Details of the Target

General Information of Target

Target ID LDTP08337
Target Name Vasculin (GPBP1)
Gene Name GPBP1
Gene ID 65056
Synonyms
GPBP; SSH6; Vasculin; GC-rich promoter-binding protein 1; Vascular wall-linked protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAQHDFAPAWLNFPTPPSSTKSSLNFEKHSENFAWTENRYDVNRRRHNSSDGFDSAIGRP
NGGNFGRKEKNGWRTHGRNGTENINHRGGYHGGSSRSRSSIFHAGKSQGLHENNIPDNET
GRKEDKRERKQFEAEDFPSLNPEYEREPNHNKSLAAGVWEYPPNPKSRAPRMLVIKKGNT
KDLQLSGFPVVGNLPSQPVKNGTGPSVYKGLVPKPAAPPTKPTQWKSQTKENKVGTSFPH
ESTFGVGNFNAFKSTAKNFSPSTNSVKECNRSNSSSPVDKLNQQPRLTKLTRMRTDKKSE
FLKALKRDRVEEEHEDESRAGSEKDDDSFNLHNSNSTHQERDINRNFDENEIPQENGNAS
VISQQIIRSSTFPQTDVLSSSLEAEHRLLKEMGWQEDSENDETCAPLTEDEMREFQVISE
QLQKNGLRKNGILKNGLICDFKFGPWKNSTFKPTTENDDTETSSSDTSDDDDV
Target Bioclass
Other
Family
Vasculin family
Subcellular location
Nucleus
Function Functions as a GC-rich promoter-specific transactivating transcription factor.
Uniprot ID
Q86WP2
Ensemble ID
ENST00000264779.6
HGNC ID
HGNC:29520

