Details of the Target

General Information of Target

Target ID LDTP08643
Target Name Fanconi anemia group M protein (FANCM)
Gene Name FANCM
Gene ID 57697
Synonyms
KIAA1596; Fanconi anemia group M protein; Protein FACM; EC 3.6.4.13; ATP-dependent RNA helicase FANCM; Fanconi anemia-associated polypeptide of 250 kDa; FAAP250; Protein Hef ortholog
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MSGRQRTLFQTWGSSISRSSGTPGCSSGTERPQSPGSSKAPLPAAAEAQLESDDDVLLVA
AYEAERQLCLENGGFCTSAGALWIYPTNCPVRDYQLHISRAALFCNTLVCLPTGLGKTFI
AAVVMYNFYRWFPSGKVVFMAPTKPLVTQQIEACYQVMGIPQSHMAEMTGSTQASTRKEI
WCSKRVLFLTPQVMVNDLSRGACPAAEIKCLVIDEAHKALGNYAYCQVVRELVKYTNHFR
ILALSATPGSDIKAVQQVITNLLIGQIELRSEDSPDILTYSHERKVEKLIVPLGEELAAI
QKTYIQILESFARSLIQRNVLMRRDIPNLTKYQIILARDQFRKNPSPNIVGIQQGIIEGE
FAICISLYHGYELLQQMGMRSLYFFLCGIMDGTKGMTRSKNELGRNEDFMKLYNHLECMF
ARTRSTSANGISAIQQGDKNKKFVYSHPKLKKLEEVVIEHFKSWNAENTTEKKRDETRVM
IFSSFRDSVQEIAEMLSQHQPIIRVMTFVGHASGKSTKGFTQKEQLEVVKQFRDGGYNTL
VSTCVGEEGLDIGEVDLIICFDSQKSPIRLVQRMGRTGRKRQGRIVIILSEGREERIYNQ
SQSNKRSIYKAISSNRQVLHFYQRSPRMVPDGINPKLHKMFITHGVYEPEKPSRNLQRKS
SIFSYRDGMRQSSLKKDWFLSEEEFKLWNRLYRLRDSDEIKEITLPQVQFSSLQNEENKP
AQESTTGIHQLSLSEWRLWQDHPLPTHQVDHSDRCRHFIGLMQMIEGMRHEEGECSYELE
VESYLQMEDVTSTFIAPRNESNNLASDTFITHKKSSFIKNINQGSSSSVIESDEECAEIV
KQTHIKPTKIVSLKKKVSKEIKKDQLKKENNHGIIDSVDNDRNSTVENIFQEDLPNDKRT
SDTDEIAATCTINENVIKEPCVLLTECQFTNKSTSSLAGNVLDSGYNSFNDEKSVSSNLF
LPFEEELYIVRTDDQFYNCHSLTKEVLANVERFLSYSPPPLSGLSDLEYEIAKGTALENL
LFLPCAEHLRSDKCTCLLSHSAVNSQQNLELNSLKCINYPSEKSCLYDIPNDNISDEPSL
CDCDVHKHNQNENLVPNNRVQIHRSPAQNLVGENNHDVDNSDLPVLSTDQDESLLLFEDV
NTEFDDVSLSPLNSKSESLPVSDKTAISETPLVSQFLISDELLLDNNSELQDQITRDANS
FKSRDQRGVQEEKVKNHEDIFDCSRDLFSVTFDLGFCSPDSDDEILEHTSDSNRPLDDLY
GRYLEIKEISDANYVSNQALIPRDHSKNFTSGTVIIPSNEDMQNPNYVHLPLSAAKNEEL
LSPGYSQFSLPVQKKVMSTPLSKSNTLNSFSKIRKEILKTPDSSKEKVNLQRFKEALNST
FDYSEFSLEKSKSSGPMYLHKSCHSVEDGQLLTSNESEDDEIFRRKVKRAKGNVLNSPED
QKNSEVDSPLHAVKKRRFPINRSELSSSDESENFPKPCSQLEDFKVCNGNARRGIKVPKR
QSHLKHVARKFLDDEAELSEEDAEYVSSDENDESENEQDSSLLDFLNDETQLSQAINDSE
MRAIYMKSLRSPMMNNKYKMIHKTHKNINIFSQIPEQDETYLEDSFCVDEEESCKGQSSE
EEVCVDFNLITDDCFANSKKYKTRRAVMLKEMMEQNCAHSKKKLSRIILPDDSSEEENNV
