Details of the Target

General Information of Target

Target ID LDTP07942
Target Name Cytochrome b5 reductase 4 (CYB5R4)
Gene Name CYB5R4
Gene ID 51167
Synonyms
NCB5OR; Cytochrome b5 reductase 4; EC 1.6.2.2; Flavohemoprotein b5/b5R; b5+b5R; N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein; cb5/cb5R
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLNVPSQSFPAPRSQQRVASGGRSKVPLKQGRSLMDWIRLTKSGKDLTGLKGRLIEVTEE
ELKKHNKKDDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGSDGTELFDQVHRWVNYESM
LKECLVGRMAIKPAVLKDYREEEKKVLNGMLPKSQVTDTLAKEGPSYPSYDWFQTDSLVT
IAIYTKQKDINLDSIIVDHQNDSFRAETIIKDCLYLIHIGLSHEVQEDFSVRVVESVGKI
EIVLQKKENTSWDFLGHPLKNHNSLIPRKDTGLYYRKCQLISKEDVTHDTRLFCLMLPPS
THLQVPIGQHVYLKLPITGTEIVKPYTPVSGSLLSEFKEPVLPNNKYIYFLIKIYPTGLF
TPELDRLQIGDFVSVSSPEGNFKISKFQELEDLFLLAAGTGFTPMVKILNYALTDIPSLR
KVKLMFFNKTEDDIIWRSQLEKLAFKDKRLDVEFVLSAPISEWNGKQGHISPALLSEFLK
RNLDKSKVLVCICGPVPFTEQGVRLLHDLNFSKNEIHSFTA
Target Bioclass
Enzyme
Family
Flavoprotein pyridine nucleotide cytochrome reductase family
Subcellular location
Endoplasmic reticulum
Function
NADH-cytochrome b5 reductase involved in endoplasmic reticulum stress response pathway. Plays a critical role in protecting pancreatic beta-cells against oxidant stress, possibly by protecting the cell from excess buildup of reactive oxygen species (ROS). Reduces a variety of substrates in vitro, such as cytochrome c, feericyanide and methemoglobin.
Uniprot ID
Q7L1T6
Ensemble ID
ENST00000369681.10
HGNC ID
HGNC:20147

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
BTD
 Probe Info  		C278(0.63)  LDD2132  [1]
IPM
 Probe Info  		C278(2.85)  LDD1702  [1]
DBIA
 Probe Info  		C213(0.97)  LDD1508  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0539  3-(4-Isopropylpiperazin-1-yl)-3-oxopropanenitrile MDA-MB-231 C278(0.63)  LDD2132  [1]
 LDCM0215  AC10 HEK-293T C213(0.97)  LDD1508  [2]
 LDCM0226  AC11 HEK-293T C213(0.93)  LDD1509  [2]
 LDCM0237  AC12 HEK-293T C213(1.23)  LDD1510  [2]
 LDCM0270  AC15 HEK-293T C213(1.06)  LDD1513  [2]
 LDCM0277  AC18 HEK-293T C213(0.99)  LDD1516  [2]
 LDCM0278  AC19 HEK-293T C213(1.07)  LDD1517  [2]
 LDCM0279  AC2 HEK-293T C213(1.05)  LDD1518  [2]
 LDCM0280  AC20 HEK-293T C213(1.13)  LDD1519  [2]
 LDCM0283  AC23 HEK-293T C213(1.08)  LDD1522  [2]
 LDCM0286  AC26 HEK-293T C213(1.12)  LDD1525  [2]
 LDCM0287  AC27 HEK-293T C213(1.10)  LDD1526  [2]
 LDCM0288  AC28 HEK-293T C213(1.01)  LDD1527  [2]
 LDCM0290  AC3 HEK-293T C213(1.01)  LDD1529  [2]
 LDCM0292  AC31 HEK-293T C213(0.91)  LDD1531  [2]
