM0269  AC149 PaTu 8988t C191(1.44)  LDD1148  [1]
 LDCM0271  AC150 PaTu 8988t C191(0.94)  LDD1150  [1]
 LDCM0272  AC151 PaTu 8988t C191(1.08)  LDD1151  [1]
 LDCM0273  AC152 PaTu 8988t C191(1.04)  LDD1152  [1]
 LDCM0274  AC153 PaTu 8988t C191(1.37)  LDD1153  [1]
 LDCM0621  AC154 PaTu 8988t C191(1.07)  LDD2166  [1]
 LDCM0622  AC155 PaTu 8988t C191(0.99)  LDD2167  [1]
 LDCM0623  AC156 PaTu 8988t C191(1.04)  LDD2168  [1]
 LDCM0624  AC157 PaTu 8988t C191(0.82)  LDD2169  [1]
 LDCM0308  AC46 PaTu 8988t C191(1.45)  LDD1187  [1]
 LDCM0309  AC47 PaTu 8988t C191(1.20)  LDD1188  [1]
 LDCM0310  AC48 PaTu 8988t C191(1.85)  LDD1189  [1]
 LDCM0311  AC49 PaTu 8988t C191(1.29)  LDD1190  [1]
 LDCM0313  AC50 PaTu 8988t C191(1.30)  LDD1192  [1]
 LDCM0314  AC51 PaTu 8988t C191(1.30)  LDD1193  [1]
 LDCM0315  AC52 PaTu 8988t C191(1.41)  LDD1194  [1]
 LDCM0316  AC53 PaTu 8988t C191(1.56)  LDD1195  [1]
 LDCM0317  AC54 PaTu 8988t C191(1.58)  LDD1196  [1]
 LDCM0318  AC55 PaTu 8988t C191(1.22)  LDD1197  [1]
 LDCM0319  AC56 PaTu 8988t C191(1.41)  LDD1198  [1]
 LDCM0349  AC83 PaTu 8988t C191(1.05)  LDD1228  [1]
 LDCM0350  AC84 PaTu 8988t C191(0.96)  LDD1229  [1]
 LDCM0351  AC85 PaTu 8988t C191(0.94)  LDD1230  [1]
 LDCM0352  AC86 PaTu 8988t C191(1.18)  LDD1231  [1]
 LDCM0353  AC87 PaTu 8988t C191(0.92)  LDD1232  [1]
 LDCM0354  AC88 PaTu 8988t C191(0.87)  LDD1233  [1]
 LDCM0355  AC89 PaTu 8988t C191(0.94)  LDD1234  [1]
 LDCM0357  AC90 PaTu 8988t C191(1.14)  LDD1236  [1]
 LDCM0358  AC91 PaTu 8988t C191(0.98)  LDD1237  [1]
 LDCM0359  AC92 PaTu 8988t C191(0.96)  LDD1238  [1]
 LDCM0360  AC93 PaTu 8988t C191(0.89)  LDD1239  [1]
 LDCM0361  AC94 PaTu 8988t C191(0.94)  LDD1240  [1]
 LDCM0362  AC95 PaTu 8988t C191(1.01)  LDD1241  [1]
 LDCM0363  AC96 PaTu 8988t C191(0.91)  LDD1242  [1]
 LDCM0364  AC97 PaTu 8988t C191(1.07)  LDD1243  [1]
 LDCM0365  AC98 PaTu 8988t C191(1.04)  LDD1244  [1]
 LDCM0366  AC99 PaTu 8988t C191(1.12)  LDD1245  [1]
 LDCM0020  ARS-1620 HCC44 C191(1.07)  LDD0078  [1]
 LDCM0392  CL121 PaTu 8988t C191(1.17)  LDD1271  [1]
 LDCM0393  CL122 PaTu 8988t C191(2.03)  LDD1272  [1]
 LDCM0394  CL123 PaTu 8988t C191(1.43)  LDD1273  [1]
 LDCM0395  CL124 PaTu 8988t C191(1.60)  LDD1274  [1]
 LDCM0403  CL16 PaTu 8988t C191(1.39)  LDD1282  [1]
 LDCM0404  CL17 PaTu 8988t C191(1.25)  LDD1283  [1]
 LDCM0405  CL18 PaTu 8988t C191(1.29)  LDD1284  [1]
 LDCM0406  CL19 PaTu 8988t C191(1.26)  LDD1285  [1]
 LDCM0408  CL20 PaTu 8988t C191(1.33)  LDD1287  [1]