NDKRESNIAVNPSTVKKNKQQDHCLNSVPSGSSAQSKVRSTPRVNPLAKQSKQTSLNLKD
TISEVSDFKPQNHNEVQSTTPPFTTVDSQKDCRKFPVPQKDGSALEDSSTSGASCSKSRP
HLAGTHTSLRLPQEGKGTCILVGGHEITSGLEVISSLRAIHGLQVEVCPLNGCDYIVSNR
MVVERRSQSEMLNSVNKNKFIEQIQHLQSMFERICVIVEKDREKTGDTSRMFRRTKSYDS
LLTTLIGAGIRILFSSCQEETADLLKELSLVEQRKNVGIHVPTVVNSNKSEALQFYLSIP
NISYITALNMCHQFSSVKRMANSSLQEISMYAQVTHQKAEEIYRYIHYVFDIQMLPNDLN
QDRLKSDI
Target Bioclass
Enzyme
Family
DEAD box helicase family, DEAH subfamily, FANCM sub-subfamily
Subcellular location
Nucleus
Function
DNA-dependent ATPase component of the Fanconi anemia (FA) core complex. Required for the normal activation of the FA pathway, leading to monoubiquitination of the FANCI-FANCD2 complex in response to DNA damage, cellular resistance to DNA cross-linking drugs, and prevention of chromosomal breakage. In complex with CENPS and CENPX, binds double-stranded DNA (dsDNA), fork-structured DNA (fsDNA) and Holliday junction substrates. Its ATP-dependent DNA branch migration activity can process branched DNA structures such as a movable replication fork. This activity is strongly stimulated in the presence of CENPS and CENPX. In complex with FAAP24, efficiently binds to single-strand DNA (ssDNA), splayed-arm DNA, and 3'-flap substrates. In vitro, on its own, strongly binds ssDNA oligomers and weakly fsDNA, but does not bind to dsDNA.
Uniprot ID
Q8IYD8
Ensemble ID
ENST00000267430.10
HGNC ID
HGNC:23168

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
769P Deletion: p.K1355RfsTer14 .
ABC1 SNV: p.G1958C .
CCLP1 SNV: p.P1096L .
FADU SNV: p.S1618Ter .
HCT15 SNV: p.C110R; p.Q1757H .
HEC1 SNV: p.L82M .
HEC1B SNV: p.L82M .
HSC3 Deletion: p.I1930LfsTer2 .
HT115 SNV: p.E554Ter .
Ishikawa (Heraklio) 02 ER Insertion: p.E735LfsTer26 .
JURKAT SNV: p.A1107T .
KNS81FD Deletion: p.T1293LfsTer20 .
LN229 SNV: p.L367S .
LS123 SNV: p.T577I .
LS180 SNV: p.M1566T .
MEWO SNV: p.A224V; p.Y1059N .
MOLT4 SNV: p.Y304H .
NCIH661 SNV: p.S1665F .
NCIH716 SNV: p.T577I .
OCILY3 SNV: p.N1114K .
PC14 SNV: p.L1785V .
PC9 SNV: p.L1785V .
SNU1 Deletion: p.M1990VfsTer12
SNV: p.V1125A		 .
SW1783 SNV: p.R533H .
