Details of the Target

General Information of Target

Target ID LDTP08405
Target Name Ankyrin repeat domain-containing protein 13B (ANKRD13B)
Gene Name ANKRD13B
Gene ID 124930
Synonyms
Ankyrin repeat domain-containing protein 13B
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MIPANASARKGPEGKYPLHYLVWHNRHRELEKEVRAGQVDIEQLDPRGRTPLHLATTLGH
LECARVLLAHGADVGRENRSGWTVLQEAVSTRDLELVQLVLRYRDYQRVVKRLAGIPVLL
EKLRKAQDFYVEMKWEFTSWVPLVSKICPSDTYKVWKSGQNLRVDTTLLGFDHMTWQRGN
RSFVFRGQDTSAVVMEIDHDRRVVYTETLALAGQDRELLLAAAQPTEEQVLSRLTAPVVT
TQLDTKNISFERNKTGILGWRSEKTEMVNGYEAKVYGASNVELITRTRTEHLSEQHKGKV
KGCKTPLQSFLGIAEQHGGPQNGTLITQTLSQANPTAITAEEYFNPNFELGNRDMGRPME
LTTKTQKFKAKLWLCEEHPLSLCEQVAPIIDLMAVSNALFAKLRDFITLRLPPGFPVKIE
IPIFHILNARITFGNLNGCDEPVPSVRGSPSSETPSPGSDSSSVSSSSSTTSCRGCEISP
ALFEAPRGYSMMGGQREAATRDDDDDLLQFAIQQSLLEAGSEYDQVTIWEALTNSKPGTH
PMSYEGRRQDRSAPPTPQRQPAPPASVPSPRPSSGPGSGGHVFRSYDEQLRLAMELSAQE
QEERRRRARQEEEELERILRLSLTEQ
Target Bioclass
Other
Subcellular location
Cell membrane
Function
Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Does not bind 'Lys-48'-linked ubiquitin. Positively regulates the internalization of ligand-activated EGFR by binding to the Ub moiety of ubiquitinated EGFR at the cell membrane.
Uniprot ID
Q86YJ7
Ensemble ID
ENST00000394859.8
HGNC ID
HGNC:26363

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A2780 SNV: p.A565V .
A375 SNV: p.P46L .
C4II SNV: p.Q224K .
HCC1143 SNV: p.D215H .
HCT116 Insertion: p.A565GfsTer158 .
HCT15 SNV: p.L30M; p.A88D .
HEP3B217 SNV: p.P568H .
JURKAT SNV: p.P225S .
LS123 Deletion: p.Q214GfsTer16 .
MOLT4 SNV: p.R47H .
NALM6 SNV: p.R548Ter .
NCIH1993 SNV: p.V109L .
RVH421 SNV: p.P117L .
TOV21G SNV: p.R286H .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C473(1.49)  LDD0812  [1]
IPM
 Probe Info  		N.A.  LDD2156  [2]
TFBX
 Probe Info  		N.A.  LDD0148  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C473(1.49)  LDD0812  [1]
 LDCM0215  AC10 HEK-293T C473(1.07)  LDD0813  [1]
 LDCM0226  AC11 HEK-293T C473(1.13)  LDD0824  [1]
 LDCM0237  AC12 HEK-293T C473(0.97)  LDD0835  [1]
 LDCM0259  AC14 HEK-293T C473(1.05)  LDD0857  [1]
 LDCM0270  AC15 HEK-293T C473(1.52)  LDD0868  [1]
 LDCM0276  AC17 HEK-293T C473(1.33)  LDD0874  [1]
 LDCM0277  AC18 HEK-293T C473(1.32)  LDD0875  [1]
 LDCM0278  AC19 HEK-293T C473(1.11)  LDD0876  [1]
 LDCM0279  AC2 HEK-293T C473(1.11)  LDD0877  [1]