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HS936T SNV: p.E391K .
HT115 SNV: p.K106N .
LS180 SNV: p.W446R .
MOLT4 SNV: p.R129H .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info  		C269(1.60)  LDD2182  [1]
DBIA
 Probe Info  		C439(0.88)  LDD1507  [2]
IPM
 Probe Info  		N.A.  LDD0025  [3]
NAIA_4
 Probe Info  		N.A.  LDD2226  [4]
TFBX
 Probe Info  		N.A.  LDD0148  [3]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C439(0.88)  LDD1507  [2]
 LDCM0270  AC15 HEK-293T C439(1.07)  LDD1513  [2]
 LDCM0276  AC17 HEK-293T C439(0.97)  LDD1515  [2]
 LDCM0281  AC21 HEK-293T C439(1.05)  LDD1520  [2]
 LDCM0283  AC23 HEK-293T C439(0.96)  LDD1522  [2]
 LDCM0284  AC24 HEK-293T C439(0.92)  LDD1523  [2]
 LDCM0285  AC25 HEK-293T C439(0.92)  LDD1524  [2]
 LDCM0289  AC29 HEK-293T C439(1.07)  LDD1528  [2]
 LDCM0292  AC31 HEK-293T C439(1.05)  LDD1531  [2]
 LDCM0293  AC32 HEK-293T C439(0.97)  LDD1532  [2]
 LDCM0294  AC33 HEK-293T C439(0.98)  LDD1533  [2]
 LDCM0298  AC37 HEK-293T C439(1.12)  LDD1537  [2]
 LDCM0300  AC39 HEK-293T C439(1.05)  LDD1539  [2]
 LDCM0302  AC40 HEK-293T C439(1.01)  LDD1541  [2]
 LDCM0303  AC41 HEK-293T C439(0.90)  LDD1542  [2]
 LDCM0307  AC45 HEK-293T C439(1.13)  LDD1546  [2]
 LDCM0309  AC47 HEK-293T C439(1.01)  LDD1548  [2]
 LDCM0310  AC48 HEK-293T C439(1.05)  LDD1549  [2]
 LDCM0311  AC49 HEK-293T C439(0.99)  LDD1550  [2]
 LDCM0312  AC5 HEK-293T C439(1.09)  LDD1551  [2]
 LDCM0316  AC53 HEK-293T C439(1.07)  LDD1555  [2]
 LDCM0318  AC55 HEK-293T C439(1.09)  LDD1557  [2]
 LDCM0319  AC56 HEK-293T C439(0.97)  LDD1558  [2]
 LDCM0320  AC57 HEK-293T C439(0.92)  LDD1559  [2]
 LDCM0325  AC61 HEK-293T C439(1.13)  LDD1564  [2]
 LDCM0327  AC63 HEK-293T C439(1.09)  LDD1566  [2]
 LDCM0328  AC64 HEK-293T C439(0.97)  LDD1567  [2]
 LDCM0334  AC7 HEK-293T C439(0.84)  LDD1568  [2]
 LDCM0345  AC8 HEK-293T C439(0.95)  LDD1569  [2]
 LDCM0248  AKOS034007472 HEK-293T C439(1.04)  LDD1511  [2]
 LDCM0356  AKOS034007680 HEK-293T C439(0.92)  LDD1570  [2]
 LDCM0275  AKOS034007705 HEK-293T C439(1.01)  LDD1514  [2]
 LDCM0371  CL102 HEK-293T C439(1.11)  LDD1575  [2]
 LDCM0375  CL106 HEK-293T C439(1.09)  LDD1579  [2]
 LDCM0379  CL11 HEK-293T C439(0.99)  LDD1583  [2]
 LDCM0380  CL110 HEK-293T C439(1.08)  LDD1584  [2]
 LDCM0384  CL114 HEK-293T C439(1.01)  LDD1588  [2]
 LDCM0388  CL118 HEK-293T C439(1.11)  LDD1592  [2]
 LDCM0390  CL12 HEK-293T C439(1.11)  LDD1594  [2]
 LDCM0393  CL122 HEK-293T C439(1.10)  LDD1597  [2]
 LDCM0397  CL126 HEK-293T C439(1.18)  LDD1601  [2]
 LDCM0401  CL14 HEK-293T C439(1.05)  LDD1605  [2]
 LDCM0404  CL17 HEK-293T C439(1.02)  LDD1608  [2]
 LDCM0407  CL2 HEK-293T C439(1.09)  LDD1611  [2]
 LDCM0409  CL21 HEK-293T C439(1.06)  LDD1613  [2]
 LDCM0411  CL23 HEK-293T C439(1.03)  LDD1615  [2]
 LDCM0412  CL24 HEK-293T C439(0.98)  LDD1616  [2]
 LDCM0414  CL26 HEK-293T C439(1.10)  LDD1618  [2]
 LDCM0417  CL29 HEK-293T C439(1.02)  LDD1621  [2]
 LDCM0422  CL33 HEK-293T C439(1.09)  LDD1626  [2]
 LDCM0424  CL35 HEK-293T C439(1.03)  LDD1628  [2]
 LDCM0425  CL36 HEK-293T C439(0.93)  LDD1629  [2]
 LDCM0431  CL41 HEK-293T C439(0.93)  LDD1635  [2]
 LDCM0435  CL45 HEK-293T C439(1.04)  LDD1639  [2]
 LDCM0437  CL47 HEK-293T C439(0.95)  LDD1641  [2]
 LDCM0438  CL48 HEK-293T C439(0.99)  LDD1642  [2]
 LDCM0440  CL5 HEK-293T C439(0.95)  LDD1644  [2]