TF1 SNV: p.P1255R .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C921(1.01); C927(1.01)  LDD0078  [1]
IPM
 Probe Info  		C25(0.00); C1025(0.00)  LDD2156  [2]
YN-1
 Probe Info  		D1907(0.00); D1205(0.00)  LDD0447  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C927(1.48)  LDD0812  [1]
 LDCM0276  AC17 HEK-293T C921(0.99); C927(1.14)  LDD0874  [1]
 LDCM0277  AC18 HEK-293T C921(1.05); C927(0.96)  LDD0875  [1]
 LDCM0278  AC19 HEK-293T C921(0.88); C927(1.56)  LDD0876  [1]
 LDCM0279  AC2 HEK-293T C927(1.88)  LDD0877  [1]
 LDCM0280  AC20 HEK-293T C921(0.98); C927(1.16)  LDD0878  [1]
 LDCM0281  AC21 HEK-293T C921(1.02); C927(0.84)  LDD0879  [1]
 LDCM0282  AC22 HEK-293T C921(1.04); C927(0.75)  LDD0880  [1]
 LDCM0283  AC23 HEK-293T C921(1.21); C927(1.12)  LDD0881  [1]
 LDCM0284  AC24 HEK-293T C921(0.98); C927(1.16)  LDD0882  [1]
 LDCM0290  AC3 HEK-293T C927(1.24)  LDD0888  [1]
 LDCM0301  AC4 HEK-293T C927(1.35)  LDD0899  [1]
 LDCM0308  AC46 HEK-293T C921(0.92); C927(0.86)  LDD0906  [1]
 LDCM0309  AC47 HEK-293T C921(0.97); C927(0.99)  LDD0907  [1]
 LDCM0310  AC48 HEK-293T C921(1.03); C927(0.88)  LDD0908  [1]
 LDCM0311  AC49 HEK-293T C921(1.00); C927(1.04)  LDD0909  [1]
 LDCM0312  AC5 HEK-293T C927(1.62)  LDD0910  [1]
 LDCM0313  AC50 HEK-293T C921(1.04); C927(1.09)  LDD0911  [1]
 LDCM0314  AC51 HEK-293T C921(1.08); C927(1.01)  LDD0912  [1]
 LDCM0315  AC52 HEK-293T C921(1.02); C927(1.00)  LDD0913  [1]
 LDCM0316  AC53 HEK-293T C921(1.00); C927(1.00)  LDD0914  [1]
 LDCM0317  AC54 HEK-293T C921(1.00); C927(0.86)  LDD0915  [1]
 LDCM0318  AC55 HEK-293T C921(1.11); C927(1.10)  LDD0916  [1]
 LDCM0319  AC56 HEK-293T C921(1.08); C927(1.00)  LDD0917  [1]
 LDCM0320  AC57 HEK-293T C921(0.95); C927(0.95)  LDD0918  [1]
 LDCM0321  AC58 HEK-293T C921(1.01); C927(1.01)  LDD0919  [1]
 LDCM0322  AC59 HEK-293T C921(1.00); C927(1.07)  LDD0920  [1]
 LDCM0324  AC60 HEK-293T C921(0.94); C927(0.87)  LDD0922  [1]
 LDCM0325  AC61 HEK-293T C921(1.13); C927(1.05)  LDD0923  [1]
 LDCM0326  AC62 HEK-293T C921(1.27); C927(1.10)  LDD0924  [1]
 LDCM0327  AC63 HEK-293T C921(1.18); C927(1.18)  LDD0925  [1]
 LDCM0328  AC64 HEK-293T C921(0.99); C927(1.17)  LDD0926  [1]
 LDCM0329  AC65 HEK-293T C921(1.21); C927(1.28)  LDD0927  [1]
 LDCM0330  AC66 HEK-293T C921(1.12); C927(1.17)  LDD0928  [1]
 LDCM0331  AC67 HEK-293T C921(1.15); C927(1.20)  LDD0929  [1]
 LDCM0332  AC68 HEK-293T C927(0.97)  LDD0930  [1]
 LDCM0333  AC69 HEK-293T C927(1.11)  LDD0931  [1]
 LDCM0335  AC70 HEK-293T C927(1.13)  LDD0933  [1]
 LDCM0336  AC71 HEK-293T C927(1.08)  LDD0934  [1]
 LDCM0337  AC72 HEK-293T C927(0.96)  LDD0935  [1]
 LDCM0338  AC73 HEK-293T C927(0.92)  LDD0936  [1]
 LDCM0339  AC74 HEK-293T C927(0.97)  LDD0937  [1]
 LDCM0340  AC75 HEK-293T C927(1.02)  LDD0938  [1]