 LDCM0280  AC20 HEK-293T C473(1.11)  LDD0878  [1]
 LDCM0281  AC21 HEK-293T C473(1.32)  LDD0879  [1]
 LDCM0282  AC22 HEK-293T C473(1.10)  LDD0880  [1]
 LDCM0283  AC23 HEK-293T C473(1.17)  LDD0881  [1]
 LDCM0284  AC24 HEK-293T C473(1.05)  LDD0882  [1]
 LDCM0285  AC25 HEK-293T C473(1.22)  LDD0883  [1]
 LDCM0286  AC26 HEK-293T C473(1.11)  LDD0884  [1]
 LDCM0287  AC27 HEK-293T C473(1.24)  LDD0885  [1]
 LDCM0288  AC28 HEK-293T C473(1.27)  LDD0886  [1]
 LDCM0289  AC29 HEK-293T C473(1.20)  LDD0887  [1]
 LDCM0290  AC3 HEK-293T C473(1.01)  LDD0888  [1]
 LDCM0291  AC30 HEK-293T C473(1.22)  LDD0889  [1]
 LDCM0292  AC31 HEK-293T C473(1.14)  LDD0890  [1]
 LDCM0293  AC32 HEK-293T C473(1.30)  LDD0891  [1]
 LDCM0294  AC33 HEK-293T C473(1.30)  LDD0892  [1]
 LDCM0295  AC34 HEK-293T C473(1.31)  LDD0893  [1]
 LDCM0301  AC4 HEK-293T C473(1.00)  LDD0899  [1]
 LDCM0312  AC5 HEK-293T C473(1.14)  LDD0910  [1]
 LDCM0320  AC57 HEK-293T C473(1.03)  LDD0918  [1]
 LDCM0321  AC58 HEK-293T C473(0.93)  LDD0919  [1]
 LDCM0322  AC59 HEK-293T C473(0.99)  LDD0920  [1]
 LDCM0323  AC6 HEK-293T C473(1.54)  LDD0921  [1]
 LDCM0324  AC60 HEK-293T C473(1.80)  LDD0922  [1]
 LDCM0325  AC61 HEK-293T C473(1.01)  LDD0923  [1]
 LDCM0326  AC62 HEK-293T C473(0.91)  LDD0924  [1]
 LDCM0327  AC63 HEK-293T C473(0.97)  LDD0925  [1]
 LDCM0328  AC64 HEK-293T C473(0.93)  LDD0926  [1]
 LDCM0329  AC65 HEK-293T C473(0.97)  LDD0927  [1]
 LDCM0330  AC66 HEK-293T C473(0.96)  LDD0928  [1]
 LDCM0331  AC67 HEK-293T C473(0.99)  LDD0929  [1]
 LDCM0334  AC7 HEK-293T C473(1.12)  LDD0932  [1]
 LDCM0345  AC8 HEK-293T C473(1.38)  LDD0943  [1]
 LDCM0349  AC83 HEK-293T C473(1.09)  LDD0947  [1]
 LDCM0350  AC84 HEK-293T C473(1.07)  LDD0948  [1]
 LDCM0351  AC85 HEK-293T C473(1.02)  LDD0949  [1]
 LDCM0352  AC86 HEK-293T C473(0.93)  LDD0950  [1]
 LDCM0353  AC87 HEK-293T C473(0.92)  LDD0951  [1]
 LDCM0354  AC88 HEK-293T C473(0.95)  LDD0952  [1]
 LDCM0355  AC89 HEK-293T C473(1.04)  LDD0953  [1]
 LDCM0357  AC90 HEK-293T C473(1.12)  LDD0955  [1]
 LDCM0358  AC91 HEK-293T C473(0.99)  LDD0956  [1]
 LDCM0359  AC92 HEK-293T C473(0.98)  LDD0957  [1]
 LDCM0360  AC93 HEK-293T C473(0.99)  LDD0958  [1]
 LDCM0361  AC94 HEK-293T C473(0.90)  LDD0959  [1]
 LDCM0362  AC95 HEK-293T C473(1.05)  LDD0960  [1]
 LDCM0363  AC96 HEK-293T C473(0.88)  LDD0961  [1]
 LDCM0364  AC97 HEK-293T C473(1.27)  LDD0962  [1]
