Details of the Target

General Information of Target

Target ID LDTP08418
Target Name RAS protein activator like-3 (RASAL3)
Gene Name RASAL3
Gene ID 64926
Synonyms
RAS protein activator like-3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDPPSPSRTSQTQPTATSPLTSYRWHTGGGGEKAAGGFRWGRFAGWGRALSHQEPMVSTQ
PAPRSIFRRVLSAPPKESRTSRLRLSKALWGRHKNPPPEPDPEPEQEAPELEPEPELEPP
TPQIPEAPTPNVPVWDIGGFTLLDGKLVLLGGEEEGPRRPRVGSASSEGSIHVAMGNFRD
PDRMPGKTEPETAGPNQVHNVRGLLKRLKEKKKARLEPRDGPPSALGSRESLATLSELDL
GAERDVRIWPLHPSLLGEPHCFQVTWTGGSRCFSCRSAAERDRWIEDLRRQFQPTQDNVE
REETWLSVWVHEAKGLPRAAAGAPGVRAELWLDGALLARTAPRAGPGQLFWAERFHFEAL
PPARRLSLRLRGLGPGSAVLGRVALALEELDAPRAPAAGLERWFPLLGAPAGAALRARIR
ARRLRVLPSERYKELAEFLTFHYARLCGALEPALPAQAKEELAAAMVRVLRATGRAQALV
TDLGTAELARCGGREALLFRENTLATKAIDEYMKLVAQDYLQETLGQVVRRLCASTEDCE
VDPSKCPASELPEHQARLRNSCEEVFETIIHSYDWFPAELGIVFSSWREACKERGSEVLG
PRLVCASLFLRLLCPAILAPSLFGLAPDHPAPGPARTLTLIAKVIQNLANRAPFGEKEAY
MGFMNSFLEEHGPAMQCFLDQVAMVDVDAAPSGYQGSGDLALQLAVLHAQLCTIFAELDQ
TTRDTLEPLPTILRAIEEGQPVLVSVPMRLPLPPAQVHSSLSAGEKPGFLAPRDLPKHTP
LISKSQSLRSVRRSESWARPRPDEERPLRRPRPVQRTQSVPVRRPARRRQSAGPWPRPKG
SLSMGPAPRARPWTRDSASLPRKPSVPWQRQMDQPQDRNQALGTHRPVNKLAELQCEVAA
LREEQKVLSRLVESLSTQIRALTEQQEQLRGQLQDLDSRLRAGSSEFDSEHNLTSNEGHS
LKNLEHRLNEMERTQAQLRDAVQSLQLSPRTRGSWSQPQPLKAPCLNGDTT
Target Bioclass
Other
Subcellular location
Cytoplasm
Function
Functions as a Ras GTPase-activating protein. Plays an important role in the expansion and functions of natural killer T (NKT) cells in the liver by negatively regulating RAS activity and the down-stream ERK signaling pathway.
Uniprot ID
Q86YV0
Ensemble ID
ENST00000343625.12
HGNC ID
HGNC:26129