 LDCM0341  AC76 HEK-293T C927(1.02)  LDD0939  [1]
 LDCM0342  AC77 HEK-293T C927(1.30)  LDD0940  [1]
 LDCM0343  AC78 HEK-293T C927(1.04)  LDD0941  [1]
 LDCM0344  AC79 HEK-293T C927(0.96)  LDD0942  [1]
 LDCM0346  AC80 HEK-293T C927(1.07)  LDD0944  [1]
 LDCM0347  AC81 HEK-293T C927(0.99)  LDD0945  [1]
 LDCM0348  AC82 HEK-293T C927(1.28)  LDD0946  [1]
 LDCM0349  AC83 HEK-293T C927(0.64)  LDD0947  [1]
 LDCM0350  AC84 HEK-293T C927(1.19)  LDD0948  [1]
 LDCM0351  AC85 HEK-293T C927(0.71)  LDD0949  [1]
 LDCM0352  AC86 HEK-293T C927(0.91)  LDD0950  [1]
 LDCM0353  AC87 HEK-293T C927(1.48)  LDD0951  [1]
 LDCM0354  AC88 HEK-293T C927(0.92)  LDD0952  [1]
 LDCM0355  AC89 HEK-293T C927(1.28)  LDD0953  [1]
 LDCM0357  AC90 HEK-293T C927(1.58)  LDD0955  [1]
 LDCM0358  AC91 HEK-293T C927(0.85)  LDD0956  [1]
 LDCM0359  AC92 HEK-293T C927(0.75)  LDD0957  [1]
 LDCM0360  AC93 HEK-293T C927(1.02)  LDD0958  [1]
 LDCM0361  AC94 HEK-293T C927(2.44)  LDD0959  [1]
 LDCM0362  AC95 HEK-293T C927(0.97)  LDD0960  [1]
 LDCM0363  AC96 HEK-293T C927(1.17)  LDD0961  [1]
 LDCM0364  AC97 HEK-293T C927(1.78)  LDD0962  [1]
 LDCM0020  ARS-1620 HCC44 C921(1.01); C927(1.01)  LDD0078  [1]
 LDCM0369  CL100 HEK-293T C927(1.41)  LDD0967  [1]
 LDCM0374  CL105 HEK-293T C921(0.86); C927(0.91)  LDD0972  [1]
 LDCM0375  CL106 HEK-293T C921(1.02); C927(0.91)  LDD0973  [1]
 LDCM0376  CL107 HEK-293T C921(0.83); C927(0.93)  LDD0974  [1]
 LDCM0377  CL108 HEK-293T C921(0.92); C927(0.88)  LDD0975  [1]
 LDCM0378  CL109 HEK-293T C921(1.06); C927(1.51)  LDD0976  [1]
 LDCM0380  CL110 HEK-293T C921(1.09); C927(1.26)  LDD0978  [1]
 LDCM0381  CL111 HEK-293T C921(1.14); C927(0.90)  LDD0979  [1]
 LDCM0392  CL121 HEK-293T C921(1.02); C927(0.92)  LDD0990  [1]
 LDCM0393  CL122 HEK-293T C921(1.08); C927(0.97)  LDD0991  [1]
 LDCM0394  CL123 HEK-293T C921(1.33); C927(1.43)  LDD0992  [1]
 LDCM0395  CL124 HEK-293T C921(0.93); C927(0.87)  LDD0993  [1]
 LDCM0396  CL125 HEK-293T C921(1.04); C927(0.95)  LDD0994  [1]
 LDCM0397  CL126 HEK-293T C921(0.97); C927(0.89)  LDD0995  [1]
 LDCM0398  CL127 HEK-293T C921(0.94); C927(0.88)  LDD0996  [1]
 LDCM0399  CL128 HEK-293T C921(1.04); C927(1.02)  LDD0997  [1]
 LDCM0403  CL16 HEK-293T C927(1.27)  LDD1001  [1]
 LDCM0404  CL17 HEK-293T C927(0.86)  LDD1002  [1]
 LDCM0405  CL18 HEK-293T C927(0.86)  LDD1003  [1]
 LDCM0406  CL19 HEK-293T C927(0.70)  LDD1004  [1]
 LDCM0408  CL20 HEK-293T C927(1.10)  LDD1006  [1]
 LDCM0409  CL21 HEK-293T C927(0.95)  LDD1007  [1]
 LDCM0410  CL22 HEK-293T C927(0.77)  LDD1008  [1]
 LDCM0411  CL23 HEK-293T C927(0.97)  LDD1009  [1]
 LDCM0412  CL24 HEK-293T C927(0.87)  LDD1010  [1]
 LDCM0413  CL25 HEK-293T C927(0.83)  LDD1011  [1]
 LDCM0414  CL26 HEK-293T C927(1.12)  LDD1012  [1]
 LDCM0415  CL27 HEK-293T C927(1.02)  LDD1013  [1]