 LDCM0248  AKOS034007472 HEK-293T C473(0.97)  LDD0846  [1]
 LDCM0356  AKOS034007680 HEK-293T C473(1.19)  LDD0954  [1]
 LDCM0275  AKOS034007705 HEK-293T C473(1.25)  LDD0873  [1]
 LDCM0367  CL1 HEK-293T C473(1.16)  LDD0965  [1]
 LDCM0368  CL10 HEK-293T C473(1.15)  LDD0966  [1]
 LDCM0369  CL100 HEK-293T C473(1.05)  LDD0967  [1]
 LDCM0370  CL101 HEK-293T C473(1.16)  LDD0968  [1]
 LDCM0371  CL102 HEK-293T C473(1.14)  LDD0969  [1]
 LDCM0372  CL103 HEK-293T C473(1.23)  LDD0970  [1]
 LDCM0373  CL104 HEK-293T C473(1.33)  LDD0971  [1]
 LDCM0374  CL105 HEK-293T C473(1.09)  LDD0972  [1]
 LDCM0375  CL106 HEK-293T C473(1.03)  LDD0973  [1]
 LDCM0376  CL107 HEK-293T C473(1.20)  LDD0974  [1]
 LDCM0377  CL108 HEK-293T C473(1.15)  LDD0975  [1]
 LDCM0378  CL109 HEK-293T C473(1.04)  LDD0976  [1]
 LDCM0379  CL11 HEK-293T C473(1.03)  LDD0977  [1]
 LDCM0380  CL110 HEK-293T C473(1.05)  LDD0978  [1]
 LDCM0381  CL111 HEK-293T C473(1.02)  LDD0979  [1]
 LDCM0382  CL112 HEK-293T C473(1.12)  LDD0980  [1]
 LDCM0383  CL113 HEK-293T C473(1.15)  LDD0981  [1]
 LDCM0384  CL114 HEK-293T C473(1.20)  LDD0982  [1]
 LDCM0385  CL115 HEK-293T C473(1.34)  LDD0983  [1]
 LDCM0386  CL116 HEK-293T C473(1.27)  LDD0984  [1]
 LDCM0390  CL12 HEK-293T C473(0.97)  LDD0988  [1]
 LDCM0396  CL125 HEK-293T C473(0.98)  LDD0994  [1]
 LDCM0397  CL126 HEK-293T C473(0.93)  LDD0995  [1]
 LDCM0398  CL127 HEK-293T C473(0.93)  LDD0996  [1]
 LDCM0399  CL128 HEK-293T C473(0.97)  LDD0997  [1]
 LDCM0400  CL13 HEK-293T C473(0.93)  LDD0998  [1]
 LDCM0401  CL14 HEK-293T C473(0.88)  LDD0999  [1]
 LDCM0402  CL15 HEK-293T C473(1.01)  LDD1000  [1]
 LDCM0403  CL16 HEK-293T C473(1.18)  LDD1001  [1]
 LDCM0404  CL17 HEK-293T C473(0.96)  LDD1002  [1]
 LDCM0405  CL18 HEK-293T C473(1.07)  LDD1003  [1]
 LDCM0406  CL19 HEK-293T C473(1.06)  LDD1004  [1]
 LDCM0407  CL2 HEK-293T C473(0.90)  LDD1005  [1]
 LDCM0408  CL20 HEK-293T C473(1.04)  LDD1006  [1]
 LDCM0409  CL21 HEK-293T C473(0.84)  LDD1007  [1]
 LDCM0410  CL22 HEK-293T C473(0.88)  LDD1008  [1]
 LDCM0411  CL23 HEK-293T C473(0.79)  LDD1009  [1]
 LDCM0412  CL24 HEK-293T C473(0.93)  LDD1010  [1]
 LDCM0413  CL25 HEK-293T C473(0.88)  LDD1011  [1]
 LDCM0414  CL26 HEK-293T C473(0.82)  LDD1012  [1]
 LDCM0415  CL27 HEK-293T C473(0.92)  LDD1013  [1]
 LDCM0416  CL28 HEK-293T C473(0.76)  LDD1014  [1]
 LDCM0417  CL29 HEK-293T C473(0.86)  LDD1015  [1]
 LDCM0418  CL3 HEK-293T C473(0.98)  LDD1016  [1]