 LDCM0441  CL50 HEK-293T C439(1.06)  LDD1645  [2]
 LDCM0444  CL53 HEK-293T C439(0.88)  LDD1647  [2]
 LDCM0448  CL57 HEK-293T C439(1.27)  LDD1651  [2]
 LDCM0450  CL59 HEK-293T C439(1.23)  LDD1653  [2]
 LDCM0452  CL60 HEK-293T C439(1.08)  LDD1655  [2]
 LDCM0454  CL62 HEK-293T C439(1.11)  LDD1657  [2]
 LDCM0457  CL65 HEK-293T C439(0.98)  LDD1660  [2]
 LDCM0461  CL69 HEK-293T C439(1.13)  LDD1664  [2]
 LDCM0464  CL71 HEK-293T C439(1.07)  LDD1667  [2]
 LDCM0465  CL72 HEK-293T C439(1.02)  LDD1668  [2]
 LDCM0467  CL74 HEK-293T C439(1.03)  LDD1670  [2]
 LDCM0470  CL77 HEK-293T C439(0.90)  LDD1673  [2]
 LDCM0475  CL81 HEK-293T C439(1.14)  LDD1678  [2]
 LDCM0477  CL83 HEK-293T C439(1.09)  LDD1680  [2]
 LDCM0478  CL84 HEK-293T C439(1.04)  LDD1681  [2]
 LDCM0480  CL86 HEK-293T C439(1.01)  LDD1683  [2]
 LDCM0483  CL89 HEK-293T C439(0.84)  LDD1686  [2]
 LDCM0484  CL9 HEK-293T C439(1.10)  LDD1687  [2]
 LDCM0488  CL93 HEK-293T C439(1.08)  LDD1691  [2]
 LDCM0490  CL95 HEK-293T C439(1.05)  LDD1693  [2]
 LDCM0491  CL96 HEK-293T C439(1.14)  LDD1694  [2]
 LDCM0493  CL98 HEK-293T C439(1.00)  LDD1696  [2]
 LDCM0625  F8 Ramos C269(1.82)  LDD2187  [1]
 LDCM0572  Fragment10 Ramos C269(3.07)  LDD2189  [1]
 LDCM0573  Fragment11 Ramos C269(5.97)  LDD2190  [1]
 LDCM0574  Fragment12 Ramos C269(0.71)  LDD2191  [1]
 LDCM0575  Fragment13 Ramos C269(0.74)  LDD2192  [1]
 LDCM0576  Fragment14 Ramos C269(0.58)  LDD2193  [1]
 LDCM0579  Fragment20 Ramos C269(0.71)  LDD2194  [1]
 LDCM0580  Fragment21 Ramos C269(0.79)  LDD2195  [1]
 LDCM0582  Fragment23 Ramos C269(0.76)  LDD2196  [1]
 LDCM0578  Fragment27 Ramos C269(0.77)  LDD2197  [1]
 LDCM0586  Fragment28 Ramos C269(0.88)  LDD2198  [1]
 LDCM0588  Fragment30 Ramos C269(0.92)  LDD2199  [1]
 LDCM0589  Fragment31 Ramos C269(0.78)  LDD2200  [1]
 LDCM0590  Fragment32 Ramos C269(1.35)  LDD2201  [1]
 LDCM0468  Fragment33 Ramos C269(0.85)  LDD2202  [1]
 LDCM0596  Fragment38 Ramos C269(0.78)  LDD2203  [1]
 LDCM0566  Fragment4 Ramos C269(1.18)  LDD2184  [1]
 LDCM0427  Fragment51 HEK-293T C439(1.01)  LDD1631  [2]
 LDCM0610  Fragment52 Ramos C269(0.81)  LDD2204  [1]
 LDCM0614  Fragment56 Ramos C269(1.34)  LDD2205  [1]
 LDCM0569  Fragment7 Ramos C269(1.84)  LDD2186  [1]
 LDCM0571  Fragment9 Ramos C269(1.03)  LDD2188  [1]
 LDCM0022  KB02 Ramos C269(1.60)  LDD2182  [1]
 LDCM0023  KB03 Ramos C269(1.08)  LDD2183  [1]
 LDCM0024  KB05 Ramos C269(1.09)  LDD2185  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Glutamine--tRNA ligase (QARS1) Class-I aminoacyl-tRNA synthetase family P47897
Proteasome subunit beta type-1 (PSMB1) Peptidase T1B family P20618
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Nuclear RNA export factor 1 (NXF1) NXF family Q9UBU9
Small ribosomal subunit protein RACK1 (RACK1) WD repeat G protein beta family P63244
Other
Click To Hide/Show 7 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein FAM161A (FAM161A) FAM161 family Q3B820
Pre-mRNA-splicing factor 18 (PRPF18) PRP18 family Q99633
Fibronectin type III domain-containing protein 11 (FNDC11) . Q9BVV2
Heat shock factor 2-binding protein (HSF2BP) . O75031
Microspherule protein 1 (MCRS1) . Q96EZ8
Mortality factor 4-like protein 2 (MORF4L2) . Q15014
Pleckstrin homology domain-containing family F member 2 (PLEKHF2) . Q9H8W4

References

1 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004