 LDCM0409  CL21 PaTu 8988t C191(1.18)  LDD1288  [1]
 LDCM0410  CL22 PaTu 8988t C191(1.31)  LDD1289  [1]
 LDCM0411  CL23 PaTu 8988t C191(1.37)  LDD1290  [1]
 LDCM0412  CL24 PaTu 8988t C191(1.25)  LDD1291  [1]
 LDCM0413  CL25 PaTu 8988t C191(1.43)  LDD1292  [1]
 LDCM0414  CL26 PaTu 8988t C191(1.13)  LDD1293  [1]
 LDCM0415  CL27 PaTu 8988t C191(1.30)  LDD1294  [1]
 LDCM0416  CL28 PaTu 8988t C191(1.69)  LDD1295  [1]
 LDCM0417  CL29 PaTu 8988t C191(1.23)  LDD1296  [1]
 LDCM0419  CL30 PaTu 8988t C191(1.32)  LDD1298  [1]
 LDCM0420  CL31 PaTu 8988t C191(1.28)  LDD1299  [1]
 LDCM0421  CL32 PaTu 8988t C191(1.75)  LDD1300  [1]
 LDCM0422  CL33 PaTu 8988t C191(1.66)  LDD1301  [1]
 LDCM0423  CL34 PaTu 8988t C191(1.56)  LDD1302  [1]
 LDCM0424  CL35 PaTu 8988t C191(1.76)  LDD1303  [1]
 LDCM0425  CL36 PaTu 8988t C191(1.81)  LDD1304  [1]
 LDCM0426  CL37 PaTu 8988t C191(2.45)  LDD1305  [1]
 LDCM0428  CL39 PaTu 8988t C191(1.54)  LDD1307  [1]
 LDCM0430  CL40 PaTu 8988t C191(1.33)  LDD1309  [1]
 LDCM0431  CL41 PaTu 8988t C191(2.09)  LDD1310  [1]
 LDCM0432  CL42 PaTu 8988t C191(2.43)  LDD1311  [1]
 LDCM0433  CL43 PaTu 8988t C191(3.65)  LDD1312  [1]
 LDCM0434  CL44 PaTu 8988t C191(1.96)  LDD1313  [1]
 LDCM0435  CL45 PaTu 8988t C191(1.47)  LDD1314  [1]
 LDCM0453  CL61 PaTu 8988t C191(1.07)  LDD1332  [1]
 LDCM0454  CL62 PaTu 8988t C191(0.83)  LDD1333  [1]
 LDCM0455  CL63 PaTu 8988t C191(0.83)  LDD1334  [1]
 LDCM0456  CL64 PaTu 8988t C191(0.98)  LDD1335  [1]
 LDCM0457  CL65 PaTu 8988t C191(1.01)  LDD1336  [1]
 LDCM0458  CL66 PaTu 8988t C191(1.04)  LDD1337  [1]
 LDCM0459  CL67 PaTu 8988t C191(1.31)  LDD1338  [1]
 LDCM0460  CL68 PaTu 8988t C191(1.46)  LDD1339  [1]
 LDCM0461  CL69 PaTu 8988t C191(1.24)  LDD1340  [1]
 LDCM0463  CL70 PaTu 8988t C191(1.21)  LDD1342  [1]
 LDCM0464  CL71 PaTu 8988t C191(0.83)  LDD1343  [1]
 LDCM0465  CL72 PaTu 8988t C191(0.73)  LDD1344  [1]
 LDCM0466  CL73 PaTu 8988t C191(1.29)  LDD1345  [1]
 LDCM0467  CL74 PaTu 8988t C191(0.88)  LDD1346  [1]
 LDCM0468  Fragment33 PaTu 8988t C191(0.86)  LDD1347  [1]
 LDCM0427  Fragment51 PaTu 8988t C191(2.11)  LDD1306  [1]
 LDCM0022  KB02 A2058 C191(4.59)  LDD2253  [3]
 LDCM0023  KB03 A2058 C191(2.51)  LDD2670  [3]
 LDCM0024  KB05 SKMEL3 C191(3.56)  LDD3324  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Fibronectin type III domain-containing protein 9 (FNDC9) . Q8TBE3

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840