 LDCM0295  AC34 HEK-293T C213(1.06)  LDD1534  [2]
 LDCM0296  AC35 HEK-293T C213(1.11)  LDD1535  [2]
 LDCM0297  AC36 HEK-293T C213(1.01)  LDD1536  [2]
 LDCM0300  AC39 HEK-293T C213(0.93)  LDD1539  [2]
 LDCM0301  AC4 HEK-293T C213(1.18)  LDD1540  [2]
 LDCM0304  AC42 HEK-293T C213(1.01)  LDD1543  [2]
 LDCM0305  AC43 HEK-293T C213(1.07)  LDD1544  [2]
 LDCM0306  AC44 HEK-293T C213(1.05)  LDD1545  [2]
 LDCM0309  AC47 HEK-293T C213(0.98)  LDD1548  [2]
 LDCM0313  AC50 HEK-293T C213(1.01)  LDD1552  [2]
 LDCM0314  AC51 HEK-293T C213(1.03)  LDD1553  [2]
 LDCM0315  AC52 HEK-293T C213(1.23)  LDD1554  [2]
 LDCM0318  AC55 HEK-293T C213(1.15)  LDD1557  [2]
 LDCM0321  AC58 HEK-293T C213(0.97)  LDD1560  [2]
 LDCM0322  AC59 HEK-293T C213(0.99)  LDD1561  [2]
 LDCM0324  AC60 HEK-293T C213(1.32)  LDD1563  [2]
 LDCM0327  AC63 HEK-293T C213(0.89)  LDD1566  [2]
 LDCM0334  AC7 HEK-293T C213(1.01)  LDD1568  [2]
 LDCM0367  CL1 HEK-293T C213(1.02)  LDD1571  [2]
 LDCM0369  CL100 HEK-293T C213(1.04)  LDD1573  [2]
 LDCM0370  CL101 HEK-293T C213(1.19)  LDD1574  [2]
 LDCM0371  CL102 HEK-293T C213(0.80)  LDD1575  [2]
 LDCM0373  CL104 HEK-293T C213(1.07)  LDD1577  [2]
 LDCM0374  CL105 HEK-293T C213(1.07)  LDD1578  [2]
 LDCM0375  CL106 HEK-293T C213(1.11)  LDD1579  [2]
 LDCM0377  CL108 HEK-293T C213(1.10)  LDD1581  [2]
 LDCM0378  CL109 HEK-293T C213(1.13)  LDD1582  [2]
 LDCM0379  CL11 HEK-293T C213(1.09)  LDD1583  [2]
 LDCM0380  CL110 HEK-293T C213(1.07)  LDD1584  [2]
 LDCM0382  CL112 HEK-293T C213(1.01)  LDD1586  [2]
 LDCM0383  CL113 HEK-293T C213(1.03)  LDD1587  [2]
 LDCM0384  CL114 HEK-293T C213(0.90)  LDD1588  [2]
 LDCM0386  CL116 HEK-293T C213(0.92)  LDD1590  [2]
 LDCM0387  CL117 HEK-293T C213(1.08)  LDD1591  [2]
 LDCM0388  CL118 HEK-293T C213(1.18)  LDD1592  [2]
 LDCM0391  CL120 HEK-293T C213(1.13)  LDD1595  [2]
 LDCM0392  CL121 HEK-293T C213(0.95)  LDD1596  [2]
 LDCM0393  CL122 HEK-293T C213(1.01)  LDD1597  [2]
 LDCM0395  CL124 HEK-293T C213(0.99)  LDD1599  [2]
 LDCM0396  CL125 HEK-293T C213(1.06)  LDD1600  [2]
 LDCM0397  CL126 HEK-293T C213(1.35)  LDD1601  [2]
 LDCM0399  CL128 HEK-293T C213(1.05)  LDD1603  [2]
 LDCM0400  CL13 HEK-293T C213(0.98)  LDD1604  [2]
 LDCM0401  CL14 HEK-293T C213(1.04)  LDD1605  [2]
 LDCM0403  CL16 HEK-293T C213(0.97)  LDD1607  [2]
 LDCM0405  CL18 HEK-293T C213(1.02)  LDD1609  [2]
 LDCM0406  CL19 HEK-293T C213(1.09)  LDD1610  [2]
 LDCM0407  CL2 HEK-293T C213(0.89)  LDD1611  [2]
 LDCM0408  CL20 HEK-293T C213(1.11)  LDD1612  [2]
 LDCM0411  CL23 HEK-293T C213(1.39)  LDD1615  [2]