 LDCM0416  CL28 HEK-293T C927(0.88)  LDD1014  [1]
 LDCM0417  CL29 HEK-293T C927(0.94)  LDD1015  [1]
 LDCM0419  CL30 HEK-293T C927(0.85)  LDD1017  [1]
 LDCM0420  CL31 HEK-293T C927(1.04)  LDD1018  [1]
 LDCM0421  CL32 HEK-293T C927(1.44)  LDD1019  [1]
 LDCM0422  CL33 HEK-293T C927(0.98)  LDD1020  [1]
 LDCM0423  CL34 HEK-293T C927(1.07)  LDD1021  [1]
 LDCM0424  CL35 HEK-293T C927(1.26)  LDD1022  [1]
 LDCM0425  CL36 HEK-293T C927(1.20)  LDD1023  [1]
 LDCM0426  CL37 HEK-293T C927(1.15)  LDD1024  [1]
 LDCM0428  CL39 HEK-293T C927(1.23)  LDD1026  [1]
 LDCM0430  CL40 HEK-293T C927(1.31)  LDD1028  [1]
 LDCM0431  CL41 HEK-293T C927(1.12)  LDD1029  [1]
 LDCM0432  CL42 HEK-293T C927(1.11)  LDD1030  [1]
 LDCM0433  CL43 HEK-293T C927(1.20)  LDD1031  [1]
 LDCM0434  CL44 HEK-293T C927(1.31)  LDD1032  [1]
 LDCM0435  CL45 HEK-293T C927(1.32)  LDD1033  [1]
 LDCM0436  CL46 HEK-293T C921(1.06); C927(1.13)  LDD1034  [1]
 LDCM0437  CL47 HEK-293T C921(0.90); C927(0.98)  LDD1035  [1]
 LDCM0438  CL48 HEK-293T C921(0.91); C927(1.05)  LDD1036  [1]
 LDCM0439  CL49 HEK-293T C921(1.21); C927(1.33)  LDD1037  [1]
 LDCM0441  CL50 HEK-293T C921(1.22); C927(1.10)  LDD1039  [1]
 LDCM0442  CL51 HEK-293T C921(1.04); C927(1.00)  LDD1040  [1]
 LDCM0443  CL52 HEK-293T C921(1.09); C927(0.99)  LDD1041  [1]
 LDCM0444  CL53 HEK-293T C921(1.08); C927(0.84)  LDD1042  [1]
 LDCM0445  CL54 HEK-293T C921(1.20); C927(1.03)  LDD1043  [1]
 LDCM0446  CL55 HEK-293T C921(0.95); C927(0.84)  LDD1044  [1]
 LDCM0447  CL56 HEK-293T C921(0.84); C927(1.05)  LDD1045  [1]
 LDCM0448  CL57 HEK-293T C921(1.01); C927(0.63)  LDD1046  [1]
 LDCM0449  CL58 HEK-293T C921(1.01); C927(0.73)  LDD1047  [1]
 LDCM0450  CL59 HEK-293T C921(1.13); C927(1.21)  LDD1048  [1]
 LDCM0452  CL60 HEK-293T C921(1.23); C927(1.46)  LDD1050  [1]
 LDCM0486  CL91 HEK-293T C927(1.44)  LDD1084  [1]
 LDCM0487  CL92 HEK-293T C927(1.25)  LDD1085  [1]
 LDCM0488  CL93 HEK-293T C927(1.12)  LDD1086  [1]
 LDCM0489  CL94 HEK-293T C927(1.50)  LDD1087  [1]
 LDCM0490  CL95 HEK-293T C927(1.87)  LDD1088  [1]
 LDCM0491  CL96 HEK-293T C927(1.76)  LDD1089  [1]
 LDCM0492  CL97 HEK-293T C927(1.53)  LDD1090  [1]
 LDCM0493  CL98 HEK-293T C927(1.23)  LDD1091  [1]
 LDCM0494  CL99 HEK-293T C927(1.44)  LDD1092  [1]
 LDCM0427  Fragment51 HEK-293T C927(1.18)  LDD1025  [1]
 LDCM0022  KB02 A2780 C203(2.55)  LDD2254  [4]
 LDCM0023  KB03 A2780 C203(4.06)  LDD2671  [4]
 LDCM0024  KB05 OV-90 C203(2.93)  LDD3380  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger protein RFP (TRIM27) TRIM/RBCC family P14373
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Fanconi anemia core complex-associated protein 24 (FAAP24) . Q9BTP7

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
3 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840