 LDCM0419  CL30 HEK-293T C473(0.97)  LDD1017  [1]
 LDCM0429  CL4 HEK-293T C473(0.96)  LDD1027  [1]
 LDCM0436  CL46 HEK-293T C473(1.28)  LDD1034  [1]
 LDCM0437  CL47 HEK-293T C473(1.08)  LDD1035  [1]
 LDCM0438  CL48 HEK-293T C473(1.06)  LDD1036  [1]
 LDCM0439  CL49 HEK-293T C473(1.04)  LDD1037  [1]
 LDCM0440  CL5 HEK-293T C473(0.93)  LDD1038  [1]
 LDCM0441  CL50 HEK-293T C473(1.06)  LDD1039  [1]
 LDCM0442  CL51 HEK-293T C473(0.97)  LDD1040  [1]
 LDCM0443  CL52 HEK-293T C473(1.03)  LDD1041  [1]
 LDCM0444  CL53 HEK-293T C473(0.99)  LDD1042  [1]
 LDCM0445  CL54 HEK-293T C473(0.93)  LDD1043  [1]
 LDCM0446  CL55 HEK-293T C473(1.13)  LDD1044  [1]
 LDCM0447  CL56 HEK-293T C473(0.92)  LDD1045  [1]
 LDCM0448  CL57 HEK-293T C473(1.02)  LDD1046  [1]
 LDCM0449  CL58 HEK-293T C473(0.98)  LDD1047  [1]
 LDCM0450  CL59 HEK-293T C473(1.09)  LDD1048  [1]
 LDCM0451  CL6 HEK-293T C473(0.89)  LDD1049  [1]
 LDCM0452  CL60 HEK-293T C473(1.11)  LDD1050  [1]
 LDCM0453  CL61 HEK-293T C473(1.24)  LDD1051  [1]
 LDCM0454  CL62 HEK-293T C473(1.07)  LDD1052  [1]
 LDCM0455  CL63 HEK-293T C473(1.22)  LDD1053  [1]
 LDCM0456  CL64 HEK-293T C473(1.16)  LDD1054  [1]
 LDCM0457  CL65 HEK-293T C473(1.15)  LDD1055  [1]
 LDCM0458  CL66 HEK-293T C473(1.03)  LDD1056  [1]
 LDCM0459  CL67 HEK-293T C473(0.95)  LDD1057  [1]
 LDCM0460  CL68 HEK-293T C473(0.89)  LDD1058  [1]
 LDCM0461  CL69 HEK-293T C473(1.02)  LDD1059  [1]
 LDCM0462  CL7 HEK-293T C473(0.92)  LDD1060  [1]
 LDCM0463  CL70 HEK-293T C473(1.01)  LDD1061  [1]
 LDCM0464  CL71 HEK-293T C473(0.95)  LDD1062  [1]
 LDCM0465  CL72 HEK-293T C473(1.18)  LDD1063  [1]
 LDCM0466  CL73 HEK-293T C473(0.90)  LDD1064  [1]
 LDCM0467  CL74 HEK-293T C473(1.11)  LDD1065  [1]
 LDCM0473  CL8 HEK-293T C473(1.28)  LDD1071  [1]
 LDCM0484  CL9 HEK-293T C473(1.12)  LDD1082  [1]
 LDCM0486  CL91 HEK-293T C473(1.32)  LDD1084  [1]
 LDCM0487  CL92 HEK-293T C473(0.96)  LDD1085  [1]
 LDCM0488  CL93 HEK-293T C473(1.05)  LDD1086  [1]
 LDCM0489  CL94 HEK-293T C473(1.15)  LDD1087  [1]
 LDCM0490  CL95 HEK-293T C473(1.33)  LDD1088  [1]
 LDCM0491  CL96 HEK-293T C473(1.15)  LDD1089  [1]
 LDCM0492  CL97 HEK-293T C473(1.12)  LDD1090  [1]
 LDCM0493  CL98 HEK-293T C473(1.00)  LDD1091  [1]
 LDCM0494  CL99 HEK-293T C473(1.11)  LDD1092  [1]
 LDCM0468  Fragment33 HEK-293T C473(1.02)  LDD1066  [1]

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255