 LDCM0413  CL25 HEK-293T C213(0.81)  LDD1617  [2]
 LDCM0414  CL26 HEK-293T C213(1.00)  LDD1618  [2]
 LDCM0416  CL28 HEK-293T C213(1.03)  LDD1620  [2]
 LDCM0419  CL30 HEK-293T C213(1.07)  LDD1623  [2]
 LDCM0420  CL31 HEK-293T C213(1.08)  LDD1624  [2]
 LDCM0421  CL32 HEK-293T C213(0.94)  LDD1625  [2]
 LDCM0424  CL35 HEK-293T C213(1.35)  LDD1628  [2]
 LDCM0426  CL37 HEK-293T C213(0.95)  LDD1630  [2]
 LDCM0429  CL4 HEK-293T C213(1.05)  LDD1633  [2]
 LDCM0430  CL40 HEK-293T C213(1.02)  LDD1634  [2]
 LDCM0432  CL42 HEK-293T C213(1.13)  LDD1636  [2]
 LDCM0433  CL43 HEK-293T C213(1.08)  LDD1637  [2]
 LDCM0434  CL44 HEK-293T C213(1.12)  LDD1638  [2]
 LDCM0437  CL47 HEK-293T C213(1.57)  LDD1641  [2]
 LDCM0439  CL49 HEK-293T C213(1.06)  LDD1643  [2]
 LDCM0441  CL50 HEK-293T C213(1.09)  LDD1645  [2]
 LDCM0443  CL52 HEK-293T C213(1.05)  LDD1646  [2]
 LDCM0445  CL54 HEK-293T C213(0.96)  LDD1648  [2]
 LDCM0446  CL55 HEK-293T C213(1.17)  LDD1649  [2]
 LDCM0447  CL56 HEK-293T C213(1.26)  LDD1650  [2]
 LDCM0450  CL59 HEK-293T C213(0.33)  LDD1653  [2]
 LDCM0451  CL6 HEK-293T C213(0.82)  LDD1654  [2]
 LDCM0453  CL61 HEK-293T C213(1.02)  LDD1656  [2]
 LDCM0454  CL62 HEK-293T C213(1.01)  LDD1657  [2]
 LDCM0456  CL64 HEK-293T C213(1.03)  LDD1659  [2]
 LDCM0458  CL66 HEK-293T C213(1.00)  LDD1661  [2]
 LDCM0459  CL67 HEK-293T C213(1.16)  LDD1662  [2]
 LDCM0460  CL68 HEK-293T C213(1.13)  LDD1663  [2]
 LDCM0462  CL7 HEK-293T C213(1.24)  LDD1665  [2]
 LDCM0464  CL71 HEK-293T C213(1.27)  LDD1667  [2]
 LDCM0466  CL73 HEK-293T C213(0.80)  LDD1669  [2]
 LDCM0467  CL74 HEK-293T C213(1.17)  LDD1670  [2]
 LDCM0469  CL76 HEK-293T C213(0.92)  LDD1672  [2]
 LDCM0471  CL78 HEK-293T C213(1.03)  LDD1674  [2]
 LDCM0472  CL79 HEK-293T C213(0.95)  LDD1675  [2]
 LDCM0473  CL8 HEK-293T C213(0.87)  LDD1676  [2]
 LDCM0474  CL80 HEK-293T C213(1.22)  LDD1677  [2]
 LDCM0477  CL83 HEK-293T C213(1.35)  LDD1680  [2]
 LDCM0479  CL85 HEK-293T C213(0.83)  LDD1682  [2]
 LDCM0480  CL86 HEK-293T C213(0.99)  LDD1683  [2]
 LDCM0482  CL88 HEK-293T C213(1.13)  LDD1685  [2]
 LDCM0485  CL90 HEK-293T C213(0.87)  LDD1688  [2]
 LDCM0486  CL91 HEK-293T C213(1.13)  LDD1689  [2]
 LDCM0487  CL92 HEK-293T C213(1.06)  LDD1690  [2]
 LDCM0490  CL95 HEK-293T C213(1.13)  LDD1693  [2]
 LDCM0492  CL97 HEK-293T C213(1.01)  LDD1695  [2]
 LDCM0493  CL98 HEK-293T C213(0.94)  LDD1696  [2]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C278(2.85)  LDD1702  [1]
 LDCM0427  Fragment51 HEK-293T C213(1.01)  LDD1631  [2]

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402