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
BXPC3 SNV: p.R812W .
HCT116 SNV: p.Q876H .
HCT15 SNV: p.R327C .
IGR1 SNV: p.D873A .
IM95 Deletion: p.P120LfsTer89 .
JURKAT SNV: p.K33N Compound 10    Probe Info 
MCC26 Substitution: p.P122S .
MOLT4 SNV: p.R490H IA-alkyne    Probe Info 
OVCAR5 SNV: p.E795D .
REH SNV: p.R886Q DBIA    Probe Info 
SBC5 Deletion: p.R382AfsTer99 .
SKMEL2 SNV: p.A62T .
SKOV3 SNV: p.R215W .
SNU1 SNV: p.L205I .
TE4 SNV: p.E727V .
TOV21G Substitution: p.Q876K .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
HPAP
 Probe Info  		6.67  LDD0064  [1]
Alkyne-RA190
 Probe Info  		3.33  LDD0300  [2]
DBIA
 Probe Info  		C605(48.74); C546(44.85)  LDD0209  [3]
4-Iodoacetamidophenylacetylene
 Probe Info  		C896(0.00); C614(0.00); C546(0.00); C605(0.00)  LDD0038  [4]
IA-alkyne
 Probe Info  		C447(0.00); C614(0.00); C605(0.00); C896(0.00)  LDD0036  [4]
Lodoacetamide azide
 Probe Info  		C546(0.00); C896(0.00); C447(0.00); C614(0.00)  LDD0037  [4]
Compound 10
 Probe Info  		N.A.  LDD2216  [5]
Compound 11
 Probe Info  		N.A.  LDD2213  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C896(1.64); C1005(1.42)  LDD2187  [6]
 LDCM0572  Fragment10 Ramos C896(0.53); C1005(1.02)  LDD2189  [6]
 LDCM0573  Fragment11 Ramos C896(2.49)  LDD2190  [6]
 LDCM0574  Fragment12 Ramos C896(0.65); C1005(0.50)  LDD2191  [6]
 LDCM0575  Fragment13 Ramos C1005(0.63)  LDD2192  [6]
 LDCM0576  Fragment14 Ramos C1005(0.98)  LDD2193  [6]
 LDCM0579  Fragment20 Ramos C896(0.65); C1005(0.43)  LDD2194  [6]
 LDCM0580  Fragment21 Ramos C1005(0.74)  LDD2195  [6]
 LDCM0582  Fragment23 Ramos C1005(0.45)  LDD2196  [6]
 LDCM0586  Fragment28 Ramos C896(1.70); C1005(0.48)  LDD2198  [6]
 LDCM0588  Fragment30 Ramos C896(1.03); C1005(0.79)  LDD2199  [6]
 LDCM0590  Fragment32 Ramos C896(0.90)  LDD2201  [6]
 LDCM0468  Fragment33 Ramos C896(1.13)  LDD2202  [6]
 LDCM0596  Fragment38 Ramos C896(1.61); C1005(0.32)  LDD2203  [6]
 LDCM0610  Fragment52 Ramos C896(0.46); C1005(0.62)  LDD2204  [6]
 LDCM0569  Fragment7 Ramos C1005(0.99)  LDD2186  [6]
 LDCM0571  Fragment9 Ramos C896(0.77)  LDD2188  [6]
 LDCM0022  KB02 697 C546(2.06)  LDD2245  [7]
 LDCM0023  KB03 Jurkat C605(48.74); C546(44.85)  LDD0209  [3]
 LDCM0024  KB05 MOLM-13 C546(3.59)  LDD3333  [7]
 LDCM0014  Panhematin hPBMC 6.67  LDD0064  [1]
 LDCM0131  RA190 MM1.R 3.33  LDD0300  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein N-terminal glutamine amidohydrolase (NTAQ1) NTAQ1 family Q96HA8
Ubiquitin thioesterase ZRANB1 (ZRANB1) Peptidase C64 family Q9UGI0
5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2) CAMK Ser/Thr protein kinase family P54646
Dexamethasone-induced Ras-related protein 1 (RASD1) RasD family Q9Y272
Other
Click To Hide/Show 12 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Angiomotin-like protein 2 (AMOTL2) Angiomotin family Q9Y2J4
Apoptosis-stimulating of p53 protein 1 (PPP1R13B) ASPP family Q96KQ4
Cerebellar degeneration-related protein 2-like (CDR2L) CDR2 family Q86X02
Homer protein homolog 1 (HOMER1) Homer family Q86YM7
Keratin, type I cytoskeletal 27 (KRT27) Intermediate filament family Q7Z3Y8
KH domain-containing, RNA-binding, signal transduction-associated protein 3 (KHDRBS3) KHDRBS family O75525
U1 small nuclear ribonucleoprotein A (SNRPA) RRM U1 A/B'' family P09012
Brain-enriched guanylate kinase-associated protein (BEGAIN) . Q9BUH8
Coiled-coil domain-containing protein 102B (CCDC102B) . Q68D86
Differentially expressed in FDCP 6 homolog (DEF6) . Q9H4E7
PRKCA-binding protein (PICK1) . Q9NRD5
RAS protein activator like-3 (RASAL3) . Q86YV0

References

1 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
2 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.
3 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
4 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
5 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
6 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
7 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840